ALOX12 ORF clone pFN21AE2045

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Halotag ORF Clone pFN21AE2045

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Clone info.

Species

human

Clone Name

pFN21AE2045 Sequence (with vector) Restriction Map

Product ID

FHC25861

ORF Sequence

DNA sequence (1989 bp)
Protein sequence (663 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB590844]

Gene

KIEE2045

Symbol

ALOX12

Alias : 12-LOX, 12S-LOX, LOG12

Description

arachidonate 12-lipoxygenase

Original Clone ID

cs02945

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1989 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 663 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS11084.1 0 100.0 ALOX12 CCDS11049.1 4.9e-184 65.5 ALOX15 CCDS7212.1 5.1e-102 42.0 ALOX5 CCDS11128.1 9.6e-89 38.0 ALOX15B CCDS58078.1 2.8e-87 42.4 ALOX5 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR001885 11 28 PR00467 Lipoxygenase IPR001885 57 76 PR00467 Lipoxygenase IPR001885 133 147 PR00467 Lipoxygenase IPR001885 197 218 PR00467 Lipoxygenase IPR001885 294 313 PR00467 Lipoxygenase IPR013819 337 354 PR00087 Lipoxygenase IPR013819 355 372 PR00087 Lipoxygenase IPR013819 375 395 PR00087 Lipoxygenase IPR001885 420 442 PR00467 Lipoxygenase IPR001885 580 597 PR00467 Lipoxygenase HMMPfam IPR001024 3 107 PF01477 Lipoxygenase IPR013819 147 655 PF00305 Lipoxygenase HMMSmart IPR001024 2 111 SM00308 Lipoxygenase ProfileScan IPR001024 2 114 PS50095 Lipoxygenase IPR013819 114 663 PS51393 Lipoxygenase
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_000688 663 152391 100.0 100.0 NP_001131 662 152392 65.5 100.0 XP_011522082 713 152391 89.6 100.0 NP_000689 674 152390,600807 42.0 100.0 NP_001132 676 603697 38.0 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE2045 Download

>KIEE2045  1989 bp
ATGGGCCGCTACCGCATCCGCGTGGCCACCGGGGCCTGGCTCTTCTCCGGGTCGTACAAC
CGCGTGCAGCTTTGGCTGGTCGGGACGCGCGGGGAGGCGGAGCTGGAGCTGCAGCTGCGG
CCGGCGCGGGGCGAGGAGGAGGAGTTTGATCATGACGTTGCAGAGGACTTGGGGCTCCTG
CAGTTCGTGAGGCTGCGCAAGCACCACTGGCTGGTGGACGACGCGTGGTTCTGCGACCGC
ATCACGGTGCAGGGCCCTGGAGCCTGCGCGGAGGTGGCCTTCCCGTGCTACCGCTGGGTG
CAGGGCGAGGACATCCTGAGCCTGCCCGAGGGCACCGCCCGCCTGCCAGGAGACAATGCT
TTGGACATGTTCCAGAAGCATCGAGAGAAGGAACTGAAAGACAGACAGCAGATCTACTGC
TGGGCCACCTGGAAGGAAGGGTTACCCCTGACCATCGCTGCAGACCGTAAGGATGATCTA
CCTCCAAATATGAGATTCCATGAGGAGAAGAGGCTGGACTTTGAATGGACACTGAAGGCA
GGGGCTCTGGAGATGGCCCTCAAACGTGTTTACACCCTCCTGAGCTCCTGGAACTGCCTA
GAAGACTTTGATCAGATCTTCTGGGGCCAGAAGAGTGCCCTGGCTGAGAAGGTTCGCCAG
TGCTGGCAGGATGATGAGTTGTTCAGCTACCAGTTCCTCAATGGTGCCAACCCCATGCTG
TTGAGACGCTCGACCTCTCTACCCTCCAGGCTAGTGCTGCCCTCGGGGATGGAAGAGCTT
CAGGCTCAACTGGAGAAAGAACTTCAGAATGGTTCCCTGTTTGAAGCTGACTTCATCCTT
CTGGATGGAATTCCAGCCAACGTGATCCGAGGAGAGAAGCAATACCTGGCTGCCCCCCTC
GTTATGCTGAAGATGGAGCCCAATGGGAAGCTGCAGCCCATGGTCATCCAGATTCAGCCT
CCCAACCCCAGCTCTCCAACCCCAACACTGTTCCTGCCCTCAGACCCCCCACTTGCCTGG
CTCCTGGCAAAGTCCTGGGTCCGAAATTCAGATTTCCAACTGCACGAGATCCAGTATCAC
TTGCTGAACACGCACCTGGTGGCTGAGGTCATCGCTGTCGCCACCATGCGGTGCCTCCCA
GGACTGCACCCCATCTTCAAGTTCCTGATCCCCCATATCCGCTACACCATGGAAATCAAC
ACCCGGGCCCGGACCCAACTCATCTCAGATGGAGGAATTTTTGATAAGGCAGTGAGCACA
GGTGGAGGGGGTCATGTACAGTTGCTCCGTCGGGCGGCAGCTCAGCTGACCTACTGCTCC
CTCTGTCCTCCTGACGACCTGGCTGACCGGGGCCTGCTGGGACTCCCAGGTGCTCTCTAT
GCCCATGATGCTTTACGGCTCTGGGAGATCATTGCCAGGTATGTGGAGGGGATCGTCCAC
CTCTTCTACCAAAGGGATGACATAGTGAAGGGGGACCCTGAGCTGCAGGCCTGGTGTCGG
GAGATCACGGAGGTGGGGCTGTGCCAGGCCCAGGACCGAGGTTTCCCTGTCTCCTTCCAG
TCCCAGAGTCAACTCTGCCATTTCCTCACCATGTGCGTCTTCACGTGCACTGCCCAGCAT
GCCGCCATCAACCAGGGCCAGCTGGACTGGTATGCCTGGGTCCCTAATGCTCCATGCACA
ATGCGGATGCCCCCACCCACCACCAAGGAAGATGTGACGATGGCCACAGTGATGGGGTCA
CTACCTGATGTCCGGCAGGCCTGTCTTCAAATGGCCATCTCATGGCATCTGAGTCGCCGC
CAGCCAGACATGGTGCCTCTGGGGCACCACAAAGAAAAATATTTCTCAGGCCCCAAGCCC
AAAGCTGTGCTAAACCAATTCCGAACAGATTTGGAAAAGCTGGAAAAGGAGATTACAGCC
CGGAATGAGCAACTTGACTGGCCCTATGAATATCTGAAGCCCAGCTGCATAGAGAACAGT
GTCACCATC

