(Circular) (Six-base) MAPSORT of: pFN21AE2037.seq Check: 7578 from: 1 to: 6607 pFN21AE2037 6607 bp With 182 enzymes: SgfI * August 16, 2010 14:46 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3029 3029 Size: 6607 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4417 278 Size: 53 83 186 936 2881 2468 Fragments arranged by size: 2881 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3705 3950 1937 Size: 1768 245 4594 Fragments arranged by size: 4594 1768 245 Acc65I G'GTAC_C Cuts at: 3928 3928 Size: 6607 AclI AA'CG_TT Cuts at: 1563 4734 5107 1563 Size: 3171 373 3063 Fragments arranged by size: 3171 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2173 4670 6054 6322 1632 Size: 541 2497 1384 268 1917 Fragments arranged by size: 2497 1917 1384 541 268 AflII C'TTAA_G Cuts at: 820 1017 3778 3844 820 Size: 197 2761 66 3583 Fragments arranged by size: 3583 2761 197 66 AflIII A'CryG_T Cuts at: 1203 5506 6483 1203 Size: 4303 977 1327 Fragments arranged by size: 4303 1327 977 AgeI A'CCGG_T Cuts at: 5438 5438 Size: 6607 AhdI GACnn_n'nnGTC Cuts at: 5336 5336 Size: 6607 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6607 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2676 1184 Size: 1492 5115 AlwNI CAG_nnn'CTG Cuts at: 4350 5922 4350 Size: 1572 5035 ApaI G_GGCC'C Cuts at: 2123 2123 Size: 6607 ApaLI G'TGCA_C Cuts at: 4664 5820 4664 Size: 1156 5451 ApoI r'AATT_y Cuts at: 1493 2395 3916 4142 4294 6403 1493 Size: 902 1521 226 152 2109 1697 Fragments arranged by size: 2109 1697 1521 902 226 152 AseI AT'TA_AT Cuts at: 160 1032 3066 5161 160 Size: 872 2034 2095 1606 Fragments arranged by size: 2095 2034 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6607 AvaI C'yCGr_G Cuts at: 1870 1943 2111 3932 1870 Size: 73 168 1821 4545 Fragments arranged by size: 4545 1821 168 73 BamHI G'GATC_C Cuts at: 2008 3937 4304 2008 Size: 1929 367 4311 Fragments arranged by size: 4311 1929 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2074 2268 2300 2347 2924 Size: 325 217 627 287 194 32 47 577 Cuts at: 2924 3928 5383 618 Size: 1004 1455 1842 Fragments arranged by size: 1842 1455 1004 627 577 325 287 217 194 47 32 BanII G_rGCy'C Cuts at: 1391 2123 3926 1391 Size: 732 1803 4072 Fragments arranged by size: 4072 1803 732 BbeI G_GCGC'C Cuts at: 2078 2304 2078 Size: 226 6381 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5636 BbvCI CC'TCA_GC Cuts at: 2177 2177 Size: 6607 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4798 4832 1903 Size: 34 2861 34 3678 Fragments arranged by size: 3678 2861 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4499 5709 4499 Size: 1210 5397 BclI T'GATC_A Cuts at: 1548 1548 Size: 6607 BfrBI ATG'CAT Cuts at: 3768 3768 Size: 6607 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2127 5218 136 Size: 107 122 71 631 605 455 3091 1525 Fragments arranged by size: 3091 1525 631 605 455 122 107 71 BglII A'GATC_T Cuts at: 6602 6602 Size: 6607 BlpI GC'TnA_GC Cuts at: 4024 4024 Size: 6607 Bme1580I G_kGCm'C Cuts at: 1790 2123 2229 2273 2551 2655 3353 4668 5824 Size: 333 106 44 278 104 698 1315 1156 Cuts at: 5824 1790 Size: 2573 Fragments arranged by size: 2573 1315 1156 698 333 278 106 104 44 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6607 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4338 5296 442 Size: 416 53 83 399 24 2921 958 1753 Fragments arranged by size: 2921 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6607 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3230 3230 Size: 6607 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2442 3225 5267 1239 Size: 1203 783 2042 2579 Fragments arranged by size: 2579 2042 1203 783 Bpu10I CC'TnA_GC Cuts at: 2177 3193 2177 Size: 1016 5591 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2349 2916 3824 4732 5597 5895 2349 Size: 567 908 908 865 298 3061 Fragments arranged by size: 3061 908 908 865 567 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3800 5270 882 Size: 617 2301 1470 2219 Fragments arranged by size: 2301 2219 1470 617 BsaAI yAC'GTr Cuts at: 493 1204 2656 4398 493 Size: 711 1452 1742 2702 Fragments arranged by size: 2702 1742 1452 711 BsaBI GATnn'nnATC Cuts at: 6601 6601 Size: 6607 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2075 2301 4414 4796 Size: 53 83 186 936 542 226 2113 382 Cuts at: 4796 5431 275 Size: 635 1451 Fragments arranged by size: 2113 1451 936 635 542 382 226 186 83 53 BsaWI w'CCGG_w Cuts at: 5040 5438 5712 5859 6270 5040 Size: 398 274 147 411 5377 Fragments arranged by size: 5377 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6382 Fragments arranged by size: 6382 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2579 3539 3686 1186 Size: 1393 960 147 4107 Fragments arranged by size: 4107 1393 960 147 BseYI C'CCAG_C Cuts at: 2085 2163 2290 5810 6306 2085 Size: 78 127 3520 496 2386 Fragments arranged by size: 3520 2386 496 127 78 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6034 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2157 4057 4818 5846 1540 Size: 275 186 156 1900 761 1028 2301 Fragments arranged by size: 2301 1900 1028 761 275 186 156 BsiHKAI G_wGCw'C Cuts at: 2478 2617 3615 4668 4753 5824 2478 Size: 139 998 1053 85 1071 3261 Fragments arranged by size: 3261 1071 1053 998 139 85 BsmI GAATG_Cn' Cuts at: 4118 4211 4118 Size: 93 6514 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2123 2229 2273 2478 2551 2617 2655 Size: 399 333 106 44 205 73 66 38 Cuts at: 2655 3353 3615 3926 4668 4753 5824 1391 Size: 698 262 311 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 698 399 333 311 262 205 106 85 73 66 44 38 BspEI T'CCGG_A Cuts at: 6270 6270 Size: 6607 BspHI T'CATG_A Cuts at: 4496 6226 4496 Size: 1730 4877 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3029 3962 844 Size: 2185 933 3489 Fragments arranged by size: 3489 2185 933 BsrBI CCG'CTC Cuts at: 1394 1823 4494 1394 Size: 429 2671 3507 Fragments arranged by size: 3507 2671 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3027 3106 3768 3864 5102 5276 65 Size: 1183 1779 79 662 96 1238 174 1396 Fragments arranged by size: 1779 1396 1238 1183 662 174 96 79 BsrFI r'CCGG_y Cuts at: 1700 1810 2193 2321 5251 5438 1700 Size: 110 383 128 2930 187 2869 Fragments arranged by size: 2930 2869 383 187 128 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6607 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6607 BssSI C'ACGA_G Cuts at: 4667 5679 4667 Size: 1012 5595 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6607 BstBI TT'CG_AA Cuts at: 5411 5411 Size: 6607 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6607 BstYI r'GATC_y Cuts at: 1972 2008 3380 3937 4304 4689 4706 6158 6244 Size: 36 1372 557 367 385 17 1452 86 Cuts at: 6244 6256 6602 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1372 557 385 367 346 86 36 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2314 513 Size: 552 939 310 4806 Fragments arranged by size: 4806 939 552 310 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5868 Fragments arranged by size: 5868 683 56 BtsI GCAGTG_nn' Cuts at: 2023 4123 4917 4937 2023 Size: 2100 794 20 3693 Fragments arranged by size: 3693 2100 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6607 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6572 DraI TTT'AAA Cuts at: 3814 3911 3814 Size: 97 6510 DraIII CAC_nnn'GTG Cuts at: 3430 3430 Size: 6607 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2452 5614 809 Size: 507 1136 3162 1802 Fragments arranged by size: 3162 1802 1136 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2154 2890 4054 Size: 54 1425 50 275 18 324 736 1164 Cuts at: 4054 4943 6572 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1164 889 736 324 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2154 4054 1537 Size: 275 342 1900 4090 Fragments arranged by size: 4090 1900 342 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2565 3516 4537 1353 Size: 1212 951 1021 3423 Fragments arranged by size: 3423 1212 1021 951 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2111 5194 5568 5714 387 Size: 1064 138 522 3083 374 146 1280 Fragments arranged by size: 3083 1280 1064 522 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2173 2442 3225 4670 5267 6054 6322 Size: 393 541 269 783 1445 597 787 268 Cuts at: 6322 1239 Size: 1524 Fragments arranged by size: 1524 1445 787 783 597 541 393 269 268 EcoO109I rG'GnC_Cy Cuts at: 2120 2120 Size: 6607 EcoRI G'AATT_C Cuts at: 3916 4294 3916 Size: 378 6229 FspI TGC'GCA Cuts at: 5113 5113 Size: 6607 HaeII r_GCGC'y Cuts at: 1678 2078 2282 2304 5754 1678 Size: 400 204 22 3450 2531 Fragments arranged by size: 3450 2531 400 204 22 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2360 2392 3064 3096 3602 Size: 32 135 32 788 32 672 32 506 Cuts at: 3602 3634 3698 3730 5248 5280 5322 5354 1373 Size: 32 64 32 1518 32 42 32 2626 Fragments arranged by size: 2626 1518 788 672 506 135 64 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2209 2673 2713 3951 4197 669 Size: 988 281 271 464 40 1238 246 3079 Fragments arranged by size: 3079 1238 988 464 281 271 246 40 HindIII A'AGCT_T Cuts at: 748 748 Size: 6607 HpaI GTT'AAC Cuts at: 4197 4197 Size: 6607 KasI G'GCGC_C Cuts at: 2074 2300 2074 Size: 226 6381 KpnI G_GTAC'C Cuts at: 3932 3932 Size: 6607 MfeI C'AATT_G Cuts at: 3324 3870 4206 3324 Size: 546 336 5725 Fragments arranged by size: 5725 546 336 MluI A'CGCG_T Cuts at: 6483 6483 Size: 6607 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3107 3207 5721 5905 1305 Size: 1802 100 2514 184 2007 Fragments arranged by size: 2514 2007 1802 184 100 MscI TGG'CCA Cuts at: 10 64 1489 1832 2892 10 Size: 54 1425 343 1060 3725 Fragments arranged by size: 3725 1425 1060 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2634 2658 4565 4924 5083 518 Size: 648 1468 24 1907 359 159 2042 Fragments arranged by size: 2042 1907 1468 648 359 159 24 MspA1I CmG'CkG Cuts at: 1606 2052 2167 2294 2328 2402 4023 4700 5848 Size: 446 115 127 34 74 1621 677 1148 Cuts at: 5848 6093 1606 Size: 245 2120 Fragments arranged by size: 2120 1621 1148 677 446 245 127 115 74 34 NaeI GCC'GGC Cuts at: 2195 2323 2195 Size: 128 6479 NarI GG'CG_CC Cuts at: 2075 2301 2075 Size: 226 6381 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5116 Fragments arranged by size: 5116 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6607 NgoMIV G'CCGG_C Cuts at: 2193 2321 2193 Size: 128 6479 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6607 NotI GC'GGCC_GC Cuts at: 4054 4054 Size: 6607 NsiI A_TGCA'T Cuts at: 3770 3770 Size: 6607 NspI r_CATG'y Cuts at: 1132 1229 2633 3020 3965 5510 1132 Size: 97 1404 387 945 1545 2229 Fragments arranged by size: 2229 1545 1404 945 387 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6607 PciI A'CATG_T Cuts at: 5506 5506 Size: 6607 PflMI CCAn_nnn'nTGG Cuts at: 1108 2510 1108 Size: 1402 5205 PmeI GTTT'AAAC Cuts at: 3911 3911 Size: 6607 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4676 4708 6189 6221 4676 Size: 32 1481 32 5062 Fragments arranged by size: 5062 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6607 PsiI TTA'TAA Cuts at: 4177 4177 Size: 6607 PspOMI G'GGCC_C Cuts at: 2119 2119 Size: 6607 PstI C_TGCA'G Cuts at: 830 3959 830 Size: 3129 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6607 PvuII CAG'CTG Cuts at: 2294 2402 2294 Size: 108 6499 SalI G'TCGA_C Cuts at: 1936 3949 1936 Size: 2013 4594 SbfI CC_TGCA'GG Cuts at: 3959 3959 Size: 6607 ScaI AGT'ACT Cuts at: 4855 6567 4855 Size: 1712 4895 SfcI C'TryA_G Cuts at: 826 1045 3955 5090 5771 5962 826 Size: 219 2910 1135 681 191 1471 Fragments arranged by size: 2910 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2076 2302 2076 Size: 226 6381 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6607 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6607 SmaI CCC'GGG Cuts at: 1872 2113 3934 1872 Size: 241 1821 4545 Fragments arranged by size: 4545 1821 241 SmlI C'TyrA_G Cuts at: 820 1017 1943 2364 2931 3778 3803 3844 4711 Size: 197 926 421 567 847 25 41 867 Cuts at: 4711 5612 5874 820 Size: 901 262 1553 Fragments arranged by size: 1553 926 901 867 847 567 421 262 197 41 25 SnaBI TAC'GTA Cuts at: 493 4398 493 Size: 3905 2702 SpeI A'CTAG_T Cuts at: 152 3130 152 Size: 2978 3629 SphI G_CATG'C Cuts at: 1132 3965 1132 Size: 2833 3774 SspI AAT'ATT Cuts at: 5 52 4531 5 Size: 47 4479 2081 Fragments arranged by size: 4479 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6607 StyI C'CwwG_G Cuts at: 513 1065 2004 2517 3239 4046 513 Size: 552 939 513 722 807 3074 Fragments arranged by size: 3074 939 807 722 552 513 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4634 4651 4804 4989 5434 325 Size: 1255 3054 17 153 185 445 1498 Fragments arranged by size: 3054 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3336 4853 6565 Size: 274 80 33 51 1443 1359 1517 1712 Cuts at: 6565 96 Size: 138 Fragments arranged by size: 1712 1517 1443 1359 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6567 6599 6567 Size: 32 6575 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6607 XbaI T'CTAG_A Cuts at: 2878 3943 2878 Size: 1065 5542 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2466 3090 2466 Size: 624 5983 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6607 XmaI C'CCGG_G Cuts at: 1870 2111 3932 1870 Size: 241 1821 4545 Fragments arranged by size: 4545 1821 241 XmnI GAAnn'nnTTC Cuts at: 2867 3448 4736 2867 Size: 581 1288 4738 Fragments arranged by size: 4738 1288 581 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4415 276 Size: 53 83 186 936 2881 2468 Fragments arranged by size: 2881 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI NruI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI