################################## # NO CUT ENZYMES ( 96 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AgeI AhdI AlwNI AocI Aor51HI AscI AseI AsnI Asp718I AspEI AspI AvrII BamHI BbeI BciVI BfrI BlnI BsaBI BsaHI BsaI BsaWI BseAI BsiWI BsmI BspDI BspEI BspMI BsrBI BssHII Bst1107I BstAPI BstBI BstZ17I Bsu36I ClaI CpoI DraI DrdI Eam1105I Eco47III Eco81I EcoRV EcoT22I FseI HaeII Hin1I HindIII HpaI KasI KpnI KspI MamI MfeI MroI MseI MunI NarI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SbfI ScaI SfoI SfuI SgrAI SmlI SnaBI SpeI Sse8387I SwaI Tru9I Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### Alw44I 1605 GTGCAC G^TGCAC ApaLI 1605 GTGCAC G^TGCAC Asp700I 772 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AviII 194 TGCGCA TGC^GCA BbrPI 1602 CACGTG CAC^GTG BbvCI 1102 GCTGAGG CCTCAGC(-5/-2) BcgI 1785 GCATCTGAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlpI 1300 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1300 GCTCAGC GC^T[ACGT]AGC BsaAI 1602 CACGTG [CT]AC^GT[GA] BsmBI 723 GAGACG CGTCTC(1/5) Bsp1407I 1275 TGTACA T^GTACA BspHI 150 TCATGA T^CATGA BspLU11I 365 ACATGT A^CATGT BsrDI 1410 CATTGC GCAATG(2/0) BsrFI 120 GCCGGC [GA]^CCGG[CT] BsrGI 1275 TGTACA T^GTACA BssSI 1063 CACGAG CACGAG(-5/-1) BstEII 440 GGTTACC G^GT[ACGT]ACC BstPI 440 GGTTACC G^GT[ACGT]ACC CelII 1300 GCTCAGC GC^T[ACGT]AGC Cfr10I 120 GCCGGC [GA]^CCGG[CT] EagI 118 CGGCCG C^GGCCG EclXI 118 CGGCCG C^GGCCG Eco52I 118 CGGCCG C^GGCCG EcoNI 321 CCTGCCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 440 GGTTACC G^GT[ACGT]ACC EcoRI 848 GAATTC G^AATTC FspI 194 TGCGCA TGC^GCA HincII 1568 GTCAAC GT[CT]^[GA]AC HindII 1568 GTCAAC GT[CT]^[GA]AC MluI 221 ACGCGT A^CGCGT NaeI 120 GCCGGC GCC^GGC NgoMIV 120 GCCGGC G^CCGGC PmaCI 1602 CACGTG CAC^GTG PmlI 1602 CACGTG CAC^GTG RcaI 150 TCATGA T^CATGA SacI 582 GAGCTC GAGCT^C SexAI 1094 ACCTGGT A^CC[AT]GGT SfiI 24 GGCCACCGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 1202 CCCGGG CCC^GGG SphI 1617 GCATGC GCATG^C SspBI 1275 TGTACA T^GTACA SspI 1838 AATATT AAT^ATT XcmI 690 CCAGTTCCTCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 1202 CCCGGG C^CCGGG XmaI 1202 CCCGGG C^CCGGG XmnI 772 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AfaI 54 GTAC GT^AC 1276 GTAC ApaI 252 GGGCCC GGGCC^C 1205 GGGCCC BclI 147 TGATCA T^GATCA 609 TGATCA BfaI 598 CTAG C^TAG 751 CTAG BglI 25 GCCACCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1815 GCCTCTGGGGC BglII 410 AGATCT A^GATCT 614 AGATCT BsiEI 118 CGGCCG CG[GA][CT]^CG 234 CGACCG DraIII 1093 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG 1587 CACCATGTG DsaI 938 CCATGG C^C[GA][CT]GG 1187 CCATGG FbaI 147 TGATCA T^GATCA 609 TGATCA HphI 1586 TCACC GGTGA(8/7) 1982 TCACC MaeI 598 CTAG C^TAG 751 CTAG NcoI 938 CCATGG C^CATGG 1187 CCATGG NspI 365 ACATGT [GA]CATG^[CT] 1617 GCATGC PflMI 204 CCACTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1775 CCATCTCATGG PpuMI 1472 GGGACCC [GA]G^G[AT]CC[CT] 1658 GGGTCCC RsaI 54 GTAC GT^AC 1276 GTAC SapI 772 GAAGAGC GCTCTTC(1/4) 39 GCTCTTC StuI 1487 AGGCCT AGG^CCT 1757 AGGCCT TaqI 381 TCGA T^CGA 730 TCGA TfiI 494 GATTC G^A[AT]TC 951 GATTC TthHB8I 381 TCGA T^CGA 730 TCGA Van91I 204 CCACTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1775 CCATCTCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 91 enzymes) # ######################################### AccII AciI AcsI AflIII AluI AlwI ApoI AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BcnI BmyI BpmI BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII ItaI Ksp632I MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PstI PvuII Sau3AI Sau96I ScrFI SfaNI SfcI StyI TseI Tsp45I Tsp509I TspRI XhoII