(Linear) (Six-base) MAPSORT of: KIEE2045.seq Check: 4600 from: 1 to: 1989 KIEE2045.orf With 182 enzymes: SgfI * July 15, 2010 18:53 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 553 763 787 928 1975 1989 Size: 553 210 24 141 1047 14 Fragments arranged by size: 1047 553 210 141 24 14 AflIII A'CryG_T Cuts at: 0 221 365 564 1989 Size: 221 144 199 1425 Fragments arranged by size: 1425 221 199 144 AleI CACnn'nnGTG Cuts at: 0 1510 1989 Size: 1510 479 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 262 1989 Size: 262 1727 ApaI G_GGCC'C Cuts at: 0 256 1209 1989 Size: 256 953 780 Fragments arranged by size: 953 780 256 ApaLI G'TGCA_C Cuts at: 0 1605 1989 Size: 1605 384 ApoI r'AATT_y Cuts at: 0 848 1044 1235 1989 Size: 848 196 191 754 Fragments arranged by size: 848 754 196 191 AvaI C'yCGr_G Cuts at: 0 325 762 1202 1989 Size: 325 437 440 787 Fragments arranged by size: 787 440 437 325 BanI G'GyrC_C Cuts at: 0 331 703 1130 1812 1823 1989 Size: 331 372 427 682 11 166 Fragments arranged by size: 682 427 372 331 166 11 BanII G_rGCy'C Cuts at: 0 176 256 546 586 920 1209 1989 Size: 176 80 290 40 334 289 780 Fragments arranged by size: 780 334 290 289 176 80 40 BbsI GAAGACnn'nnnn_ Cuts at: 0 608 1590 1756 1989 Size: 608 982 166 233 Fragments arranged by size: 982 608 233 166 BbvCI CC'TCA_GC Cuts at: 0 1103 1989 Size: 1103 886 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1774 1808 1989 Size: 1774 34 181 Fragments arranged by size: 1774 181 34 BclI T'GATC_A Cuts at: 0 147 609 1989 Size: 147 462 1380 Fragments arranged by size: 1380 462 147 BglI GCCn_nnn'nGGC Cuts at: 0 31 1821 1989 Size: 31 1790 168 Fragments arranged by size: 1790 168 31 BglII A'GATC_T Cuts at: 0 410 614 1989 Size: 410 204 1375 Fragments arranged by size: 1375 410 204 BlpI GC'TnA_GC Cuts at: 0 1301 1989 Size: 1301 688 Bme1580I G_kGCm'C Cuts at: 0 256 334 639 1209 1609 1826 1989 Size: 256 78 305 570 400 217 163 Fragments arranged by size: 570 400 305 256 217 163 78 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 124 278 568 729 811 1989 Size: 124 154 290 161 82 1178 Fragments arranged by size: 1178 290 161 154 124 82 Bpu10I CC'TnA_GC Cuts at: 0 316 580 1103 1478 1989 Size: 316 264 523 375 511 Fragments arranged by size: 523 511 375 316 264 BsaAI yAC'GTr Cuts at: 0 1604 1989 Size: 1604 385 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 127 157 1268 1298 1989 Size: 127 30 1111 30 691 Fragments arranged by size: 1111 691 127 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 148 151 154 517 884 1989 Size: 148 3 3 363 367 1105 Fragments arranged by size: 1105 367 363 148 3 3 BseYI C'CCAG_C Cuts at: 0 293 419 967 1356 1613 1960 1989 Size: 293 126 548 389 257 347 29 Fragments arranged by size: 548 389 347 293 257 126 29 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 85 268 319 1045 1129 1989 Size: 85 183 51 726 84 860 Fragments arranged by size: 860 726 183 85 84 51 BsiEI CG_ry'CG Cuts at: 0 121 237 1989 Size: 121 116 1752 Fragments arranged by size: 1752 121 116 BsiHKAI G_wGCw'C Cuts at: 0 586 1258 1374 1609 1989 Size: 586 672 116 235 380 Fragments arranged by size: 672 586 380 235 116 BsmBI CGTCTCn'nnnn_ Cuts at: 0 717 1989 Size: 717 1272 Bsp1286I G_dGCh'C Cuts at: 0 176 256 334 546 586 639 920 1209 Size: 176 80 78 212 40 53 281 289 Cuts at: 1209 1258 1374 1609 1826 1989 Size: 49 116 235 217 163 Fragments arranged by size: 289 281 235 217 212 176 163 116 80 78 53 49 40 BspHI T'CATG_A Cuts at: 0 150 1989 Size: 150 1839 BsrDI GCAATG_nn' Cuts at: 0 1409 1989 Size: 1409 580 BsrFI r'CCGG_y Cuts at: 0 120 1989 Size: 120 1869 BsrGI T'GTAC_A Cuts at: 0 1275 1989 Size: 1275 714 BssSI C'ACGA_G Cuts at: 0 1063 1989 Size: 1063 926 BstEII G'GTnAC_C Cuts at: 0 440 1989 Size: 440 1549 BstXI CCAn_nnnn'nTGG Cuts at: 0 1016 1422 1731 1989 Size: 1016 406 309 258 Fragments arranged by size: 1016 406 309 258 BstYI r'GATC_y Cuts at: 0 410 614 1067 1989 Size: 410 204 453 922 Fragments arranged by size: 922 453 410 204 BtgI C'CryG_G Cuts at: 0 938 1187 1989 Size: 938 249 802 Fragments arranged by size: 938 802 249 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 438 1095 1989 Size: 438 657 894 Fragments arranged by size: 894 657 438 BtsI GCAGTG_nn' Cuts at: 0 1256 1607 1989 Size: 1256 351 382 Fragments arranged by size: 1256 382 351 DraIII CAC_nnn'GTG Cuts at: 0 1098 1592 1989 Size: 1098 494 397 Fragments arranged by size: 1098 494 397 EaeI y'GGCC_r Cuts at: 0 23 118 1721 1772 1989 Size: 23 95 1603 51 217 Fragments arranged by size: 1603 217 95 51 23 EagI C'GGCC_G Cuts at: 0 118 1989 Size: 118 1871 EarI CTCTTCn'nnn_ Cuts at: 0 46 502 625 767 1447 1989 Size: 46 456 123 142 680 542 Fragments arranged by size: 680 542 456 142 123 46 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 112 1989 Size: 112 1877 EcoICRI GAG'CTC Cuts at: 0 584 1989 Size: 584 1405 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 124 278 553 568 729 763 787 811 Size: 124 154 275 15 161 34 24 24 Cuts at: 811 928 1975 1989 Size: 117 1047 14 Fragments arranged by size: 1047 275 161 154 124 117 34 24 24 15 14 EcoNI CCTnn'n_nnAGG Cuts at: 0 325 1989 Size: 325 1664 EcoO109I rG'GnC_Cy Cuts at: 0 32 252 253 1350 1473 1659 1849 1989 Size: 32 220 1 1097 123 186 190 140 Fragments arranged by size: 1097 220 190 186 140 123 32 1 EcoRI G'AATT_C Cuts at: 0 848 1989 Size: 848 1141 FspI TGC'GCA Cuts at: 0 196 1989 Size: 196 1793 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 395 427 942 974 1059 1091 1989 Size: 395 32 515 32 85 32 898 Fragments arranged by size: 898 515 395 85 32 32 32 HincII GTy'rAC Cuts at: 0 1570 1989 Size: 1570 419 MluI A'CGCG_T Cuts at: 0 221 1989 Size: 221 1768 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1000 1080 1989 Size: 1000 80 909 Fragments arranged by size: 1000 909 80 MscI TGG'CCA Cuts at: 0 25 1723 1774 1989 Size: 25 1698 51 215 Fragments arranged by size: 1698 215 51 25 MslI CAynn'nnrTG Cuts at: 0 19 244 1510 1729 1989 Size: 19 225 1266 219 260 Fragments arranged by size: 1266 260 225 219 19 MspA1I CmG'CkG Cuts at: 0 114 293 1305 1641 1964 1989 Size: 114 179 1012 336 323 25 Fragments arranged by size: 1012 336 323 179 114 25 NaeI GCC'GGC Cuts at: 0 122 1989 Size: 122 1867 NcoI C'CATG_G Cuts at: 0 938 1187 1989 Size: 938 249 802 Fragments arranged by size: 938 802 249 NgoMIV G'CCGG_C Cuts at: 0 120 1989 Size: 120 1869 NspI r_CATG'y Cuts at: 0 369 1621 1989 Size: 369 1252 368 Fragments arranged by size: 1252 369 368 PciI A'CATG_T Cuts at: 0 365 1989 Size: 365 1624 PflMI CCAn_nnn'nTGG Cuts at: 0 210 1781 1989 Size: 210 1571 208 Fragments arranged by size: 1571 210 208 PfoI T'CCnGG_A Cuts at: 0 586 1989 Size: 586 1403 PmlI CAC'GTG Cuts at: 0 1604 1989 Size: 1604 385 PpuMI rG'GwC_Cy Cuts at: 0 1473 1659 1989 Size: 1473 186 330 Fragments arranged by size: 1473 330 186 PspOMI G'GGCC_C Cuts at: 0 252 1205 1989 Size: 252 953 784 Fragments arranged by size: 953 784 252 PstI C_TGCA'G Cuts at: 0 113 182 462 935 1487 1989 Size: 113 69 280 473 552 502 Fragments arranged by size: 552 502 473 280 113 69 PvuII CAG'CTG Cuts at: 0 114 1305 1641 1964 1989 Size: 114 1191 336 323 25 Fragments arranged by size: 1191 336 323 114 25 SacI G_AGCT'C Cuts at: 0 586 1989 Size: 586 1403 SanDI GG'GwC_CC Cuts at: 0 1473 1659 1989 Size: 1473 186 330 Fragments arranged by size: 1473 330 186 SapI GCTCTTCn'nnn_ Cuts at: 0 46 767 1989 Size: 46 721 1222 Fragments arranged by size: 1222 721 46 SexAI A'CCwGG_T Cuts at: 0 1094 1989 Size: 1094 895 SfcI C'TryA_G Cuts at: 0 109 178 458 931 1483 1989 Size: 109 69 280 473 552 506 Fragments arranged by size: 552 506 473 280 109 69 SfiI GGCCn_nnn'nGGCC Cuts at: 0 31 1989 Size: 31 1958 SmaI CCC'GGG Cuts at: 0 1204 1989 Size: 1204 785 SphI G_CATG'C Cuts at: 0 1621 1989 Size: 1621 368 SspI AAT'ATT Cuts at: 0 1840 1989 Size: 1840 149 StuI AGG'CCT Cuts at: 0 1489 1759 1989 Size: 1489 270 230 Fragments arranged by size: 1489 270 230 StyI C'CwwG_G Cuts at: 0 938 1187 1703 1989 Size: 938 249 516 286 Fragments arranged by size: 938 516 286 249 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 285 1550 1710 1989 Size: 285 1265 160 279 Fragments arranged by size: 1265 285 279 160 TatI w'GTAC_w Cuts at: 0 1275 1989 Size: 1275 714 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 208 240 1989 Size: 208 32 1749 Fragments arranged by size: 1749 208 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 697 1989 Size: 697 1292 XmaI C'CCGG_G Cuts at: 0 1202 1989 Size: 1202 787 XmnI GAAnn'nnTTC Cuts at: 0 776 1989 Size: 776 1213 Enzymes that do cut: AcuI AflIII AleI AlfI ApaI ApaLI ApoI AvaI BanI BanII BbsI BbvCI BcgI BclI BglI BglII BlpI Bme1580I BpmI Bpu10I BsaAI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BsrDI BsrFI BsrGI BssSI BstEII BstXI BstYI BtgI BtgZI BtsI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI Hin4I HincII MluI MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NspI PciI PflMI PfoI PmlI PpuMI PspOMI PstI PvuII SacI SanDI SapI SexAI SfcI SfiI SmaI SphI SspI StuI StyI TaqII TatI TstI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AloI AlwNI AscI AseI AsiSI AvrII BaeI BamHI BbeI BciVI BfrBI BmgBI BmrI BmtI BplI BpuEI BsaI BsaBI BsaHI BsaWI BsiWI BsmI BspEI BspMI BsrBI BssHII BstAPI BstBI BstZ17I Bsu36I ClaI CspCI DraI DrdI EcoRV FalI FseI FspAI HaeII HindIII HpaI KasI KpnI MfeI NarI NdeI NheI NotI NruI NsiI PacI PasI PmeI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SbfI ScaI SfoI SgfI SgrAI SmlI SnaBI SpeI SrfI SwaI Tth111I XbaI XhoI ZraI