(Circular) (Six-base) MAPSORT of: pFN21AE1970.seq Check: 4874 from: 1 to: 6316 pFN21AE1970 6316 bp With 182 enzymes: SgfI * December 28, 2011 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4126 278 Size: 53 83 186 936 2590 2468 Fragments arranged by size: 2590 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3659 1937 Size: 1722 4594 Acc65I G'GTAC_C Cuts at: 3637 3637 Size: 6316 AclI AA'CG_TT Cuts at: 1563 4443 4816 1563 Size: 2880 373 3063 Fragments arranged by size: 3063 2880 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2420 3459 4379 5763 6031 1632 Size: 788 1039 920 1384 268 1917 Fragments arranged by size: 1917 1384 1039 920 788 268 AflII C'TTAA_G Cuts at: 820 1017 2894 820 Size: 197 1877 4242 Fragments arranged by size: 4242 1877 197 AflIII A'CryG_T Cuts at: 1203 2472 3350 3510 5215 6192 1203 Size: 1269 878 160 1705 977 1327 Fragments arranged by size: 1705 1327 1269 977 878 160 AgeI A'CCGG_T Cuts at: 5147 5147 Size: 6316 AhdI GACnn_n'nnGTC Cuts at: 3305 5045 3305 Size: 1740 4576 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6316 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6316 AlwNI CAG_nnn'CTG Cuts at: 2086 2873 3344 4059 5631 2086 Size: 787 471 715 1572 2771 Fragments arranged by size: 2771 1572 787 715 471 ApaLI G'TGCA_C Cuts at: 2320 4373 5529 2320 Size: 2053 1156 3107 Fragments arranged by size: 3107 2053 1156 ApoI r'AATT_y Cuts at: 1493 2405 2444 3249 3444 3541 3625 3851 4003 Size: 912 39 805 195 97 84 226 152 Cuts at: 4003 6112 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 912 805 226 195 152 97 84 39 AseI AT'TA_AT Cuts at: 160 1032 4870 160 Size: 872 3838 1606 Fragments arranged by size: 3838 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6316 AvaI C'yCGr_G Cuts at: 1870 1943 2035 2045 3641 1870 Size: 73 92 10 1596 4545 Fragments arranged by size: 4545 1596 92 73 10 BamHI G'GATC_C Cuts at: 3646 4013 3646 Size: 367 5949 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2041 2503 3637 5092 618 Size: 325 217 627 254 462 1134 1455 1842 Fragments arranged by size: 1842 1455 1134 627 462 325 254 217 BanII G_rGCy'C Cuts at: 1391 3635 1391 Size: 2244 4072 BbeI G_GCGC'C Cuts at: 2045 2045 Size: 6316 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5345 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4507 4541 1903 Size: 34 2570 34 3678 Fragments arranged by size: 3678 2570 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4208 5418 4208 Size: 1210 5106 BclI T'GATC_A Cuts at: 1548 2569 1548 Size: 1021 5295 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2355 4927 136 Size: 107 122 71 631 605 683 2572 1525 Fragments arranged by size: 2572 1525 683 631 605 122 107 71 BglII A'GATC_T Cuts at: 6311 6311 Size: 6316 BlpI GC'TnA_GC Cuts at: 2644 3733 2644 Size: 1089 5227 Bme1580I G_kGCm'C Cuts at: 1790 2211 2324 2508 3394 4377 5533 1790 Size: 421 113 184 886 983 1156 2573 Fragments arranged by size: 2573 1156 983 886 421 184 113 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6316 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3397 4047 5005 Size: 416 53 83 399 24 1980 650 958 Cuts at: 5005 442 Size: 1753 Fragments arranged by size: 1980 1753 958 650 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6316 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2566 2566 Size: 6316 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2260 4976 1239 Size: 1021 2716 2579 Fragments arranged by size: 2716 2579 1021 Bpu10I CC'TnA_GC Cuts at: 2757 2757 Size: 6316 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2594 4441 5306 