(Circular) (Six-base) MAPSORT of: pFN21AE2059.seq Check: 2439 from: 1 to: 6808 pFN21AE2059 6808 bp With 182 enzymes: SgfI * August 16, 2010 14:47 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3207 3731 3207 Size: 524 6284 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4618 278 Size: 53 83 186 936 3082 2468 Fragments arranged by size: 3082 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4151 1937 Size: 2214 4594 Acc65I G'GTAC_C Cuts at: 2799 4129 2799 Size: 1330 5478 AclI AA'CG_TT Cuts at: 1563 4935 5308 1563 Size: 3372 373 3063 Fragments arranged by size: 3372 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2732 4871 6255 6523 1632 Size: 1100 2139 1384 268 1917 Fragments arranged by size: 2139 1917 1384 1100 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6611 AflIII A'CryG_T Cuts at: 1203 3855 5707 6684 1203 Size: 2652 1852 977 1327 Fragments arranged by size: 2652 1852 1327 977 AgeI A'CCGG_T Cuts at: 5639 5639 Size: 6808 AhdI GACnn_n'nnGTC Cuts at: 2047 5537 2047 Size: 3490 3318 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6808 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2279 2289 3074 3905 1184 Size: 1095 10 785 831 4087 Fragments arranged by size: 4087 1095 831 785 10 AlwNI CAG_nnn'CTG Cuts at: 2116 2185 3481 4009 4551 6123 2116 Size: 69 1296 528 542 1572 2801 Fragments arranged by size: 2801 1572 1296 542 528 69 ApaI G_GGCC'C Cuts at: 2652 2652 Size: 6808 ApaLI G'TGCA_C Cuts at: 2474 4865 6021 2474 Size: 2391 1156 3261 Fragments arranged by size: 3261 2391 1156 ApoI r'AATT_y Cuts at: 1493 2727 4117 4343 4495 6604 1493 Size: 1234 1390 226 152 2109 1697 Fragments arranged by size: 2109 1697 1390 1234 226 152 AseI AT'TA_AT Cuts at: 160 1032 5362 160 Size: 872 4330 1606 Fragments arranged by size: 4330 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6808 AvaI C'yCGr_G Cuts at: 1870 1943 3044 3568 4133 1870 Size: 73 1101 524 565 4545 Fragments arranged by size: 4545 1101 565 524 73 AvrII C'CTAG_G Cuts at: 3665 3665 Size: 6808 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2272 2305 2272 Size: 33 6775 BamHI G'GATC_C Cuts at: 2850 4138 4505 2850 Size: 1288 367 5153 Fragments arranged by size: 5153 1288 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2036 2799 4129 5584 618 Size: 325 217 627 249 763 1330 1455 1842 Fragments arranged by size: 1842 1455 1330 763 627 325 249 217 BanII G_rGCy'C Cuts at: 1391 2196 2652 2991 3357 3415 3426 3656 4127 Size: 805 456 339 366 58 11 230 471 Cuts at: 4127 1391 Size: 4072 Fragments arranged by size: 4072 805 471 456 366 339 230 58 11 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3878 928 Size: 971 1979 3858 Fragments arranged by size: 3858 1979 971 BbvCI CC'TCA_GC Cuts at: 3222 3245 3908 3222 Size: 23 663 6122 Fragments arranged by size: 6122 663 23 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2358 2392 3173 3207 4999 5033 1903 Size: 34 421 34 781 34 1792 34 3678 Fragments arranged by size: 3678 1792 781 421 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4700 5910 4700 Size: 1210 5598 BclI T'GATC_A Cuts at: 1548 1548 Size: 6808 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5419 136 Size: 107 122 71 631 605 3747 1525 Fragments arranged by size: 3747 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2268 4017 6803 2268 Size: 1749 2786 2273 Fragments arranged by size: 2786 2273 1749 BlpI GC'TnA_GC Cuts at: 2990 3077 4225 2990 Size: 87 1148 5573 Fragments arranged by size: 5573 1148 87 Bme1580I G_kGCm'C Cuts at: 1790 2177 2278 2478 2652 4869 6025 1790 Size: 387 101 200 174 2217 1156 2573 Fragments arranged by size: 2573 2217 1156 387 200 174 101 BmgBI CAC'GTC Cuts at: 1318 3153 1318 Size: 1835 4973 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2154 2478 2785 Size: 416 53 83 399 24 737 324 307 Cuts at: 2785 3111 4539 5497 442 Size: 326 1428 958 1753 Fragments arranged by size: 1753 1428 958 737 416 399 326 324 307 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3146 