UMOD ORF clone pFN21AE2038

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE2038

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Clone info.

Species

human

Clone Name

pFN21AE2038 Sequence (with vector) Restriction Map

Product ID

FHC25610

ORF Sequence

DNA sequence (1920 bp)
Protein sequence (640 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB590834]

Gene

KIEE2038

Symbol

UMOD

Alias : ADMCKD2, ADTKD1, FJHN, HNFJ, HNFJ1, MCKD2, THGP, THP

Description

uromodulin, transcript variant 1

Original Clone ID

cs02938

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1920 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 640 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS10583.1 3e-202 100.0 UMOD CCDS61876.1 3.4e-194 95.1 UMOD CCDS10582.2 2.4e-80 52.1 GP2 CCDS42128.1 1.3e-79 51.8 GP2 CCDS45432.1 1.1e-52 51.4 GP2 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR017976 357 371 PR00023 Endoglin/CD105 antigen subgroup IPR017976 384 396 PR00023 Endoglin/CD105 antigen subgroup IPR017976 397 414 PR00023 Endoglin/CD105 antigen subgroup IPR017976 497 512 PR00023 Endoglin/CD105 antigen subgroup IPR017976 516 533 PR00023 Endoglin/CD105 antigen subgroup IPR017976 560 575 PR00023 Endoglin/CD105 antigen subgroup HMMPfam IPR013091 65 99 PF07645 EGF calcium-binding IPR013091 108 148 PF07645 EGF calcium-binding IPR001507 334 584 PF00100 Zona pellucida sperm-binding protein HMMSmart IPR006210 31 64 SM00181 Epidermal growth factor-like IPR001881 65 107 SM00179 EGF-like calcium-binding IPR006210 68 107 SM00181 Epidermal growth factor-like IPR001881 108 149 SM00179 EGF-like calcium-binding IPR006210 111 149 SM00181 Epidermal growth factor-like IPR001507 334 585 SM00241 Zona pellucida sperm-binding protein ProfileScan IPR000742 28 64 PS50026 Epidermal growth factor-like IPR000742 65 107 PS50026 Epidermal growth factor-like IPR000742 108 149 PS50026 Epidermal growth factor-like IPR001507 334 589 PS51034 Zona pellucida sperm-binding protein
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 4 PSLTWMLMVVVASWFITTAATDT 26 PRIMARY 23 2 616 AFSSLGLLKVWLPLLLSATLTL 637 PRIMARY 22
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_001008390 640 162000,191845,603860,609886 100.0 100.0 NP_003352 640 162000,191845,603860,609886 100.0 100.0 XP_016879130 668 162000,191845,603860,609886 100.0 100.0 NP_001265543 673 162000,191845,603860,609886 95.1 99.8 XP_011544238 687 162000,191845,603860,609886 93.0 97.7 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE2038 Download

>KIEE2038  1920 bp
ATGGGGCAGCCATCTCTGACTTGGATGCTGATGGTGGTGGTGGCCTCTTGGTTCATCACA
ACTGCAGCCACTGACACCTCAGAAGCAAGATGGTGCTCTGAATGTCACAGCAATGCCACC
TGCACGGAGGATGAGGCCGTTACGACGTGCACCTGTCAGGAGGGCTTCACCGGCGATGGC
CTGACCTGCGTGGACCTGGATGAGTGCGCCATTCCTGGAGCTCACAACTGCTCCGCCAAC
AGCAGCTGCGTAAACACGCCAGGCTCCTTCTCCTGCGTCTGCCCCGAAGGCTTCCGCCTG
TCGCCCGGTCTCGGCTGCACAGACGTGGATGAGTGCGCTGAGCCTGGGCTTAGCCACTGC
CACGCCCTGGCCACATGTGTCAATGTGGTGGGCAGCTACTTGTGCGTATGCCCCGCGGGC
TACCGGGGGGATGGATGGCACTGTGAGTGCTCCCCGGGCTCCTGCGGGCCGGGGTTGGAC
TGCGTGCCCGAGGGCGACGCGCTCGTGTGCGCGGATCCGTGTCAGGCGCACCGCACCCTG
GACGAGTACTGGCGCAGCACCGAGTACGGGGAGGGCTACGCCTGCGACACGGACCTGCGC
GGCTGGTACCGCTTCGTGGGCCAGGGCGGTGCGCGCATGGCCGAGACCTGCGTGCCAGTC
CTGCGCTGCAACACGGCCGCCCCCATGTGGCTCAATGGCACGCATCCGTCCAGCGACGAG
GGCATCGTGAGCCGCAAGGCCTGCGCGCACTGGAGCGGCCACTGCTGCCTGTGGGATGCG
TCCGTCCAGGTGAAGGCCTGTGCCGGCGGCTACTACGTCTACAACCTGACAGCGCCCCCC
GAGTGTCACCTGGCGTACTGCACAGACCCCAGCTCCGTGGAGGGGACGTGTGAGGAGTGC
AGTATAGACGAGGACTGCAAATCGAATAATGGCAGATGGCACTGCCAGTGCAAACAGGAC
TTCAACATCACTGATATCTCCCTCCTGGAGCACAGGCTGGAATGTGGGGCCAATGACATG
AAGGTGTCGCTGGGCAAGTGCCAGCTGAAGAGTCTGGGCTTCGACAAGGTCTTCATGTAC
CTGAGTGACAGCCGGTGCTCGGGCTTCAATGACAGAGACAACCGGGACTGGGTGTCTGTA
GTGACCCCAGCCCGGGATGGCCCCTGTGGGACAGTGTTGACGAGGAATGAAACCCATGCC
ACTTACAGCAACACCCTCTACCTGGCAGATGAGATCATCATCCGTGACCTCAACATCAAA
ATCAACTTTGCATGCTCCTACCCCCTGGACATGAAAGTCAGCCTGAAGACCGCCCTACAG
CCAATGGTCAGTGCTCTAAACATCAGAGTGGGCGGGACCGGCATGTTCACCGTGCGGATG
GCGCTCTTCCAGACCCCTTCCTACACGCAGCCCTACCAAGGCTCCTCCGTGACACTGTCC
ACTGAGGCTTTTCTCTACGTGGGCACCATGTTGGATGGGGGCGACCTGTCCCGATTTGCA
CTGCTCATGACCAACTGCTATGCCACACCCAGTAGCAATGCCACGGACCCCCTGAAGTAC
TTCATCATCCAGGACAGATGCCCACACACTAGAGACTCAACTATCCAAGTGGTGGAGAAT
GGGGAGTCCTCCCAGGGCCGATTTTCCGTCCAGATGTTCCGGTTTGCTGGAAACTATGAC
CTAGTCTACCTGCACTGTGAAGTCTATCTCTGTGACACCATGAATGAAAAGTGCAAGCCT
ACCTGCTCTGGGACCAGATTCCGAAGTGGGAGTGTCATAGATCAATCCCGTGTCCTGAAC
TTGGGTCCCATCACACGGAAAGGTGTCCAGGCCACAGTCTCAAGGGCTTTTAGCAGCTTG
GGGCTCCTGAAAGTCTGGCTGCCTCTGCTTCTCTCGGCCACCTTGACCCTGACTTTTCAG

ORF Protein

Cloned ORF protein sequence for pFN21AE2038 Download

>KIEE2038  640 aa
MGQPSLTWMLMVVVASWFITTAATDTSEARWCSECHSNATCTEDEAVTTCTCQEGFTGDG
LTCVDLDECAIPGAHNCSANSSCVNTPGSFSCVCPEGFRLSPGLGCTDVDECAEPGLSHC
HALATCVNVVGSYLCVCPAGYRGDGWHCECSPGSCGPGLDCVPEGDALVCADPCQAHRTL
DEYWRSTEYGEGYACDTDLRGWYRFVGQGGARMAETCVPVLRCNTAAPMWLNGTHPSSDE
GIVSRKACAHWSGHCCLWDASVQVKACAGGYYVYNLTAPPECHLAYCTDPSSVEGTCEEC
SIDEDCKSNNGRWHCQCKQDFNITDISLLEHRLECGANDMKVSLGKCQLKSLGFDKVFMY
LSDSRCSGFNDRDNRDWVSVVTPARDGPCGTVLTRNETHATYSNTLYLADEIIIRDLNIK
INFACSYPLDMKVSLKTALQPMVSALNIRVGGTGMFTVRMALFQTPSYTQPYQGSSVTLS
TEAFLYVGTMLDGGDLSRFALLMTNCYATPSSNATDPLKYFIIQDRCPHTRDSTIQVVEN
GESSQGRFSVQMFRFAGNYDLVYLHCEVYLCDTMNEKCKPTCSGTRFRSGSVIDQSRVLN
LGPITRKGVQATVSRAFSSLGLLKVWLPLLLSATLTLTFQ

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE2038 Download

>pFN21AE2038  6625 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGGGCAGCCATCTCTGACTTGGATGCTGATGGT
GGTGGTGGCCTCTTGGTTCATCACAACTGCAGCCACTGACACCTCAGAAGCAAGATGGTG
CTCTGAATGTCACAGCAATGCCACCTGCACGGAGGATGAGGCCGTTACGACGTGCACCTG
TCAGGAGGGCTTCACCGGCGATGGCCTGACCTGCGTGGACCTGGATGAGTGCGCCATTCC
TGGAGCTCACAACTGCTCCGCCAACAGCAGCTGCGTAAACACGCCAGGCTCCTTCTCCTG
CGTCTGCCCCGAAGGCTTCCGCCTGTCGCCCGGTCTCGGCTGCACAGACGTGGATGAGTG
CGCTGAGCCTGGGCTTAGCCACTGCCACGCCCTGGCCACATGTGTCAATGTGGTGGGCAG
CTACTTGTGCGTATGCCCCGCGGGCTACCGGGGGGATGGATGGCACTGTGAGTGCTCCCC
GGGCTCCTGCGGGCCGGGGTTGGACTGCGTGCCCGAGGGCGACGCGCTCGTGTGCGCGGA
TCCGTGTCAGGCGCACCGCACCCTGGACGAGTACTGGCGCAGCACCGAGTACGGGGAGGG
CTACGCCTGCGACACGGACCTGCGCGGCTGGTACCGCTTCGTGGGCCAGGGCGGTGCGCG
CATGGCCGAGACCTGCGTGCCAGTCCTGCGCTGCAACACGGCCGCCCCCATGTGGCTCAA
TGGCACGCATCCGTCCAGCGACGAGGGCATCGTGAGCCGCAAGGCCTGCGCGCACTGGAG
CGGCCACTGCTGCCTGTGGGATGCGTCCGTCCAGGTGAAGGCCTGTGCCGGCGGCTACTA
CGTCTACAACCTGACAGCGCCCCCCGAGTGTCACCTGGCGTACTGCACAGACCCCAGCTC
CGTGGAGGGGACGTGTGAGGAGTGCAGTATAGACGAGGACTGCAAATCGAATAATGGCAG
ATGGCACTGCCAGTGCAAACAGGACTTCAACATCACTGATATCTCCCTCCTGGAGCACAG
GCTGGAATGTGGGGCCAATGACATGAAGGTGTCGCTGGGCAAGTGCCAGCTGAAGAGTCT
GGGCTTCGACAAGGTCTTCATGTACCTGAGTGACAGCCGGTGCTCGGGCTTCAATGACAG
AGACAACCGGGACTGGGTGTCTGTAGTGACCCCAGCCCGGGATGGCCCCTGTGGGACAGT
GTTGACGAGGAATGAAACCCATGCCACTTACAGCAACACCCTCTACCTGGCAGATGAGAT
CATCATCCGTGACCTCAACATCAAAATCAACTTTGCATGCTCCTACCCCCTGGACATGAA
AGTCAGCCTGAAGACCGCCCTACAGCCAATGGTCAGTGCTCTAAACATCAGAGTGGGCGG
GACCGGCATGTTCACCGTGCGGATGGCGCTCTTCCAGACCCCTTCCTACACGCAGCCCTA
CCAAGGCTCCTCCGTGACACTGTCCACTGAGGCTTTTCTCTACGTGGGCACCATGTTGGA
TGGGGGCGACCTGTCCCGATTTGCACTGCTCATGACCAACTGCTATGCCACACCCAGTAG
CAATGCCACGGACCCCCTGAAGTACTTCATCATCCAGGACAGATGCCCACACACTAGAGA
CTCAACTATCCAAGTGGTGGAGAATGGGGAGTCCTCCCAGGGCCGATTTTCCGTCCAGAT
GTTCCGGTTTGCTGGAAACTATGACCTAGTCTACCTGCACTGTGAAGTCTATCTCTGTGA
CACCATGAATGAAAAGTGCAAGCCTACCTGCTCTGGGACCAGATTCCGAAGTGGGAGTGT
CATAGATCAATCCCGTGTCCTGAACTTGGGTCCCATCACACGGAAAGGTGTCCAGGCCAC
AGTCTCAAGGGCTTTTAGCAGCTTGGGGCTCCTGAAAGTCTGGCTGCCTCTGCTTCTCTC
GGCCACCTTGACCCTGACTTTTCAGGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCC
TCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAG
CTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTT
CGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGA
AAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGC
TGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAG
ATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAA
TGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATC
CGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGT
GGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCA
AATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGG
AAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGC
CTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTG
GGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTT
CGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTA
TTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAAT
GACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGA
GAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACA
ACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACT
CGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACC
ACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACT
CTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTT
CTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGT
GGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTT
ATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATA
GGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGG
TATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAA
AGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGG
CGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGA
GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCG
TGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGG
GAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTC
GCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCG
GTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCA
CTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGT
GGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAG
TTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCG
GTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATC
CTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTT
TGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAG
GAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAG
TGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTA
AAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTC
TGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAA
ATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATG
CGTCCGGCGTAGAGGATCGAGATCT