5604 2594 Size: 1847 865 298 3306 Fragments arranged by size: 3306 1847 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2063 4979 882 Size: 617 564 2916 2219 Fragments arranged by size: 2916 2219 617 564 BsaAI yAC'GTr Cuts at: 493 1204 3513 4107 493 Size: 711 2309 594 2702 Fragments arranged by size: 2702 2309 711 594 BsaBI GATnn'nnATC Cuts at: 2336 6310 2336 Size: 3974 2342 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2042 2052 2226 4123 Size: 53 83 186 936 509 10 174 1897 Cuts at: 4123 4505 5140 275 Size: 382 635 1451 Fragments arranged by size: 1897 1451 936 635 509 382 186 174 83 53 10 BsaWI w'CCGG_w Cuts at: 4749 5147 5421 5568 5979 4749 Size: 398 274 147 411 5086 Fragments arranged by size: 5086 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2258 2288 1178 Size: 30 165 30 855 30 5206 Fragments arranged by size: 5206 855 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6316 BseYI C'CCAG_C Cuts at: 5519 6015 5519 Size: 496 5820 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5743 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3766 4527 5555 1540 Size: 275 186 1765 761 1028 2301 Fragments arranged by size: 2301 1765 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2324 2610 4377 4462 5533 2324 Size: 286 1767 85 1071 3107 Fragments arranged by size: 3107 1767 1071 286 85 BsmI GAATG_Cn' Cuts at: 3827 3920 3827 Size: 93 6223 BsmBI CGTCTCn'nnnn_ Cuts at: 2374 2374 Size: 6316 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2211 2324 2508 2610 3394 3635 4377 Size: 399 421 113 184 102 784 241 742 Cuts at: 4377 4462 5533 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 784 742 421 399 241 184 113 102 85 BspEI T'CCGG_A Cuts at: 5979 5979 Size: 6316 BspHI T'CATG_A Cuts at: 3013 4205 5935 3013 Size: 1192 1730 3394 Fragments arranged by size: 3394 1730 1192 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3218 3671 844 Size: 2374 453 3489 Fragments arranged by size: 3489 2374 453 BsrBI CCG'CTC Cuts at: 1394 1823 2131 2257 2643 4203 1394 Size: 429 308 126 386 1560 3507 Fragments arranged by size: 3507 1560 429 386 308 126 BsrDI GCAATG_nn' Cuts at: 65 1248 2802 3048 3219 4811 4985 65 Size: 1183 1554 246 171 1592 174 1396 Fragments arranged by size: 1592 1554 1396 1183 246 174 171 BsrFI r'CCGG_y Cuts at: 1700 1810 2101 4960 5147 1700 Size: 110 291 2859 187 2869 Fragments arranged by size: 2869 2859 291 187 110 BsrGI T'GTAC_A Cuts at: 96 3333 96 Size: 3237 3079 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6316 BssSI C'ACGA_G Cuts at: 2210 4376 5388 2210 Size: 2166 1012 3138 Fragments arranged by size: 3138 2166 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2086 2215 794 Size: 1292 129 4895 Fragments arranged by size: 4895 1292 129 BstBI TT'CG_AA Cuts at: 5120 5120 Size: 6316 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6316 BstXI CCAn_nnnn'nTGG Cuts at: 3535 3535 Size: 6316 BstYI r'GATC_y Cuts at: 1972 3646 4013 4398 4415 5867 5953 5965 6311 Size: 1674 367 385 17 1452 86 12 346 Cuts at: 6311 1972 Size: 1977 Fragments arranged by size: 1977 1674 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4825 Fragments arranged by size: 4825 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5577 Fragments arranged by size: 5577 683 56 BtsI GCAGTG_nn' Cuts at: 2703 3078 3607 3832 4626 4646 2703 Size: 375 529 225 794 20 4373 Fragments arranged by size: 4373 794 529 375 225 20 ClaI AT'CG_AT Cuts at: 1554 2733 1554 Size: 1179 5137 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6281 DraI TTT'AAA Cuts at: 3620 3620 Size: 6316 DraIII CAC_nnn'GTG