(Linear) (Six-base) MAPSORT of: KIEE1995.seq Check: 7841 from: 1 to: 1725 KIEE1995.orf With 182 enzymes: SgfI * July 3, 2012 17:06 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1247 1700 1725 Size: 1247 453 25 Fragments arranged by size: 1247 453 25 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 607 850 1725 Size: 607 243 875 Fragments arranged by size: 875 607 243 AfeI AGC'GCT Cuts at: 0 217 1327 1725 Size: 217 1110 398 Fragments arranged by size: 1110 398 217 AleI CACnn'nnGTG Cuts at: 0 823 1344 1725 Size: 823 521 381 Fragments arranged by size: 823 521 381 AlwNI CAG_nnn'CTG Cuts at: 0 882 1623 1725 Size: 882 741 102 Fragments arranged by size: 882 741 102 ApaI G_GGCC'C Cuts at: 0 287 395 681 688 1147 1725 Size: 287 108 286 7 459 578 Fragments arranged by size: 578 459 287 286 108 7 ApaLI G'TGCA_C Cuts at: 0 786 912 1725 Size: 786 126 813 Fragments arranged by size: 813 786 126 ApoI r'AATT_y Cuts at: 0 885 1725 Size: 885 840 AvaI C'yCGr_G Cuts at: 0 113 146 388 487 563 577 681 1342 Size: 113 33 242 99 76 14 104 661 Cuts at: 1342 1520 1564 1649 1725 Size: 178 44 85 76 Fragments arranged by size: 661 242 178 113 104 99 85 76 76 44 33 14 BanI G'GyrC_C Cuts at: 0 249 1351 1584 1725 Size: 249 1102 233 141 Fragments arranged by size: 1102 249 233 141 BanII G_rGCy'C Cuts at: 0 287 395 496 681 688 716 905 1147 Size: 287 108 101 185 7 28 189 242 Cuts at: 1147 1529 1650 1725 Size: 382 121 75 Fragments arranged by size: 382 287 242 189 185 121 108 101 75 28 7 BbeI G_GCGC'C Cuts at: 0 253 1355 1725 Size: 253 1102 370 Fragments arranged by size: 1102 370 253 BbsI GAAGACnn'nnnn_ Cuts at: 0 1066 1725 Size: 1066 659 BbvCI CC'TCA_GC Cuts at: 0 45 960 1725 Size: 45 915 765 Fragments arranged by size: 915 765 45 BfrBI ATG'CAT Cuts at: 0 951 1725 Size: 951 774 BglI GCCn_nnn'nGGC Cuts at: 0 55 106 390 622 692 1725 Size: 55 51 284 232 70 1033 Fragments arranged by size: 1033 284 232 70 55 51 BglII A'GATC_T Cuts at: 0 1571 1725 Size: 1571 154 BlpI GC'TnA_GC Cuts at: 0 899 1124 1528 1725 Size: 899 225 404 197 Fragments arranged by size: 899 404 225 197 Bme1580I G_kGCm'C Cuts at: 0 287 395 645 681 688 790 916 1147 Size: 287 108 250 36 7 102 126 231 Cuts at: 1147 1725 Size: 578 Fragments arranged by size: 578 287 250 231 126 108 102 36 7 BmgBI CAC'GTC Cuts at: 0 980 1725 Size: 980 745 BmrI ACTGGGnnnn_n' Cuts at: 0 1458 1482 1725 Size: 1458 24 243 Fragments arranged by size: 1458 243 24 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 96 573 730 747 949 1092 1359 1645 Size: 96 477 157 17 202 143 267 286 Cuts at: 1645 1725 Size: 80 Fragments arranged by size: 477 286 267 202 157 143 96 80 17 Bpu10I CC'TnA_GC Cuts at: 0 45 960 1725 Size: 45 915 765 Fragments arranged by size: 915 765 45 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 817 1725 Size: 817 908 BsaI GGTCTCn'nnnn_ Cuts at: 0 505 1546 1725 Size: 505 1041 179 Fragments arranged by size: 1041 505 179 BsaAI yAC'GTr Cuts at: 0 1203 1383 1725 Size: 1203 180 342 Fragments arranged by size: 1203 342 180 BsaHI Gr'CG_yC Cuts at: 0 250 304 1352 1725 Size: 250 54 1048 373 Fragments arranged by size: 1048 373 250 54 BsaWI w'CCGG_w Cuts at: 0 842 1725 Size: 842 883 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 610 640 1663 1693 1725 Size: 610 30 1023 30 32 Fragments arranged by size: 1023 610 32 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 15 379 1725 Size: 15 364 1346 Fragments arranged by size: 1346 364 15 BseYI C'CCAG_C Cuts at: 0 288 1725 Size: 288 1437 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1297 1725 Size: 1297 428 BsiEI CG_ry'CG Cuts at: 0 385 452 702 1301 1725 Size: 385 67 250 599 424 Fragments arranged by size: 599 424 385 250 67 BsiHKAI G_wGCw'C Cuts at: 0 716 790 905 916 937 1208 1725 Size: 716 74 115 11 21 271 517 Fragments arranged by size: 716 517 271 115 74 21 11 BsmBI CGTCTCn'nnnn_ Cuts at: 0 863 1177 1398 1725 Size: 863 314 221 327 Fragments arranged by size: 863 327 314 221 Bsp1286I G_dGCh'C Cuts at: 0 287 395 496 645 681 688 716 790 Size: 287 108 101 149 36 7 28 74 Cuts at: 790 905 916 937 1147 1208 1529 1650 1725 Size: 115 11 21 210 61 321 121 75 Fragments arranged by size: 321 287 210 149 121 115 108 101 75 74 61 36 28 21 11 7 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1247 1700 1725 Size: 1247 453 25 Fragments arranged by size: 1247 453 25 BsrBI CCG'CTC Cuts at: 0 446 1260 1725 Size: 446 814 465 Fragments arranged by size: 814 465 446 BssHII G'CGCG_C Cuts at: 0 174 1725 Size: 174 1551 BstXI CCAn_nnnn'nTGG Cuts at: 0 1383 1725 Size: 1383 342 BstYI r'GATC_y Cuts at: 0 1571 1725 Size: 1571 154 BtgI C'CryG_G Cuts at: 0 67 279 379 629 1095 1725 Size: 67 212 100 250 466 630 Fragments arranged by size: 630 466 250 212 100 67 DraIII CAC_nnn'GTG Cuts at: 0 1584 1725 Size: 1584 141 EaeI y'GGCC_r Cuts at: 0 382 449 533 614 1604 1725 Size: 382 67 84 81 990 121 Fragments arranged by size: 990 382 121 84 81 67 EagI C'GGCC_G Cuts at: 0 382 449 1725 Size: 382 67 1276 Fragments arranged by size: 1276 382 67 EarI CTCTTCn'nnn_ Cuts at: 0 39 214 721 1432 1725 Size: 39 175 507 711 293 Fragments arranged by size: 711 507 293 175 39 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 415 427 1725 Size: 415 12 1298 Fragments arranged by size: 1298 415 12 EcoICRI GAG'CTC Cuts at: 0 714 903 1725 Size: 714 189 822 Fragments arranged by size: 822 714 189 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 96 573 607 730 747 850 949 1092 Size: 96 477 34 123 17 103 99 143 Cuts at: 1092 1359 1645 1725 Size: 267 286 80 Fragments arranged by size: 477 286 267 143 123 103 99 96 80 34 17 EcoNI CCTnn'n_nnAGG Cuts at: 0 346 1725 Size: 346 1379 EcoO109I rG'GnC_Cy Cuts at: 0 284 319 392 678 685 1143 1144 1725 Size: 284 35 73 286 7 458 1 581 Fragments arranged by size: 581 458 286 284 73 35 7 1 EcoRI G'AATT_C Cuts at: 0 885 1725 Size: 885 840 HaeII r_GCGC'y Cuts at: 0 219 253 586 670 1329 1355 1725 Size: 219 34 333 84 659 26 370 Fragments arranged by size: 659 370 333 219 84 34 26 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 172 204 610 642 1444 1476 1663 1695 Size: 172 32 406 32 802 32 187 32 Cuts at: 1695 1725 Size: 30 Fragments arranged by size: 802 406 187 172 32 32 32 32 30 KasI G'GCGC_C Cuts at: 0 249 1351 1725 Size: 249 1102 374 Fragments arranged by size: 1102 374 249 MscI TGG'CCA Cuts at: 0 535 1606 1725 Size: 535 1071 119 Fragments arranged by size: 1071 535 119 MslI CAynn'nnrTG Cuts at: 0 823 1344 1725 Size: 823 521 381 Fragments arranged by size: 823 521 381 MspA1I CmG'CkG Cuts at: 0 69 171 381 466 478 638 882 1128 Size: 69 102 210 85 12 160 244 246 Cuts at: 1128 1137 1725 Size: 9 588 Fragments arranged by size: 588 246 244 210 160 102 85 69 12 9 NarI GG'CG_CC Cuts at: 0 250 1352 1725 Size: 250 1102 373 Fragments arranged by size: 1102 373 250 NcoI C'CATG_G Cuts at: 0 629 1725 Size: 629 