(Circular) (Six-base) MAPSORT of: pFN21AE1995.seq Check: 4260 from: 1 to: 6430 pFN21AE1995 6430 bp With 182 enzymes: SgfI * July 6, 2012 17:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3252 3705 3252 Size: 453 5977 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4240 278 Size: 53 83 186 936 2704 2468 Fragments arranged by size: 2704 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3773 1937 Size: 1836 4594 Acc65I G'GTAC_C Cuts at: 3751 3751 Size: 6430 AclI AA'CG_TT Cuts at: 1563 4557 4930 1563 Size: 2994 373 3063 Fragments arranged by size: 3063 2994 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2612 2855 4493 5877 6145 1632 Size: 980 243 1638 1384 268 1917 Fragments arranged by size: 1917 1638 1384 980 268 243 AfeI AGC'GCT Cuts at: 2222 3332 2222 Size: 1110 5320 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6233 AflIII A'CryG_T Cuts at: 1203 5329 6306 1203 Size: 4126 977 1327 Fragments arranged by size: 4126 1327 977 AgeI A'CCGG_T Cuts at: 5261 5261 Size: 6430 AhdI GACnn_n'nnGTC Cuts at: 5159 5159 Size: 6430 AleI CACnn'nnGTG Cuts at: 1166 2828 3349 1166 Size: 1662 521 4247 Fragments arranged by size: 4247 1662 521 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6430 AlwNI CAG_nnn'CTG Cuts at: 2887 3628 4173 5745 2887 Size: 741 545 1572 3572 Fragments arranged by size: 3572 1572 741 545 ApaI G_GGCC'C Cuts at: 2292 2400 2686 2693 3152 2292 Size: 108 286 7 459 5570 Fragments arranged by size: 5570 459 286 108 7 ApaLI G'TGCA_C Cuts at: 2791 2917 4487 5643 2791 Size: 126 1570 1156 3578 Fragments arranged by size: 3578 1570 1156 126 ApoI r'AATT_y Cuts at: 1493 2890 3739 3965 4117 6226 1493 Size: 1397 849 226 152 2109 1697 Fragments arranged by size: 2109 1697 1397 849 226 152 AseI AT'TA_AT Cuts at: 160 1032 4984 160 Size: 872 3952 1606 Fragments arranged by size: 3952 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6430 AvaI C'yCGr_G Cuts at: 1870 1943 2118 2151 2393 2492 2568 2582 2686 Size: 73 175 33 242 99 76 14 104 Cuts at: 2686 3347 3525 3569 3654 3755 1870 Size: 661 178 44 85 101 4545 Fragments arranged by size: 4545 661 242 178 175 104 101 99 85 76 73 44 33 14 BamHI G'GATC_C Cuts at: 3760 4127 3760 Size: 367 6063 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2254 3356 3589 3751 5206 Size: 325 217 627 467 1102 233 162 1455 Cuts at: 5206 618 Size: 1842 Fragments arranged by size: 1842 1455 1102 627 467 325 233 217 162 BanII G_rGCy'C Cuts at: 1391 2292 2400 2501 2686 2693 2721 2910 3152 Size: 901 108 101 185 7 28 189 242 Cuts at: 3152 3534 3655 3749 1391 Size: 382 121 94 4072 Fragments arranged by size: 4072 901 382 242 189 185 121 108 101 94 28 7 BbeI G_GCGC'C Cuts at: 2258 3360 2258 Size: 1102 5328 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3071 928 Size: 971 1172 4287 Fragments arranged by size: 4287 1172 971 BbvCI CC'TCA_GC Cuts at: 2050 2965 2050 Size: 915 5515 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4621 4655 1903 Size: 34 2684 34 3678 Fragments arranged by size: 3678 2684 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4322 5532 4322 Size: 1210 5220 BclI T'GATC_A Cuts at: 1548 1548 Size: 6430 BfrBI ATG'CAT Cuts at: 2956 2956 Size: 6430 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2060 