(Circular) (Six-base) MAPSORT of: pFN21AE2045.seq Check: 2315 from: 1 to: 6694 pFN21AE2045 6694 bp With 182 enzymes: SgfI * August 16, 2010 14:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4504 278 Size: 53 83 186 936 2968 2468 Fragments arranged by size: 2968 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4037 1937 Size: 2100 4594 Acc65I G'GTAC_C Cuts at: 4015 4015 Size: 6694 AclI AA'CG_TT Cuts at: 1563 4821 5194 1563 Size: 3258 373 3063 Fragments arranged by size: 3258 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2558 2768 2792 2933 3980 4757 6141 6409 Size: 926 210 24 141 1047 777 1384 268 Cuts at: 6409 1632 Size: 1917 Fragments arranged by size: 1917 1384 1047 926 777 268 210 141 24 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6497 AflIII A'CryG_T Cuts at: 1203 2226 2370 2569 5593 6570 1203 Size: 1023 144 199 3024 977 1327 Fragments arranged by size: 3024 1327 1023 977 199 144 AgeI A'CCGG_T Cuts at: 5525 5525 Size: 6694 AhdI GACnn_n'nnGTC Cuts at: 5423 5423 Size: 6694 AleI CACnn'nnGTG Cuts at: 1166 3515 1166 Size: 2349 4345 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2267 1184 Size: 1083 5611 AlwNI CAG_nnn'CTG Cuts at: 4437 6009 4437 Size: 1572 5122 ApaI G_GGCC'C Cuts at: 2261 3214 2261 Size: 953 5741 ApaLI G'TGCA_C Cuts at: 3610 4751 5907 3610 Size: 1141 1156 4397 Fragments arranged by size: 4397 1156 1141 ApoI r'AATT_y Cuts at: 1493 2853 3049 3240 4003 4229 4381 6490 1493 Size: 1360 196 191 763 226 152 2109 1697 Fragments arranged by size: 2109 1697 1360 763 226 196 191 152 AseI AT'TA_AT Cuts at: 160 1032 5248 160 Size: 872 4216 1606 Fragments arranged by size: 4216 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6694 AvaI C'yCGr_G Cuts at: 1870 1943 2330 2767 3207 4019 1870 Size: 73 387 437 440 812 4545 Fragments arranged by size: 4545 812 440 437 387 73 BamHI G'GATC_C Cuts at: 4024 4391 4024 Size: 367 6327 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2336 2708 3135 3817 3828 Size: 325 217 627 549 372 427 682 11 Cuts at: 3828 4015 5470 618 Size: 187 1455 1842 Fragments arranged by size: 1842 1455 682 627 549 427 372 325 217 187 11 BanII G_rGCy'C Cuts at: 1391 2181 2261 2551 2591 2925 3214 4013 1391 Size: 790 80 290 40 334 289 799 4072 Fragments arranged by size: 4072 799 790 334 290 289 80 40 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2613 3595 3761 928 Size: 971 714 982 166 3861 Fragments arranged by size: 3861 982 971 714 166 BbvCI CC'TCA_GC Cuts at: 3108 3108 Size: 6694 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3779 3813 4885 4919 1903 Size: 34 1842 34 1072 34 3678 Fragments arranged by size: 3678 1842 1072 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4586 5796 4586 Size: 1210 5484 BclI T'GATC_A Cuts at: 1548 2152 2614 1548 Size: 604 462 5628 Fragments arranged by size: 5628 604 462 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2036 3826 5305 Size: 107 122 71 631 605 364 1790 1479 Cuts at: 5305 136 Size: 1525 Fragments arranged by size: 1790 1525 1479 631 605 364 122 107 71 BglII A'GATC_T Cuts at: 2415 2619 6689 2415 Size: 204 4070 2420 Fragments arranged by size: 4070 2420 204 BlpI GC'TnA_GC Cuts at: 3306 4111 3306 Size: 805 5889 Bme1580I G_kGCm'C Cuts at: 1790 2261 2339 2644 3214 3614 3831 4755 5911 Size: 471 78 305 570 400 217 924 1156 Cuts at: 5911 1790 Size: 2573 Fragments arranged by size: 2573 1156 924 570 471 400 305 217 78 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6694 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4425 5383 442 Size: 416 53 83 399 24 3008 958 1753 Fragments arranged by size: 3008 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6694 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2129 2283 2573 2734 2816 5354 1239 Size: 890 154 290 161 82 2538 2579 Fragments arranged by size: 2579 2538 890 290 161 154 82 Bpu10I CC'TnA_GC