(Linear) (Six-base) MAPSORT of: KIEE1970.seq Check: 5720 from: 1 to: 1611 KIEE1970.orf With 182 enzymes: SgfI * December 27, 2011 16:31 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 415 1454 1611 Size: 415 1039 157 Fragments arranged by size: 1039 415 157 AflII C'TTAA_G Cuts at: 0 889 1611 Size: 889 722 AflIII A'CryG_T Cuts at: 0 467 1345 1505 1611 Size: 467 878 160 106 Fragments arranged by size: 878 467 160 106 AhdI GACnn_n'nnGTC Cuts at: 0 1300 1611 Size: 1300 311 AlwNI CAG_nnn'CTG Cuts at: 0 81 868 1339 1611 Size: 81 787 471 272 Fragments arranged by size: 787 471 272 81 ApaLI G'TGCA_C Cuts at: 0 315 1611 Size: 315 1296 ApoI r'AATT_y Cuts at: 0 400 439 1244 1439 1536 1611 Size: 400 39 805 195 97 75 Fragments arranged by size: 805 400 195 97 75 39 AvaI C'yCGr_G Cuts at: 0 30 40 1611 Size: 30 10 1571 Fragments arranged by size: 1571 30 10 BanI G'GyrC_C Cuts at: 0 36 498 1611 Size: 36 462 1113 Fragments arranged by size: 1113 462 36 BbeI G_GCGC'C Cuts at: 0 40 1611 Size: 40 1571 BclI T'GATC_A Cuts at: 0 564 1611 Size: 564 1047 BglI GCCn_nnn'nGGC Cuts at: 0 350 1611 Size: 350 1261 BlpI GC'TnA_GC Cuts at: 0 639 1611 Size: 639 972 Bme1580I G_kGCm'C Cuts at: 0 206 319 503 1389 1611 Size: 206 113 184 886 222 Fragments arranged by size: 886 222 206 184 113 BmrI ACTGGGnnnn_n' Cuts at: 0 1392 1611 Size: 1392 219 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 561 1611 Size: 561 1050 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 255 1611 Size: 255 1356 Bpu10I CC'TnA_GC Cuts at: 0 752 1611 Size: 752 859 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 589 1611 Size: 589 1022 BsaI GGTCTCn'nnnn_ Cuts at: 0 58 1611 Size: 58 1553 BsaAI yAC'GTr Cuts at: 0 1508 1611 Size: 1508 103 BsaBI GATnn'nnATC Cuts at: 0 331 1611 Size: 331 1280 BsaHI Gr'CG_yC Cuts at: 0 37 47 221 1611 Size: 37 10 174 1390 Fragments arranged by size: 1390 174 37 10 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 253 283 1611 Size: 253 30 1328 Fragments arranged by size: 1328 253 30 BsiHKAI G_wGCw'C Cuts at: 0 319 605 1611 Size: 319 286 1006 Fragments arranged by size: 1006 319 286 BsmBI CGTCTCn'nnnn_ Cuts at: 0 369 1611 Size: 369 1242 Bsp1286I G_dGCh'C Cuts at: 0 206 319 503 605 1389 1611 Size: 206 113 184 102 784 222 Fragments arranged by size: 784 222 206 184 113 102 BspHI T'CATG_A Cuts at: 0 1008 1611 Size: 1008 603 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1213 1611 Size: 1213 398 BsrBI CCG'CTC Cuts at: 0 126 252 638 1611 Size: 126 126 386 973 Fragments arranged by size: 973 386 126 126 BsrDI GCAATG_nn' Cuts at: 0 797 1043 1214 1611 Size: 797 246 171 397 Fragments arranged by size: 797 397 246 171 BsrFI r'CCGG_y Cuts at: 0 96 1611 Size: 96 1515 BsrGI T'GTAC_A Cuts at: 0 1328 1611 Size: 1328 283 BssSI C'ACGA_G Cuts at: 0 205 1611 Size: 205 1406 BstAPI GCAn_nnn'nTGC Cuts at: 0 81 210 1611 Size: 81 129 1401 Fragments arranged by size: 1401 129 81 BstXI CCAn_nnnn'nTGG Cuts at: 0 1530 1611 Size: 1530 81 BtsI GCAGTG_nn' Cuts at: 0 698 1073 1602 1611 Size: 698 375 529 9 Fragments arranged by size: 698 529 375 9 ClaI AT'CG_AT Cuts at: 0 728 1611 Size: 728 883 DraIII CAC_nnn'GTG Cuts at: 0 549 1611 Size: 549 1062 EaeI y'GGCC_r Cuts at: 0 415 941 1611 Size: 415 526 670 Fragments arranged by size: 670 526 415 EarI CTCTTCn'nnn_ Cuts at: 0 116 488 530 848 1505 1555 1611 Size: 116 372 42 318 657 50 56 Fragments arranged by size: 657 372 318 116 56 50 42 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 255 415 1454 1611 Size: 255 160 1039 157 Fragments arranged by size: 1039 255 160 157 EcoNI CCTnn'n_nnAGG Cuts at: 0 1589 1611 Size: 1589 22 EcoRI G'AATT_C Cuts at: 0 1536 1611 Size: 1536 75 EcoRV GAT'ATC Cuts at: 0 660 839 1611 Size: 660 179 772 Fragments arranged by size: 772 660 179 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 904 1611 Size: 904 707 HaeII r_GCGC'y Cuts at: 0 40 132 759 1611 Size: 40 92 627 852 Fragments