Restriction map for
pFN21AE2037
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# NO CUT ENZYMES ( 116 enzymes) #
##################################
AatII Acc65I AccIII AclI AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaLI AscI Asp718I AspEI
AspI AviII AvrII BbrPI BbsI
BcgI BciVI BclI BglII BlnI
BlpI Bpu1102I BpuAI BsaBI BsaWI
BseAI BsgI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
Eam1105I Eco47III Eco81I EcoNI EcoO65I
EcoRI EcoRV FbaI FseI FspI
HindIII HpaI KpnI KspI MaeIII
MamI MluI MroI NcoI NdeI
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfuI SgrAI SnaBI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI Tsp45I Tth111I TthHB8I
XhoI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccI 1699 GTAGAC GT^[AC][GT]AC
AcsI 390 AAATTC [GA]^AATT[CT]
ApaI 114 GGGCCC GGGCC^C
ApoI 390 AAATTC [GA]^AATT[CT]
AseI 1060 ATTAAT AT^TAAT
AsnI 1060 ATTAAT AT^TAAT
AvaI 106 CCCGGG C^[CT]CG[GA]G
BalI 885 TGGCCA TGG^CCA
BamHI 3 GGATCC G^GATCC
BanII 114 GGGCCC G[GA]GC[CT]^C
BbvCI 171 CCTCAGC CCTCAGC(-5/-2)
BglI 116 GCCCTTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 649 CACGTA [CT]AC^GT[GA]
BsaI 1801 GAGACC GGTCTC(1/5)
BsiEI 149 CGGCCG CG[GA][CT]^CG
BsoBI 106 CCCGGG C^[CT]CG[GA]G
BspMI 1033 GCAGGT ACCTGC(4/8)
BstF5I 1071 GGATG GGATG(2/0)
DraI 1807 TTTAAA TTT^AAA
DraII 114 GGGCCCT [GA]G^G[ACGT]CC[CT]
DraIII 1420 CACAACGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 441 GACAGAAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 309 CCACGG C^C[GA][CT]GG
EagI 149 CGGCCG C^GGCCG
EclXI 149 CGGCCG C^GGCCG
Eco52I 149 CGGCCG C^GGCCG
EcoO109I 114 GGGCCCT [GA]G^G[ACGT]CC[CT]
EcoT22I 1761 ATGCAT ATGCA^T
FokI 1071 GGATG GGATG(9/13)
MluNI 885 TGGCCA TGG^CCA
MscI 885 TGGCCA TGG^CCA
NsiI 1761 ATGCAT ATGCA^T
PflMI 499 CCAGGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 64 GACTC GAGTC(4/5)
PshBI 1060 ATTAAT AT^TAAT
SmaI 106 CCCGGG CCC^GGG
SpeI 1125 ACTAGT A^CTAGT
Van91I 499 CCAGGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 873 TCTAGA T^CTAGA
XmaCI 106 CCCGGG C^CCGGG
XmaI 106 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 39 enzymes) #
###################################
AccII 177 CGCG CG^CG
327 CGCG
AcyI 69 GGCGCC G[GA]^CG[CT]C
295 GGCGCC
AflII 1773 CTTAAG C^TTAAG
1839 CTTAAG
Asp700I 858 GAAGCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1439 GAATACTTTC
BbeI 69 GGCGCC GGCGC^C
295 GGCGCC
BfaI 874 CTAG C^TAG
1126 CTAG
BfrI 1773 CTTAAG C^TTAAG
1839 CTTAAG
BpmI 452 CTCCAG CTGGAG(16/14)
1199 CTGGAG
BsaHI 69 GGCGCC G[GA]^CG[CT]C
295 GGCGCC
BsmFI 83 GGGAC GGGAC(10/14)
901 GTCCC
BsrFI 188 GCCGGC [GA]^CCGG[CT]
316 GCCGGC
BstUI 177 CGCG CG^CG
327 CGCG
BstYI 3 GGATCC [GA]^GATC[CT]
1375 GGATCT
Cfr10I 188 GCCGGC [GA]^CCGG[CT]
316 GCCGGC
EaeI 149 CGGCCG [CT]^GGCC[GA]
885 TGGCCA
EarI 565 GAAGAG CTCTTC(1/4)
1516 GAAGAG
EcoT14I 512 CCTTGG C^C[AT][AT]GG
1234 CCTTGG
HgaI 717 GACGC GACGC(5/10)
1574 GACGC
Hin1I 69 GGCGCC G[GA]^CG[CT]C
295 GGCGCC
HphI 1480 GGTGA GGTGA(8/7)
1835 TCACC
KasI 69 GGCGCC G^GCGCC
295 GGCGCC
Ksp632I 565 GAAGAG CTCTTC(1/4)
1516 GAAGAG
MaeI 874 CTAG C^TAG
1126 CTAG
MfeI 1319 CAATTG C^AATTG
1865 CAATTG
MflI 3 GGATCC [GA]^GATC[CT]
1375 GGATCT
MslI 625 CATGTAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
649 CACGTAGATG
MunI 1319 CAATTG C^AATTG
1865 CAATTG
MvnI 177 CGCG CG^CG
327 CGCG
NaeI 188 GCCGGC GCC^GGC
316 GCCGGC
NarI 69 GGCGCC GG^CGCC
295 GGCGCC
NgoMIV 188 GCCGGC G^CCGGC
316 GCCGGC
NspI 624 GCATGT [GA]CATG^[CT]
1011 ACATGC
PvuII 287 CAGCTG CAG^CTG
395 CAGCTG
SfoI 69 GGCGCC GGC^GCC
295 GGCGCC
StyI 512 CCTTGG C^C[AT][AT]GG
1234 CCTTGG
TspRI 12 CAGTG CA[GC]TG[ACGT][ACGT]^
1347 CACTG
XcmI 454 CCAGAAGAATCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1078 CCAACAGTAAATTGG
XhoII 3 GGATCC [GA]^GATC[CT]
1375 GGATCT
XmnI 858 GAAGCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1439 GAATACTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaII BanI BbvI BcnI BmyI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI Bsp1286I BsrDI BsrI BssKI
BstNI Cac8I CfoI Cfr13I DdeI
DpnII EcoRII FauI Fnu4HI HaeII
HaeIII HapII HhaI HinP1I HincII
HindII HinfI HpaII ItaI MaeII
MboI MboII MnlI MseI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI SfaNI SmlI TfiI Tru9I
TseI Tsp509I