################################## # NO CUT ENZYMES ( 106 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AflIII AgeI AhdI AocI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbrPI BcgI BciVI BclI BfrI BlnI BsaBI BseAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrFI BsrGI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DrdI Eam1105I Eco81I EcoO65I EcoRV FbaI FseI FspI HincII HindII HindIII HpaI KpnI MaeIII MamI MfeI MluI MroI MunI NaeI NdeI NgoMIV NheI NruI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SbfI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tsp45I Tth111I Van91I XbaI XcmI XmnI ################################### # ONE CUT ENZYMES ( 29 enzymes) # ################################### AcsI 885 GAATTC [GA]^AATT[CT] AlwI 1553 GATCC GGATC(4/5) ApoI 885 GAATTC [GA]^AATT[CT] BbsI 1059 GAAGAC GAAGAC(2/6) BglII 1571 AGATCT A^GATCT BpuAI 1059 GAAGAC GAAGAC(2/6) BsaWI 842 ACCGGA [AT]^CCGG[AT] BsgI 1276 GTGCAG GTGCAG(16/14) BssHII 174 GCGCGC G^CGCGC BstXI 1376 CCAGCTACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 1571 AGATCT [GA]^GATC[CT] DraIII 1579 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG EcoNI 342 CCTGCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 885 GAATTC G^AATTC EcoT22I 949 ATGCAT ATGCA^T MflI 1571 AGATCT [GA]^GATC[CT] MseI 1013 TTAA T^TAA NcoI 629 CCATGG C^CATGG NotI 381 GCGGCCGC GC^GGCCGC NsiI 949 ATGCAT ATGCA^T NspI 1307 ACATGC [GA]CATG^[CT] PaeR7I 1342 CTCGAG C^TCGAG SapI 714 GCTCTTC GCTCTTC(1/4) ScaI 131 AGTACT AGT^ACT StuI 623 AGGCCT AGG^CCT Tru9I 1013 TTAA T^TAA Tsp509I 886 AATT ^AATT XhoI 1342 CTCGAG C^TCGAG XhoII 1571 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 36 enzymes) # ################################### AfaI 132 GTAC GT^AC 1713 GTAC Alw44I 786 GTGCAC G^TGCAC 912 GTGCAC AlwNI 877 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG 1618 CAGCACCTG Aor51HI 215 AGCGCT AGC^GCT 1325 AGCGCT ApaLI 786 GTGCAC G^TGCAC 912 GTGCAC BalI 533 TGGCCA TGG^CCA 1604 TGGCCA BbeI 249 GGCGCC GGCGC^C 1351 GGCGCC BbvCI 44 CCTCAGC CCTCAGC(-5/-2) 959 CCTCAGC BfaI 967 CTAG C^TAG 974 CTAG BsaAI 1201 TACGTG [CT]AC^GT[GA] 1381 TACGTG BsaI 511 GAGACC GGTCTC(1/5) 1540 GGTCTC BseRI 24 CTCCTC GAGGAG(10/8) 364 GAGGAG BspMI 1238 ACCTGC ACCTGC(4/8) 1691 ACCTGC BsrBI 444 CCGCTC CCGCTC(-3/-3) 1258 GAGCGG DpnII 1553 GATC ^GATC 1572 GATC EagI 382 CGGCCG C^GGCCG 449 CGGCCG EclXI 382 CGGCCG C^GGCCG 449 CGGCCG Eco47III 215 AGCGCT AGC^GCT 1325 AGCGCT Eco52I 382 CGGCCG C^GGCCG 449 CGGCCG HgaI 1185 GACGC GACGC(5/10) 304 GCGTC KasI 249 GGCGCC G^GCGCC 1351 GGCGCC KspI 67 CCGCGG CCGC^GG 379 CCGCGG MaeI 967 CTAG C^TAG 974 CTAG MboI 1553 GATC ^GATC 1572 GATC MluNI 533 TGGCCA TGG^CCA 1604 TGGCCA MscI 533 TGGCCA TGG^CCA 1604 TGGCCA MslI 819 CACCTTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1340 CACTCGAGTG NarI 249 GGCGCC GG^CGCC 1351 GGCGCC NdeII 1553 GATC ^GATC 1572 GATC RsaI 132 GTAC GT^AC 1713 GTAC SacI 712 GAGCTC GAGCT^C 901 GAGCTC SacII 67 CCGCGG CCGC^GG 379 CCGCGG Sau3AI 1553 GATC ^GATC 1572 GATC SexAI 1404 ACCAGGT A^CC[AT]GGT 1580 ACCTGGT SfoI 249 GGCGCC GGC^GCC 1351 GGCGCC SmlI 796 CTTGAG C^T[CT][GA]AG 1342 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 87 enzymes) # ######################################### AccII AciI AcyI AluI ApaI AspHI AvaI AvaII BanI BanII BbvI BcnI BglI BlpI BmyI BpmI Bpu1102I BsaHI BsaJI BsiEI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstUI Cac8I CelII CfoI Cfr13I DdeI DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NlaIII NlaIV PleI PstI PvuII Sau96I ScrFI SfaNI SfcI SfiI SmaI StyI TaqI TfiI TseI TspRI TthHB8I XmaCI XmaI