PELI1 ORF clone pFN21AE3181

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE3181

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Clone info.

Species

human

Clone Name

pFN21AE3181 Sequence (with vector) Restriction Map

Product ID

FHC21022

ORF Sequence

DNA sequence (1254 bp)
Protein sequence (418 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE3181

Symbol

PELI1

Description

pellino E3 ubiquitin protein ligase 1

Original Clone ID

cp93181

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1254 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 418 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS1876.1 1e-179 100.0 PELI1 CCDS9726.1 1.1e-151 81.4 PELI2 CCDS41675.1 7.5e-130 70.5 PELI3 CCDS31615.1 1.4e-123 66.7 PELI3 CCDS73328.1 2.4e-114 72.6 PELI3 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR006800 4 418 PF04710 Pellino
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) XP_011531296 418 614797 100.0 100.0 XP_016860009 418 614797 100.0 100.0 NP_065702 418 614797 100.0 100.0 NP_067078 420 614798 81.4 100.0 XP_016860010 352 614797 100.0 84.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE3181 Download

>KIEE3181  1254 bp
ATGTTTTCTCCTGATCAAGAAAATCATCCATCTAAAGCACCAGTAAAATATGGTGAACTC
ATTGTCTTAGGGTATAATGGGTCTCTCCCAAATGGCGATAGAGGAAGGAGGAAAAGTAGG
TTTGCTTTGTTTAAAAGACCTAAGGCAAATGGGGTGAAGCCCAGCACTGTGCATATTGCT
TGTACTCCTCAGGCTGCAAAGGCAATAAGCAACAAAGACCAGCATAGCATATCATATACT
TTATCTCGGGCCCAGACTGTGGTGGTTGAATATACTCATGACAGCAACACCGATATGTTT
CAGATTGGCCGGTCGACTGAAAGCCCCATTGATTTTGTAGTAACTGACACGGTTCCTGGA
AGTCAAAGTAATTCTGATACACAGTCAGTACAAAGCACTATATCAAGATTTGCCTGCAGA
ATCATATGTGAACGGAATCCTCCCTTTACAGCACGGATTTATGCTGCAGGATTTGACTCA
TCAAAAAACATCTTTCTTGGGGAGAAGGCTGCCAAATGGAAGACATCAGATGGACAGATG
GATGGCTTGACCACTAATGGTGTTCTTGTGATGCATCCACGCAATGGGTTCACAGAAGAC
TCCAAGCCTGGAATATGGAGAGAAATATCGGTGTGTGGAAATGTATTTAGCCTACGTGAA
ACCAGATCGGCTCAGCAGAGAGGAAAAATGGTGGAAATTGAAACCAATCAGTTACAAGAT
GGCTCGTTAATTGACCTCTGTGGTGCAACATTGTTATGGCGTACTGCAGAAGGCCTTTCC
CACACTCCTACCGTGAAGCATTTAGAAGCTTTAAGACAGGAAATCAATGCAGCACGACCT
CAGTGCCCTGTAGGGTTCAACACACTAGCATTTCCTAGTATGAAGAGGAAAGACGTTGTA
GATGAAAAACAACCATGGGTATATCTAAACTGCGGCCATGTACATGGCTATCATAACTGG
GGAAACAAAGAAGAACGTGATGGAAAAGATCGTGAATGTCCTATGTGTAGGTCTGTTGGT
CCCTATGTTCCTCTGTGGCTTGGATGTGAAGCTGGATTTTATGTGGACGCCGGCCCTCCA
ACCCATGCGTTTAGCCCGTGTGGGCATGTGTGTTCAGAAAAGACAACTGCCTATTGGTCC
CAGATCCCACTTCCTCATGGTACTCATACTTTTCATGCAGCCTGTCCCTTTTGTGCACAT
CAGTTGGCTGGTGAACAAGGCTACATCAGACTTATTTTTCAAGGACCTCTAGAC

