FZD4 ORF clone pFN21AE1970

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Halotag ORF Clone pFN21AE1970

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Clone info.

Species

human

Clone Name

pFN21AE1970 Sequence (with vector) Restriction Map

Product ID

FHC24400

ORF Sequence

DNA sequence (1611 bp)
Protein sequence (537 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE1970

Symbol

FZD4

Alias : CD344, EVR1, FEVR, FZD4S, Fz-4, Fz4, FzE4, GPCR, hFz4

Description

frizzled class receptor 4

Original Clone ID

cs02870

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1611 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 537 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS8279.1 0 100.0 FZD4 CCDS5548.1 2.7e-73 49.5 FZD9 CCDS9267.1 1.4e-72 51.9 FZD10 CCDS6069.1 2.5e-63 35.7 FZD3 CCDS11484.1 1.1e-62 46.1 FZD2 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR000539 222 244 PR00489 Frizzled protein IPR000539 250 272 PR00489 Frizzled protein IPR000539 304 328 PR00489 Frizzled protein IPR000539 347 370 PR00489 Frizzled protein IPR000539 392 414 PR00489 Frizzled protein IPR000539 433 454 PR00489 Frizzled protein IPR000539 476 496 PR00489 Frizzled protein HMMPfam IPR020067 45 159 PF01392 Frizzled domain IPR000539 210 512 PF01534 Frizzled protein HMMSmart IPR020067 44 163 SM00063 Frizzled domain ProfileScan IPR020067 40 161 PS50038 Frizzled domain IPR017981 219 503 PS50261 GPCR
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 15 GGVGLSLGLLLQLLLLLGPARGF 37 SECONDARY 23 2 222 WMAVWASLCFISTAFTVLTFLID 244 PRIMARY 23 3 254 PIIFLSMCYNIYSIAYIVRLTVG 276 SECONDARY 23 4 304 IIFLLMYFFGMASSIWWVILTLT 326 PRIMARY 23 5 348 HIAAWAIPAVKTIVILIMRLVDA 370 PRIMARY 23 6 391 VVAPLFTYLVIGTLFIAAGLVAL 413 PRIMARY 23 7 434 MVKIGVFSVLYTVPATCVIACYF 456 PRIMARY 23 8 472 SNMAVEMLKIFMSLLVGITSGMW 494 SECONDARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_036325 537 133780,604579 100.0 100.0 NP_003499 591 601766 49.5 97.0 NP_009128 581 606147 51.9 93.3 NP_059108 666 606143 35.7 85.7 NP_665873 666 606143 35.7 85.7 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE1970 Download