arranged by size: 852 627 92 40 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1286 1318 1611 Size: 1286 32 293 Fragments arranged by size: 1286 293 32 KasI G'GCGC_C Cuts at: 0 36 1611 Size: 36 1575 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1116 1403 1439 1547 1611 Size: 1116 287 36 108 64 Fragments arranged by size: 1116 287 108 64 36 MscI TGG'CCA Cuts at: 0 417 943 1611 Size: 417 526 668 Fragments arranged by size: 668 526 417 MslI CAynn'nnrTG Cuts at: 0 151 472 577 1021 1611 Size: 151 321 105 444 590 Fragments arranged by size: 590 444 321 151 105 MspA1I CmG'CkG Cuts at: 0 234 279 1611 Size: 234 45 1332 Fragments arranged by size: 1332 234 45 NaeI GCC'GGC Cuts at: 0 98 1611 Size: 98 1513 NarI GG'CG_CC Cuts at: 0 37 1611 Size: 37 1574 NgoMIV G'CCGG_C Cuts at: 0 96 1611 Size: 96 1515 NspI r_CATG'y Cuts at: 0 360 471 1479 1611 Size: 360 111 1008 132 Fragments arranged by size: 1008 360 132 111 PciI A'CATG_T Cuts at: 0 467 1611 Size: 467 1144 PflMI CCAn_nnn'nTGG Cuts at: 0 956 1611 Size: 956 655 PmlI CAC'GTG Cuts at: 0 1508 1611 Size: 1508 103 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 857 889 1611 Size: 857 32 722 Fragments arranged by size: 857 722 32 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 154 186 1611 Size: 154 32 1425 Fragments arranged by size: 1425 154 32 PstI C_TGCA'G Cuts at: 0 77 215 233 284 436 1224 1407 1611 Size: 77 138 18 51 152 788 183 204 Fragments arranged by size: 788 204 183 152 138 77 51 18 PvuII CAG'CTG Cuts at: 0 234 279 1611 Size: 234 45 1332 Fragments arranged by size: 1332 234 45 SapI GCTCTTCn'nnn_ Cuts at: 0 116 1611 Size: 116 1495 ScaI AGT'ACT Cuts at: 0 713 1325 1611 Size: 713 612 286 Fragments arranged by size: 713 612 286 SexAI A'CCwGG_T Cuts at: 0 194 1611 Size: 194 1417 SfcI C'TryA_G Cuts at: 0 73 211 229 280 432 821 1220 1403 Size: 73 138 18 51 152 389 399 183 Cuts at: 1403 1611 Size: 208 Fragments arranged by size: 399 389 208 183 152 138 73 51 18 SfoI GGC'GCC Cuts at: 0 38 1611 Size: 38 1573 SmaI CCC'GGG Cuts at: 0 32 42 1611 Size: 32 10 1569 Fragments arranged by size: 1569 32 10 SmlI C'TyrA_G Cuts at: 0 604 889 1611 Size: 604 285 722 Fragments arranged by size: 722 604 285 SspI AAT'ATT Cuts at: 0 789 1611 Size: 789 822 StyI C'CwwG_G Cuts at: 0 644 1611 Size: 644 967 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 40 567 1238 1566 1611 Size: 40 527 671 328 45 Fragments arranged by size: 671 527 328 45 40 TatI w'GTAC_w Cuts at: 0 569 711 926 1323 1328 1611 Size: 569 142 215 397 5 283 Fragments arranged by size: 569 397 283 215 142 5 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 239 271 828 860 1611 Size: 239 32 557 32 751 Fragments arranged by size: 751 557 239 32 32 XmaI C'CCGG_G Cuts at: 0 30 40 1611 Size: 30 10 1571 Fragments arranged by size: 1571 30 10 XmnI GAAnn'nnTTC Cuts at: 0 558 869 1611 Size: 558 311 742 Fragments arranged by size: 742 558 311 Enzymes that do cut: AcuI AflII AflIII AhdI AlwNI ApaLI ApoI AvaI BanI BbeI BclI BglI BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaXI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstXI BtsI ClaI DraIII EaeI EarI Eco57MI EcoNI EcoRI EcoRV FalI HaeII Hin4I KasI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI PflMI PmlI PpiI PsrI PstI PvuII SapI ScaI SexAI SfcI SfoI SmaI SmlI SspI StyI TaqII TatI TstI XmaI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AgeI AleI AlfI AloI ApaI AscI AseI AsiSI AvrII BaeI BamHI BanII BbsI BbvCI BcgI BciVI BfrBI BglII BmgBI BmtI BsaWI BseRI BseYI BsgI BsiEI BsiWI BsmI BspEI BssHII BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI CspCI DraI DrdI EagI EciI EcoICRI EcoO109I FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI NcoI NdeI NheI NotI NruI NsiI PacI PasI PfoI PmeI PpuMI PshAI PsiI PspOMI PspXI PvuI RsrII SacI SacII SalI SanDI SbfI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI StuI SwaI Tth111I XbaI XcmI XhoI ZraI