################################## # NO CUT ENZYMES ( 133 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AflII AflIII AgeI AhdI AlwNI Aor51HI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BanI BbeI BbrPI BbvCI BciVI BcnI BfrI BglI BglII BlnI BpmI BsaBI BsaWI BseAI BsgI BsiWI BsmBI BsmI BspDI BspEI BspLU11I BspMI BsrBI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I CfoI ClaI CpoI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV FauI FseI FspI HaeII HhaI HinP1I HpaI KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MspA1I MunI MvnI NarI NciI NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RsrII SacI SacII SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI SwaI Tsp45I Tth111I XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 62 enzymes) # ################################### AccI 312 GTCGAC GT^[AC][GT]AC AciI 932 GCGG CCGC(-3/-1) AcyI 1066 GACGCC G[GA]^CG[CT]C Alw44I 1193 GTGCAC G^TGCAC AlwI 1143 GATCC GGATC(4/5) ApaI 248 GGGCCC GGGCC^C ApaLI 1193 GTGCAC G^TGCAC AspHI 1193 GTGCAC G[AT]GC[AT]^C AvaI 245 CTCGGG C^[CT]CG[GA]G BanII 248 GGGCCC G[GA]GC[CT]^C BcgI 835 CGACCTCAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 12 TGATCA T^GATCA BlpI 670 GCTCAGC GC^T[ACGT]AGC Bpu1102I 670 GCTCAGC GC^T[ACGT]AGC BsaAI 653 TACGTG [CT]AC^GT[GA] BsaHI 1066 GACGCC G[GA]^CG[CT]C BsaI 80 GGTCTC GGTCTC(1/5) BsaJI 913 CCATGG C^C[ACGT][ACGT]GG BseRI 185 CTCCTC GAGGAG(10/8) BsiEI 310 CGGTCG CG[GA][CT]^CG BsiHKAI 1193 GTGCAC G[AT]GC[AT]^C BsmAI 81 GTCTC GTCTC(1/5) BsoBI 245 CTCGGG C^[CT]CG[GA]G Bsp1407I 939 TGTACA T^GTACA BspHI 276 TCATGA T^CATGA BsrDI 581 GCAATG GCAATG(2/0) BsrGI 939 TGTACA T^GTACA BstXI 936 CCATGTACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 1142 AGATCC [GA]^GATC[CT] CelII 670 GCTCAGC GC^T[ACGT]AGC DraI 130 TTTAAA TTT^AAA DraII 1242 AGGACCT [GA]G^G[ACGT]CC[CT] DsaI 913 CCATGG C^C[GA][CT]GG EarI 882 GAAGAG CTCTTC(1/4) EcoO109I 1242 AGGACCT [GA]G^G[ACGT]CC[CT] EcoT14I 913 CCATGG C^C[AT][AT]GG EcoT22I 571 ATGCAT ATGCA^T FbaI 12 TGATCA T^GATCA HgaI 1066 GACGC GACGC(5/10) Hin1I 1066 GACGCC G[GA]^CG[CT]C HincII 312 GTCGAC GT[CT]^[GA]AC HindII 312 GTCGAC GT[CT]^[GA]AC HindIII 806 AAGCTT A^AGCTT Ksp632I 882 GAAGAG CTCTTC(1/4) MflI 1142 AGATCC [GA]^GATC[CT] MwoI 193 GCTGCAAAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NaeI 1069 GCCGGC GCC^GGC NcoI 913 CCATGG C^CATGG NdeI 423 CATATG CA^TATG NgoMIV 1069 GCCGGC G^CCGGC NsiI 571 ATGCAT ATGCA^T NspI 1104 GCATGT [GA]CATG^[CT] PpuMI 1242 AGGACCT [GA]G^G[AT]CC[CT] RcaI 276 TCATGA T^CATGA SalI 312 GTCGAC G^TCGAC SspBI 939 TGTACA T^GTACA StuI 771 AGGCCT AGG^CCT StyI 913 CCATGG C^C[AT][AT]GG TaqI 313 TCGA T^CGA TthHB8I 313 TCGA T^CGA XbaI 1248 TCTAGA T^CTAGA XhoII 1142 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AluI 807 AGCT AG^CT 1050 AGCT AocI 139 CCTAAGG CC^T[ACGT]AGG 187 CCTCAGG BbsI 519 GAAGAC GAAGAC(2/6) 595 GAAGAC BpuAI 519 GAAGAC GAAGAC(2/6) 595 GAAGAC BsiYI 252 CCAGACTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 577 CCACGCAATGG BslI 252 CCAGACTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 577 CCACGCAATGG BsrFI 308 GCCGGT [GA]^CCGG[CT] 1069 GCCGGC BsrI 956 ACTGG ACTGG(1/-1) 40 CCAGT BssKI 355 CCTGG ^CC[ACGT]GG 607 CCTGG BstNI 355 CCTGG CC^[AT]GG 607 CCTGG Bsu36I 139 CCTAAGG CC^T[ACGT]AGG 187 CCTCAGG Cac8I 412 GCCTGC GC[ACGT]^[ACGT]GC 1069 GCCGGC Cfr10I 308 GCCGGT [GA]^CCGG[CT] 1069 GCCGGC EaeI 306 TGGCCG [CT]^GGCC[GA] 933 CGGCCA Eco81I 139 CCTAAGG CC^T[ACGT]AGG 187 CCTCAGG EcoRII 355 CCTGG ^CC[AT]GG 607 CCTGG HapII 309 CCGG C^CGG 1070 CCGG HpaII 309 CCGG C^CGG 1070 CCGG MaeIII 340 GTAAC ^GT[ACGT]AC 711 GTTAC MspI 309 CCGG C^CGG 1070 CCGG MvaI 355 CCTGG CC^[AT]GG 607 CCTGG PflMI 252 CCAGACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 577 CCACGCAATGG PleI 475 GACTC GAGTC(4/5) 598 GACTC ScrFI 355 CCTGG CC^[ACGT]GG 607 CCTGG SfaNI 570 GATGC GCATC(5/9) 573 GCATC TfiI 419 GAATC G^A[AT]TC 435 GAATC TspRI 165 CACTG CA[GC]TG[ACGT][ACGT]^ 841 CAGTG Van91I 252 CCAGACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 577 CCACGCAATGG ######################################### # 3 OR MORE CUT ENZYMES ( 35 enzymes) # ######################################### AfaI AvaII BbvI BfaI BmyI BsmFI Bsp1286I BstF5I Cfr13I DdeI DpnII Fnu4HI FokI HaeIII HinfI HphI ItaI MaeI MaeII MboI MboII MnlI MseI MslI NdeII NlaIII NlaIV PstI RsaI Sau3AI Sau96I SfcI Tru9I TseI Tsp509I