################################## # NO CUT ENZYMES ( 105 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AgeI AlwI AocI Aor51HI ApaI AscI AseI AsnI Asp718I AspI AvaII AviII AvrII BamHI BanII BbsI BbvCI BcgI BciVI BglII BlnI BpuAI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmI BspEI BssHII Bst1107I BstBI BstEII BstPI BstYI BstZ17I Bsu36I CpoI DraI DraII DrdI DsaI EagI EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoT22I FseI FspI HincII HindII HindIII HpaI KpnI KspI MfeI MflI MluI MroI MunI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PpuMI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SbfI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I StuI SwaI Tth111I XbaI XcmI XhoI XhoII ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### AccII 101 CGCG CG^CG AflII 889 CTTAAG C^TTAAG AhdI 1295 GACTGATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 315 GTGCAC G^TGCAC ApaLI 315 GTGCAC G^TGCAC AspEI 1295 GACTGATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BbeI 36 GGCGCC GGCGC^C BbrPI 1506 CACGTG CAC^GTG BclI 564 TGATCA T^GATCA BfaI 737 CTAG C^TAG BfrI 889 CTTAAG C^TTAAG BglI 344 GCCCATGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 638 GCTCAGC GC^T[ACGT]AGC BpmI 270 CTCCAG CTGGAG(16/14) Bpu1102I 638 GCTCAGC GC^T[ACGT]AGC BsaAI 1506 CACGTG [CT]AC^GT[GA] BsaBI 327 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 52 GGTCTC GGTCTC(1/5) BsmBI 375 GAGACG CGTCTC(1/5) Bsp1407I 1328 TGTACA T^GTACA BspDI 727 ATCGAT AT^CGAT BspHI 1008 TCATGA T^CATGA BspLU11I 467 ACATGT A^CATGT BspMI 1222 GCAGGT ACCTGC(4/8) BsrFI 96 GCCGGC [GA]^CCGG[CT] BsrGI 1328 TGTACA T^GTACA BssSI 205 CACGAG CACGAG(-5/-1) BstUI 101 CGCG CG^CG BstXI 1523 CCAACAGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 638 GCTCAGC GC^T[ACGT]AGC Cfr10I 96 GCCGGC [GA]^CCGG[CT] ClaI 727 ATCGAT AT^CGAT DraIII 544 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 1295 GACTGATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 1585 CCTGGAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 1536 GAATTC G^AATTC EcoT14I 644 CCAAGG C^C[AT][AT]GG FbaI 564 TGATCA T^GATCA KasI 36 GGCGCC G^GCGCC MaeI 737 CTAG C^TAG MamI 327 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MseI 890 TTAA T^TAA MvnI 101 CGCG CG^CG NaeI 96 GCCGGC GCC^GGC NarI 36 GGCGCC GG^CGCC NgoMIV 96 GCCGGC G^CCGGC PflMI 950 CCATTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 1506 CACGTG CAC^GTG PmlI 1506 CACGTG CAC^GTG RcaI 1008 TCATGA T^CATGA SapI 121 GAAGAGC GCTCTTC(1/4) SexAI 194 ACCTGGT A^CC[AT]GGT SfoI 36 GGCGCC GGC^GCC SspBI 1328 TGTACA T^GTACA SspI 787 AATATT AAT^ATT StyI 644 CCAAGG C^C[AT][AT]GG Tru9I 890 TTAA T^TAA Van91I 950 CCATTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### Asp700I 554 GAACCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 865 GAACCTGTTC AspHI 315 GTGCAC G[AT]GC[AT]^C 601 GTGCTC AvaI 30 CCCGGG C^[CT]CG[GA]G 40 CCCGGG BalI 415 TGGCCA TGG^CCA 941 TGGCCA BanI 36 GGCGCC G^G[CT][GA]CC 498 GGTGCC BsiHKAI 315 GTGCAC G[AT]GC[AT]^C 601 GTGCTC BsoBI 30 CCCGGG C^[CT]CG[GA]G 40 CCCGGG BstAPI 75 GCAGTTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 204 GCACGAGCTGC EaeI 415 TGGCCA [CT]^GGCC[GA] 941 TGGCCA EcoRV 658 GATATC GAT^ATC 837 GATATC MaeIII 178 GTGAC ^GT[ACGT]AC 542 GTCAC MluNI 415 TGGCCA TGG^CCA 941 TGGCCA MscI 415 TGGCCA TGG^CCA 941 TGGCCA MspA1I 232 CAGCTG C[AC]G^C[GT]G 277 CAGCTG PleI 995 GACTC GAGTC(4/5) 423 GAGTC PvuII 232 CAGCTG CAG^CTG 277 CAGCTG ScaI 711 AGTACT AGT^ACT 1323 AGTACT SmaI 30 CCCGGG CCC^GGG 40 CCCGGG SmlI 604 CTCAAG C^T[CT][GA]AG 889 CTTAAG TaqI 728 TCGA T^CGA 1151 TCGA TfiI 730 GATTC G^A[AT]TC 1411 GATTC Tsp45I 178 GTGAC ^GT[GC]AC 542 GTCAC TthHB8I 728 TCGA T^CGA 1151 TCGA XmaCI 30 CCCGGG C^CCGGG 40 CCCGGG XmaI 30 CCCGGG C^CCGGG 40 CCCGGG XmnI 554 GAACCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 865 GAACCTGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 69 enzymes) # ######################################### AciI AcsI AcyI AfaI AflIII AluI AlwNI ApoI BbvI BcnI BmyI BsaHI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BsrBI BsrDI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII EarI EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MboI MboII MnlI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TseI Tsp509I TspRI