2111 2395 Size: 107 122 71 631 605 388 51 284 Cuts at: 2395 2627 2697 5041 136 Size: 232 70 2344 1525 Fragments arranged by size: 2344 1525 631 605 388 284 232 122 107 71 70 51 BglII A'GATC_T Cuts at: 3576 6425 3576 Size: 2849 3581 BlpI GC'TnA_GC Cuts at: 2904 3129 3533 3847 2904 Size: 225 404 314 5487 Fragments arranged by size: 5487 404 314 225 Bme1580I G_kGCm'C Cuts at: 1790 2292 2400 2650 2686 2693 2795 2921 3152 Size: 502 108 250 36 7 102 126 231 Cuts at: 3152 4491 5647 1790 Size: 1339 1156 2573 Fragments arranged by size: 2573 1339 1156 502 250 231 126 108 102 36 7 BmgBI CAC'GTC Cuts at: 1318 2985 1318 Size: 1667 4763 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3463 3487 4161 Size: 416 53 83 399 24 2046 24 674 Cuts at: 4161 5119 442 Size: 958 1753 Fragments arranged by size: 2046 1753 958 674 416 399 83 53 24 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6430 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2101 2578 2735 2752 2954 3097 3364 3650 Size: 862 477 157 17 202 143 267 286 Cuts at: 3650 5090 1239 Size: 1440 2579 Fragments arranged by size: 2579 1440 862 477 286 267 202 157 143 17 Bpu10I CC'TnA_GC Cuts at: 2050 2965 2050 Size: 915 5515 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2822 4555 5420 5718 2822 Size: 1733 865 298 3534 Fragments arranged by size: 3534 1733 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2510 3551 5093 882 Size: 617 1011 1041 1542 2219 Fragments arranged by size: 2219 1542 1041 1011 617 BsaAI yAC'GTr Cuts at: 493 1204 3208 3388 4221 493 Size: 711 2004 180 833 2702 Fragments arranged by size: 2702 2004 833 711 180 BsaBI GATnn'nnATC Cuts at: 6424 6424 Size: 6430 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2255 2309 3357 4237 Size: 53 83 186 936 722 54 1048 880 Cuts at: 4237 4619 5254 275 Size: 382 635 1451 Fragments arranged by size: 1451 1048 936 880 722 635 382 186 83 54 53 BsaWI w'CCGG_w Cuts at: 2847 4863 5261 5535 5682 6093 2847 Size: 2016 398 274 147 411 3184 Fragments arranged by size: 3184 2016 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2615 2645 3668 3698 1178 Size: 30 165 30 1212 30 1023 30 3910 Fragments arranged by size: 3910 1212 1023 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2020 2384 1186 Size: 834 364 5232 Fragments arranged by size: 5232 834 364 BseYI C'CCAG_C Cuts at: 2293 5633 6129 2293 Size: 3340 496 2594 Fragments arranged by size: 3340 2594 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3302 1163 Size: 573 1566 4291 Fragments arranged by size: 4291 1566 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2390 2457 2707 3306 3880 4641 Size: 275 186 389 67 250 599 574 761 Cuts at: 4641 5669 1540 Size: 1028 2301 Fragments arranged by size: 2301 1028 761 599 574 389 275 250 186 67 BsiHKAI G_wGCw'C Cuts at: 2721 2795 2910 2921 2942 3213 4491 4576 5647 Size: 74 115 11 21 271 1278 85 1071 Cuts at: 5647 2721 Size: 3504 Fragments arranged by size: 3504 1278 1071 271 115 85 74 21 11 BsmI GAATG_Cn' Cuts at: 3941 4034 3941 Size: 93 6337 BsmBI CGTCTCn'nnnn_ Cuts at: 2868 3182 3403 2868 Size: 314 221 5895 Fragments arranged by size: 5895 314 221 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2292 2400 2501 2650 2686 2693 2721 Size: 399 502 108 101 149 36 7 28 Cuts at: 2721 2795 2910 2921 2942 3152 3213 3534 3655 Size: 74 115 11 21 210 61 321 121 Cuts at: 3655 3749 4491 4576 5647 1391 Size: 94 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 502 399 321 210 149 121 115 108 101 94 85 74 61 36 28 21 11 7 BspEI T'CCGG_A Cuts at: 6093 6093 Size: 6430 BspHI T'CATG_A Cuts at: 4319 6049 4319 Size: 1730 4700 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3252 3705 3785 844 Size: 2408 453 80 3489 Fragments arranged by size: 3489 2408 453 80 BsrBI CCG'CTC Cuts at: 1394 1823 2451 3265 4317 1394 Size: 429 628 814 1052 3507 Fragments arranged by size: 3507 1052 814 628 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4925 5099 65 Size: 1183 3677 174 1396 Fragments arranged by size: 3677 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5074 5261 1700 Size: 110 3264 187 2869 Fragments arranged by size: 3264 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6430 BssHII G'CGCG_C Cuts at: 1925 2179 1925 Size: 254 6176 BssSI C'ACGA_G Cuts at: 4490 5502 4490 Size: 1012 5418 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6430 BstBI TT'CG_AA Cuts at: 5234 5234 Size: 6430 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6430 BstXI CCAn_nnnn'nTGG Cuts at: 3388 3388 Size: 6430 BstYI r'GATC_y Cuts at: 1972 3576 3760 4127 4512 4529 5981 6067 6079 Size: 1604 184 367 385 17 1452 86 12 Cuts at: 6079 6425 1972 Size: 346 1977 Fragments arranged by size: 1977 1604 1452 385 367 346 184 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2072 2284 2384 2634 3100 513 Size: 552 1007 212 100 250 466 3843 Fragments arranged by size: 3843 1007 552 466 250 212 100 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5691 Fragments arranged by size: 5691 683 56 BtsI GCAGTG_nn' Cuts at: 3946 4740 4760 3946 Size: 794 20 5616 Fragments arranged by size: 5616 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6430 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6395 DraI TTT'AAA Cuts at: 3734 3734 Size: 6430 DraIII CAC_nnn'GTG Cuts at: 3589 3589 Size: 6430 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5437 809 Size: 507 4121 1802 Fragments arranged by size: 4121 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2387 2454 2538 Size: 54 1425 50 275 18 557 67 84 Cuts at: 2538 2619 3609 3877 4766 6395 8 Size: 81 990 268 889 1629 43 Fragments arranged by size: 1629 1425 990 889 557 275 268 84 81 67 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2387 2454 3877 1537 Size: 275 575 67 1423 4090 Fragments arranged by size: 4090 1423 575 275 67 EarI CTCTTCn'nnn_ Cuts at: 1353 2044 2219 2726 3437 4360 1353 Size: 691 175 507 711 923 3423 Fragments arranged by size: 3423 923 711 691 507 175 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2420 2432 5017 5391 5537 387 Size: 1064 138 831 12 2585 374 146 1280 Fragments arranged by size: 2585 1280 1064 831 374 146 138 12 EcoICRI GAG'CTC Cuts at: 2719 2908 2719 Size: 189 6241 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2101 2578 2612 2735 2752 2855 2954 Size: 393 469 477 34 123 17 103 99 Cuts at: 2954 3097 3364 3650 4493 5090 5877 6145 1239 Size: 143 267 286 843 597 787 268 1524 Fragments arranged by size: 1524 843 787 597 477 469 393 286 268 