ALOX12B ORF clone pFN21AE2059

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE2059

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Clone info.

Species

human

Clone Name

pFN21AE2059 Sequence (with vector) Restriction Map

Product ID

FHC25882

ORF Sequence

DNA sequence (2103 bp)
Protein sequence (701 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB590845]

Gene

KIEE2059

Symbol

ALOX12B

Alias : 12R-LOX, ARCI2

Description

arachidonate 12-lipoxygenase, 12R type

Original Clone ID

cs02959

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 2103 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 701 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS11129.1 0 100.0 ALOX12B CCDS11130.1 1.5e-139 54.1 ALOXE3 CCDS54084.1 1.7e-139 54.1 ALOXE3 CCDS11128.1 3.7e-130 48.4 ALOX15B CCDS7212.1 2.6e-96 39.9 ALOX5 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR001885 11 28 PR00467 Lipoxygenase IPR001885 61 80 PR00467 Lipoxygenase IPR001885 138 152 PR00467 Lipoxygenase IPR013819 375 392 PR00087 Lipoxygenase IPR013819 393 410 PR00087 Lipoxygenase IPR013819 413 433 PR00087 Lipoxygenase IPR001885 458 480 PR00467 Lipoxygenase IPR001885 618 635 PR00467 Lipoxygenase HMMPfam IPR001024 3 114 PF01477 Lipoxygenase IPR013819 245 692 PF00305 Lipoxygenase HMMSmart IPR001024 2 116 SM00308 Lipoxygenase ProfileScan IPR001024 2 119 PS50095 Lipoxygenase IPR013819 119 701 PS51393 Lipoxygenase
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_001130 701 242100,603741 100.0 100.0 NP_067641 711 242100,606545,607206 54.1 100.0 NP_001159432 843 242100,606545,607206 54.1 100.0 NP_001132 676 603697 48.4 100.0 XP_016880410 615 242100,606545,607206 52.3 83.5 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE2059 Download

>KIEE2059  2103 bp
ATGGCCACCTACAAAGTCAGGGTGGCCACAGGCACCGACCTCTTGTCGGGAACACGGGAC
TCCATCTCACTGACCATTGTGGGGACACAAGGAGAGAGCCATAAGCAGCTGCTGAACCAC
TTTGGGAGAGACTTTGCAACTGGGGCGGTGGGCCAGTACACCGTGCAGTGCCCTCAGGAC
CTGGGTGAGCTCATCATCATCCGCCTGCACAAAGAGCGGTACGCCTTCTTCCCCAAGGAC
CCTTGGTACTGCAACTATGTGCAGATCTGTGCCCCCAACGGCCGTATCTACCACTTCCCC
GCCTACCAGTGGATGGATGGCTACGAGACCCTGGCACTCCGGGAGGCCACAGGAAAGACA
ACAGCAGATGACTCGCTCCCCGTCCTCCTGGAGCACAGAAAAGAGGAGATCAGAGCCAAG
CAGGACTTCTACCACTGGCGAGTCTTTCTTCCTGGCCTGCCCAGCTATGTGCACATTCCC
AGTTACCGCCCTCCGGTGCGGAGGCATCGCAACCCCAACCGGCCTGAGTGGAATGGCTAT
ATTCCGGGATTCCCAATTCTCATCAACTTTAAGGCCACCAAGTTCCTGAACTTAAATCTC
CGCTACTCCTTCCTCAAGACGGCCTCCTTCTTCGTCCGCCTGGGGCCCATGGCACTGGCT
TTCAAAGTCCGCGGCCTGTTGGACTGCAAACATTCGTGGAAGAGGCTGAAGGACATTAGG
AAAATTTTCCCTGGCAAGAAATCTGTCGTCTCCGAGTACGTGGCCGAGCACTGGGCAGAG
GACACCTTCTTTGGGTACCAGTACCTCAACGGCGTCAACCCCGGCCTGATCCGCCGCTGC
ACGCGGATCCCAGACAAGTTCCCCGTCACAGACGACATGGTGGCTCCGTTCCTGGGCGAG
GGAACGTGCTTGCAAGCGGAGCTGGAGAAGGGGAACATTTACCTGGCCGACTACCGCATC
ATGGAGGGCATCCCCACCGTGGAGCTCAGCGGCCGGAAGCAGCACCACTGCGCCCCCCTC
TGCCTGCTGCACTTTGGACCCGAGGGCAAGATGATGCCCATCGCCATCCAGCTCAGCCAG
ACCCCTGGGCCAGATTGCCCCATCTTCCTGCCCAGTGATTCTGAGTGGGACTGGCTGCTA
GCCAAGACGTGGGTACGCTATGCGGAGTTCTACAGCCACGAGGCCATTGCCCACCTGCTG
GAGACACACCTCATTGCTGAGGCCTTCTGCCTGGCCTTGCTGAGGAACCTGCCCATGTGC
CACCCCCTCTACAAGCTCCTCATCCCCCATACCCGATACACCGTCCAGATCAACAGCATT
GGCCGGGCCGTTCTCCTCAATGAGGGGGGGCTCTCTGCCAAGGGCATGTCCCTGGGCGTG
GAAGGCTTTGCTGGGGTGATGGTACGGGCTCTGTCGGAGCTCACCTATGACAGCCTCTAC
CTCCCCAATGACTTTGTGGAGCGTGGGGTCCAGGACCTGCCTGGATATTACTACCGCGAT
GACAGCTTGGCGGTGTGGAATGCACTGGAGAAGTATGTGACGGAGATCATCACCTATTAT
TACCCGAGTGACGCAGCCGTGGAGGGTGATCCGGAATTGCAGTCTTGGGTGCAGGAAATA
TTTAAAGAGTGCCTCCTGGGGCGGGAGAGCTCAGGCTTCCCTAGGTGCTTGCGAACCGTG
CCTGAGCTGATCCGATATGTCACTATAGTCATCTACACCTGCTCTGCCAAGCACGCTGCT
GTCAACACAGGCCAGATGGAGTTCACCGCCTGGATGCCCAACTTCCCAGCGTCCATGCGG
AATCCACCGATTCAGACTAAGGGGCTGACCACTCTGGAGACCTTCATGGACACGTTGCCG
GATGTGAAGACCACGTGCATCACGCTGCTGGTGCTCTGGACCCTCAGCCGAGAGCCTGAC
GACAGGCGGCCCCTGGGACACTTCCCGGACATTCACTTCGTGGAGGAGGCCCCGCGGAGG
AGCATAGAGGCGTTCCGCCAGCGCCTGAACCAGATCTCACACGACATCCGCCAGCGCAAC
AAGTGCCTTCCCATCCCCTACTACTACCTGGACCCGGTGCTGATTGAGAACAGCATTTCT
ATT