Cuts at: 2321 2585 3108 3483 2321 Size: 264 523 375 5532 Fragments arranged by size: 5532 523 375 264 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4819 5684 5982 4819 Size: 865 298 5531 Fragments arranged by size: 5531 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5357 882 Size: 617 3858 2219 Fragments arranged by size: 3858 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3609 4485 493 Size: 711 2405 876 2702 Fragments arranged by size: 2702 2405 876 711 BsaBI GATnn'nnATC Cuts at: 6688 6688 Size: 6694 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4501 4883 5518 275 Size: 53 83 186 936 2968 382 635 1451 Fragments arranged by size: 2968 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5127 5525 5799 5946 6357 5127 Size: 398 274 147 411 5464 Fragments arranged by size: 5464 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2132 2162 3273 3303 1178 Size: 30 165 30 729 30 1111 30 4569 Fragments arranged by size: 4569 1111 729 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2153 2156 2159 2522 2889 1186 Size: 967 3 3 363 367 4991 Fragments arranged by size: 4991 967 367 363 3 3 BseYI C'CCAG_C Cuts at: 2298 2424 2972 3361 3618 3965 5897 6393 2298 Size: 126 548 389 257 347 1932 496 2599 Fragments arranged by size: 2599 1932 548 496 389 347 257 126 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2090 2273 2324 3050 3134 1163 Size: 573 354 183 51 726 84 4723 Fragments arranged by size: 4723 726 573 354 183 84 51 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2126 2242 4144 4905 5933 1540 Size: 275 186 125 116 1902 761 1028 2301 Fragments arranged by size: 2301 1902 1028 761 275 186 125 116 BsiHKAI G_wGCw'C Cuts at: 2591 3263 3379 3614 4755 4840 5911 2591 Size: 672 116 235 1141 85 1071 3374 Fragments arranged by size: 3374 1141 1071 672 235 116 85 BsmI GAATG_Cn' Cuts at: 4205 4298 4205 Size: 93 6601 BsmBI CGTCTCn'nnnn_ Cuts at: 2722 2722 Size: 6694 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2181 2261 2339 2551 2591 2644 2925 Size: 399 391 80 78 212 40 53 281 Cuts at: 2925 3214 3263 3379 3614 3831 4013 4755 4840 Size: 289 49 116 235 217 182 742 85 Cuts at: 4840 5911 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 391 289 281 235 217 212 182 116 85 80 78 53 49 40 BspEI T'CCGG_A Cuts at: 6357 6357 Size: 6694 BspHI T'CATG_A Cuts at: 2155 4583 6313 2155 Size: 2428 1730 2536 Fragments arranged by size: 2536 2428 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4049 844 Size: 3205 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4581 1394 Size: 429 2758 3507 Fragments arranged by size: 3507 2758 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3414 5189 5363 65 Size: 1183 2166 1775 174 1396 Fragments arranged by size: 2166 1775 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2125 5338 5525 1700 Size: 110 315 3213 187 2869 Fragments arranged by size: 3213 2869 315 187 110 BsrGI T'GTAC_A Cuts at: 96 3280 96 Size: 3184 3510 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6694 BssSI C'ACGA_G Cuts at: 3068 4754 5766 3068 Size: 1686 1012 3996 Fragments arranged by size: 3996 1686 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6694 BstBI TT'CG_AA Cuts at: 5498 5498 Size: 6694 BstEII G'GTnAC_C Cuts at: 1184 2445 1184 Size: 1261 5433 BstXI CCAn_nnnn'nTGG Cuts at: 3021 3427 3736 3021 Size: 406 309 5979 Fragments arranged by size: 5979 406 309 BstYI r'GATC_y Cuts at: 1972 2415 2619 3072 4024 4391 4776 4793 6245 Size: 443 204 453 952 367 385 17 1452 Cuts at: 6245 6331 6343 6689 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 952 453 443 385 367 346 204 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2943 3192 513 Size: 552 939 939 249 4015 Fragments arranged by size: 4015 939 939 552 249 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2443 3100 487 Size: 683 56 1217 657 4081 Fragments arranged