(Linear) (Six-base) MAPSORT of: KIEE2038.seq Check: 6442 from: 1 to: 1920 KIEE2038.orf With 182 enzymes: SgfI * July 15, 2010 18:53 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 127 1920 Size: 127 1793 AccI GT'mk_AC Cuts at: 0 818 1685 1920 Size: 818 867 235 Fragments arranged by size: 867 818 235 Acc65I G'GTAC_C Cuts at: 0 605 1920 Size: 605 1315 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1066 1324 1573 1920 Size: 1066 258 249 347 Fragments arranged by size: 1066 347 258 249 AflIII A'CryG_T Cuts at: 0 373 886 1920 Size: 373 513 1034 Fragments arranged by size: 1034 513 373 AhdI GACnn_n'nnGTC Cuts at: 0 1131 1920 Size: 1131 789 AleI CACnn'nnGTG Cuts at: 0 322 1920 Size: 322 1598 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 108 1920 Size: 108 1812 AlwNI CAG_nnn'CTG Cuts at: 0 70 245 1920 Size: 70 175 1675 Fragments arranged by size: 1675 175 70 ApaLI G'TGCA_C Cuts at: 0 147 1920 Size: 147 1773 AvaI C'yCGr_G Cuts at: 0 453 487 838 1098 1151 1920 Size: 453 34 351 260 53 769 Fragments arranged by size: 769 453 351 260 53 34 BamHI G'GATC_C Cuts at: 0 513 1920 Size: 513 1407 BanI G'GyrC_C Cuts at: 0 605 1462 1920 Size: 605 857 458 Fragments arranged by size: 857 605 458 BanII G_rGCy'C Cuts at: 0 222 460 1865 1920 Size: 222 238 1405 55 Fragments arranged by size: 1405 238 222 55 BbsI GAAGACnn'nnnn_ Cuts at: 0 1062 1312 1920 Size: 1062 250 608 Fragments arranged by size: 1062 608 250 BglI GCCn_nnn'nGGC Cuts at: 0 287 416 1920 Size: 287 129 1504 Fragments arranged by size: 1504 287 129 BlpI GC'TnA_GC Cuts at: 0 338 349 1920 Size: 338 11 1571 Fragments arranged by size: 1571 338 11 Bme1580I G_kGCm'C Cuts at: 0 151 488 1465 1920 Size: 151 337 977 455 Fragments arranged by size: 977 455 337 151 BmgBI CAC'GTC Cuts at: 0 146 324 887 1920 Size: 146 178 563 1033 Fragments arranged by size: 1033 563 178 146 BmrI ACTGGGnnnn_n' Cuts at: 0 1137 1523 1920 Size: 1137 386 397 Fragments arranged by size: 1137 397 386 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 236 771 1006 1920 Size: 236 535 235 914 Fragments arranged by size: 914 535 236 235 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1824 1920 Size: 1824 96 BsaI GGTCTCn'nnnn_ Cuts at: 0 313 637 1920 Size: 313 324 1283 Fragments arranged by size: 1283 324 313 BsaAI yAC'GTr Cuts at: 0 1458 1920 Size: 1458 462 BsaBI GATnn'nnATC Cuts at: 0 1237 1920 Size: 1237 683 BsaWI w'CCGG_w Cuts at: 0 1658 1920 Size: 1658 262 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 214 244 856 871 886 901 1405 1435 Size: 214 30 612 15 15 15 504 30 Cuts at: 1435 1920 Size: 485 Fragments arranged by size: 612 504 485 214 30 30 15 15 15 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 907 1413 1920 Size: 907 506 507 Fragments arranged by size: 907 507 506 BseYI C'CCAG_C Cuts at: 0 868 1029 1146 1920 Size: 868 161 117 774 Fragments arranged by size: 868 774 161 117 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 105 300 843 918 1675 1920 Size: 105 195 543 75 757 245 Fragments arranged by size: 757 543 245 195 105 75 BsiEI CG_ry'CG Cuts at: 0 677 1920 Size: 677 1243 BsiHKAI G_wGCw'C Cuts at: 0 97 151 222 451 992 1099 1335 1920 Size: 97 54 71 229 541 107 236 585 Fragments arranged by size: 585 541 236 229 107 97 71 54 Bsp1286I G_dGCh'C Cuts at: 0 97 151 222 451 460 488 992 1099 Size: 97 54 71 229 9 28 504 107 Cuts at: 1099 1335 1465 1865 1920 Size: 236 130 400 55 Fragments arranged by size: 504 400 236 229 130 107 97 71 55 54 28 9 BspHI T'CATG_A Cuts at: 0 1505 1920 Size: 1505 415 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 127 193 602 655 1697 1750 1920 Size: 127 66 409 53 1042 53 170 Fragments arranged by size: 1042 409 170 127 66 53 53 BsrBI CCG'CTC Cuts at: 0 755 1920 Size: 755 1165 BsrDI GCAATG_nn' Cuts at: 0 117 1542 1920 Size: 117 1425 378 Fragments arranged by size: 1425 378 117 BsrFI r'CCGG_y Cuts at: 0 169 802 1091 1357 1920 Size: 169 633 289 266 563 Fragments arranged by size: 633 563 289 266 169 BssHII G'CGCG_C Cuts at: 0 631 743 1920 Size: 631 112 1177 Fragments arranged by size: 1177 631 112 BssSI C'ACGA_G Cuts at: 0 502 1920 Size: 502 1418 BstAPI GCAn_nnn'nTGC Cuts at: 0 740 1920 Size: 740 1180 BstXI CCAn_nnnn'nTGG Cuts at: 0 876 1920 Size: 876 1044 BstYI r'GATC_y Cuts at: 0 513 1920 Size: 513 1407 BtgI C'CryG_G Cuts at: 0 413 875 1541 1920 Size: 413 462 666 379 Fragments arranged by size: 666 462 413 379 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 188 1920 Size: 188 1732 BtsI GCAGTG_nn' Cuts at: 0 354 759 939 1498 1920 Size: 354 405 180 559 422 Fragments arranged by size: 559 422 405 354 180 DrdI GACnn_nn'nnGTC Cuts at: 0 1294 1920 Size: 1294 626 EaeI y'GGCC_r Cuts at: 0 368 638 674 756 1895 1920 Size: 368 270 36 82 1139 25 Fragments arranged by size: 1139 368 270 82 36 25 EagI C'GGCC_G Cuts at: 0 674 1920 Size: 674 1246 EarI CTCTTCn'nnn_ Cuts at: 0 1042 1390 1920 Size: 1042 348 530 Fragments arranged by size: 1042 530 348 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 222 283 1920 Size: 222 61 1637 Fragments arranged by size: 1637 222 61 EcoICRI GAG'CTC Cuts at: 0 220 1920 Size: 220 1700 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 236 771 1006 1066 1324 1573 1920 Size: 236 535 235 60 258 249 347 Fragments arranged by size: 535 347 258 249 236 235 60 EcoO109I rG'GnC_Cy Cuts at: 0 1804 1920 Size: 1804 116 EcoRV GAT'ATC Cuts at: 0 975 1920 Size: 975 945 HaeII r_GCGC'y Cuts at: 0 835 1384 1920 Size: 835 549 536 Fragments arranged by size: 835 549 536 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 856 888 904 936 1117 1149 1631 1663 Size: 856 32 16 32 181 32 482 32 Cuts at: 1663 1920 Size: 257 Fragments arranged by size: 856 482 257 181 32 32 32 32 16 HincII GTy'rAC Cuts at: 0 1178 1920 Size: 1178 742 KpnI G_GTAC'C Cuts at: 0 609 1920 Size: 609 1311 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 455 1451 1920 Size: 455 996 469 Fragments arranged by size: 996 469 455 MscI TGG'CCA Cuts at: 0 370 1920 Size: 370 1550 MslI CAynn'nnrTG Cuts at: 0 110 322 1021 1345 1920 Size: 110 212 699 324 575 Fragments arranged by size: 699 575 324 212 110 MspA1I CmG'CkG Cuts at: 0 245 415 1044 1920 Size: 245 170 629 876 Fragments arranged by size: 876 629 245 170 NaeI GCC'GGC Cuts at: 0 804 1920 Size: 804 1116 NgoMIV G'CCGG_C Cuts