Cuts at: 2554 2554 Size: 6316 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5323 809 Size: 507 4007 1802 Fragments arranged by size: 4007 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2420 2946 3763 Size: 54 1425 50 275 18 590 526 817 Cuts at: 3763 4652 6281 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 817 590 526 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3763 1537 Size: 275 1951 4090 Fragments arranged by size: 4090 1951 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2121 2493 2535 2853 3510 3560 4246 1353 Size: 768 372 42 318 657 50 686 3423 Fragments arranged by size: 3423 768 686 657 372 318 50 42 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4903 5277 5423 387 Size: 1064 138 3314 374 146 1280 Fragments arranged by size: 3314 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2260 2420 3459 4379 4976 5763 6031 Size: 393 628 160 1039 920 597 787 268 Cuts at: 6031 1239 Size: 1524 Fragments arranged by size: 1524 1039 920 787 628 597 393 268 160 EcoNI CCTnn'n_nnAGG Cuts at: 3594 3594 Size: 6316 EcoRI G'AATT_C Cuts at: 3541 3625 4003 3541 Size: 84 378 5854 Fragments arranged by size: 5854 378 84 EcoRV GAT'ATC Cuts at: 2665 2844 2665 Size: 179 6137 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2909 2909 Size: 6316 FspI TGC'GCA Cuts at: 4822 4822 Size: 6316 HaeII r_GCGC'y Cuts at: 1678 2045 2137 2764 5463 1678 Size: 367 92 627 2699 2531 Fragments arranged by size: 2699 2531 627 367 92 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3291 3323 4957 4989 5031 Size: 32 135 32 1719 32 1634 32 42 Cuts at: 5031 5063 1373 Size: 32 2626 Fragments arranged by size: 2626 1719 1634 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3660 3906 669 Size: 988 281 1722 246 3079 Fragments arranged by size: 3079 1722 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6316 HpaI GTT'AAC Cuts at: 3906 3906 Size: 6316 KasI G'GCGC_C Cuts at: 2041 2041 Size: 6316 KpnI G_GTAC'C Cuts at: 3641 3641 Size: 6316 MfeI C'AATT_G Cuts at: 3915 3915 Size: 6316 MluI A'CGCG_T Cuts at: 6192 6192 Size: 6316 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3121 3408 3444 3552 5430 5614 1305 Size: 1816 287 36 108 1878 184 2007 Fragments arranged by size: 2007 1878 1816 287 184 108 36 MscI TGG'CCA Cuts at: 10 64 1489 1832 2422 2948 10 Size: 54 1425 343 590 526 3378 Fragments arranged by size: 3378 1425 590 526 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2156 2477 2582 3026 4274 4633 4792 Size: 648 990 321 105 444 1248 359 159 Cuts at: 4792 518 Size: 2042 Fragments arranged by size: 2042 1248 990 648 444 359 321 159 105 MspA1I CmG'CkG Cuts at: 1606 2239 2284 3732 4409 5557 5802 1606 Size: 633 45 1448 677 1148 245 2120 Fragments arranged by size: 2120 1448 1148 677 633 245 45 NaeI GCC'GGC Cuts at: 2103 2103 Size: 6316 NarI GG'CG_CC Cuts at: 2042 2042 Size: 6316 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4825 Fragments arranged by size: 4825 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6316 NgoMIV G'CCGG_C Cuts at: 2101 2101 Size: 6316 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6316 NotI GC'GGCC_GC Cuts at: 3763 3763 Size: 6316 NspI r_CATG'y Cuts at: 1132 1229 2365 2476 3484 3674 5219 1132 Size: 97 1136 111 1008 190 1545 2229 Fragments arranged by size: 2229 1545 1136 1008 190 111 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6316 PciI A'CATG_T Cuts at: 2472 5215 2472 Size: 2743 3573 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 2961 1108 Size: 902 951 4463 Fragments arranged by size: 4463 951 902 PmeI GTTT'AAAC Cuts