>KIEE1970  1611 bp
ATGGCCTGGCGGGGCGCAGGGCCGAGCGTCCCGGGGGCGCCCGGGGGCGTCGGTCTCAGT
CTGGGGTTGCTCCTGCAGTTGCTGCTGCTCCTGGGGCCGGCGCGGGGCTTCGGGGACGAG
GAAGAGCGGCGCTGCGACCCCATCCGCATCTCCATGTGCCAGAACCTCGGCTACAACGTG
ACCAAGATGCCCAACCTGGTTGGGCACGAGCTGCAGACGGACGCCGAGCTGCAGCTGACA
ACTTTCACACCGCTCATCCAGTACGGCTGCTCCAGCCAGCTGCAGTTCTTCCTTTGTTCT
GTTTATGTGCCAATGTGCACAGAGAAGATCAACATCCCCATTGGCCCATGCGGCGGCATG
TGTCTTTCAGTCAAGAGACGCTGTGAACCCGTCCTGAAGGAATTTGGATTTGCCTGGCCA
GAGAGTCTGAACTGCAGCAAATTCCCACCACAGAACGACCACAACCACATGTGCATGGAA
GGGCCAGGTGATGAAGAGGTGCCCTTACCTCACAAAACCCCCATCCAGCCTGGGGAAGAG
TGTCACTCTGTGGGAACCAATTCTGATCAGTACATCTGGGTGAAAAGGAGCCTGAACTGT
GTGCTCAAGTGTGGCTATGATGCTGGCTTATACAGCCGCTCAGCCAAGGAGTTCACTGAT
ATCTGGATGGCTGTGTGGGCCAGCCTGTGTTTCATCTCCACTGCCTTCACAGTACTGACC
TTCCTGATCGATTCTTCTAGGTTTTCCTACCCTGAGCGCCCCATCATATTTCTCAGTATG
TGCTATAATATTTATAGCATTGCTTATATTGTCAGGCTGACTGTAGGCCGGGAAAGGATA
TCCTGTGATTTTGAAGAGGCAGCAGAACCTGTTCTCATCCAAGAAGGACTTAAGAACACA
GGATGTGCAATAATTTTCTTGCTGATGTACTTTTTTGGAATGGCCAGCTCCATTTGGTGG
GTTATTCTGACACTCACTTGGTTTTTGGCAGCAGGACTCAAATGGGGTCATGAAGCCATT
GAAATGCACAGCTCTTATTTCCACATTGCAGCCTGGGCCATCCCCGCAGTGAAAACCATT
GTCATCTTGATTATGAGACTGGTGGATGCAGATGAACTGACTGGCTTGTGCTATGTTGGA
AACCAAAATCTCGATGCCCTCACCGGGTTCGTGGTGGCTCCCCTCTTTACTTATTTGGTC
ATTGGAACTTTGTTCATTGCTGCAGGTTTGGTGGCCTTGTTCAAAATTCGGTCAAATCTT
CAAAAGGATGGGACAAAGACAGACAAGTTAGAAAGACTGATGGTCAAGATTGGGGTGTTC
TCAGTACTGTACACAGTTCCTGCAACGTGTGTGATTGCCTGTTATTTTTATGAAATCTCC
AACTGGGCACTTTTTCGGTATTCTGCAGATGATTCCAACATGGCTGTTGAAATGTTGAAA
ATTTTTATGTCTTTGTTGGTGGGCATCACTTCAGGCATGTGGATTTGGTCTGCCAAAACT
CTTCACACGTGGCAGAAGTGTTCCAACAGATTGGTGAATTCTGGAAAGGTAAAGAGAGAG
AAGAGAGGAAATGGTTGGGTGAAGCCTGGAAAAGGCAGTGAGACTGTGGTA

ORF Protein

Cloned ORF protein sequence for pFN21AE1970 Download

>KIEE1970  537 aa
MAWRGAGPSVPGAPGGVGLSLGLLLQLLLLLGPARGFGDEEERRCDPIRISMCQNLGYNV
TKMPNLVGHELQTDAELQLTTFTPLIQYGCSSQLQFFLCSVYVPMCTEKINIPIGPCGGM
CLSVKRRCEPVLKEFGFAWPESLNCSKFPPQNDHNHMCMEGPGDEEVPLPHKTPIQPGEE
CHSVGTNSDQYIWVKRSLNCVLKCGYDAGLYSRSAKEFTDIWMAVWASLCFISTAFTVLT
FLIDSSRFSYPERPIIFLSMCYNIYSIAYIVRLTVGRERISCDFEEAAEPVLIQEGLKNT
GCAIIFLLMYFFGMASSIWWVILTLTWFLAAGLKWGHEAIEMHSSYFHIAAWAIPAVKTI
VILIMRLVDADELTGLCYVGNQNLDALTGFVVAPLFTYLVIGTLFIAAGLVALFKIRSNL
QKDGTKTDKLERLMVKIGVFSVLYTVPATCVIACYFYEISNWALFRYSADDSNMAVEMLK
IFMSLLVGITSGMWIWSAKTLHTWQKCSNRLVNSGKVKREKRGNGWVKPGKGSETVV