267 143 123 103 99 34 17 EcoNI CCTnn'n_nnAGG Cuts at: 2351 2351 Size: 6430 EcoO109I rG'GnC_Cy Cuts at: 2289 2324 2397 2683 2690 3148 3149 2289 Size: 35 73 286 7 458 1 5570 Fragments arranged by size: 5570 458 286 73 35 7 1 EcoRI G'AATT_C Cuts at: 2890 3739 4117 2890 Size: 849 378 5203 Fragments arranged by size: 5203 849 378 FspI TGC'GCA Cuts at: 4936 4936 Size: 6430 HaeII r_GCGC'y Cuts at: 1678 2224 2258 2591 2675 3334 3360 5577 1678 Size: 546 34 333 84 659 26 2217 2531 Fragments arranged by size: 2531 2217 659 546 333 84 34 26 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2177 2209 2615 2647 3449 Size: 32 135 32 605 32 406 32 802 Cuts at: 3449 3481 3668 3700 5071 5103 5145 5177 1373 Size: 32 187 32 1371 32 42 32 2626 Fragments arranged by size: 2626 1371 802 605 406 187 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3774 4020 669 Size: 988 281 1836 246 3079 Fragments arranged by size: 3079 1836 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6430 HpaI GTT'AAC Cuts at: 4020 4020 Size: 6430 KasI G'GCGC_C Cuts at: 2254 3356 2254 Size: 1102 5328 KpnI G_GTAC'C Cuts at: 3755 3755 Size: 6430 MfeI C'AATT_G Cuts at: 4029 4029 Size: 6430 MluI A'CGCG_T Cuts at: 6306 6306 Size: 6430 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5544 5728 1305 Size: 4239 184 2007 Fragments arranged by size: 4239 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2540 3611 10 Size: 54 1425 343 708 1071 2829 Fragments arranged by size: 2829 1425 1071 708 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2828 3349 4388 4747 4906 518 Size: 648 1662 521 1039 359 159 2042 Fragments arranged by size: 2042 1662 1039 648 521 359 159 MspA1I CmG'CkG Cuts at: 1606 2074 2176 2386 2471 2483 2643 2887 3133 Size: 468 102 210 85 12 160 244 246 Cuts at: 3133 3142 3846 4523 5671 5916 1606 Size: 9 704 677 1148 245 2120 Fragments arranged by size: 2120 1148 704 677 468 246 245 244 210 160 102 85 12 9 NarI GG'CG_CC Cuts at: 2255 3357 2255 Size: 1102 5328 NcoI C'CATG_G Cuts at: 513 1065 2634 513 Size: 552 1569 4309 Fragments arranged by size: 4309 1569 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6430 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6430 NotI GC'GGCC_GC Cuts at: 2387 3877 2387 Size: 1490 4940 NsiI A_TGCA'T Cuts at: 2958 2958 Size: 6430 NspI r_CATG'y Cuts at: 1132 1229 3316 3788 5333 1132 Size: 97 2087 472 1545 2229 Fragments arranged by size: 2229 2087 1545 472 97 PasI CC'CwG_GG Cuts at: 1348 3551 1348 Size: 2203 4227 PciI A'CATG_T Cuts at: 5329 5329 Size: 6430 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6430 PfoI T'CCnGG_A Cuts at: 2775 3686 2775 Size: 911 5519 PmeI GTTT'AAAC Cuts at: 3734 3734 Size: 6430 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4499 4531 6012 6044 4499 Size: 32 1481 32 4885 Fragments arranged by size: 4885 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6430 PsiI TTA'TAA Cuts at: 4000 4000 Size: 6430 PspOMI G'GGCC_C Cuts at: 2288 2396 2682 2689 3148 2288 Size: 108 286 7 459 5570 Fragments arranged by size: 5570 459 286 108 7 PspXI vC'TCGA_Gb Cuts at: 3347 3347 Size: 6430 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2129 2161 2129 Size: 32 6398 PstI C_TGCA'G Cuts at: 830 2025 2202 3147 3624 3782 830 Size: 