ARHGAP8 ORF clone pFN21AE1927

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE1927

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Clone info.

Species

human

Clone Name

pFN21AE1927 Sequence (with vector) Restriction Map

Product ID

FHC27261

ORF Sequence

DNA sequence (1299 bp)
Protein sequence (433 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB590903]

Gene

KIEE1927

Symbol

ARHGAP8

Alias : BPGAP1, PP610

Description

Rho GTPase activating protein 8, transcript variant 2

Original Clone ID

cs02827

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1299 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 433 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS14060.2 1.1e-141 99.8 ARHGAP8 CCDS54538.2 1.4e-141 99.8 ARHGAP8 CCDS33664.1 7.3e-107 93.1 ARHGAP8 CCDS56233.1 7.3e-96 100.0 ARHGAP8 CCDS7922.1 5.7e-70 57.1 ARHGAP1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR001251 70 154 PF00650 Cellular retinaldehyde-binding/triple function IPR000198 210 356 PF00620 Rho GTPase-activating protein domain HMMSmart IPR001251 14 165 SM00516 Cellular retinaldehyde-binding/triple function IPR000198 207 378 SM00324 Rho GTPase-activating protein domain ProfileScan IPR001251 13 168 PS50191 Cellular retinaldehyde-binding/triple function IPR000198 195 381 PS50238 Rho GTPase-activating protein domain
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_851852 433 609405 99.8 100.0 NP_001017526 464 609405 93.1 100.0 XP_011518397 439 602732 57.1 88.0 NP_004299 439 602732 57.1 88.0 XP_016873209 395 602732 49.0 88.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE1927 Download

>KIEE1927  1299 bp
ATGGCTGGCCAGGATCCTGCGCTGAGCACGAGTCACCCGTTCTACGACGTGGCCAGACAT
GGCATTCTGCAGGTGGCAGGGGATGACCGCTTTGGAAGACGTGTTGTCACGTTCAGCTGC
TGCCGGATGCCACCCTCCCACGAGCTGGACCACCAGCGGCTGCTGGAGTATTTGAAGTAC
ACACTGGACCAATACGTTGAGAACGATTATACCATCGTCTATTTCCACTACGGGCTGAAC
AGCCGGAACAAGCCTTCCCTGGGCTGGCTCCAGAGCGCATACAAGGAGTTCGATAGGAAG
TACAAGAAGAACTTGAAGGCCCTCTACGTGGTGCACCCCACCAGCTTCATCAAGGTCCTG
TGGAACATCTTGAAGCCCCTCATCAGTCACAAGTTTGGGAAGAAAGTCATCTATTTCAAC
TACCTGAGTGAGCTCCACGAACACCTTAAATACGACCAGCTGGTCATCCCTCCCGAAGTT
TTGCGGTACGATGAGAAGCTCCAGAGCCTGCACGAGGGCCGGACGCCGCCTCCCACCAAG
ACACCACCGCCGCGGCCCCCGCTGCCCACACAGCAGTTTGGCGTCAGTCTGCAATACCTC
AAAGACAAAAATCAAGGCGAACTCATCCCCCCTGTGCTGAGGTTCACAGTGACGTACCTG
AGAGAGAAAGGCCTGCGCACCGAGGGCCTGTTCCGGAGATCCGCCAGCGTGCAGACCGTC
CGCGAGATCCAGAGGCTCTACAACCAAGGGAAGCCCGTGAACTTTGACGACTACGGGGAC
ATTCACATCCCTGCCGTGATCCTGAAGACCTTCCTGCGAGAGCTGCCCCAGCCGCTTCTG
ACCTTCCAGGCCTACGAGCAGATTCTCGGGATCACCTGTGTGGAGAGCAGCCTGCGTGTC
ACTGGCTGCCGCCAGATCTTACGGAGCCTCCCAGAGCACAACTACGTCGTCCTCCGCTAC
CTCATGGGCTTCCTGCATGCGGTGTCCCGGGAGAGCATCTTCAACAAAATGAACAGCTCT
AACCTGGCCTGTGTCTTCGGGCTGAATTTGATCTGGCCATCCCAGGGGGTCTCCTCCCTG
AGTGCCCTTGTGCCCCTGAACATGTTCACTGAACTGCTGATCGAGTACTATGAAAAGATC
TTCAGCACCCTGGAGGCACCTGGGGAGCACGGCCTGGCACCATGGGAACAGGGGAGCAGG
GCAGCCCCTTTGCAGGAGGCTGTGCCACGGACACAAGCCACGGGCCTCACCAAGCCTACC
CTACCTCCGAGTCCCCTGATGGCAGCCAGAAGACGTCTC

