Restriction map for
pFN21AE2038
(Circular) (Six-base) MAPSORT of: pFN21AE2038.seq Check: 4523 from: 1 to: 6625
pFN21AE2038 6625 bp
With 182 enzymes: SgfI *
August 16, 2010 14:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2132 2132
Size: 6625
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4435 278
Size: 53 83 186 936 2899 2468
Fragments arranged by size:
2899 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2823 3690 3968 1937
Size: 886 867 278 4594
Fragments arranged by size:
4594 886 867 278
Acc65I G'GTAC_C
Cuts at: 2610 3946 2610
Size: 1336 5289
AclI AA'CG_TT
Cuts at: 1563 4752 5125 1563
Size: 3189 373 3063
Fragments arranged by size:
3189 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3071 3329 3578 4688 6072 6340 1632
Size: 1439 258 249 1110 1384 268 1917
Fragments arranged by size:
1917 1439 1384 1110 268 258 249
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6428
AflIII A'CryG_T
Cuts at: 1203 2378 2891 5524 6501 1203
Size: 1175 513 2633 977 1327
Fragments arranged by size:
2633 1327 1175 977 513
AgeI A'CCGG_T
Cuts at: 5456 5456
Size: 6625
AhdI GACnn_n'nnGTC
Cuts at: 3136 5354 3136
Size: 2218 4407
AleI CACnn'nnGTG
Cuts at: 1166 2327 1166
Size: 1161 5464
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2113 1184
Size: 929 5696
AlwNI CAG_nnn'CTG
Cuts at: 2075 2250 4368 5940 2075
Size: 175 2118 1572 2760
Fragments arranged by size:
2760 2118 1572 175
ApaLI G'TGCA_C
Cuts at: 2152 4682 5838 2152
Size: 2530 1156 2939
Fragments arranged by size:
2939 2530 1156
ApoI r'AATT_y
Cuts at: 1493 3934 4160 4312 6421 1493
Size: 2441 226 152 2109 1697
Fragments arranged by size:
2441 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5179 160
Size: 872 4147 1606
Fragments arranged by size:
4147 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6625
AvaI C'yCGr_G
Cuts at: 1870 1943 2458 2492 2843 3103 3156 3950 1870
Size: 73 515 34 351 260 53 794 4545
Fragments arranged by size:
4545 794 515 351 260 73 53 34
BamHI G'GATC_C
Cuts at: 2518 3955 4322 2518
Size: 1437 367 4821
Fragments arranged by size:
4821 1437 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2610 3467 3946 5401 618
Size: 325 217 627 823 857 479 1455 1842
Fragments arranged by size:
1842 1455 857 823 627 479 325 217
BanII G_rGCy'C
Cuts at: 1391 2227 2465 3870 3944 1391
Size: 836 238 1405 74 4072
Fragments arranged by size:
4072 1405 836 238 74
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3067 3317 928
Size: 971 1168 250 4236
Fragments arranged by size:
4236 1168 971 250
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4816 4850 1903
Size: 34 2879 34 3678
Fragments arranged by size:
3678 2879 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4517 5727 4517
Size: 1210 5415
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6625
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2292 2421 5236
Size: 107 122 71 631 605 620 129 2815
Cuts at: 5236 136
Size: 1525
Fragments arranged by size:
2815 1525 631 620 605 129 122 107
71
BglII A'GATC_T
Cuts at: 6620 6620
Size: 6625
BlpI GC'TnA_GC
Cuts at: 2343 2354 4042 2343
Size: 11 1688 4926
Fragments arranged by size:
4926 1688 11
Bme1580I G_kGCm'C
Cuts at: 1790 2156 2493 3470 4686 5842 1790
Size: 366 337 977 1216 1156 2573
Fragments arranged by size:
2573 1216 1156 977 366 337
BmgBI CAC'GTC
Cuts at: 1318 2151 2329 2892 1318
Size: 833 178 563 5051
Fragments arranged by size:
5051 833 563 178
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3142 3528 4356
Size: 416 53 83 399 24 1725 386 828
Cuts at: 4356 5314 442
Size: 958 1753
Fragments arranged by size:
1753 1725 958 828 416 399 386 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6625
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2241 2776 3011 5285 1239
Size: 1002 535 235 2274 2579
Fragments arranged by size:
2579 2274 1002 535 235
