(Linear) (Six-base) MAPSORT of: KIEE3181.seq Check: 304 from: 1 to: 1254 KIEE3181.orf With 182 enzymes: SgfI * March 22, 2012 16:18 .. AccI GT'mk_AC Cuts at: 0 313 1254 Size: 313 941 ApaI G_GGCC'C Cuts at: 0 252 1254 Size: 252 1002 ApaLI G'TGCA_C Cuts at: 0 1193 1254 Size: 1193 61 AvaI C'yCGr_G Cuts at: 0 245 1254 Size: 245 1009 BanII G_rGCy'C Cuts at: 0 252 1254 Size: 252 1002 BbsI GAAGACnn'nnnn_ Cuts at: 0 526 602 1254 Size: 526 76 652 Fragments arranged by size: 652 526 76 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 824 858 1254 Size: 824 34 396 Fragments arranged by size: 824 396 34 BclI T'GATC_A Cuts at: 0 12 1254 Size: 12 1242 BfrBI ATG'CAT Cuts at: 0 573 1254 Size: 573 681 BlpI GC'TnA_GC Cuts at: 0 671 1254 Size: 671 583 Bme1580I G_kGCm'C Cuts at: 0 252 847 1197 1254 Size: 252 595 350 57 Fragments arranged by size: 595 350 252 57 BmrI ACTGGGnnnn_n' Cuts at: 0 966 1254 Size: 966 288 BsaI GGTCTCn'nnnn_ Cuts at: 0 86 1254 Size: 86 1168 BsaAI yAC'GTr Cuts at: 0 655 1254 Size: 655 599 BsaHI Gr'CG_yC Cuts at: 0 1067 1254 Size: 1067 187 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 99 129 1254 Size: 99 30 1125 Fragments arranged by size: 1125 99 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 176 1254 Size: 176 1078 BseYI C'CCAG_C Cuts at: 0 160 1254 Size: 160 1094 BsiEI CG_ry'CG Cuts at: 0 313 1254 Size: 313 941 BsiHKAI G_wGCw'C Cuts at: 0 1197 1254 Size: 1197 57 Bsp1286I G_dGCh'C Cuts at: 0 252 847 1197 1254 Size: 252 595 350 57 Fragments arranged by size: 595 350 252 57 BspHI T'CATG_A Cuts at: 0 276 1254 Size: 276 978 BsrDI GCAATG_nn' Cuts at: 0 588 1254 Size: 588 666 BsrFI r'CCGG_y Cuts at: 0 308 1069 1254 Size: 308 761 185 Fragments arranged by size: 761 308 185 BsrGI T'GTAC_A Cuts at: 0 939 1254 Size: 939 315 BstXI CCAn_nnnn'nTGG Cuts at: 0 943 1254 Size: 943 311 BstYI r'GATC_y Cuts at: 0 1142 1254 Size: 1142 112 Bsu36I CC'TnA_GG Cuts at: 0 140 188 1254 Size: 140 48 1066 Fragments arranged by size: 1066 140 48 BtgI C'CryG_G Cuts at: 0 913 1254 Size: 913 341 DraI TTT'AAA Cuts at: 0 132 1254 Size: 132 1122 EaeI y'GGCC_r Cuts at: 0 306 933 1254 Size: 306 627 321 Fragments arranged by size: 627 321 306 EarI CTCTTCn'nnn_ Cuts at: 0 877 1254 Size: 877 377 EcoO109I rG'GnC_Cy Cuts at: 0 1243 1254 Size: 1243 11 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 367 399 806 838 1254 Size: 367 32 407 32 416 Fragments arranged by size: 416 407 367 32 32 HincII GTy'rAC Cuts at: 0 314 1254 Size: 314 940 HindIII A'AGCT_T Cuts at: 0 806 1254 Size: 806 448 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1102 1254 Size: 1102 152 MslI CAynn'nnrTG Cuts at: 0 292 753 941 1254 Size: 292 461 188 313 Fragments arranged by size: 461 313 292 188 NaeI GCC'GGC Cuts at: 0 1071 1254 Size: 1071 183 NcoI C'CATG_G Cuts at: 0 913 1254 Size: 913 341 NdeI CA'TA_TG Cuts at: 0 424 1254 Size: 424 830 NgoMIV G'CCGG_C Cuts at: 0 1069 1254 Size: 1069 185 NsiI A_TGCA'T Cuts at: 0 575 1254 Size: 575 679 NspI r_CATG'y Cuts at: 0 1108 1254 Size: 1108 146 PflMI CCAn_nnn'nTGG Cuts at: 0 258 583 1254 Size: 258 325 671 Fragments arranged by size: 671 325 258 PfoI T'CCnGG_A Cuts at: 0 354 1254 Size: 354 900 PpuMI rG'GwC_Cy Cuts at: 0 1243 1254 Size: 1243 11 PspOMI G'GGCC_C Cuts at: 0 248 1254 Size: 248 1006 PstI C_TGCA'G Cuts at: 0 418 468 768 1254 Size: 418 50 300 486 Fragments arranged by size: 486 418 300 50 SalI G'TCGA_C Cuts at: 0 312 1254 Size: 312 942 SfcI C'TryA_G Cuts at: 0 414 464 764 848 1254 Size: 414 50 300 84 406 Fragments arranged by size: 414 406 300 84 50 StuI AGG'CCT Cuts at: 0 773 1254 Size: 773 481 StyI C'CwwG_G Cuts at: 0 913 1254 Size: 913 341 TatI w'GTAC_w Cuts at: 0 181 387 939 1254 Size: 181 206 552 315 Fragments arranged by size: 552 315 206 181 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 674 706 1046 1078 1254 Size: 674 32 340 32 176 Fragments arranged by size: 674 340 176 32 32 XbaI T'CTAG_A Cuts at: 0 1248 1254 Size: 1248 6 Enzymes that do cut: AccI ApaI ApaLI AvaI BanII BbsI BcgI BclI BfrBI BlpI Bme1580I BmrI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspHI BsrDI BsrFI BsrGI BstXI BstYI Bsu36I BtgI DraI EaeI EarI EcoO109I Hin4I HincII HindIII MmeI MslI NaeI NcoI NdeI NgoMIV NsiI NspI PflMI PfoI PpuMI PspOMI PstI SalI SfcI StuI StyI TatI TstI XbaI Enzymes that do not cut: AarI AatII Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApoI AscI AseI AsiSI AvrII BaeI BamHI BanI BbeI BbvCI BciVI BglI BglII BmgBI BmtI BplI BpmI Bpu10I BpuEI BsaBI BsaWI BsgI BsiWI BsmI BsmBI BspEI BspMI BsrBI BssHII BssSI BstAPI BstBI BstEII BstZ17I BtgZI BtsI ClaI CspCI DraIII DrdI EagI EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HaeII HpaI KasI KpnI MfeI MluI MscI MspA1I NarI NheI NotI NruI PacI PasI PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI PvuII RsrII SacI SacII SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI SwaI TaqII Tth111I XcmI XhoI XmaI XmnI ZraI