(Circular) (Six-base) MAPSORT of: pFN21AE3181.seq Check: 3201 from: 1 to: 5959 pFN21AE3181 5959 bp With 182 enzymes: SgfI * March 28, 2012 13:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3769 278 Size: 53 83 186 936 2233 2468 Fragments arranged by size: 2468 2233 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2318 3302 1937 Size: 381 984 4594 Fragments arranged by size: 4594 984 381 Acc65I G'GTAC_C Cuts at: 3280 3280 Size: 5959 AclI AA'CG_TT Cuts at: 1563 4086 4459 1563 Size: 2523 373 3063 Fragments arranged by size: 3063 2523 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4022 5406 5674 1632 Size: 2390 1384 268 1917 Fragments arranged by size: 2390 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5762 AflIII A'CryG_T Cuts at: 1203 4858 5835 1203 Size: 3655 977 1327 Fragments arranged by size: 3655 1327 977 AgeI A'CCGG_T Cuts at: 4790 4790 Size: 5959 AhdI GACnn_n'nnGTC Cuts at: 4688 4688 Size: 5959 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5959 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5959 AlwNI CAG_nnn'CTG Cuts at: 3702 5274 3702 Size: 1572 4387 ApaI G_GGCC'C Cuts at: 2257 2257 Size: 5959 ApaLI G'TGCA_C Cuts at: 3198 4016 5172 3198 Size: 818 1156 3985 Fragments arranged by size: 3985 1156 818 ApoI r'AATT_y Cuts at: 1493 3268 3494 3646 5755 1493 Size: 1775 226 152 2109 1697 Fragments arranged by size: 2109 1775 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4513 160 Size: 872 3481 1606 Fragments arranged by size: 3481 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5959 AvaI C'yCGr_G Cuts at: 1870 1943 2250 3284 1870 Size: 73 307 1034 4545 Fragments arranged by size: 4545 1034 307 73 BamHI G'GATC_C Cuts at: 3289 3656 3289 Size: 367 5592 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3280 4735 618 Size: 325 217 627 1493 1455 1842 Fragments arranged by size: 1842 1493 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2257 3278 1391 Size: 866 1021 4072 Fragments arranged by size: 4072 1021 866 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2531 2607 928 Size: 971 632 76 4280 Fragments arranged by size: 4280 971 632 76 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2829 2863 4150 4184 1903 Size: 34 892 34 1287 34 3678 Fragments arranged by size: 3678 1287 892 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3851 5061 3851 Size: 1210 4749 BclI T'GATC_A Cuts at: 1548 2017 1548 Size: 469 5490 BfrBI ATG'CAT Cuts at: 2578 2578 Size: 5959 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4570 136 Size: 107 122 71 631 605 2898 1525 Fragments arranged by size: 2898 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5954 5954 Size: 5959 BlpI GC'TnA_GC Cuts at: 2676 3376 2676 Size: 700 5259 Bme1580I G_kGCm'C Cuts at: 1790 2257 2852 3202 4020 5176 1790 Size: 467 595 350 818 1156 2573 Fragments arranged by size: 2573 1156 818 595 467 350 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5959 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2971 3690 4648 Size: 416 53 83 399 24 1554 719 958 Cuts at: 4648 442 Size: 1753 Fragments arranged by size: 1753 1554 958 719 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5959 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4619 1239 Size: 3380 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4084 4949 5247 4084 Size: 865 298 4796 Fragments arranged by size: 4796 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2091 4622 882 Size: 617 592 2531 2219 Fragments arranged by size: 2531 2219 617 592 BsaAI yAC'GTr Cuts at: 493 1204 2660 3750 493 Size: 711 1456 1090 2702 Fragments arranged by size: 2702 1456 1090 711 BsaBI GATnn'nnATC Cuts at: 5953 5953 Size: 5959 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3072 3766 4148 4783 Size: 53 83 186 936 1539 694 382 635 Cuts at: 4783 275 Size: 1451 Fragments arranged by size: 1539 1451 936 694 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4392 4790 5064 5211 5622 4392 Size: 398 274 147 411 4729 Fragments arranged by size: 4729 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2104 2134 1178 Size: 30 165 30 701 30 5003 Fragments arranged by size: 5003 701 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2181 