1195 177 945 477 158 3478 Fragments arranged by size: 3478 1195 945 477 177 158 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6430 PvuII CAG'CTG Cuts at: 2887 3133 3142 2887 Size: 246 9 6175 Fragments arranged by size: 6175 246 9 SacI G_AGCT'C Cuts at: 2721 2910 2721 Size: 189 6241 SacII CC_GC'GG Cuts at: 2075 2387 2075 Size: 312 6118 SalI G'TCGA_C Cuts at: 1936 3772 1936 Size: 1836 4594 SapI GCTCTTCn'nnn_ Cuts at: 2726 2726 Size: 6430 SbfI CC_TGCA'GG Cuts at: 3782 3782 Size: 6430 ScaI AGT'ACT Cuts at: 2138 4678 6390 2138 Size: 2540 1712 2178 Fragments arranged by size: 2540 2178 1712 SexAI A'CCwGG_T Cuts at: 3409 3585 3409 Size: 176 6254 SfcI C'TryA_G Cuts at: 826 1045 2021 2198 2611 3143 3620 3778 4913 Size: 219 976 177 413 532 477 158 1135 Cuts at: 4913 5594 5785 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 976 681 532 477 413 219 191 177 158 SfiI GGCCn_nnn'nGGCC Cuts at: 2395 2627 2697 2395 Size: 232 70 6128 Fragments arranged by size: 6128 232 70 SfoI GGC'GCC Cuts at: 2256 3358 2256 Size: 1102 5328 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6430 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6430 SmaI CCC'GGG Cuts at: 1872 2120 2153 2395 2494 2570 2688 3527 3656 Size: 248 33 242 99 76 118 839 129 Cuts at: 3656 3757 1872 Size: 101 4545 Fragments arranged by size: 4545 839 248 242 129 118 101 99 76 33 SmlI C'TyrA_G Cuts at: 820 1017 1943 2801 3347 4534 5435 5697 820 Size: 197 926 858 546 1187 901 262 1553 Fragments arranged by size: 1553 1187 926 901 858 546 262 197 SnaBI TAC'GTA Cuts at: 493 4221 493 Size: 3728 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6430 SphI G_CATG'C Cuts at: 1132 3788 1132 Size: 2656 3774 SrfI GCCC'GGGC Cuts at: 2395 2395 Size: 6430 SspI AAT'ATT Cuts at: 5 52 4354 5 Size: 47 4302 2081 Fragments arranged by size: 4302 2081 47 StuI AGG'CCT Cuts at: 1517 2630 1517 Size: 1113 5317 StyI C'CwwG_G Cuts at: 513 1065 2298 2352 2634 2996 3022 3869 513 Size: 552 1233 54 282 362 26 847 3074 Fragments arranged by size: 3074 1233 847 552 362 282 54 26 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2746 3292 4457 4474 4627 4812 5257 Size: 1255 1166 546 1165 17 153 185 445 Cuts at: 5257 325 Size: 1498 Fragments arranged by size: 1498 1255 1166 1165 546 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2136 3717 4676 Size: 274 80 33 51 1443 159 1581 959 Cuts at: 4676 6388 96 Size: 1712 138 Fragments arranged by size: 1712 1581 1443 959 274 159 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6390 6422 6390 Size: 32 6398 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6430 XbaI T'CTAG_A Cuts at: 3766 3766 Size: 6430 XhoI C'TCGA_G Cuts at: 1943 3347 1943 Size: 1404 5026 XmaI C'CCGG_G Cuts at: 1870 2118 2151 2393 2492 2568 2686 3525 3654 Size: 248 33 242 99 76 118 839 129 Cuts at: 3654 3755 1870 Size: 101 4545 Fragments arranged by size: 4545 839 248 242 129 118 101 99 76 33 XmnI GAAnn'nnTTC Cuts at: 4559 4559 Size: 6430 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4238 276 Size: 53 83 186 936 2704 2468 Fragments arranged by size: 2704 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BplI BsiWI BstZ17I Bsu36I EcoRV FalI FseI FspAI NaeI NgoMIV NruI PacI PmlI PpuMI RsrII SanDI SwaI XcmI