1056 Size: 2090 4718 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2414 2948 3224 3551 3860 5468 1239 Size: 1175 534 276 327 309 1608 2579 Fragments arranged by size: 2579 1608 1175 534 327 309 276 Bpu10I CC'TnA_GC Cuts at: 3222 3245 3655 3687 3908 3222 Size: 23 410 32 221 6122 Fragments arranged by size: 6122 410 221 32 23 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2603 4933 5798 6096 2603 Size: 2330 865 298 3315 Fragments arranged by size: 3315 2330 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2324 3836 5471 882 Size: 617 825 1512 1635 2219 Fragments arranged by size: 2219 1635 1512 825 617 BsaAI yAC'GTr Cuts at: 493 1204 2764 3879 4599 493 Size: 711 1560 1115 720 2702 Fragments arranged by size: 2702 1560 1115 720 711 BsaBI GATnn'nnATC Cuts at: 3062 6802 3062 Size: 3740 3068 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2817 4615 4997 5632 Size: 53 83 186 936 1284 1798 382 635 Cuts at: 5632 275 Size: 1451 Fragments arranged by size: 1798 1451 1284 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2497 3595 5241 5639 5913 6060 6471 2497 Size: 1098 1646 398 274 147 411 2834 Fragments arranged by size: 2834 1646 1098 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6583 Fragments arranged by size: 6583 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2423 3272 3329 3983 3997 1186 Size: 1237 849 57 654 14 3997 Fragments arranged by size: 3997 1237 849 654 57 14 BseYI C'CCAG_C Cuts at: 2465 3395 3790 6011 6507 2465 Size: 930 395 2221 496 2766 Fragments arranged by size: 2766 2221 930 496 395 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2189 2195 2285 2827 3017 3635 1163 Size: 573 453 6 90 542 190 618 4336 Fragments arranged by size: 4336 618 573 542 453 190 90 6 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2287 2998 4258 5019 6047 1540 Size: 275 186 286 711 1260 761 1028 2301 Fragments arranged by size: 2301 1260 1028 761 711 286 275 186 BsiHKAI G_wGCw'C Cuts at: 2196 2400 2478 2775 2991 3426 3656 3900 4869 Size: 204 78 297 216 435 230 244 969 Cuts at: 4869 4954 6025 2196 Size: 85 1071 2979 Fragments arranged by size: 2979 1071 969 435 297 244 230 216 204 85 78 BsmI GAATG_Cn' Cuts at: 3529 4319 4412 3529 Size: 790 93 5925 Fragments arranged by size: 5925 790 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2758 2758 Size: 6808 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2177 2196 2278 2400 2478 2652 2775 Size: 399 387 19 82 122 78 174 123 Cuts at: 2775 2991 3357 3415 3426 3656 3900 4127 4869 Size: 216 366 58 11 230 244 227 742 Cuts at: 4869 4954 6025 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 387 366 244 230 227 216 174 123 122 85 82 78 58 19 11 BspEI T'CCGG_A Cuts at: 3595 6471 3595 Size: 2876 3932 BspHI T'CATG_A Cuts at: 4697 6427 4697 Size: 1730 5078 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3207 3261 3489 3731 4163 844 Size: 2363 54 228 242 432 3489 Fragments arranged by size: 3489 2363 432 242 228 54 BsrBI CCG'CTC Cuts at: 1394 1823 2221 4695 1394 Size: 429 398 2474 3507 Fragments arranged by size: 3507 2474 429 398 BsrDI GCAATG_nn' Cuts at: 65 1248 3189 3216 5303 5477 65 Size: 1183 1941 27 2087 174 1396 Fragments arranged by size: 2087 1941 1396 1183 174 27 BsrFI r'CCGG_y Cuts at: 1700 1810 2523 5452 5639 1700 Size: 110 713 2929 187 2869 Fragments arranged by size: 2929 2869 713 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6808 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6808 BssSI C'ACGA_G Cuts at: 3182 4868 5880 3182 Size: 1686 1012 4110 Fragments arranged by size: 4110 1686 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 4047 794 Size: 3253 3555 BstBI TT'CG_AA Cuts at: 5612 5612 Size: 6808 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6808 BstXI CCAn_nnnn'nTGG Cuts at: 2659 3089 2659 Size: 430 6378 BstYI r'GATC_y Cuts at: 1972 2268 2850 4017 4138 4505 4890 4907 6359 Size: 296 582 1167 121 367 385 17 1452 Cuts at: 6359 6445 6457 6803 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1167 582 385 367 346 296 121 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2178 2178 Size: 6808 BtgI C'CryG_G Cuts at: 513 1065 2004 2652 2674 2982 3582 3977 513 Size: 552 939 648 22 308 600 395 3344 Fragments arranged by size: 3344 939 648 600 552 395 308 22 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2495 3049 3516 487 Size: 683 56 1269 554 467 3779 Fragments arranged by size: 3779 1269 683 554 467 56 BtsI GCAGTG_nn' Cuts at: 2177 3010 4324 5118 5138 2177 Size: 833 1314 794 20 3847 Fragments arranged by size: 3847 1314 833 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6808 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6773 DraI TTT'AAA Cuts at: 3628 4112 3628 Size: 484 6324 DraIII CAC_nnn'GTG Cuts at: 3964 3964 Size: 6808 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5815 809 Size: 507 4499 1802 Fragments arranged by size: 4499 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2028 2284 Size: 54 1425 50 275 18 177 21 256 Cuts at: 2284 2766 2949 2995 3325 4255 5144 6773 8 Size: 482 183 46 330 930 889 1629 43 Fragments arranged by size: 1629 1425 930 889 482 330 275 256 183 177 54 50 46 43 21 18 EagI C'GGCC_G Cuts at: 1537 1812 2284 2995 4255 1537 Size: 275 472 711 1260 4090 Fragments arranged by size: 4090 1260 711 472 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2699 4738 1353 Size: 1346 2039 3423 Fragments arranged by size: 3423 2039 1346 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2195 2630 2825 3989 4022 5395 Size: 1064 138 606 435 195 1164 33 1373 Cuts at: 5395 5769 5915 387 Size: 374 146 1280 Fragments arranged by size: 1373 1280 1164 1064 606 435 374 195 146 138 33 EcoICRI GAG'CTC Cuts at: 2194 2989 3424 3654 2194 Size: 795 435 230 5348 Fragments arranged by size: 5348 795 435 230 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2414 2732 2948 3224 3551 3860 4871 Size: 393 782 318 216 276 327 309 1011 Cuts at: 4871 5468 6255 6523 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1011 787 782 597 393 327 318 309 276 268 216 EcoNI CCTnn'n_nnAGG Cuts at: 2900 3244 3344 2900 Size: 344 100 6364 Fragments arranged by size: 6364 344 100 EcoO109I rG'GnC_Cy Cuts at: 2182 2242 2648 3478 3973 2182 Size: 60 406 830 495 5017 Fragments arranged by size: 5017 830 495 406 60 EcoRI G'AATT_C Cuts at: 4117 4495 4117 Size: 378 6430 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2446 2446 Size: 6808 FspI TGC'GCA Cuts at: 5314 5314 Size: 6808 HaeII r_GCGC'y Cuts at: 1678 4009 5955 1678 Size: 2331 1946 2531 Fragments arranged by size: 2531 2331 1946 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2033 2054 2065 2086 3291 Size: 32 135 32 461 21 11 21 1205 Cuts at: 3291 3323 5449 5481 5523 5555 1373 Size: 32 2126 32 42 32 2626 Fragments arranged by size: 2626 2126 1205 461 135 42 32 32 32 32 32 21 21 11 HincII GTy'rAC Cuts at: 669 1657 1938 2821 3748 4152 4398 669 Size: 988 281 883 927 404 246 3079 Fragments arranged by size: 3079 988 927 883 404 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6808 HpaI GTT'AAC Cuts at: 4398 4398 Size: 6808 KpnI G_GTAC'C Cuts at: 2803 4133 2803 Size: 1330 5478 MfeI C'AATT_G Cuts at: 4407 4407 Size: 6808 MluI A'CGCG_T Cuts at: 6684 6684 Size: 6808 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2664 3399 5922 6106 1305 Size: 1359 735 2523 184 2007 Fragments arranged by size: 2523 2007 1359 735 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 2030 10 Size: 54 1425 343 177 21 4788 Fragments arranged by size: 4788 1425 343 177 54 21 MslI CAynn'nnrTG Cuts at: 518 1166 4766 5125 5284 518 Size: 648 3600 359 159 2042 Fragments arranged by size: 3600 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2113 2676 2841 2994 3979 4224 4901 6049 Size: 507 563 165 153 985 245 677 1148 Cuts at: 6049 6294 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 985 677 563 507 245 245 165 153 NcoI C'CATG_G Cuts at: 513 1065 2004 2652 513 Size: 552 939 648 4669 Fragments arranged by size: 4669 939 648 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6808 NheI G'CTAG_C Cuts at: 1052 3142 1052 Size: 2090 4718 NotI GC'GGCC_GC Cuts at: 4255 4255 Size: 6808 NspI r_CATG'y Cuts at: 1132 1229 3373 4166 5711 1132 Size: 97 2144 793 1545 2229 Fragments arranged by size: 2229 2144 1545 793 97 PasI CC'CwG_GG Cuts at: 1348 3089 3376 3937 1348 Size: 1741 287 561 4219 Fragments arranged by size: 4219 1741 561 287 PciI A'CATG_T Cuts at: 5707 5707 Size: 6808 PflMI CCAn_nnn'nTGG Cuts at: 1108 2317 3153 3202 1108 Size: 1209 836 49 4714 Fragments arranged by size: 4714 1209 836 49 PfoI T'CCnGG_A Cuts at: 2343 2391 2548 3474 3948 2343 Size: 48 157 926 474 5203 Fragments arranged by size: 5203 926 474 157 48 PmeI GTTT'AAAC Cuts at: 4112 4112 Size: 6808 PmlI CAC'GTG Cuts at: 3879 3879 Size: 6808 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4004 4036 4877 4909 6390 6422 4004 Size: 32 841 32 1481 32 4390 Fragments arranged by size: 4390 1481 841 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2182 2242 3478 2182 Size: 60 1236 5512 Fragments arranged by size: 5512 1236 60 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6808 PsiI TTA'TAA Cuts at: 4378 4378 Size: 6808 PspOMI G'GGCC_C Cuts at: 2648 2648 Size: 6808 PstI C_TGCA'G Cuts at: 830 4160 830 Size: 3330 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6808 PvuII CAG'CTG Cuts at: 2113 2113 Size: 6808 SacI G_AGCT'C Cuts at: 2196 2991 3426 3656 2196 Size: 795 435 230 5348 Fragments arranged by size: 5348 795 435 230 SacII CC_GC'GG Cuts at: 2677 3980 2677 Size: 1303 5505 SalI G'TCGA_C Cuts at: 1936 4150 1936 Size: 2214 4594 SbfI CC_TGCA'GG Cuts at: 4160 4160 Size: 6808 ScaI AGT'ACT Cuts at: 5056 6768 5056 Size: 1712 5096 SfcI C'TryA_G Cuts at: 826 1045 3175 3708 4156 5291 5972 6163 826 Size: 219 2130 533 448 1135 681 191 1471 Fragments arranged by size: 2130 1471 1135 681 533 448 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6808 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6808 SmaI CCC'GGG Cuts at: 1872 4135 1872 Size: 2263 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2618 4912 5813 6075 820 Size: 197 926 675 2294 901 262 1553 Fragments arranged by size: 2294 1553 926 901 675 262 197 SnaBI TAC'GTA Cuts at: 493 4599 493 Size: 4106 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6808 SphI G_CATG'C Cuts at: 1132 4166 1132 Size: 3034 3774 SspI AAT'ATT Cuts at: 5 52 3624 4732 5 Size: 47 3572 1108 2081 Fragments arranged by size: 3572 2081 1108 47 StuI AGG'CCT Cuts at: 1517 3227 1517 Size: 1710 5098 StyI C'CwwG_G Cuts at: 513 1065 2004 2238 2246 2652 3363 3665 4247 Size: 552 939 234 8 406 711 302 582 Cuts at: 4247 513 Size: 3074 Fragments arranged by size: 3074 939 711 582 552 406 302 234 8 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2177 3601 4835 4852 5005 5190 5635 Size: 1255 597 1424 1234 17 153 185 445 Cuts at: 5635 325 Size: 1498 Fragments arranged by size: 1498 1424 1255 1234 597 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2160 5054 6766 Size: 274 80 33 51 1443 183 2894 1712 Cuts at: 6766 96 Size: 138 Fragments arranged by size: 2894 1712 1443 274 183 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4014 4046 6768 6800 4014 Size: 32 2722 32 4022 Fragments arranged by size: 4022 2722 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6808 XbaI T'CTAG_A Cuts at: 4144 4144 Size: 6808 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2318 3457 3482 2318 Size: 1139 25 5644 Fragments arranged by size: 5644 1139 25 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6808 XmaI C'CCGG_G Cuts at: 1870 4133 1870 Size: 2263 4545 XmnI GAAnn'nnTTC Cuts at: 2729 4937 2729 Size: 2208 4600 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4616 276 Size: 53 83 186 936 3082 2468 Fragments arranged by size: 3082 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BbeI BfrBI BplI BsiWI BstZ17I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SanDI SapI SexAI SfiI SfoI SrfI SwaI