ORF Protein

Cloned ORF protein sequence for pFN21AE2045 Download

>KIEE2045  663 aa
MGRYRIRVATGAWLFSGSYNRVQLWLVGTRGEAELELQLRPARGEEEEFDHDVAEDLGLL
QFVRLRKHHWLVDDAWFCDRITVQGPGACAEVAFPCYRWVQGEDILSLPEGTARLPGDNA
LDMFQKHREKELKDRQQIYCWATWKEGLPLTIAADRKDDLPPNMRFHEEKRLDFEWTLKA
GALEMALKRVYTLLSSWNCLEDFDQIFWGQKSALAEKVRQCWQDDELFSYQFLNGANPML
LRRSTSLPSRLVLPSGMEELQAQLEKELQNGSLFEADFILLDGIPANVIRGEKQYLAAPL
VMLKMEPNGKLQPMVIQIQPPNPSSPTPTLFLPSDPPLAWLLAKSWVRNSDFQLHEIQYH
LLNTHLVAEVIAVATMRCLPGLHPIFKFLIPHIRYTMEINTRARTQLISDGGIFDKAVST
GGGGHVQLLRRAAAQLTYCSLCPPDDLADRGLLGLPGALYAHDALRLWEIIARYVEGIVH
LFYQRDDIVKGDPELQAWCREITEVGLCQAQDRGFPVSFQSQSQLCHFLTMCVFTCTAQH
AAINQGQLDWYAWVPNAPCTMRMPPPTTKEDVTMATVMGSLPDVRQACLQMAISWHLSRR
QPDMVPLGHHKEKYFSGPKPKAVLNQFRTDLEKLEKEITARNEQLDWPYEYLKPSCIENS
VTI

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE2045 Download

>pFN21AE2045  6694 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGGCCGCTACCGCATCCGCGTGGCCACCGGGGC
CTGGCTCTTCTCCGGGTCGTACAACCGCGTGCAGCTTTGGCTGGTCGGGACGCGCGGGGA
GGCGGAGCTGGAGCTGCAGCTGCGGCCGGCGCGGGGCGAGGAGGAGGAGTTTGATCATGA
CGTTGCAGAGGACTTGGGGCTCCTGCAGTTCGTGAGGCTGCGCAAGCACCACTGGCTGGT
GGACGACGCGTGGTTCTGCGACCGCATCACGGTGCAGGGCCCTGGAGCCTGCGCGGAGGT
GGCCTTCCCGTGCTACCGCTGGGTGCAGGGCGAGGACATCCTGAGCCTGCCCGAGGGCAC
CGCCCGCCTGCCAGGAGACAATGCTTTGGACATGTTCCAGAAGCATCGAGAGAAGGAACT
GAAAGACAGACAGCAGATCTACTGCTGGGCCACCTGGAAGGAAGGGTTACCCCTGACCAT
CGCTGCAGACCGTAAGGATGATCTACCTCCAAATATGAGATTCCATGAGGAGAAGAGGCT
GGACTTTGAATGGACACTGAAGGCAGGGGCTCTGGAGATGGCCCTCAAACGTGTTTACAC
CCTCCTGAGCTCCTGGAACTGCCTAGAAGACTTTGATCAGATCTTCTGGGGCCAGAAGAG
TGCCCTGGCTGAGAAGGTTCGCCAGTGCTGGCAGGATGATGAGTTGTTCAGCTACCAGTT
CCTCAATGGTGCCAACCCCATGCTGTTGAGACGCTCGACCTCTCTACCCTCCAGGCTAGT
GCTGCCCTCGGGGATGGAAGAGCTTCAGGCTCAACTGGAGAAAGAACTTCAGAATGGTTC
CCTGTTTGAAGCTGACTTCATCCTTCTGGATGGAATTCCAGCCAACGTGATCCGAGGAGA
GAAGCAATACCTGGCTGCCCCCCTCGTTATGCTGAAGATGGAGCCCAATGGGAAGCTGCA
GCCCATGGTCATCCAGATTCAGCCTCCCAACCCCAGCTCTCCAACCCCAACACTGTTCCT
GCCCTCAGACCCCCCACTTGCCTGGCTCCTGGCAAAGTCCTGGGTCCGAAATTCAGATTT
CCAACTGCACGAGATCCAGTATCACTTGCTGAACACGCACCTGGTGGCTGAGGTCATCGC
TGTCGCCACCATGCGGTGCCTCCCAGGACTGCACCCCATCTTCAAGTTCCTGATCCCCCA
TATCCGCTACACCATGGAAATCAACACCCGGGCCCGGACCCAACTCATCTCAGATGGAGG
AATTTTTGATAAGGCAGTGAGCACAGGTGGAGGGGGTCATGTACAGTTGCTCCGTCGGGC
GGCAGCTCAGCTGACCTACTGCTCCCTCTGTCCTCCTGACGACCTGGCTGACCGGGGCCT
GCTGGGACTCCCAGGTGCTCTCTATGCCCATGATGCTTTACGGCTCTGGGAGATCATTGC
CAGGTATGTGGAGGGGATCGTCCACCTCTTCTACCAAAGGGATGACATAGTGAAGGGGGA
CCCTGAGCTGCAGGCCTGGTGTCGGGAGATCACGGAGGTGGGGCTGTGCCAGGCCCAGGA
CCGAGGTTTCCCTGTCTCCTTCCAGTCCCAGAGTCAACTCTGCCATTTCCTCACCATGTG
CGTCTTCACGTGCACTGCCCAGCATGCCGCCATCAACCAGGGCCAGCTGGACTGGTATGC
CTGGGTCCCTAATGCTCCATGCACAATGCGGATGCCCCCACCCACCACCAAGGAAGATGT
GACGATGGCCACAGTGATGGGGTCACTACCTGATGTCCGGCAGGCCTGTCTTCAAATGGC
CATCTCATGGCATCTGAGTCGCCGCCAGCCAGACATGGTGCCTCTGGGGCACCACAAAGA
AAAATATTTCTCAGGCCCCAAGCCCAAAGCTGTGCTAAACCAATTCCGAACAGATTTGGA
AAAGCTGGAAAAGGAGATTACAGCCCGGAATGAGCAACTTGACTGGCCCTATGAATATCT
GAAGCCCAGCTGCATAGAGAACAGTGTCACCATCGTTTAAACGAATTCGGGCTCGGTACC
CGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCC
GAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGG
CGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGA
ATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACC
ATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTT
CAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATC
GAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTG
ACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAG
ACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAA
ATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATAT
TGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCG
GCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAA
GATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTT
GAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGT
GGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTAT
TCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATG
ACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTA
CTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGAT
CATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAG
CGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAA
CTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCA
GGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCC
GGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGT
ATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATC
GCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACG
CCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATA
ACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCG
CGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCT
CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAA
GCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTC
TCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGT
AGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCG
CCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGG
CAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCT
TGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGC
TGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCG
CTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTC
AAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTT
AAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCG
GAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGG
CAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTC
ATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTT
TTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGA
TTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCG
GCCACGATGCGTCCGGCGTAGAGGATCGAGATCT