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3829 4750 5615 5913 3829
Size: 921 865 298 4541
Fragments arranged by size:
4541 921 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2318 2642 5288 882
Size: 617 819 324 2646 2219
Fragments arranged by size:
2646 2219 819 617 324
BsaAI yAC'GTr
Cuts at: 493 1204 3463 4416 493
Size: 711 2259 953 2702
Fragments arranged by size:
2702 2259 953 711
BsaBI GATnn'nnATC
Cuts at: 3242 6619 3242
Size: 3377 3248
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4432 4814 5449 275
Size: 53 83 186 936 2899 382 635 1451
Fragments arranged by size:
2899 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3663 5058 5456 5730 5877 6288 3663
Size: 1395 398 274 147 411 4000
Fragments arranged by size:
4000 1395 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2219 2249 2861 2876 2891
Size: 30 165 30 816 30 612 15 15
Cuts at: 2891 2906 3410 3440 1178
Size: 15 504 30 4363
Fragments arranged by size:
4363 816 612 504 165 30 30 30
30 15 15 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2912 3418 1186
Size: 1726 506 4393
Fragments arranged by size:
4393 1726 506
BseYI C'CCAG_C
Cuts at: 2873 3034 3151 5828 6324 2873
Size: 161 117 2677 496 3174
Fragments arranged by size:
3174 2677 496 161 117
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2110 2305 2848 2923 3680 1163
Size: 573 374 195 543 75 757 4108
Fragments arranged by size:
4108 757 573 543 374 195 75
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2682 4075 4836 5864 1540
Size: 275 186 681 1393 761 1028 2301
Fragments arranged by size:
2301 1393 1028 761 681 275 186
BsiHKAI G_wGCw'C
Cuts at: 2102 2156 2227 2456 2997 3104 3340 4686 4771
Size: 54 71 229 541 107 236 1346 85
Cuts at: 4771 5842 2102
Size: 1071 2885
Fragments arranged by size:
2885 1346 1071 541 236 229 107 85
71 54
BsmI GAATG_Cn'
Cuts at: 4136 4229 4136
Size: 93 6532
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2102 2156 2227 2456 2465 2493 2997
Size: 399 312 54 71 229 9 28 504
Cuts at: 2997 3104 3340 3470 3870 3944 4686 4771 5842
Size: 107 236 130 400 74 742 85 1071
Cuts at: 5842 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 504 400 399 312 236
229 130 107 85 74 71 54 28
9
BspEI T'CCGG_A
Cuts at: 6288 6288
Size: 6625
BspHI T'CATG_A
Cuts at: 3510 4514 6244 3510
Size: 1004 1730 3891
Fragments arranged by size:
3891 1730 1004
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2132 2198 2607 2660 3702 3755 3980 844
Size: 1288 66 409 53 1042 53 225 3489
Fragments arranged by size:
3489 1288 1042 409 225 66 53 53
BsrBI CCG'CTC
Cuts at: 1394 1823 2760 4512 1394
Size: 429 937 1752 3507
Fragments arranged by size:
3507 1752 937 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2122 3547 5120 5294 65
Size: 1183 874 1425 1573 174 1396
Fragments arranged by size:
1573 1425 1396 1183 874 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2174 2807 3096 3362 5269 5456 1700
Size: 110 364 633 289 266 1907 187 2869
Fragments arranged by size:
2869 1907 633 364 289 266 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6625
BssHII G'CGCG_C
Cuts at: 1925 2636 2748 1925
Size: 711 112 5802
Fragments arranged by size:
5802 711 112
BssSI C'ACGA_G
Cuts at: 2507 4685 5697 2507
Size: 2178 1012 3435
Fragments arranged by size:
3435 2178 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2745 794
Size: 1951 4674
BstBI TT'CG_AA
Cuts at: 5429 5429
Size: 6625
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6625
BstXI CCAn_nnnn'nTGG
Cuts at: 2881 2881
Size: 6625
BstYI r'GATC_y
Cuts at: 1972 2518 3955 4322 4707 4724 6176 6262 6274
Size: 546 1437 367 385 17 1452 86 12
Cuts at: 6274 6620 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1437 546 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2418 2880 3546 513
Size: 552 939 414 462 666 3592
Fragments arranged by size:
3592 939 666 552 462 414
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2193 487
Size: 683 56 967 4919
Fragments arranged by size:
4919 967 683 56
BtsI GCAGTG_nn'
Cuts at: 2359 2764 2944 3503 4141 4935 4955 2359
Size: 405 180 559 638 794 20 4029
Fragments arranged by size:
4029 794 638 559 405 180 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6625
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6590
DraI TTT'AAA
Cuts at: 3929 3929
Size: 6625
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3299 5632 809
Size: 507 1983 2333 1802
Fragments arranged by size:
2333 1983 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2373 2643 2679
Size: 54 1425 50 275 18 543 270 36
Cuts at: 2679 2761 3900 4072 4961 6590 8
Size: 82 1139 172 889 1629 43
Fragments arranged by size:
1629 1425 1139 889 543 275 270 172
82 54 50 43 36 18
EagI C'GGCC_G
Cuts at: 1537 1812 2679 4072 1537
Size: 275 867 1393 4090
Fragments arranged by size:
4090 1393 867 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3047 3395 4555 1353
Size: 1694 348 1160 3423
Fragments arranged by size:
3423 1694 1160 348
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2227 2288 5212 5586 5732 387
Size: 1064 138 638 61 2924 374 146 1280
Fragments arranged by size:
2924 1280 1064 638 374 146 138 61
EcoICRI GAG'CTC
Cuts at: 2225 2225
Size: 6625
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2241 2776 3011 3071 3329 3578 4688
Size: 393 609 535 235 60 258 249 1110
Cuts at: 4688 5285 6072 6340 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1110 787 609 597 535 393 268
258 249 235 60
EcoO109I rG'GnC_Cy
Cuts at: 3809 3809
Size: 6625
EcoRI G'AATT_C
Cuts at: 3934 4312 3934
Size: 378 6247
EcoRV GAT'ATC
Cuts at: 2980 2980
Size: 6625
FspI TGC'GCA
Cuts at: 5131 5131
Size: 6625
HaeII r_GCGC'y
Cuts at: 1678 2840 3389 5772 1678
Size: 1162 549 2383 2531
Fragments arranged by size:
2531 2383 1162 549
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2861 2893 2909 2941 3122
Size: 32 135 32 1289 32 16 32 181
Cuts at: 3122 3154 3636 3668 5266 5298 5340 5372 1373
Size: 32 482 32 1598 32 42 32 2626
Fragments arranged by size:
2626 1598 1289 482 181 135 42 32
32 32 32 32 32 32 32 16
HincII GTy'rAC
Cuts at: 669 1657 1938 3183 3969 4215 669
Size: 988 281 1245 786 246 3079
Fragments arranged by size:
3079 1245 988 786 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6625
HpaI GTT'AAC
Cuts at: 4215 4215
Size: 6625
KpnI G_GTAC'C
Cuts at: 2614 3950 2614
Size: 1336 5289
MfeI C'AATT_G
Cuts at: 4224 4224
Size: 6625
MluI A'CGCG_T
Cuts at: 6501 6501
Size: 6625
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2460 3456 5739 5923 1305
Size: 1155 996 2283 184 2007
Fragments arranged by size:
2283 2007 1155 996 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2375 10
Size: 54 1425 343 543 4260
Fragments arranged by size:
4260 1425 543 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2115 2327 3026 3350 4583 4942 5101
Size: 648 949 212 699 324 1233 359 159
Cuts at: 5101 518
Size: 2042
Fragments arranged by size:
2042 1233 949 699 648 359 324 212
159
MspA1I CmG'CkG
Cuts at: 1606 2250 2420 3049 4041 4718 5866 6111 1606
Size: 644 170 629 992 677 1148 245 2120
Fragments arranged by size:
2120 1148 992 677 644 629 245 170
NaeI GCC'GGC
Cuts at: 2809 2809
Size: 6625
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5134
Fragments arranged by size:
5134 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6625
NgoMIV G'CCGG_C
Cuts at: 2807 2807
Size: 6625
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6625
NotI GC'GGCC_GC
Cuts at: 4072 4072
Size: 6625
NspI r_CATG'y
Cuts at: 1132 1229 2382 3279 3370 3983 5528 1132
Size: 97 1153 897 91 613 1545 2229
Fragments arranged by size:
2229 1545 1153 897 613 97 91
PasI CC'CwG_GG
Cuts at: 1348 3637 1348
Size: 2289 4336
PciI A'CATG_T
Cuts at: 2378 5524 2378
Size: 3146 3479
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2371 3616 1108
Size: 1263 1245 4117
Fragments arranged by size:
4117 1263 1245
PfoI T'CCnGG_A
Cuts at: 2218 2988 3573 2218
Size: 770 585 5270
Fragments arranged by size:
5270 770 585
PmeI GTTT'AAAC
Cuts at: 3929 3929
Size: 6625
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4694 4726 6207 6239 4694
Size: 32 1481 32 5080
Fragments arranged by size:
5080 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3809 3809
Size: 6625
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6625
PsiI TTA'TAA
Cuts at: 4195 4195
Size: 6625
PstI C_TGCA'G
Cuts at: 830 2071 3977 830
Size: 1241 1906 3478
Fragments arranged by size:
3478 1906 1241
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6625
PvuII CAG'CTG
Cuts at: 2250 3049 2250
Size: 799 5826
SacI G_AGCT'C
Cuts at: 2227 2227
Size: 6625
SacII CC_GC'GG
Cuts at: 2421 2421
Size: 6625
SalI G'TCGA_C
Cuts at: 1936 3967 1936
Size: 2031 4594
SanDI GG'GwC_CC
Cuts at: 3809 3809
Size: 6625
SapI GCTCTTCn'nnn_
Cuts at: 3395 3395
Size: 6625
SbfI CC_TGCA'GG
Cuts at: 3977 3977
Size: 6625
ScaI AGT'ACT
Cuts at: 2552 3563 4873 6585 2552
Size: 1011 1310 1712 2592
Fragments arranged by size:
2592 1712 1310 1011
SfcI C'TryA_G
Cuts at: 826 1045 2067 3141 3320 3973 5108 5789 5980
Size: 219 1022 1074 179 653 1135 681 191
Cuts at: 5980 826
Size: 1471
Fragments arranged by size:
1471 1135 1074 1022 681 653 219 191
179
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6625
SgrAI Cr'CCGG_yG
Cuts at: 1700 2174 1700
Size: 474 6151
SmaI CCC'GGG
Cuts at: 1872 2460 3158 3952 1872
Size: 588 698 794 4545
Fragments arranged by size:
4545 794 698 588
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3844 4729 5630 5892 820
Size: 197 926 1901 885 901 262 1553
Fragments arranged by size:
1901 1553 926 901 885 262 197
SnaBI TAC'GTA
Cuts at: 493 4416 493
Size: 3923 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6625
SphI G_CATG'C
Cuts at: 1132 3279 3983 1132
Size: 2147 704 3774
Fragments arranged by size:
3774 2147 704
SspI AAT'ATT
Cuts at: 5 52 4549 5
Size: 47 4497 2081
Fragments arranged by size:
4497 2081 47
StuI AGG'CCT
Cuts at: 1517 2744 2801 1517
Size: 1227 57 5341
Fragments arranged by size:
5341 1227 57
StyI C'CwwG_G
Cuts at: 513 1065 2004 3421 4064 513
Size: 552 939 1417 643 3074
Fragments arranged by size:
3074 1417 939 643 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3124 3547 4652 4669 4822 5007 5452
Size: 1255 1544 423 1105 17 153 185 445
Cuts at: 5452 325
Size: 1498
Fragments arranged by size:
1544 1498 1255 1105 445 423 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2550 3561 4871
Size: 274 80 33 51 1443 573 1011 1310
Cuts at: 4871 6583 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1310 1011 573 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2219 2251 2876 2908 6585 6617 2219
Size: 32 625 32 3677 32 2227
Fragments arranged by size:
3677 2227 625 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3071 3439 3686 1316
Size: 1755 368 247 4255
Fragments arranged by size:
4255 1755 368 247
XbaI T'CTAG_A
Cuts at: 3961 3961
Size: 6625
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2022 2022
Size: 6625
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6625
XmaI C'CCGG_G
Cuts at: 1870 2458 3156 3950 1870
Size: 588 698 794 4545
Fragments arranged by size:
4545 794 698 588
XmnI GAAnn'nnTTC
Cuts at: 3563 4754 3563
Size: 1191 5434
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4433 276
Size: 53 83 186 936 2899 2468
Fragments arranged by size:
2899 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SacI SacII SalI
SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoNI FalI FseI FspAI KasI NarI NruI NsiI
PacI PmlI PspOMI PspXI PsrI RsrII SexAI SfiI
SfoI SrfI SwaI