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE1970 Download

>pFN21AE1970  6316 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCCTGGCGGGGCGCAGGGCCGAGCGTCCCGGG
GGCGCCCGGGGGCGTCGGTCTCAGTCTGGGGTTGCTCCTGCAGTTGCTGCTGCTCCTGGG
GCCGGCGCGGGGCTTCGGGGACGAGGAAGAGCGGCGCTGCGACCCCATCCGCATCTCCAT
GTGCCAGAACCTCGGCTACAACGTGACCAAGATGCCCAACCTGGTTGGGCACGAGCTGCA
GACGGACGCCGAGCTGCAGCTGACAACTTTCACACCGCTCATCCAGTACGGCTGCTCCAG
CCAGCTGCAGTTCTTCCTTTGTTCTGTTTATGTGCCAATGTGCACAGAGAAGATCAACAT
CCCCATTGGCCCATGCGGCGGCATGTGTCTTTCAGTCAAGAGACGCTGTGAACCCGTCCT
GAAGGAATTTGGATTTGCCTGGCCAGAGAGTCTGAACTGCAGCAAATTCCCACCACAGAA
CGACCACAACCACATGTGCATGGAAGGGCCAGGTGATGAAGAGGTGCCCTTACCTCACAA
AACCCCCATCCAGCCTGGGGAAGAGTGTCACTCTGTGGGAACCAATTCTGATCAGTACAT
CTGGGTGAAAAGGAGCCTGAACTGTGTGCTCAAGTGTGGCTATGATGCTGGCTTATACAG
CCGCTCAGCCAAGGAGTTCACTGATATCTGGATGGCTGTGTGGGCCAGCCTGTGTTTCAT
CTCCACTGCCTTCACAGTACTGACCTTCCTGATCGATTCTTCTAGGTTTTCCTACCCTGA
GCGCCCCATCATATTTCTCAGTATGTGCTATAATATTTATAGCATTGCTTATATTGTCAG
GCTGACTGTAGGCCGGGAAAGGATATCCTGTGATTTTGAAGAGGCAGCAGAACCTGTTCT
CATCCAAGAAGGACTTAAGAACACAGGATGTGCAATAATTTTCTTGCTGATGTACTTTTT
TGGAATGGCCAGCTCCATTTGGTGGGTTATTCTGACACTCACTTGGTTTTTGGCAGCAGG
ACTCAAATGGGGTCATGAAGCCATTGAAATGCACAGCTCTTATTTCCACATTGCAGCCTG
GGCCATCCCCGCAGTGAAAACCATTGTCATCTTGATTATGAGACTGGTGGATGCAGATGA
ACTGACTGGCTTGTGCTATGTTGGAAACCAAAATCTCGATGCCCTCACCGGGTTCGTGGT
GGCTCCCCTCTTTACTTATTTGGTCATTGGAACTTTGTTCATTGCTGCAGGTTTGGTGGC
CTTGTTCAAAATTCGGTCAAATCTTCAAAAGGATGGGACAAAGACAGACAAGTTAGAAAG
ACTGATGGTCAAGATTGGGGTGTTCTCAGTACTGTACACAGTTCCTGCAACGTGTGTGAT
TGCCTGTTATTTTTATGAAATCTCCAACTGGGCACTTTTTCGGTATTCTGCAGATGATTC
CAACATGGCTGTTGAAATGTTGAAAATTTTTATGTCTTTGTTGGTGGGCATCACTTCAGG
CATGTGGATTTGGTCTGCCAAAACTCTTCACACGTGGCAGAAGTGTTCCAACAGATTGGT
GAATTCTGGAAAGGTAAAGAGAGAGAAGAGAGGAAATGGTTGGGTGAAGCCTGGAAAAGG
CAGTGAGACTGTGGTAGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTC
GACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGG
CTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGAC
ATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGC
TTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAA
CAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAG
GTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTC
TTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAG
CACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTT
CGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTAT
CCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATG
AGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTT
TTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGA
GTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAA
GAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGT
ATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTT
GAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGC
AGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGA
GGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGAT
CGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCT
GTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCC
CGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCG
GCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGC
GGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACG
ACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCA
CTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTC
ACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGT
GAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCC
ATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAA
ACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTC
CTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGG
CGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGC
TGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATC
GTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACA
GGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACT
ACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCG
GAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTT
TTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCT
TTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGA
GATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACG
CTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAG
TGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATC
AGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGT
GATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTA
CTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCG
TAGAGGATCGAGATCT