1186 Size: 995 4964 BseYI C'CCAG_C Cuts at: 2165 5162 5658 2165 Size: 2997 496 2466 Fragments arranged by size: 2997 2466 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5386 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2318 3409 4170 5198 1540 Size: 275 186 317 1091 761 1028 2301 Fragments arranged by size: 2301 1091 1028 761 317 275 186 BsiHKAI G_wGCw'C Cuts at: 3202 4020 4105 5176 3202 Size: 818 85 1071 3985 Fragments arranged by size: 3985 1071 818 85 BsmI GAATG_Cn' Cuts at: 3470 3563 3470 Size: 93 5866 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2257 2852 3202 3278 4020 4105 5176 Size: 399 467 595 350 76 742 85 1071 Cuts at: 5176 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 595 467 399 350 85 76 BspEI T'CCGG_A Cuts at: 5622 5622 Size: 5959 BspHI T'CATG_A Cuts at: 2281 3848 5578 2281 Size: 1567 1730 2662 Fragments arranged by size: 2662 1730 1567 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3314 844 Size: 2470 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3846 1394 Size: 429 2023 3507 Fragments arranged by size: 3507 2023 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2593 4454 4628 65 Size: 1183 1345 1861 174 1396 Fragments arranged by size: 1861 1396 1345 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2313 3074 4603 4790 1700 Size: 110 503 761 1529 187 2869 Fragments arranged by size: 2869 1529 761 503 187 110 BsrGI T'GTAC_A Cuts at: 96 2944 96 Size: 2848 3111 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5959 BssSI C'ACGA_G Cuts at: 4019 5031 4019 Size: 1012 4947 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5959 BstBI TT'CG_AA Cuts at: 4763 4763 Size: 5959 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5959 BstXI CCAn_nnnn'nTGG Cuts at: 2948 2948 Size: 5959 BstYI r'GATC_y Cuts at: 1972 3147 3289 3656 4041 4058 5510 5596 5608 Size: 1175 142 367 385 17 1452 86 12 Cuts at: 5608 5954 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1175 385 367 346 142 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2145 2193 2145 Size: 48 5911 BtgI C'CryG_G Cuts at: 513 1065 2918 513 Size: 552 1853 3554 Fragments arranged by size: 3554 1853 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5220 Fragments arranged by size: 5220 683 56 BtsI GCAGTG_nn' Cuts at: 3475 4269 4289 3475 Size: 794 20 5145 Fragments arranged by size: 5145 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5959 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5924 DraI TTT'AAA Cuts at: 2137 3263 2137 Size: 1126 4833 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4966 809 Size: 507 3650 1802 Fragments arranged by size: 3650 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2311 2938 3406 Size: 54 1425 50 275 18 481 627 468 Cuts at: 3406 4295 5924 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 627 481 468 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3406 1537 Size: 275 1594 4090 Fragments arranged by size: 4090 1594 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2882 3889 1353 Size: 1529 1007 3423 Fragments arranged by size: 3423 1529 1007 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4546 4920 5066 387 Size: 1064 138 2957 374 146 1280 Fragments arranged by size: 2957 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 4022 4619 5406 5674 1239 Size: 393 2390 597 787 268 1524 Fragments arranged by size: 2390 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 3248 3248 Size: 5959 EcoRI G'AATT_C Cuts at: 3268 3646 3268 Size: 378 5581 FspI TGC'GCA Cuts at: 4465 4465 Size: 5959 HaeII r_GCGC'y Cuts at: 1678 5106 1678 Size: 3428 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2372 2404 2811 2843 4600 Size: 32 135 32 800 32 407 32 1757 Cuts at: 4600 4632 4674 4706 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1757 800 407 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2319 3303 3549 669 Size: 988 281 381 984 246 3079 Fragments arranged by size: 3079 988 984 381 281 246 HindIII A'AGCT_T Cuts at: 748 2811 748 Size: 2063 3896 HpaI GTT'AAC Cuts at: 3549 3549 Size: 5959 KpnI G_GTAC'C Cuts at: 3284 3284 Size: 5959 MfeI C'AATT_G Cuts at: 3558 3558 Size: 5959 MluI