(Linear) (Six-base) MAPSORT of: KIEE2059.seq Check: 4965 from: 1 to: 2103 KIEE2059.orf With 182 enzymes: SgfI * July 15, 2010 18:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1202 1726 2103 Size: 1202 524 377 Fragments arranged by size: 1202 524 377 Acc65I G'GTAC_C Cuts at: 0 794 2103 Size: 794 1309 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 727 2103 Size: 727 1376 AflIII A'CryG_T Cuts at: 0 1850 2103 Size: 1850 253 AhdI GACnn_n'nnGTC Cuts at: 0 42 2103 Size: 42 2061 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 274 284 1069 1900 2103 Size: 274 10 785 831 203 Fragments arranged by size: 831 785 274 203 10 AlwNI CAG_nnn'CTG Cuts at: 0 111 180 1476 2004 2103 Size: 111 69 1296 528 99 Fragments arranged by size: 1296 528 111 99 69 ApaI G_GGCC'C Cuts at: 0 647 2103 Size: 647 1456 ApaLI G'TGCA_C Cuts at: 0 469 2103 Size: 469 1634 ApoI r'AATT_y Cuts at: 0 722 2103 Size: 722 1381 AvaI C'yCGr_G Cuts at: 0 1039 1563 2103 Size: 1039 524 540 Fragments arranged by size: 1039 540 524 AvrII C'CTAG_G Cuts at: 0 1660 2103 Size: 1660 443 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 267 300 2103 Size: 267 33 1803 Fragments arranged by size: 1803 267 33 BamHI G'GATC_C Cuts at: 0 845 2103 Size: 845 1258 BanI G'GyrC_C Cuts at: 0 31 794 2103 Size: 31 763 1309 Fragments arranged by size: 1309 763 31 BanII G_rGCy'C Cuts at: 0 191 647 986 1352 1410 1421 1651 2103 Size: 191 456 339 366 58 11 230 452 Fragments arranged by size: 456 452 366 339 230 191 58 11 BbsI GAAGACnn'nnnn_ Cuts at: 0 1873 2103 Size: 1873 230 BbvCI CC'TCA_GC Cuts at: 0 1217 1240 1903 2103 Size: 1217 23 663 200 Fragments arranged by size: 1217 663 200 23 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 353 387 1168 1202 2103 Size: 353 34 781 34 901 Fragments arranged by size: 901 781 353 34 34 BglII A'GATC_T Cuts at: 0 263 2012 2103 Size: 263 1749 91 Fragments arranged by size: 1749 263 91 BlpI GC'TnA_GC Cuts at: 0 985 1072 2103 Size: 985 87 1031 Fragments arranged by size: 1031 985 87 Bme1580I G_kGCm'C Cuts at: 0 172 273 473 647 2103 Size: 172 101 200 174 1456 Fragments arranged by size: 1456 200 174 172 101 BmgBI CAC'GTC Cuts at: 0 1148 2103 Size: 1148 955 BmrI ACTGGGnnnn_n' Cuts at: 0 149 473 780 1106 2103 Size: 149 324 307 326 997 Fragments arranged by size: 997 326 324 307 149 BmtI G_CTAG'C Cuts at: 0 1141 2103 Size: 1141 962 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 409 943 1219 1546 1855 2103 Size: 409 534 276 327 309 248 Fragments arranged by size: 534 409 327 309 276 248 Bpu10I CC'TnA_GC Cuts at: 0 1217 1240 1650 1682 1903 2103 Size: 1217 23 410 32 221 200 Fragments arranged by size: 1217 410 221 200 32 23 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 598 2103 Size: 598 1505 BsaI GGTCTCn'nnnn_ Cuts at: 0 319 1831 2103 Size: 319 1512 272 Fragments arranged by size: 1512 319 272 BsaAI yAC'GTr Cuts at: 0 759 1874 2103 Size: 759 1115 229 Fragments arranged by size: 1115 759 229 BsaBI GATnn'nnATC Cuts at: 0 1057 2103 Size: 1057 1046 BsaHI Gr'CG_yC Cuts at: 0 812 2103 Size: 812 1291 BsaWI w'CCGG_w Cuts at: 0 492 1590 2103 Size: 492 1098 513 Fragments arranged by size: 1098 513 492 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 418 1267 1324 1978 1992 2103 Size: 418 849 57 654 14 111 Fragments arranged by size: 849 654 418 111 57 14 BseYI C'CCAG_C Cuts at: 0 460 1390 1785 2103 Size: 460 930 395 318 Fragments arranged by size: 930 460 395 318 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 184 190 280 822 1012 1630 2103 Size: 184 6 90 542 190 618 473 Fragments arranged by size: 618 542 473 190 184 90 6 BsiEI CG_ry'CG Cuts at: 0 282 993 2103 Size: 282 711 1110 Fragments arranged by size: 1110 711 282 BsiHKAI G_wGCw'C Cuts at: 0 191 395 473 770 986 1421 1651 1895 Size: 191 204 78 297 216 435 230 244 Cuts at: 1895 2103 Size: 208 Fragments arranged by size: 435 297 244 230 216 208 204 191 78 BsmI GAATG_Cn' Cuts at: 0 1524 2103 Size: 1524 579 BsmBI CGTCTCn'nnnn_ Cuts at: 0 753 2103 Size: 753 1350 Bsp1286I G_dGCh'C Cuts at: 0 172 191 273 395 473 647 770 986 Size: 172 19 82 122 78 174 123 216 Cuts at: 986 1352 1410 1421 1651 1895 2103 Size: 366 58 11 230 244 208 Fragments arranged by size: 366 244 230 216 208 174 172 123 122 82 78 58 19 11 BspEI T'CCGG_A Cuts at: 0 1590 2103 Size: 1590 513 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1202 1256 1484 1726 2103 Size: 1202 54 228 242 377 Fragments arranged by size: 1202 377 242 228 54 BsrBI CCG'CTC Cuts at: 0 216 2103 Size: 216 1887 BsrDI GCAATG_nn' Cuts at: 0 1184 1211 2103 Size: 1184 27 892 Fragments arranged by size: 1184 892 27 BsrFI r'CCGG_y Cuts at: 0 518 2103 Size: 518 1585 BssSI C'ACGA_G Cuts at: 0 1177 2103 Size: 1177 926 BstAPI GCAn_nnn'nTGC Cuts at: 0 2042 2103 Size: 2042 61 BstXI CCAn_nnnn'nTGG Cuts at: 0 654 1084 2103 Size: 654 430 1019 Fragments arranged by size: 1019 654 430 BstYI r'GATC_y Cuts at: 0 263 845 2012 2103 Size: 263 582 1167 91 Fragments arranged by size: 1167 582 263 91 Bsu36I CC'TnA_GG Cuts at: 0 173 2103 Size: 173 1930 BtgI C'CryG_G Cuts at: 0 647 669 977 1577 1972 2103 Size: 647 22 308 600 395 131 Fragments arranged by size: 647 600 395 308 131 22 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 490 1044 1511 2103 Size: 490 554 467 592 Fragments arranged by size: 592 554 490 467 BtsI GCAGTG_nn' Cuts at: 0 172 1005 2103 Size: 172 833 1098 Fragments arranged by size: 1098 833 172 DraI TTT'AAA Cuts at: 0 1623 2103 Size: 1623 480 DraIII CAC_nnn'GTG Cuts at: 0 1959 2103 Size: 1959 144 EaeI y'GGCC_r Cuts at: 0 2 23 279 761 944 990 1320 2103 Size: 2 21 256 482 183 46 330 783 Fragments arranged by size: 783 482 330 256 183 46 21 2 EagI C'GGCC_G Cuts at: 0 279 990 2103 Size: 279 711 1113 Fragments arranged by size: 1113 711 279 EarI CTCTTCn'nnn_ Cuts at: 0 694 2103 Size: 694 1409 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 190 625 820 1984 2017 2103 Size: 190 435 195 1164 33 86 Fragments arranged by size: 1164 435 195 190 86 33 EcoICRI GAG'CTC Cuts at: 0 189 984 1419 1649 2103 Size: 189 795 435 230 454 Fragments arranged by size: 795 454 435 230 189 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 409 727 943 1219 1546 1855 2103 Size: 409 318 216 276 327 309 248 Fragments arranged by size: 409 327 318 309 276 248 216 EcoNI CCTnn'n_nnAGG Cuts at: 0 895 1239 1339 2103 Size: 895 344 100 764 Fragments arranged by size: 895 764 344 100 EcoO109I rG'GnC_Cy Cuts at: 0 177 237 643 1473 1968 2103 Size: 177 60 406 830 495 135 Fragments arranged by size: 830 495 406 177 135 60 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 441 2103 Size: 441 1662 HaeII r_GCGC'y Cuts at: 0 2004 2103 Size: 2004 99 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 