################################## # NO CUT ENZYMES ( 89 enzymes) # ################################## AatII AccI AclI AflII AgeI Aor51HI AscI AseI AsnI AspI AviII BbeI BciVI BclI BfrI BglI BsiWI Bsp1407I BspDI BspHI BspLU11I BsrGI BssHII Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DrdI Eco47III EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII HpaI KasI MfeI MluI MslI MunI NaeI NarI NdeI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SwaI TaqI Tth111I TthHB8I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### Acc65I 794 GGTACC G^GTACC AccIII 1590 TCCGGA T^CCGGA AcsI 722 AAATTT [GA]^AATT[CT] AcyI 811 GGCGTC G[GA]^CG[CT]C AflIII 1850 ACACGT A^C[GA][CT]GT AhdI 37 GACCTCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 469 GTGCAC G^TGCAC AocI 172 CCTCAGG CC^T[ACGT]AGG ApaI 643 GGGCCC GGGCC^C ApaLI 469 GTGCAC G^TGCAC ApoI 722 AAATTT [GA]^AATT[CT] Asp700I 720 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 794 GGTACC G^GTACC AspEI 37 GACCTCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvrII 1660 CCTAGG C^CTAGG BamHI 845 GGATCC G^GATCC BbrPI 1872 CACGTG CAC^GTG BbsI 1866 GAAGAC GAAGAC(2/6) BlnI 1660 CCTAGG C^CTAGG BpuAI 1866 GAAGAC GAAGAC(2/6) BsaBI 1053 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 811 GGCGTC G[GA]^CG[CT]C BseAI 1590 TCCGGA T^CCGGA BsmBI 747 CGTCTC CGTCTC(1/5) BsmI 1518 GAATGC GAATGC(1/-1) BspEI 1590 TCCGGA T^CCGGA BsrBI 214 GAGCGG CCGCTC(-3/-3) BsrFI 518 ACCGGC [GA]^CCGG[CT] BssSI 1177 CACGAG CACGAG(-5/-1) BstAPI 2036 GCAACAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 172 CCTCAGG CC^T[ACGT]AGG Cfr10I 518 ACCGGC [GA]^CCGG[CT] DraI 1621 TTTAAA TTT^AAA DraIII 1954 CACTTCGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 37 GACCTCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 699 GAAGAG CTCTTC(1/4) Eco81I 172 CCTCAGG CC^T[ACGT]AGG HaeII 2000 AGCGCC [GA]GCGC^[CT] Hin1I 811 GGCGTC G[GA]^CG[CT]C KpnI 794 GGTACC GGTAC^C Ksp632I 699 GAAGAG CTCTTC(1/4) MamI 1053 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 1590 TCCGGA T^CCGGA NcoI 647 CCATGG C^CATGG NheI 1137 GCTAGC G^CTAGC NspI 1364 GCATGT [GA]CATG^[CT] PmaCI 1872 CACGTG CAC^GTG PmlI 1872 CACGTG CAC^GTG PvuII 106 CAGCTG CAG^CTG SmlI 613 CTCAAG C^T[CT][GA]AG SspI 1617 AATATT AAT^ATT StuI 1220 AGGCCT AGG^CCT XmnI 720 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AvaI 1039 CCCGAG C^[CT]CG[GA]G 1563 CCCGAG BalI 2 TGGCCA TGG^CCA 23 TGGCCA BanI 31 GGCACC G^G[CT][GA]CC 794 GGTACC BcgI 1179 CGAGGCCATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 364 GCAGATGACTCG BfaI 1138 CTAG C^TAG 1661 CTAG BglII 263 AGATCT A^GATCT 2012 AGATCT BlpI 984 GCTCAGC GC^T[ACGT]AGC 1071 GCTCAGC Bpu1102I 984 GCTCAGC GC^T[ACGT]AGC 1071 GCTCAGC BsaAI 757 TACGTG [CT]AC^GT[GA] 1872 CACGTG BsaI 325 GAGACC GGTCTC(1/5) 1837 GAGACC BsaWI 492 TCCGGT [AT]^CCGG[AT] 1590 TCCGGA BsiEI 279 CGGCCG CG[GA][CT]^CG 990 CGGCCG BsoBI 1039 CCCGAG C^[CT]CG[GA]G 1563 CCCGAG BsrDI 1185 CATTGC GCAATG(2/0) 1212 CATTGC BstXI 647 CCATGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1077 CCAGACCCCTGG CelII 984 GCTCAGC GC^T[ACGT]AGC 1071 GCTCAGC EagI 279 CGGCCG C^GGCCG 990 CGGCCG EclXI 279 CGGCCG C^GGCCG 990 CGGCCG Eco52I 279 CGGCCG C^GGCCG 990 CGGCCG HincII 814 GTCAAC GT[CT]^[GA]AC 1741 GTCAAC HindII 814 GTCAAC GT[CT]^[GA]AC 1741 GTCAAC KspI 669 CCGCGG CCGC^GG 1972 CCGCGG MaeI 1138 CTAG C^TAG 1661 CTAG MluNI 2 TGGCCA TGG^CCA 23 TGGCCA MscI 2 TGGCCA TGG^CCA 23 TGGCCA SacII 669 CCGCGG CCGC^GG 1972 CCGCGG SfcI 1170 CTACAG C^T[GA][CT]AG 1703 CTATAG ######################################### # 3 OR MORE CUT ENZYMES ( 89 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AlwNI AspHI AvaII BanII BbvCI BbvI BcnI BmyI BpmI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PflMI PleI PpuMI RsaI SacI Sau3AI Sau96I ScrFI SfaNI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Van91I XcmI XhoII