ORF Protein

Cloned ORF protein sequence for pFN21AE2059 Download

>KIEE2059  701 aa
MATYKVRVATGTDLLSGTRDSISLTIVGTQGESHKQLLNHFGRDFATGAVGQYTVQCPQD
LGELIIIRLHKERYAFFPKDPWYCNYVQICAPNGRIYHFPAYQWMDGYETLALREATGKT
TADDSLPVLLEHRKEEIRAKQDFYHWRVFLPGLPSYVHIPSYRPPVRRHRNPNRPEWNGY
IPGFPILINFKATKFLNLNLRYSFLKTASFFVRLGPMALAFKVRGLLDCKHSWKRLKDIR
KIFPGKKSVVSEYVAEHWAEDTFFGYQYLNGVNPGLIRRCTRIPDKFPVTDDMVAPFLGE
GTCLQAELEKGNIYLADYRIMEGIPTVELSGRKQHHCAPLCLLHFGPEGKMMPIAIQLSQ
TPGPDCPIFLPSDSEWDWLLAKTWVRYAEFYSHEAIAHLLETHLIAEAFCLALLRNLPMC
HPLYKLLIPHTRYTVQINSIGRAVLLNEGGLSAKGMSLGVEGFAGVMVRALSELTYDSLY
LPNDFVERGVQDLPGYYYRDDSLAVWNALEKYVTEIITYYYPSDAAVEGDPELQSWVQEI
FKECLLGRESSGFPRCLRTVPELIRYVTIVIYTCSAKHAAVNTGQMEFTAWMPNFPASMR
NPPIQTKGLTTLETFMDTLPDVKTTCITLLVLWTLSREPDDRRPLGHFPDIHFVEEAPRR
SIEAFRQRLNQISHDIRQRNKCLPIPYYYLDPVLIENSISI