A'CGCG_T Cuts at: 5835 5835 Size: 5959 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3107 5073 5257 1305 Size: 1802 1966 184 2007 Fragments arranged by size: 2007 1966 1802 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4137 Fragments arranged by size: 4137 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2297 2758 2946 3917 4276 4435 518 Size: 648 1131 461 188 971 359 159 2042 Fragments arranged by size: 2042 1131 971 648 461 359 188 159 MspA1I CmG'CkG Cuts at: 1606 3375 4052 5200 5445 1606 Size: 1769 677 1148 245 2120 Fragments arranged by size: 2120 1769 1148 677 245 NaeI GCC'GGC Cuts at: 3076 3076 Size: 5959 NcoI C'CATG_G Cuts at: 513 1065 2918 513 Size: 552 1853 3554 Fragments arranged by size: 3554 1853 552 NdeI CA'TA_TG Cuts at: 387 2429 387 Size: 2042 3917 NgoMIV G'CCGG_C Cuts at: 3074 3074 Size: 5959 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5959 NotI GC'GGCC_GC Cuts at: 3406 3406 Size: 5959 NsiI A_TGCA'T Cuts at: 2580 2580 Size: 5959 NspI r_CATG'y Cuts at: 1132 1229 3113 3317 4862 1132 Size: 97 1884 204 1545 2229 Fragments arranged by size: 2229 1884 1545 204 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5959 PciI A'CATG_T Cuts at: 4858 4858 Size: 5959 PflMI CCAn_nnn'nTGG Cuts at: 1108 2263 2588 1108 Size: 1155 325 4479 Fragments arranged by size: 4479 1155 325 PfoI T'CCnGG_A Cuts at: 2359 2359 Size: 5959 PmeI GTTT'AAAC Cuts at: 3263 3263 Size: 5959 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4028 4060 5541 5573 4028 Size: 32 1481 32 4414 Fragments arranged by size: 4414 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3248 3248 Size: 5959 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5959 PsiI TTA'TAA Cuts at: 3529 3529 Size: 5959 PspOMI G'GGCC_C Cuts at: 2253 2253 Size: 5959 PstI C_TGCA'G Cuts at: 830 2423 2473 2773 3311 830 Size: 1593 50 300 538 3478 Fragments arranged by size: 3478 1593 538 300 50 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5959 SalI G'TCGA_C Cuts at: 1936 2317 3301 1936 Size: 381 984 4594 Fragments arranged by size: 4594 984 381 SbfI CC_TGCA'GG Cuts at: 3311 3311 Size: 5959 ScaI AGT'ACT Cuts at: 4207 5919 4207 Size: 1712 4247 SfcI C'TryA_G Cuts at: 826 1045 2419 2469 2769 2853 3307 4442 5123 Size: 219 1374 50 300 84 454 1135 681 Cuts at: 5123 5314 826 Size: 191 1471 Fragments arranged by size: 1471 1374 1135 681 454 300 219 191 84 50 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5959 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5959 SmaI CCC'GGG Cuts at: 1872 3286 1872 Size: 1414 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4063 4964 5226 820 Size: 197 926 2120 901 262 1553 Fragments arranged by size: 2120 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3750 493 Size: 3257 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5959 SphI G_CATG'C Cuts at: 1132 3317 1132 Size: 2185 3774 SspI AAT'ATT Cuts at: 5 52 3883 5 Size: 47 3831 2081 Fragments arranged by size: 3831 2081 47 StuI AGG'CCT Cuts at: 1517 2778 1517 Size: 1261 4698 StyI C'CwwG_G Cuts at: 513 1065 2918 3398 513 Size: 552 1853 480 3074 Fragments arranged by size: 3074 1853 552 480 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3986 4003 4156 4341 4786 325 Size: 1255 2406 17 153 185 445 1498 Fragments arranged by size: 2406 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2186 2392 2944 Size: 274 80 33 51 1443 209 206 552 Cuts at: 2944 4205 5917 96 Size: 1261 1712 138 Fragments arranged by size: 1712 1443 1261 552 274 209 206 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2679 2711 3051 3083 5919 5951 2679 Size: 32 340 32 2836 32 2687 Fragments arranged by size: 2836 2687 340 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5959 XbaI T'CTAG_A Cuts at: 3253 3295 3253 Size: 42 5917 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5959 XmaI C'CCGG_G Cuts at: 1870 3284 1870 Size: 1414 4545 XmnI GAAnn'nnTTC Cuts at: 4088 4088 Size: 5959 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3767 276 Size: 53 83 186 936 2233 2468 Fragments arranged by size: 2468 2233 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI PacI PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI