SH2B3 ORF clone pFN21AE1995

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE1995

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Clone info.

Species

human

Clone Name

pFN21AE1995 Sequence (with vector) Restriction Map

Product ID

FHC12551

ORF Sequence

DNA sequence (1725 bp)
Protein sequence (575 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE1995

Symbol

SH2B3

Alias : IDDM20, LNK

Description

SH2B adaptor protein 3, transcript variant 1

Original Clone ID

cs02895

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1725 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 575 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS9153.1 6e-138 99.5 SH2B3 CCDS76602.1 3e-77 93.1 SH2B3 CCDS78264.1 1.6e-15 35.8 SH2B2 CCDS53997.1 2.5e-14 36.8 SH2B1 CCDS53996.1 6e-14 34.7 SH2B1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR000980 364 378 PR00401 SH2 motif IPR000980 386 396 PR00401 SH2 motif IPR000980 398 409 PR00401 SH2 motif IPR000980 430 444 PR00401 SH2 motif HMMPfam IPR015012 24 80 PF08916 Phenylalanine zipper IPR001849 213 306 PF00169 Pleckstrin homology domain IPR000980 364 441 PF00017 SH2 motif HMMSmart IPR001849 195 309 SM00233 Pleckstrin homology domain IPR000980 362 447 SM00252 SH2 motif ProfileScan IPR000980 364 462 PS50001 SH2 motif
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_005466 575 133100,187950,254450,605093 99.5 100.0 XP_005253876 576 133100,187950,254450,605093 99.3 100.0 XP_005253875 615 133100,187950,254450,605093 92.9 98.6 XP_011536022 616 133100,187950,254450,605093 92.8 98.4 XP_011536021 616 133100,187950,254450,605093 92.8 98.4 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE1995 Download

>KIEE1995  1725 bp
ATGAACGGGCCTGCCCTGCAGCCCTCCTCGCCCTCTTCCGCGCCCTCAGCCTCCCCGGCG
GCGGCCCCGCGGGGCTGGAGCGAGTTCTGTGAGTTGCACGCCGTAGCGGCGGCCCGGGAG
CTGGCCCGCCAGTACTGGCTGTTCGCCCGGGAGCATCCGCAGCACGCGCCGCTGCGCGCC
GAGCTGGTGTCGCTGCAGTTCACCGACCTCTTCCAGCGCTACTTCTGCCGCGAGGTGCGC
GACGGACGGGCGCCGGGCCGCGACTACCGGGACACAGGCCGTGGGCCCCCAGCCAAGGCC
GAGGCGTCCCCGGAGCCAGGCCCCGGCCCCGCCGCCCCTGGCCTGCCCAAGGCCCGCAGC
TCTGAGGAGCTGGCCCCGCCGCGGCCGCCCGGGCCCTGCTCCTTCCAGCACTTTCGCCGC
AGCCTCCGCCACATCTTCCGCCGCCGCTCGGCCGGGGAGCTGCCAGCGGCCCACACCGCT
GCCGCCCCCGGGAGCCCCGGAGAGGCTGCTGAGACCCCCGCCCGGCCTGGCCTGGCCAAG
AAGCTCCTGCCCTGGAGCCTGGCCCGGGAGCCGCCACCCGAGGCGCTGAAGGAGGCGGTG
CTGCGCTACAGCCTGGCCGACGAGGCCTCCATGGACAGCGGGGCACGCTGGCAGCGCGGG
AGGCTGGCGCTGCGCCGGGCCCCGGGCCCCGATGGCCCCGACCGCGTGCTGGAGCTCTTC
GACCCACCCAAGAGTTCAAGGCCCAAGCTACAAGCAGCTTGCTCCAGCATCCAGGAGGTC
CGGCGGTGCACACGGCTTGAGATGCCTGACAACCTTTACACCTTTGTGCTGAAGGTGAAG
GACCGGACAGACATCATCTTTGAGGTGGGAGACGAGCAGCAGCTGAATTCATGGATGGCT
GAGCTCTCGGAGTGCACAGGCCGAGGGCTGGAGAGCACAGAAGCAGAGATGCATATTCCC
TCAGCCCTAGAGCCTAGCACGTCCAGCTCCCCAAGGGGCAGCACAGATTCCCTTAACCAA
GGTGCTTCTCCTGGGGGGCTGCTGGACCCGGCCTGCCAGAAGACGGACCATTTCCTGTCC
TGCTACCCCTGGTTCCACGGCCCCATCTCCAGAGTGAAAGCAGCTCAGCTGGTTCAGCTG
CAGGGCCCTGATGCTCATGGAGTGTTCCTGGTGCGGCAGAGCGAGACGCGGCGTGGGGAA
TACGTGCTCACTTTCAACTTTCAGGGGATAGCCAAGCACCTGCGCCTGTCGCTGACAGAG
CGGGGCCAGTGCCGTGTGCAGCACCTCCACTTTCCCTCGGTCGTGGACATGCTCCACCAC
TTCCAGCGCTCGCCCATCCCACTCGAGTGCGGCGCCGCCTGTGATGTCCGGCTCTCCAGC
TACGTGGTAGTCGTCTCCCAACCACCAGGTTCCTGCAACACGGTCCTCTTCCCTTTCTCC
CTTCCTCACTGGGATTCAGAGTCCCTTCCTCACTGGGGTTCAGAGTTGGGCCTTCCCCAC
CTTAGTTCTTCTGGCTGTCCCCGGGGGCTCAGCCCAGAGGGTCTCCCAGGGCGATCCTCA
CCCCCCGAGCAGATCTTCCACCTGGTGCCTTCGCCCGAAGAACTGGCCAACAGCCTGCAG
CACCTGGAGCATGAGCCTGTGAATCGAGCCCGGGACTCGGACTACGAAATGGACTCATCC
TCCCGGAGCCACCTGCGGGCCATAGACAATCAGTACACACCTCTC

ORF Protein

Cloned ORF protein sequence for pFN21AE1995 Download

>KIEE1995  575 aa
MNGPALQPSSPSSAPSASPAAAPRGWSEFCELHAVAAARELARQYWLFAREHPQHAPLRA
ELVSLQFTDLFQRYFCREVRDGRAPGRDYRDTGRGPPAKAEASPEPGPGPAAPGLPKARS
SEELAPPRPPGPCSFQHFRRSLRHIFRRRSAGELPAAHTAAAPGSPGEAAETPARPGLAK
KLLPWSLAREPPPEALKEAVLRYSLADEASMDSGARWQRGRLALRRAPGPDGPDRVLELF
DPPKSSRPKLQAACSSIQEVRRCTRLEMPDNLYTFVLKVKDRTDIIFEVGDEQQLNSWMA
ELSECTGRGLESTEAEMHIPSALEPSTSSSPRGSTDSLNQGASPGGLLDPACQKTDHFLS
CYPWFHGPISRVKAAQLVQLQGPDAHGVFLVRQSETRRGEYVLTFNFQGIAKHLRLSLTE
RGQCRVQHLHFPSVVDMLHHFQRSPIPLECGAACDVRLSSYVVVVSQPPGSCNTVLFPFS
LPHWDSESLPHWGSELGLPHLSSSGCPRGLSPEGLPGRSSPPEQIFHLVPSPEELANSLQ
HLEHEPVNRARDSDYEMDSSSRSHLRAIDNQYTPL

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE1995 Download

>pFN21AE1995  6430 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGAACGGGCCTGCCCTGCAGCCCTCCTCGCCCTC
TTCCGCGCCCTCAGCCTCCCCGGCGGCGGCCCCGCGGGGCTGGAGCGAGTTCTGTGAGTT
GCACGCCGTAGCGGCGGCCCGGGAGCTGGCCCGCCAGTACTGGCTGTTCGCCCGGGAGCA
TCCGCAGCACGCGCCGCTGCGCGCCGAGCTGGTGTCGCTGCAGTTCACCGACCTCTTCCA
GCGCTACTTCTGCCGCGAGGTGCGCGACGGACGGGCGCCGGGCCGCGACTACCGGGACAC
AGGCCGTGGGCCCCCAGCCAAGGCCGAGGCGTCCCCGGAGCCAGGCCCCGGCCCCGCCGC
CCCTGGCCTGCCCAAGGCCCGCAGCTCTGAGGAGCTGGCCCCGCCGCGGCCGCCCGGGCC
CTGCTCCTTCCAGCACTTTCGCCGCAGCCTCCGCCACATCTTCCGCCGCCGCTCGGCCGG
GGAGCTGCCAGCGGCCCACACCGCTGCCGCCCCCGGGAGCCCCGGAGAGGCTGCTGAGAC
CCCCGCCCGGCCTGGCCTGGCCAAGAAGCTCCTGCCCTGGAGCCTGGCCCGGGAGCCGCC
ACCCGAGGCGCTGAAGGAGGCGGTGCTGCGCTACAGCCTGGCCGACGAGGCCTCCATGGA
CAGCGGGGCACGCTGGCAGCGCGGGAGGCTGGCGCTGCGCCGGGCCCCGGGCCCCGATGG
CCCCGACCGCGTGCTGGAGCTCTTCGACCCACCCAAGAGTTCAAGGCCCAAGCTACAAGC
AGCTTGCTCCAGCATCCAGGAGGTCCGGCGGTGCACACGGCTTGAGATGCCTGACAACCT
TTACACCTTTGTGCTGAAGGTGAAGGACCGGACAGACATCATCTTTGAGGTGGGAGACGA
GCAGCAGCTGAATTCATGGATGGCTGAGCTCTCGGAGTGCACAGGCCGAGGGCTGGAGAG
CACAGAAGCAGAGATGCATATTCCCTCAGCCCTAGAGCCTAGCACGTCCAGCTCCCCAAG
GGGCAGCACAGATTCCCTTAACCAAGGTGCTTCTCCTGGGGGGCTGCTGGACCCGGCCTG
CCAGAAGACGGACCATTTCCTGTCCTGCTACCCCTGGTTCCACGGCCCCATCTCCAGAGT
GAAAGCAGCTCAGCTGGTTCAGCTGCAGGGCCCTGATGCTCATGGAGTGTTCCTGGTGCG
GCAGAGCGAGACGCGGCGTGGGGAATACGTGCTCACTTTCAACTTTCAGGGGATAGCCAA
GCACCTGCGCCTGTCGCTGACAGAGCGGGGCCAGTGCCGTGTGCAGCACCTCCACTTTCC
CTCGGTCGTGGACATGCTCCACCACTTCCAGCGCTCGCCCATCCCACTCGAGTGCGGCGC
CGCCTGTGATGTCCGGCTCTCCAGCTACGTGGTAGTCGTCTCCCAACCACCAGGTTCCTG
CAACACGGTCCTCTTCCCTTTCTCCCTTCCTCACTGGGATTCAGAGTCCCTTCCTCACTG
GGGTTCAGAGTTGGGCCTTCCCCACCTTAGTTCTTCTGGCTGTCCCCGGGGGCTCAGCCC
AGAGGGTCTCCCAGGGCGATCCTCACCCCCCGAGCAGATCTTCCACCTGGTGCCTTCGCC
CGAAGAACTGGCCAACAGCCTGCAGCACCTGGAGCATGAGCCTGTGAATCGAGCCCGGGA
CTCGGACTACGAAATGGACTCATCCTCCCGGAGCCACCTGCGGGCCATAGACAATCAGTA
CACACCTCTCGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTG
CAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTG
CCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATA
AGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATT
TGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTT
AACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTT
TTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCG
CTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGT
TTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGA
AATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTC
ATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATT
CAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCT
CACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGT
TACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGT
TTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGAC
GCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTAC
TCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCT
GCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCG
AAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGG
GAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCA
ATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAA
CAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTT
CCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATC
ATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGG
AGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATT
AAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAA
AGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAA
AAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGC
TCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGA
CAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTC
CGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTT
CTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCT
GTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTG
AGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTA
GCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCT
ACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAA
GAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTT
GCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTA
CGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTAT
CAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGAT
GGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGG
TAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGC
GATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGC
GCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCT
GCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGG
ATCGAGATCT