GSPT1 ORF clone pFN21AE2037

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE2037

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Clone info.

Species

human

Clone Name

pFN21AE2037 Sequence (with vector) Restriction Map

Product ID

FHC10854

ORF Sequence

DNA sequence (1902 bp)
Protein sequence (634 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB590486]

Gene

KIEE2037

Symbol

GSPT1

Alias : 551G9.2, ETF3A, GST1, eRF3a

Description

G1 to S phase transition 1

Original Clone ID

cs02937

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1902 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 634 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS45412.1 5e-173 99.2 GSPT1 CCDS45413.1 2.6e-172 99.1 GSPT1 CCDS14336.1 3.1e-148 88.5 GSPT2 CCDS45414.1 3.1e-135 100.0 GSPT1 CCDS47479.1 5.6e-43 37.3 HBS1L The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR000795 211 224 PR00315 Protein synthesis factor IPR000795 270 278 PR00315 Protein synthesis factor IPR000795 290 300 PR00315 Protein synthesis factor IPR000795 306 317 PR00315 Protein synthesis factor IPR000795 350 359 PR00315 Protein synthesis factor HMMPfam IPR009818 62 78 PF07145 Ataxin-2 IPR000795 207 406 PF00009 Protein synthesis factor IPR004161 449 516 PF03144 Translation elongation factor EFTu/EF1A IPR004160 526 629 PF03143 Translation elongation factor EFTu/EF1A
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_002085 637 139259 99.4 100.0 NP_001123478 636 139259 99.2 100.0 NP_060564 628 300418 88.5 97.6 NP_001138630 642 612450 37.3 82.5 NP_006611 684 612450 37.3 82.5 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE2037 Download

>KIEE2037  1902 bp
ATGGATCCGGGCAGTGGCGGCGGCGGCGGCGGCGGCGGGAGCAGCAGCGGCAGCAGCAGC
AGCGACTCGGCGCCTGACTGCTGGGACCAGGCGGACATGGAAGCCCCCGGGCCGGGCCCT
TGCGGCGGCGGCGGCTCCCTGGCGGCGGCGGCCGAGGCCCAGCGGGAGAACCTCAGCGCG
GCCTTCAGCCGGCAACTCAACGTCAACGCCAAGCCCTTCGTGCCCAACGTCCACGCCGCC
GAGTTCGTGCCGTCCTTCCTGCGGTGCCCGGCAGCGCCGCCACCCCCAGCTGGCGGCGCC
GCCAATAACCACGGAGCCGGCAGCGGCGCGGGAGGCCGTGCGGCACCTGTGGAATCCTCT
CAAGAGGAACAGTCATTGTGTGAAGGTTCAAATTCAGCTGTTAGCATGGAACTTTCAGAA
CCTATTGTAGAAAATGGAGAGACAGAAATGTCTCCAGAAGAATCATGGGAGCACAAAGAA
GAAATAAGTGAAGCAGAGCCAGGGGGTGGTTCCTTGGGAGATGGAAGGCCGCCAGAGGAA
AGTGCCCATGAAATGATGGAGGAGGAAGAGGAAATCCCAAAACCTAAGTCTGTGGTTGCA
CCGCCAGGTGCTCCTAAGAAAGAGCATGTAAATGTAGTATTCATTGGGCACGTAGATGCT
GGCAAGTCAACCATTGGAGGACAAATAATGTATTTGACTGGAATGGTTGACAAAAGGACG
CTTGAAAAGTATGAAAGAGAAGCTAAAGAGAAAAACAGAGAAACTTGGTACTTGTCTTGG
GCCTTAGACACAAATCAGGAAGAACGAGACAAGGGTAAAACAGTAGAAGTGGGTCGTGCC
TATTTTGAAACCGAAAAGAAGCATTTCACAATTCTAGATGCCCCTGGCCACAAGAGTTTT
GTCCCAAATATGATTGGTGGTGCCTCTCAAGCTGATTTGGCTGTGCTGGTAATCTCAGCC
AGGAAAGGAGAGTTTGAAACTGGATTTGAAAAAGGAGGACAGACAAGAGAACATGCAATG
TTGGCAAAGACAGCAGGTGTAAAACACCTAATTGTGCTAATTAATAAGATGGATGATCCA
ACAGTAAATTGGAGCAATGAGAGATATGAAGAATGTAAGGAGAAACTAGTGCCATTTTTG
AAAAAAGTTGGCTTCAATCCCAAAAAGGACATTCACTTTATGCCCTGCTCAGGACTTACT
GGAGCAAATCTCAAAGAGCAGTCGGATTTCTGTCCTTGGTACATTGGATTACCGTTTATT
CCATATCTGGATAATTTGCCGAACTTCAATAGATCAGTTGATGGACCAATCAGGCTGCCA
ATTGTGGATAAGTACAAGGATATGGGCACTGTGGTCCTGGGAAAGCTGGAATCAGGATCT
ATTTGTAAAGGCCAGCAGCTTGTGATGATGCCAAACAAGCACAACGTGGAAGTTCTTGGA
ATACTTTCCGATGATGTAGAGACTGATACCGTAGCCCCAGGTGAAAACCTCAAAATCAGA
CTGAAAGGAATTGAAGAAGAGGAGATTCTTCCAGGGTTTATACTTTGTGATCCTAATAAT
CTTTGTCATTCTGGACGCACATTTGATGCCCAGATAGTGATTATAGAGCACAAATCCATC
ATCTGCCCAGGCTATAATGCGGTGCTGCATATTCATACCTGTATTGAGGAGGTGGAAATA
ACAGCCTTAATCTGCTTGGTAGACAAAAAATCAGGAGAAAAAAGTAAGACCCGACCCCGT
TTTGTCAAACAAGATCAAGTATGCATTGCTCGCTTAAGGACAGCAGGAACCATCTGCCTT
GAGACCTTTAAAGACTTCCCTCAGATGGGTCGTTTCACCTTAAGAGATGAGGGTAAGACC
ATTGCAATTGGAAAAGTTCTGAAACTGGTTCCAGAGAAAGAC

ORF Protein

Cloned ORF protein sequence for pFN21AE2037 Download

>KIEE2037  634 aa
MDPGSGGGGGGGGSSSGSSSSDSAPDCWDQADMEAPGPGPCGGGGSLAAAAEAQRENLSA
AFSRQLNVNAKPFVPNVHAAEFVPSFLRCPAAPPPPAGGAANNHGAGSGAGGRAAPVESS
QEEQSLCEGSNSAVSMELSEPIVENGETEMSPEESWEHKEEISEAEPGGGSLGDGRPPEE
SAHEMMEEEEEIPKPKSVVAPPGAPKKEHVNVVFIGHVDAGKSTIGGQIMYLTGMVDKRT
LEKYEREAKEKNRETWYLSWALDTNQEERDKGKTVEVGRAYFETEKKHFTILDAPGHKSF
VPNMIGGASQADLAVLVISARKGEFETGFEKGGQTREHAMLAKTAGVKHLIVLINKMDDP
TVNWSNERYEECKEKLVPFLKKVGFNPKKDIHFMPCSGLTGANLKEQSDFCPWYIGLPFI
PYLDNLPNFNRSVDGPIRLPIVDKYKDMGTVVLGKLESGSICKGQQLVMMPNKHNVEVLG
ILSDDVETDTVAPGENLKIRLKGIEEEEILPGFILCDPNNLCHSGRTFDAQIVIIEHKSI
ICPGYNAVLHIHTCIEEVEITALICLVDKKSGEKSKTRPRFVKQDQVCIARLRTAGTICL
ETFKDFPQMGRFTLRDEGKTIAIGKVLKLVPEKD

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE2037 Download

>pFN21AE2037  6607 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGATCCGGGCAGTGGCGGCGGCGGCGGCGGCGG
CGGGAGCAGCAGCGGCAGCAGCAGCAGCGACTCGGCGCCTGACTGCTGGGACCAGGCGGA
CATGGAAGCCCCCGGGCCGGGCCCTTGCGGCGGCGGCGGCTCCCTGGCGGCGGCGGCCGA
GGCCCAGCGGGAGAACCTCAGCGCGGCCTTCAGCCGGCAACTCAACGTCAACGCCAAGCC
CTTCGTGCCCAACGTCCACGCCGCCGAGTTCGTGCCGTCCTTCCTGCGGTGCCCGGCAGC
GCCGCCACCCCCAGCTGGCGGCGCCGCCAATAACCACGGAGCCGGCAGCGGCGCGGGAGG
CCGTGCGGCACCTGTGGAATCCTCTCAAGAGGAACAGTCATTGTGTGAAGGTTCAAATTC
AGCTGTTAGCATGGAACTTTCAGAACCTATTGTAGAAAATGGAGAGACAGAAATGTCTCC
AGAAGAATCATGGGAGCACAAAGAAGAAATAAGTGAAGCAGAGCCAGGGGGTGGTTCCTT
GGGAGATGGAAGGCCGCCAGAGGAAAGTGCCCATGAAATGATGGAGGAGGAAGAGGAAAT
CCCAAAACCTAAGTCTGTGGTTGCACCGCCAGGTGCTCCTAAGAAAGAGCATGTAAATGT
AGTATTCATTGGGCACGTAGATGCTGGCAAGTCAACCATTGGAGGACAAATAATGTATTT
GACTGGAATGGTTGACAAAAGGACGCTTGAAAAGTATGAAAGAGAAGCTAAAGAGAAAAA
CAGAGAAACTTGGTACTTGTCTTGGGCCTTAGACACAAATCAGGAAGAACGAGACAAGGG
TAAAACAGTAGAAGTGGGTCGTGCCTATTTTGAAACCGAAAAGAAGCATTTCACAATTCT
AGATGCCCCTGGCCACAAGAGTTTTGTCCCAAATATGATTGGTGGTGCCTCTCAAGCTGA
TTTGGCTGTGCTGGTAATCTCAGCCAGGAAAGGAGAGTTTGAAACTGGATTTGAAAAAGG
AGGACAGACAAGAGAACATGCAATGTTGGCAAAGACAGCAGGTGTAAAACACCTAATTGT
GCTAATTAATAAGATGGATGATCCAACAGTAAATTGGAGCAATGAGAGATATGAAGAATG
TAAGGAGAAACTAGTGCCATTTTTGAAAAAAGTTGGCTTCAATCCCAAAAAGGACATTCA
CTTTATGCCCTGCTCAGGACTTACTGGAGCAAATCTCAAAGAGCAGTCGGATTTCTGTCC
TTGGTACATTGGATTACCGTTTATTCCATATCTGGATAATTTGCCGAACTTCAATAGATC
AGTTGATGGACCAATCAGGCTGCCAATTGTGGATAAGTACAAGGATATGGGCACTGTGGT
CCTGGGAAAGCTGGAATCAGGATCTATTTGTAAAGGCCAGCAGCTTGTGATGATGCCAAA
CAAGCACAACGTGGAAGTTCTTGGAATACTTTCCGATGATGTAGAGACTGATACCGTAGC
CCCAGGTGAAAACCTCAAAATCAGACTGAAAGGAATTGAAGAAGAGGAGATTCTTCCAGG
GTTTATACTTTGTGATCCTAATAATCTTTGTCATTCTGGACGCACATTTGATGCCCAGAT
AGTGATTATAGAGCACAAATCCATCATCTGCCCAGGCTATAATGCGGTGCTGCATATTCA
TACCTGTATTGAGGAGGTGGAAATAACAGCCTTAATCTGCTTGGTAGACAAAAAATCAGG
AGAAAAAAGTAAGACCCGACCCCGTTTTGTCAAACAAGATCAAGTATGCATTGCTCGCTT
AAGGACAGCAGGAACCATCTGCCTTGAGACCTTTAAAGACTTCCCTCAGATGGGTCGTTT
CACCTTAAGAGATGAGGGTAAGACCATTGCAATTGGAAAAGTTCTGAAACTGGTTCCAGA
GAAAGACGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAG
GCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCA
CCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGA
TACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGT
GAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAAC
AACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTA
AGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTT
GCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTC
TGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAAT
GTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATG
AGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAA
CATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCAC
CCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTAC
ATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTT
CCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCC
GGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCA
CCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCC
ATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAG
GAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAA
CCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATG
GCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAA
TTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCG
GCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATT
GCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGT
CAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAG
CATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGG
CGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAG
GCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCC
GCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAG
GACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGA
CCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTC
ATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTG
TGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGT
CCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCA
GAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACA
CTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAG
TTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCA
AGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGG
GGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAA
AAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGC
CCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAA
TGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGAT
TGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCT
GAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCC
CTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATC
GAGATCT