by size: 4081 1217 683 657 56 BtsI GCAGTG_nn' Cuts at: 3261 3612 4210 5004 5024 3261 Size: 351 598 794 20 4931 Fragments arranged by size: 4931 794 598 351 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6694 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6659 DraI TTT'AAA Cuts at: 3998 3998 Size: 6694 DraIII CAC_nnn'GTG Cuts at: 3103 3597 3103 Size: 494 6200 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5701 809 Size: 507 4385 1802 Fragments arranged by size: 4385 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2028 2123 3726 Size: 54 1425 50 275 18 198 95 1603 Cuts at: 3726 3777 4141 5030 6659 8 Size: 51 364 889 1629 43 Fragments arranged by size: 1629 1603 1425 889 364 275 198 95 54 51 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2123 4141 1537 Size: 275 311 2018 4090 Fragments arranged by size: 4090 2018 311 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2051 2507 2630 2772 3452 4624 1353 Size: 698 456 123 142 680 1172 3423 Fragments arranged by size: 3423 1172 698 680 456 142 123 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2117 5281 5655 5801 387 Size: 1064 138 528 3164 374 146 1280 Fragments arranged by size: 3164 1280 1064 528 374 146 138 EcoICRI GAG'CTC Cuts at: 2589 2589 Size: 6694 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2129 2283 2558 2573 2734 2768 2792 Size: 393 497 154 275 15 161 34 24 Cuts at: 2792 2816 2933 3980 4757 5354 6141 6409 1239 Size: 24 117 1047 777 597 787 268 1524 Fragments arranged by size: 1524 1047 787 777 597 497 393 275 268 161 154 117 34 24 24 15 EcoNI CCTnn'n_nnAGG Cuts at: 2330 2330 Size: 6694 EcoO109I rG'GnC_Cy Cuts at: 2037 2257 2258 3355 3478 3664 3854 2037 Size: 220 1 1097 123 186 190 4877 Fragments arranged by size: 4877 1097 220 190 186 123 1 EcoRI G'AATT_C Cuts at: 2853 4003 4381 2853 Size: 1150 378 5166 Fragments arranged by size: 5166 1150 378 FspI TGC'GCA Cuts at: 2201 5200 2201 Size: 2999 3695 HaeII r_GCGC'y Cuts at: 1678 5841 1678 Size: 4163 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2400 2432 2947 2979 3064 Size: 32 135 32 828 32 515 32 85 Cuts at: 3064 3096 5335 5367 5409 5441 1373 Size: 32 2239 32 42 32 2626 Fragments arranged by size: 2626 2239 828 515 135 85 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3575 4038 4284 669 Size: 988 281 1637 463 246 3079 Fragments arranged by size: 3079 1637 988 463 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6694 HpaI GTT'AAC Cuts at: 4284 4284 Size: 6694 KpnI G_GTAC'C Cuts at: 4019 4019 Size: 6694 MfeI C'AATT_G Cuts at: 4293 4293 Size: 6694 MluI A'CGCG_T Cuts at: 2226 6570 2226 Size: 4344 2350 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3005 3085 5808 5992 1305 Size: 1700 80 2723 184 2007 Fragments arranged by size: 2723 2007 1700 184 80 MscI TGG'CCA Cuts at: 10 64 1489 1832 2030 3728 3779 10 Size: 54 1425 343 198 1698 51 2925 Fragments arranged by size: 2925 1698 1425 343 198 54 51 MslI CAynn'nnrTG Cuts at: 518 1166 2024 2249 3515 3734 4652 5011 5170 Size: 648 858 225 1266 219 918 359 159 Cuts at: 5170 518 Size: 2042 Fragments arranged by size: 2042 1266 918 858 648 359 225 219 159 MspA1I CmG'CkG Cuts at: 1606 2119 2298 3310 3646 3969 4110 4787 5935 Size: 513 179 1012 336 323 141 677 1148 Cuts at: 5935 6180 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 1012 677 513 336 323 245 179 141 NaeI GCC'GGC Cuts at: 2127 2127 Size: 6694 NcoI C'CATG_G Cuts at: 513 1065 2004 2943 3192 513 Size: 552 939 939 249 4015 Fragments arranged by size: 4015 939 939 552 249 NdeI CA'TA_TG Cuts at: 387 387 Size: 6694 NgoMIV G'CCGG_C Cuts at: 2125 2125 Size: 6694 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6694 NotI GC'GGCC_GC Cuts at: 4141 4141 Size: 6694 NspI r_CATG'y Cuts at: 1132 1229 2374 3626 4052 5597 1132 Size: 97 1145 1252 426 1545 2229 Fragments arranged by size: 2229 1545 1252 1145 426 