1096 NotI GC'GGCC_GC Cuts at: 0 382 1725 Size: 382 1343 NsiI A_TGCA'T Cuts at: 0 953 1725 Size: 953 772 NspI r_CATG'y Cuts at: 0 1311 1725 Size: 1311 414 PasI CC'CwG_GG Cuts at: 0 1546 1725 Size: 1546 179 PfoI T'CCnGG_A Cuts at: 0 770 1681 1725 Size: 770 911 44 Fragments arranged by size: 911 770 44 PspOMI G'GGCC_C Cuts at: 0 283 391 677 684 1143 1725 Size: 283 108 286 7 459 582 Fragments arranged by size: 582 459 286 283 108 7 PspXI vC'TCGA_Gb Cuts at: 0 1342 1725 Size: 1342 383 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 124 156 1725 Size: 124 32 1569 Fragments arranged by size: 1569 124 32 PstI C_TGCA'G Cuts at: 0 20 197 1142 1619 1725 Size: 20 177 945 477 106 Fragments arranged by size: 945 477 177 106 20 PvuII CAG'CTG Cuts at: 0 882 1128 1137 1725 Size: 882 246 9 588 Fragments arranged by size: 882 588 246 9 SacI G_AGCT'C Cuts at: 0 716 905 1725 Size: 716 189 820 Fragments arranged by size: 820 716 189 SacII CC_GC'GG Cuts at: 0 70 382 1725 Size: 70 312 1343 Fragments arranged by size: 1343 312 70 SapI GCTCTTCn'nnn_ Cuts at: 0 721 1725 Size: 721 1004 ScaI AGT'ACT Cuts at: 0 133 1725 Size: 133 1592 SexAI A'CCwGG_T Cuts at: 0 1404 1580 1725 Size: 1404 176 145 Fragments arranged by size: 1404 176 145 SfcI C'TryA_G Cuts at: 0 16 193 606 1138 1615 1725 Size: 16 177 413 532 477 110 Fragments arranged by size: 532 477 413 177 110 16 SfiI GGCCn_nnn'nGGCC Cuts at: 0 390 622 692 1725 Size: 390 232 70 1033 Fragments arranged by size: 1033 390 232 70 SfoI GGC'GCC Cuts at: 0 251 1353 1725 Size: 251 1102 372 Fragments arranged by size: 1102 372 251 SmaI CCC'GGG Cuts at: 0 115 148 390 489 565 683 1522 1651 Size: 115 33 242 99 76 118 839 129 Cuts at: 1651 1725 Size: 74 Fragments arranged by size: 839 242 129 118 115 99 76 74 33 SmlI C'TyrA_G Cuts at: 0 796 1342 1725 Size: 796 546 383 Fragments arranged by size: 796 546 383 SrfI GCCC'GGGC Cuts at: 0 390 1725 Size: 390 1335 StuI AGG'CCT Cuts at: 0 625 1725 Size: 625 1100 StyI C'CwwG_G Cuts at: 0 293 347 629 991 1017 1725 Size: 293 54 282 362 26 708 Fragments arranged by size: 708 362 293 282 54 26 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 741 1287 1725 Size: 741 546 438 Fragments arranged by size: 741 546 438 TatI w'GTAC_w Cuts at: 0 131 1712 1725 Size: 131 1581 13 Fragments arranged by size: 1581 131 13 XhoI C'TCGA_G Cuts at: 0 1342 1725 Size: 1342 383 XmaI C'CCGG_G Cuts at: 0 113 146 388 487 563 681 1520 1649 Size: 113 33 242 99 76 118 839 129 Cuts at: 1649 1725 Size: 76 Fragments arranged by size: 839 242 129 118 113 99 76 76 33 Enzymes that do cut: AarI AcuI AfeI AleI AlwNI ApaI ApaLI ApoI AvaI BanI BanII BbeI BbsI BbvCI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BssHII BstXI BstYI BtgI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI HaeII Hin4I KasI MscI MslI MspA1I NarI NcoI NotI NsiI NspI PasI PfoI PspOMI PspXI PsrI PstI PvuII SacI SacII SapI ScaI SexAI SfcI SfiI SfoI SmaI SmlI SrfI StuI StyI TaqII TatI XhoI XmaI Enzymes that do not cut: AatII AccI Acc65I AclI AflII AflIII AgeI AhdI AlfI AloI AscI AseI AsiSI AvrII BaeI BamHI BcgI BciVI BclI BmtI BplI BsaBI BsiWI BsmI BspEI BspHI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DrdI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI NaeI NdeI NgoMIV NheI NruI PacI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PvuI RsrII SalI SanDI SbfI SgfI SgrAI SnaBI SpeI SphI SspI SwaI TstI Tth111I XbaI XcmI XmnI ZraI