at: 0 802 1920 Size: 802 1118 NspI r_CATG'y Cuts at: 0 377 1274 1365 1920 Size: 377 897 91 555 Fragments arranged by size: 897 555 377 91 PasI CC'CwG_GG Cuts at: 0 1632 1920 Size: 1632 288 PciI A'CATG_T Cuts at: 0 373 1920 Size: 373 1547 PflMI CCAn_nnn'nTGG Cuts at: 0 366 1611 1920 Size: 366 1245 309 Fragments arranged by size: 1245 366 309 PfoI T'CCnGG_A Cuts at: 0 213 983 1568 1920 Size: 213 770 585 352 Fragments arranged by size: 770 585 352 213 PpuMI rG'GwC_Cy Cuts at: 0 1804 1920 Size: 1804 116 PstI C_TGCA'G Cuts at: 0 66 1920 Size: 66 1854 PvuII CAG'CTG Cuts at: 0 245 1044 1920 Size: 245 799 876 Fragments arranged by size: 876 799 245 SacI G_AGCT'C Cuts at: 0 222 1920 Size: 222 1698 SacII CC_GC'GG Cuts at: 0 416 1920 Size: 416 1504 SanDI GG'GwC_CC Cuts at: 0 1804 1920 Size: 1804 116 SapI GCTCTTCn'nnn_ Cuts at: 0 1390 1920 Size: 1390 530 ScaI AGT'ACT Cuts at: 0 547 1558 1920 Size: 547 1011 362 Fragments arranged by size: 1011 547 362 SfcI C'TryA_G Cuts at: 0 62 1136 1315 1920 Size: 62 1074 179 605 Fragments arranged by size: 1074 605 179 62 SgrAI Cr'CCGG_yG Cuts at: 0 169 1920 Size: 169 1751 SmaI CCC'GGG Cuts at: 0 455 1153 1920 Size: 455 698 767 Fragments arranged by size: 767 698 455 SmlI C'TyrA_G Cuts at: 0 1839 1920 Size: 1839 81 SphI G_CATG'C Cuts at: 0 1274 1920 Size: 1274 646 StuI AGG'CCT Cuts at: 0 739 796 1920 Size: 739 57 1124 Fragments arranged by size: 1124 739 57 StyI C'CwwG_G Cuts at: 0 1416 1920 Size: 1416 504 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1119 1542 1920 Size: 1119 423 378 Fragments arranged by size: 1119 423 378 TatI w'GTAC_w Cuts at: 0 545 1556 1920 Size: 545 1011 364 Fragments arranged by size: 1011 545 364 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 214 246 871 903 1920 Size: 214 32 625 32 1017 Fragments arranged by size: 1017 625 214 32 32 Tth111I GACn'n_nGTC Cuts at: 0 1066 1434 1681 1920 Size: 1066 368 247 239 Fragments arranged by size: 1066 368 247 239 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 17 1920 Size: 17 1903 XmaI C'CCGG_G Cuts at: 0 453 1151 1920 Size: 453 698 769 Fragments arranged by size: 769 698 453 XmnI GAAnn'nnTTC Cuts at: 0 1558 1920 Size: 1558 362 Enzymes that do cut: AarI AccI Acc65I AcuI AflIII AhdI AleI AlfI AlwNI ApaLI AvaI BamHI BanI BanII BbsI BglI BlpI Bme1580I BmgBI BmrI BpmI BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstXI BstYI BtgI BtgZI BtsI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRV HaeII Hin4I HincII KpnI MmeI MscI MslI MspA1I NaeI NgoMIV NspI PasI PciI PflMI PfoI PpuMI PstI PvuII SacI SacII SanDI SapI ScaI SfcI SgrAI SmaI SmlI SphI StuI StyI TaqII TatI TstI Tth111I XcmI XmaI XmnI Enzymes that do not cut: AatII AclI AfeI AflII AgeI AloI ApaI ApoI AscI AseI AsiSI AvrII BaeI BbeI BbvCI BcgI BciVI BclI BfrBI BglII BmtI BplI Bpu10I BsaHI BsiWI BsmI BsmBI BspEI BsrGI BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraI DraIII EcoNI EcoRI FalI FseI FspI FspAI HindIII HpaI KasI MfeI MluI NarI NcoI NdeI NheI NotI NruI NsiI PacI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SalI SbfI SexAI SfiI SfoI SgfI SnaBI SpeI SrfI SspI SwaI XbaI XhoI ZraI