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6694 PciI A'CATG_T Cuts at: 2370 5593 2370 Size: 3223 3471 PflMI CCAn_nnn'nTGG Cuts at: 1108 2215 3786 1108 Size: 1107 1571 4016 Fragments arranged by size: 4016 1571 1107 PfoI T'CCnGG_A Cuts at: 2591 2591 Size: 6694 PmeI GTTT'AAAC Cuts at: 3998 3998 Size: 6694 PmlI CAC'GTG Cuts at: 3609 3609 Size: 6694 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4763 4795 6276 6308 4763 Size: 32 1481 32 5149 Fragments arranged by size: 5149 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3478 3664 3478 Size: 186 6508 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6694 PsiI TTA'TAA Cuts at: 4264 4264 Size: 6694 PspOMI G'GGCC_C Cuts at: 2257 3210 2257 Size: 953 5741 PstI C_TGCA'G Cuts at: 830 2118 2187 2467 2940 3492 4046 830 Size: 1288 69 280 473 552 554 3478 Fragments arranged by size: 3478 1288 554 552 473 280 69 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6694 PvuII CAG'CTG Cuts at: 2119 3310 3646 3969 2119 Size: 1191 336 323 4844 Fragments arranged by size: 4844 1191 336 323 SacI G_AGCT'C Cuts at: 2591 2591 Size: 6694 SalI G'TCGA_C Cuts at: 1936 4036 1936 Size: 2100 4594 SanDI GG'GwC_CC Cuts at: 3478 3664 3478 Size: 186 6508 SapI GCTCTTCn'nnn_ Cuts at: 2051 2772 2051 Size: 721 5973 SbfI CC_TGCA'GG Cuts at: 4046 4046 Size: 6694 ScaI AGT'ACT Cuts at: 4942 6654 4942 Size: 1712 4982 SexAI A'CCwGG_T Cuts at: 3099 3099 Size: 6694 SfcI C'TryA_G Cuts at: 826 1045 2114 2183 2463 2936 3488 4042 5177 Size: 219 1069 69 280 473 552 554 1135 Cuts at: 5177 5858 6049 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1069 681 554 552 473 280 219 191 69 SfiI GGCCn_nnn'nGGCC Cuts at: 2036 2036 Size: 6694 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6694 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6694 SmaI CCC'GGG Cuts at: 1872 3209 4021 1872 Size: 1337 812 4545 Fragments arranged by size: 4545 1337 812 SmlI C'TyrA_G Cuts at: 820 1017 1943 4798 5699 5961 820 Size: 197 926 2855 901 262 1553 Fragments arranged by size: 2855 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4485 493 Size: 3992 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6694 SphI G_CATG'C Cuts at: 1132 3626 4052 1132 Size: 2494 426 3774 Fragments arranged by size: 3774 2494 426 SspI AAT'ATT Cuts at: 5 52 3845 4618 5 Size: 47 3793 773 2081 Fragments arranged by size: 3793 2081 773 47 StuI AGG'CCT Cuts at: 1517 3494 3764 1517 Size: 1977 270 4447 Fragments arranged by size: 4447 1977 270 StyI C'CwwG_G Cuts at: 513 1065 2004 2943 3192 3708 4133 513 Size: 552 939 939 249 516 425 3074 Fragments arranged by size: 3074 939 939 552 516 425 249 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2290 3555 3715 4721 4738 4891 5076 Size: 1255 710 1265 160 1006 17 153 185 Cuts at: 5076 5521 325 Size: 445 1498 Fragments arranged by size: 1498 1265 1255 1006 710 445 185 160 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3280 4940 6652 Size: 274 80 33 51 1443 1303 1660 1712 Cuts at: 6652 96 Size: 138 Fragments arranged by size: 1712 1660 1443 1303 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2213 2245 6654 6686 2213 Size: 32 4409 32 2221 Fragments arranged by size: 4409 2221 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6694 XbaI T'CTAG_A Cuts at: 4030 4030 Size: 6694 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2702 2702 Size: 6694 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6694 XmaI C'CCGG_G Cuts at: 1870 3207 4019 1870 Size: 1337 812 4545 Fragments arranged by size: 4545 1337 812 XmnI GAAnn'nnTTC Cuts at: 2781 4823 2781 Size: 2042 4652 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4502 276 Size: 53 83 186 936 2968 2468 Fragments arranged by size: 2968 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I Bsu36I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PspXI PsrI RsrII SacII SfoI SrfI SwaI