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE2059 Download

>pFN21AE2059  6808 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCCACCTACAAAGTCAGGGTGGCCACAGGCAC
CGACCTCTTGTCGGGAACACGGGACTCCATCTCACTGACCATTGTGGGGACACAAGGAGA
GAGCCATAAGCAGCTGCTGAACCACTTTGGGAGAGACTTTGCAACTGGGGCGGTGGGCCA
GTACACCGTGCAGTGCCCTCAGGACCTGGGTGAGCTCATCATCATCCGCCTGCACAAAGA
GCGGTACGCCTTCTTCCCCAAGGACCCTTGGTACTGCAACTATGTGCAGATCTGTGCCCC
CAACGGCCGTATCTACCACTTCCCCGCCTACCAGTGGATGGATGGCTACGAGACCCTGGC
ACTCCGGGAGGCCACAGGAAAGACAACAGCAGATGACTCGCTCCCCGTCCTCCTGGAGCA
CAGAAAAGAGGAGATCAGAGCCAAGCAGGACTTCTACCACTGGCGAGTCTTTCTTCCTGG
CCTGCCCAGCTATGTGCACATTCCCAGTTACCGCCCTCCGGTGCGGAGGCATCGCAACCC
CAACCGGCCTGAGTGGAATGGCTATATTCCGGGATTCCCAATTCTCATCAACTTTAAGGC
CACCAAGTTCCTGAACTTAAATCTCCGCTACTCCTTCCTCAAGACGGCCTCCTTCTTCGT
CCGCCTGGGGCCCATGGCACTGGCTTTCAAAGTCCGCGGCCTGTTGGACTGCAAACATTC
GTGGAAGAGGCTGAAGGACATTAGGAAAATTTTCCCTGGCAAGAAATCTGTCGTCTCCGA
GTACGTGGCCGAGCACTGGGCAGAGGACACCTTCTTTGGGTACCAGTACCTCAACGGCGT
CAACCCCGGCCTGATCCGCCGCTGCACGCGGATCCCAGACAAGTTCCCCGTCACAGACGA
CATGGTGGCTCCGTTCCTGGGCGAGGGAACGTGCTTGCAAGCGGAGCTGGAGAAGGGGAA
CATTTACCTGGCCGACTACCGCATCATGGAGGGCATCCCCACCGTGGAGCTCAGCGGCCG
GAAGCAGCACCACTGCGCCCCCCTCTGCCTGCTGCACTTTGGACCCGAGGGCAAGATGAT
GCCCATCGCCATCCAGCTCAGCCAGACCCCTGGGCCAGATTGCCCCATCTTCCTGCCCAG
TGATTCTGAGTGGGACTGGCTGCTAGCCAAGACGTGGGTACGCTATGCGGAGTTCTACAG
CCACGAGGCCATTGCCCACCTGCTGGAGACACACCTCATTGCTGAGGCCTTCTGCCTGGC
CTTGCTGAGGAACCTGCCCATGTGCCACCCCCTCTACAAGCTCCTCATCCCCCATACCCG
ATACACCGTCCAGATCAACAGCATTGGCCGGGCCGTTCTCCTCAATGAGGGGGGGCTCTC
TGCCAAGGGCATGTCCCTGGGCGTGGAAGGCTTTGCTGGGGTGATGGTACGGGCTCTGTC
GGAGCTCACCTATGACAGCCTCTACCTCCCCAATGACTTTGTGGAGCGTGGGGTCCAGGA
CCTGCCTGGATATTACTACCGCGATGACAGCTTGGCGGTGTGGAATGCACTGGAGAAGTA
TGTGACGGAGATCATCACCTATTATTACCCGAGTGACGCAGCCGTGGAGGGTGATCCGGA
ATTGCAGTCTTGGGTGCAGGAAATATTTAAAGAGTGCCTCCTGGGGCGGGAGAGCTCAGG
CTTCCCTAGGTGCTTGCGAACCGTGCCTGAGCTGATCCGATATGTCACTATAGTCATCTA
CACCTGCTCTGCCAAGCACGCTGCTGTCAACACAGGCCAGATGGAGTTCACCGCCTGGAT
GCCCAACTTCCCAGCGTCCATGCGGAATCCACCGATTCAGACTAAGGGGCTGACCACTCT
GGAGACCTTCATGGACACGTTGCCGGATGTGAAGACCACGTGCATCACGCTGCTGGTGCT
CTGGACCCTCAGCCGAGAGCCTGACGACAGGCGGCCCCTGGGACACTTCCCGGACATTCA
CTTCGTGGAGGAGGCCCCGCGGAGGAGCATAGAGGCGTTCCGCCAGCGCCTGAACCAGAT
CTCACACGACATCCGCCAGCGCAACAAGTGCCTTCCCATCCCCTACTACTACCTGGACCC
GGTGCTGATTGAGAACAGCATTTCTATTGTTTAAACGAATTCGGGCTCGGTACCCGGGGA
TCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGG
AAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCG
CTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAG
TGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATA
AGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGG
GAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTC
TAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTC
ATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCA
GGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACAT
TCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAA
AGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTT
TGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAG
TTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGT
TTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCG
GTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAG
AATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTA
AGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTG
ACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTA
ACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGAC
ACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTT
ACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCA
CTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAG
CGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTA
GTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAG
ATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAAC
CGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAG
GAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGC
TGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTC
AGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCC
TCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTT
CGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCG
TTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTAT
CCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAG
CCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGT
GGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGC
CAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTA
GCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAG
ATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGA
TTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATA
CAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTT
CAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGG
TTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCT
GTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGG
TAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACG
ATGCGTCCGGCGTAGAGGATCGAGATCT