at: 3620 3620 Size: 6316 PmlI CAC'GTG Cuts at: 3513 3513 Size: 6316 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2862 2894 4385 4417 5898 5930 2862 Size: 32 1491 32 1481 32 3248 Fragments arranged by size: 3248 1491 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6316 PsiI TTA'TAA Cuts at: 3886 3886 Size: 6316 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2159 2191 2159 Size: 32 6284 PstI C_TGCA'G Cuts at: 830 2082 2220 2238 2289 2441 3229 3412 3668 Size: 1252 138 18 51 152 788 183 256 Cuts at: 3668 830 Size: 3478 Fragments arranged by size: 3478 1252 788 256 183 152 138 51 18 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6316 PvuII CAG'CTG Cuts at: 2239 2284 2239 Size: 45 6271 SalI G'TCGA_C Cuts at: 1936 3658 1936 Size: 1722 4594 SapI GCTCTTCn'nnn_ Cuts at: 2121 2121 Size: 6316 SbfI CC_TGCA'GG Cuts at: 3668 3668 Size: 6316 ScaI AGT'ACT Cuts at: 2718 3330 4564 6276 2718 Size: 612 1234 1712 2758 Fragments arranged by size: 2758 1712 1234 612 SexAI A'CCwGG_T Cuts at: 2199 2199 Size: 6316 SfcI C'TryA_G Cuts at: 826 1045 2078 2216 2234 2285 2437 2826 3225 Size: 219 1033 138 18 51 152 389 399 Cuts at: 3225 3408 3664 4799 5480 5671 826 Size: 183 256 1135 681 191 1471 Fragments arranged by size: 1471 1135 1033 681 399 389 256 219 191 183 152 138 51 18 SfoI GGC'GCC Cuts at: 2043 2043 Size: 6316 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6316 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6316 SmaI CCC'GGG Cuts at: 1872 2037 2047 3643 1872 Size: 165 10 1596 4545 Fragments arranged by size: 4545 1596 165 10 SmlI C'TyrA_G Cuts at: 820 1017 1943 2609 2894 4420 5321 5583 820 Size: 197 926 666 285 1526 901 262 1553 Fragments arranged by size: 1553 1526 926 901 666 285 262 197 SnaBI TAC'GTA Cuts at: 493 4107 493 Size: 3614 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6316 SphI G_CATG'C Cuts at: 1132 3674 1132 Size: 2542 3774 SspI AAT'ATT Cuts at: 5 52 2794 4240 5 Size: 47 2742 1446 2081 Fragments arranged by size: 2742 2081 1446 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6316 StyI C'CwwG_G Cuts at: 513 1065 2004 2649 3755 513 Size: 552 939 645 1106 3074 Fragments arranged by size: 3074 1106 939 645 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2045 2572 3243 3571 4343 4360 4513 Size: 1255 465 527 671 328 772 17 153 Cuts at: 4513 4698 5143 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 772 671 527 465 445 328 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2574 2716 2931 Size: 274 80 33 51 1443 597 142 215 Cuts at: 2931 3328 3333 4562 6274 96 Size: 397 5 1229 1712 138 Fragments arranged by size: 1712 1443 1229 597 397 274 215 142 138 80 51 33 5 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2244 2276 2833 2865 6276 6308 2244 Size: 32 557 32 3411 32 2252 Fragments arranged by size: 3411 2252 557 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6316 XbaI T'CTAG_A Cuts at: 3652 3652 Size: 6316 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6316 XmaI C'CCGG_G Cuts at: 1870 2035 2045 3641 1870 Size: 165 10 1596 4545 Fragments arranged by size: 4545 1596 165 10 XmnI GAAnn'nnTTC Cuts at: 2563 2874 4445 2563 Size: 311 1571 4434 Fragments arranged by size: 4434 1571 311 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4124 276 Size: 53 83 186 936 2590 2468 Fragments arranged by size: 2590 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PsrI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BsiWI BstZ17I Bsu36I EcoICRI EcoO109I FseI FspAI NruI NsiI PacI PfoI PpuMI PspOMI PspXI RsrII SacI SacII SanDI SfiI SrfI SwaI XcmI