ORF Protein

Cloned ORF protein sequence for pFN21AE3181 Download

>KIEE3181  418 aa
MFSPDQENHPSKAPVKYGELIVLGYNGSLPNGDRGRRKSRFALFKRPKANGVKPSTVHIA
CTPQAAKAISNKDQHSISYTLSRAQTVVVEYTHDSNTDMFQIGRSTESPIDFVVTDTVPG
SQSNSDTQSVQSTISRFACRIICERNPPFTARIYAAGFDSSKNIFLGEKAAKWKTSDGQM
DGLTTNGVLVMHPRNGFTEDSKPGIWREISVCGNVFSLRETRSAQQRGKMVEIETNQLQD
GSLIDLCGATLLWRTAEGLSHTPTVKHLEALRQEINAARPQCPVGFNTLAFPSMKRKDVV
DEKQPWVYLNCGHVHGYHNWGNKEERDGKDRECPMCRSVGPYVPLWLGCEAGFYVDAGPP
THAFSPCGHVCSEKTTAYWSQIPLPHGTHTFHAACPFCAHQLAGEQGYIRLIFQGPLD

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE3181 Download

>pFN21AE3181  5959 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGTTTTCTCCTGATCAAGAAAATCATCCATCTAA
AGCACCAGTAAAATATGGTGAACTCATTGTCTTAGGGTATAATGGGTCTCTCCCAAATGG
CGATAGAGGAAGGAGGAAAAGTAGGTTTGCTTTGTTTAAAAGACCTAAGGCAAATGGGGT
GAAGCCCAGCACTGTGCATATTGCTTGTACTCCTCAGGCTGCAAAGGCAATAAGCAACAA
AGACCAGCATAGCATATCATATACTTTATCTCGGGCCCAGACTGTGGTGGTTGAATATAC
TCATGACAGCAACACCGATATGTTTCAGATTGGCCGGTCGACTGAAAGCCCCATTGATTT
TGTAGTAACTGACACGGTTCCTGGAAGTCAAAGTAATTCTGATACACAGTCAGTACAAAG
CACTATATCAAGATTTGCCTGCAGAATCATATGTGAACGGAATCCTCCCTTTACAGCACG
GATTTATGCTGCAGGATTTGACTCATCAAAAAACATCTTTCTTGGGGAGAAGGCTGCCAA
ATGGAAGACATCAGATGGACAGATGGATGGCTTGACCACTAATGGTGTTCTTGTGATGCA
TCCACGCAATGGGTTCACAGAAGACTCCAAGCCTGGAATATGGAGAGAAATATCGGTGTG
TGGAAATGTATTTAGCCTACGTGAAACCAGATCGGCTCAGCAGAGAGGAAAAATGGTGGA
AATTGAAACCAATCAGTTACAAGATGGCTCGTTAATTGACCTCTGTGGTGCAACATTGTT
ATGGCGTACTGCAGAAGGCCTTTCCCACACTCCTACCGTGAAGCATTTAGAAGCTTTAAG
ACAGGAAATCAATGCAGCACGACCTCAGTGCCCTGTAGGGTTCAACACACTAGCATTTCC
TAGTATGAAGAGGAAAGACGTTGTAGATGAAAAACAACCATGGGTATATCTAAACTGCGG
CCATGTACATGGCTATCATAACTGGGGAAACAAAGAAGAACGTGATGGAAAAGATCGTGA
ATGTCCTATGTGTAGGTCTGTTGGTCCCTATGTTCCTCTGTGGCTTGGATGTGAAGCTGG
ATTTTATGTGGACGCCGGCCCTCCAACCCATGCGTTTAGCCCGTGTGGGCATGTGTGTTC
AGAAAAGACAACTGCCTATTGGTCCCAGATCCCACTTCCTCATGGTACTCATACTTTTCA
TGCAGCCTGTCCCTTTTGTGCACATCAGTTGGCTGGTGAACAAGGCTACATCAGACTTAT
TTTTCAAGGACCTCTAGACGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGA
GTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGT
TGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCA
GACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAA
TGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAAT
AAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGG
GAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATC
CTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGT
CAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACT
TTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATG
TATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCT
GTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCA
CGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCC
GAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCC
CGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTG
GTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTA
TGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATC
GGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTT
GATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATG
CCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCT
TCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGC
TCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCT
CGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTAC
ACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCC
TCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAG
CTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACA
TGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTT
TCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGC
GAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCT
CTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCG
TGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCA
AGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACT
ATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTA
ACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTA
ACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCT
TCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTT
TTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGA
TCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCA
TGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGC
ACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATT
AAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTT
ATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGC
CGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCAC
TTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCG
GCGTAGAGGATCGAGATCT