28 49 60 81 1286 1318 2103 Size: 28 21 11 21 1205 32 785 Fragments arranged by size: 1205 785 32 28 21 21 11 HincII GTy'rAC Cuts at: 0 816 1743 2103 Size: 816 927 360 Fragments arranged by size: 927 816 360 KpnI G_GTAC'C Cuts at: 0 798 2103 Size: 798 1305 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 659 1394 2103 Size: 659 735 709 Fragments arranged by size: 735 709 659 MscI TGG'CCA Cuts at: 0 4 25 2103 Size: 4 21 2078 Fragments arranged by size: 2078 21 4 MspA1I CmG'CkG Cuts at: 0 108 671 836 989 1974 2103 Size: 108 563 165 153 985 129 Fragments arranged by size: 985 563 165 153 129 108 NcoI C'CATG_G Cuts at: 0 647 2103 Size: 647 1456 NheI G'CTAG_C Cuts at: 0 1137 2103 Size: 1137 966 NspI r_CATG'y Cuts at: 0 1368 2103 Size: 1368 735 PasI CC'CwG_GG Cuts at: 0 1084 1371 1932 2103 Size: 1084 287 561 171 Fragments arranged by size: 1084 561 287 171 PflMI CCAn_nnn'nTGG Cuts at: 0 312 1148 1197 2103 Size: 312 836 49 906 Fragments arranged by size: 906 836 312 49 PfoI T'CCnGG_A Cuts at: 0 338 386 543 1469 1943 2103 Size: 338 48 157 926 474 160 Fragments arranged by size: 926 474 338 160 157 48 PmlI CAC'GTG Cuts at: 0 1874 2103 Size: 1874 229 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1999 2031 2103 Size: 1999 32 72 Fragments arranged by size: 1999 72 32 PpuMI rG'GwC_Cy Cuts at: 0 177 237 1473 2103 Size: 177 60 1236 630 Fragments arranged by size: 1236 630 177 60 PspOMI G'GGCC_C Cuts at: 0 643 2103 Size: 643 1460 PvuII CAG'CTG Cuts at: 0 108 2103 Size: 108 1995 SacI G_AGCT'C Cuts at: 0 191 986 1421 1651 2103 Size: 191 795 435 230 452 Fragments arranged by size: 795 452 435 230 191 SacII CC_GC'GG Cuts at: 0 672 1975 2103 Size: 672 1303 128 Fragments arranged by size: 1303 672 128 SfcI C'TryA_G Cuts at: 0 1170 1703 2103 Size: 1170 533 400 Fragments arranged by size: 1170 533 400 SmlI C'TyrA_G Cuts at: 0 613 2103 Size: 613 1490 SspI AAT'ATT Cuts at: 0 1619 2103 Size: 1619 484 StuI AGG'CCT Cuts at: 0 1222 2103 Size: 1222 881 StyI C'CwwG_G Cuts at: 0 233 241 647 1358 1660 2103 Size: 233 8 406 711 302 443 Fragments arranged by size: 711 443 406 302 233 8 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 172 1596 2103 Size: 172 1424 507 Fragments arranged by size: 1424 507 172 TatI w'GTAC_w Cuts at: 0 155 2103 Size: 155 1948 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2009 2041 2103 Size: 2009 32 62 Fragments arranged by size: 2009 62 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 313 1452 1477 2103 Size: 313 1139 25 626 Fragments arranged by size: 1139 626 313 25 XmnI GAAnn'nnTTC Cuts at: 0 724 2103 Size: 724 1379 Enzymes that do cut: AarI Acc65I AcuI AflIII AhdI AlfI AlwNI ApaI ApaLI ApoI AvaI AvrII BaeI BamHI BanI BanII BbsI BbvCI BcgI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HincII KpnI MmeI MscI MspA1I NcoI NheI NspI PasI PflMI PfoI PmlI PpiI PpuMI PspOMI PvuII SacI SacII SfcI SmlI SspI StuI StyI TaqII TatI TstI XcmI XmnI Enzymes that do not cut: AatII AccI AclI AfeI AflII AgeI AleI AloI AscI AseI AsiSI BbeI BciVI BclI BfrBI BglI BplI BsaXI BsiWI BspHI BsrGI BssHII BstBI BstEII BstZ17I ClaI CspCI DrdI EcoRI EcoRV FseI FspI FspAI HindIII HpaI KasI MfeI MluI MslI NaeI NarI NdeI NgoMIV NotI NruI NsiI PacI PciI PmeI PshAI PsiI PspXI PsrI PstI PvuI RsrII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SwaI Tth111I XbaI XhoI XmaI ZraI