ORF Protein

Cloned ORF protein sequence for pFN21AE1927 Download

>KIEE1927  433 aa
MAGQDPALSTSHPFYDVARHGILQVAGDDRFGRRVVTFSCCRMPPSHELDHQRLLEYLKY
TLDQYVENDYTIVYFHYGLNSRNKPSLGWLQSAYKEFDRKYKKNLKALYVVHPTSFIKVL
WNILKPLISHKFGKKVIYFNYLSELHEHLKYDQLVIPPEVLRYDEKLQSLHEGRTPPPTK
TPPPRPPLPTQQFGVSLQYLKDKNQGELIPPVLRFTVTYLREKGLRTEGLFRRSASVQTV
REIQRLYNQGKPVNFDDYGDIHIPAVILKTFLRELPQPLLTFQAYEQILGITCVESSLRV
TGCRQILRSLPEHNYVVLRYLMGFLHAVSRESIFNKMNSSNLACVFGLNLIWPSQGVSSL
SALVPLNMFTELLIEYYEKIFSTLEAPGEHGLAPWEQGSRAAPLQEAVPRTQATGLTKPT
LPPSPLMAARRRL

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE1927 Download

>pFN21AE1927  6004 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCTGGCCAGGATCCTGCGCTGAGCACGAGTCA
CCCGTTCTACGACGTGGCCAGACATGGCATTCTGCAGGTGGCAGGGGATGACCGCTTTGG
AAGACGTGTTGTCACGTTCAGCTGCTGCCGGATGCCACCCTCCCACGAGCTGGACCACCA
GCGGCTGCTGGAGTATTTGAAGTACACACTGGACCAATACGTTGAGAACGATTATACCAT
CGTCTATTTCCACTACGGGCTGAACAGCCGGAACAAGCCTTCCCTGGGCTGGCTCCAGAG
CGCATACAAGGAGTTCGATAGGAAGTACAAGAAGAACTTGAAGGCCCTCTACGTGGTGCA
CCCCACCAGCTTCATCAAGGTCCTGTGGAACATCTTGAAGCCCCTCATCAGTCACAAGTT
TGGGAAGAAAGTCATCTATTTCAACTACCTGAGTGAGCTCCACGAACACCTTAAATACGA
CCAGCTGGTCATCCCTCCCGAAGTTTTGCGGTACGATGAGAAGCTCCAGAGCCTGCACGA
GGGCCGGACGCCGCCTCCCACCAAGACACCACCGCCGCGGCCCCCGCTGCCCACACAGCA
GTTTGGCGTCAGTCTGCAATACCTCAAAGACAAAAATCAAGGCGAACTCATCCCCCCTGT
GCTGAGGTTCACAGTGACGTACCTGAGAGAGAAAGGCCTGCGCACCGAGGGCCTGTTCCG
GAGATCCGCCAGCGTGCAGACCGTCCGCGAGATCCAGAGGCTCTACAACCAAGGGAAGCC
CGTGAACTTTGACGACTACGGGGACATTCACATCCCTGCCGTGATCCTGAAGACCTTCCT
GCGAGAGCTGCCCCAGCCGCTTCTGACCTTCCAGGCCTACGAGCAGATTCTCGGGATCAC
CTGTGTGGAGAGCAGCCTGCGTGTCACTGGCTGCCGCCAGATCTTACGGAGCCTCCCAGA
GCACAACTACGTCGTCCTCCGCTACCTCATGGGCTTCCTGCATGCGGTGTCCCGGGAGAG
CATCTTCAACAAAATGAACAGCTCTAACCTGGCCTGTGTCTTCGGGCTGAATTTGATCTG
GCCATCCCAGGGGGTCTCCTCCCTGAGTGCCCTTGTGCCCCTGAACATGTTCACTGAACT
GCTGATCGAGTACTATGAAAAGATCTTCAGCACCCTGGAGGCACCTGGGGAGCACGGCCT
GGCACCATGGGAACAGGGGAGCAGGGCAGCCCCTTTGCAGGAGGCTGTGCCACGGACACA
AGCCACGGGCCTCACCAAGCCTACCCTACCTCCGAGTCCCCTGATGGCAGCCAGAAGACG
TCTCGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCA
TGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCG
CTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATAC
ATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAA
ATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAAC
AACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGC
AAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCG
TTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGC
GGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTG
CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGA
CAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACAT
TTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCA
GAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATC
GAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCA
ATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGG
CAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCA
GTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATA
ACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAG
CTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCG
GAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCA
ACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTA
ATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCT
GGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCA
GCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAG
GCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCAT
TGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGG
TAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCC
AGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCC
CCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGAC
TATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCC
TGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATA
GCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGC
ACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCA
ACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAG
CGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTA
GAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTG
GTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGC
AGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGT
CTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAA
GGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCT
TCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGT
GGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGC
GGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAA
CGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTC
GTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAG
ATCT