################################## # NO CUT ENZYMES ( 97 enzymes) # ################################## AatII AccIII AclI AcsI AcyI AflII AgeI AocI Aor51HI ApaI ApoI AscI AseI AsnI AviII AvrII BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglII BlnI BsaHI BseAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BsrGI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraI DraIII Eco47III Eco81I EcoNI EcoO65I EcoRI EcoT22I FbaI FseI FspI Hin1I HindIII HpaI KasI MfeI MluI MroI MseI MunI NarI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SalI SbfI SexAI SfiI SfoI SfuI SnaBI SpeI Sse8387I SspBI SspI SwaI Tru9I Tsp509I XbaI XhoI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### Acc65I 605 GGTACC G^GTACC AhdI 1126 GACTGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 147 GTGCAC G^TGCAC ApaLI 147 GTGCAC G^TGCAC Asp700I 1554 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 605 GGTACC G^GTACC AspEI 1126 GACTGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BalI 368 TGGCCA TGG^CCA BamHI 513 GGATCC G^GATCC BsaAI 1456 TACGTG [CT]AC^GT[GA] BsaBI 1233 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 1658 TCCGGT [AT]^CCGG[AT] BsiEI 674 CGGCCG CG[GA][CT]^CG BspHI 1505 TCATGA T^CATGA BspLU11I 373 ACATGT A^CATGT BsrBI 753 GAGCGG CCGCTC(-3/-3) BssSI 502 CTCGTG CACGAG(-5/-1) BstAPI 734 GCAAGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 869 CCAGCTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 513 GGATCC [GA]^GATC[CT] DraII 1803 GGGTCCC [GA]G^G[ACGT]CC[CT] DrdI 1288 GACATGAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 674 CGGCCG C^GGCCG Eam1105I 1126 GACTGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 674 CGGCCG C^GGCCG Eco52I 674 CGGCCG C^GGCCG EcoO109I 1803 GGGTCCC [GA]G^G[ACGT]CC[CT] EcoRV 973 GATATC GAT^ATC EcoT14I 1416 CCAAGG C^C[AT][AT]GG HincII 1176 GTTGAC GT[CT]^[GA]AC HindII 1176 GTTGAC GT[CT]^[GA]AC KpnI 605 GGTACC GGTAC^C KspI 413 CCGCGG CCGC^GG MamI 1233 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 513 GGATCC [GA]^GATC[CT] MluNI 368 TGGCCA TGG^CCA MscI 368 TGGCCA TGG^CCA NaeI 802 GCCGGC GCC^GGC NgoMIV 802 GCCGGC G^CCGGC PpuMI 1803 GGGTCCC [GA]G^G[AT]CC[CT] PstI 62 CTGCAG CTGCA^G RcaI 1505 TCATGA T^CATGA SacI 218 GAGCTC GAGCT^C SacII 413 CCGCGG CCGC^GG SapI 1383 GCTCTTC GCTCTTC(1/4) SgrAI 168 CACCGGCG C[GA]^CCGG[CT]G SmlI 1839 CTCAAG C^T[CT][GA]AG SphI 1270 GCATGC GCATG^C StyI 1416 CCAAGG C^C[AT][AT]GG TfiI 1757 GATTC G^A[AT]TC XcmI 10 CCATCTCTGACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 513 GGATCC [GA]^GATC[CT] XmnI 1554 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AccI 817 GTCTAC GT^[AC][GT]AC 1684 GTCTAC AflIII 373 ACATGT A^C[GA][CT]GT 886 ACGTGT AlwI 514 GATCC GGATC(4/5) 513 GGATC AlwNI 65 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG 240 CAGCAGCTG BanI 605 GGTACC G^G[CT][GA]CC 1462 GGCACC BbsI 1305 GAAGAC GAAGAC(2/6) 1069 GTCTTC BfaI 1589 CTAG C^TAG 1681 CTAG BglI 281 GCCCCGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 410 GCCCCGCGGGC BlpI 337 GCTGAGC GC^T[ACGT]AGC 348 GCTTAGC Bpu1102I 337 GCTGAGC GC^T[ACGT]AGC 348 GCTTAGC BpuAI 1305 GAAGAC GAAGAC(2/6) 1069 GTCTTC BsaI 643 GAGACC GGTCTC(1/5) 307 GGTCTC BseRI 1422 CTCCTC GAGGAG(10/8) 892 GAGGAG BsrDI 110 GCAATG GCAATG(2/0) 1535 GCAATG BssHII 631 GCGCGC G^CGCGC 743 GCGCGC CelII 337 GCTGAGC GC^T[ACGT]AGC 348 GCTTAGC EarI 1384 CTCTTC CTCTTC(1/4) 1047 GAAGAG HaeII 831 AGCGCC [GA]GCGC^[CT] 1380 GGCGCT Ksp632I 1384 CTCTTC CTCTTC(1/4) 1047 GAAGAG MaeI 1589 CTAG C^TAG 1681 CTAG PflMI 360 CCACGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1605 CCAAGTGGTGG PvuII 243 CAGCTG CAG^CTG 1042 CAGCTG ScaI 545 AGTACT AGT^ACT 1556 AGTACT SmaI 453 CCCGGG CCC^GGG 1151 CCCGGG StuI 737 AGGCCT AGG^CCT 794 AGGCCT TaqI 922 TCGA T^CGA 1061 TCGA TthHB8I 922 TCGA T^CGA 1061 TCGA Van91I 360 CCACGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1605 CCAAGTGGTGG XmaCI 453 CCCGGG C^CCGGG 1151 CCCGGG XmaI 453 CCCGGG C^CCGGG 1151 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 78 enzymes) # ######################################### AccII AciI AfaI AluI AspHI AspI AvaI AvaII BanII BbvI BcnI BmyI BpmI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DsaI EaeI EcoRII FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflFI PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TseI Tsp45I TspRI Tth111I