(Circular) (Six-base) MAPSORT of: pFN21AE1927.seq Check: 6019 from: 1 to: 6004 pFN21AE1927 6004 bp With 182 enzymes: SgfI * August 16, 2010 14:52 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2065 2065 Size: 6004 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3301 3814 278 Size: 53 83 186 936 1765 513 2468 Fragments arranged by size: 2468 1765 936 513 186 83 53 AccI GT'mk_AC Cuts at: 1937 3347 1937 Size: 1410 4594 Acc65I G'GTAC_C Cuts at: 3325 3325 Size: 6004 AclI AA'CG_TT Cuts at: 1563 4131 4504 1563 Size: 2568 373 3063 Fragments arranged by size: 3063 2568 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2828 3130 4067 5451 5719 1632 Size: 1196 302 937 1384 268 1917 Fragments arranged by size: 1917 1384 1196 937 302 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5807 AflIII A'CryG_T Cuts at: 1203 2104 3105 4903 5880 1203 Size: 901 1001 1798 977 1327 Fragments arranged by size: 1798 1327 1001 977 901 AgeI A'CCGG_T Cuts at: 4835 4835 Size: 6004 AhdI GACnn_n'nnGTC Cuts at: 2108 4733 2108 Size: 2625 3379 AleI CACnn'nnGTG Cuts at: 1166 2882 1166 Size: 1716 4288 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6004 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2243 2275 2243 Size: 32 5972 AlwNI CAG_nnn'CTG Cuts at: 2124 2164 2512 3747 5319 2124 Size: 40 348 1235 1572 2809 Fragments arranged by size: 2809 1572 1235 348 40 ApaLI G'TGCA_C Cuts at: 2336 4061 5217 2336 Size: 1725 1156 3123 Fragments arranged by size: 3123 1725 1156 ApoI r'AATT_y Cuts at: 1493 3049 3313 3539 3691 5800 1493 Size: 1556 264 226 152 2109 1697 Fragments arranged by size: 2109 1697 1556 264 226 152 AseI AT'TA_AT Cuts at: 160 1032 4558 160 Size: 872 3526 1606 Fragments arranged by size: 3526 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6004 AvaI C'yCGr_G Cuts at: 1870 1943 2870 2991 3329 1870 Size: 73 927 121 338 4545 Fragments arranged by size: 4545 927 338 121 73 BamHI G'GATC_C Cuts at: 2017 3334 3701 2017 Size: 1317 367 4320 Fragments arranged by size: 4320 1317 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3160 3181 3325 4780 618 Size: 325 217 627 1373 21 144 1455 1842 Fragments arranged by size: 1842 1455 1373 627 325 217 144 21 BanII G_rGCy'C Cuts at: 1391 2439 3323 1391 Size: 1048 884 4072 Fragments arranged by size: 4072 1048 884 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2107 2816 3031 3301 928 Size: 971 208 709 215 270 3631 Fragments arranged by size: 3631 971 709 270 215 208 BbvCI CC'TCA_GC Cuts at: 2642 2642 Size: 6004 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4195 4229 1903 Size: 34 2258 34 3678 Fragments arranged by size: 3678 2258 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3896 5106 3896 Size: 1210 4794 BclI T'GATC_A Cuts at: 1548 1548 Size: 6004 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4615 136 Size: 107 122 71 631 605 2943 1525 Fragments arranged by size: 2943 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2919 3141 5999 2919 Size: 222 2858 2924 Fragments arranged by size: 2924 2858 222 BlpI GC'TnA_GC Cuts at: 2027 3421 2027 Size: 1394 4610 Bme1580I G_kGCm'C Cuts at: 1790 2340 3091 3099 4065 5221 1790 Size: 550 751 8 966 1156 2573 Fragments arranged by size: 2573 1156 966 751 550 8 BmgBI CAC'GTC Cuts at: 1318 2053 2105 1318 Size: 735 52 5217 Fragments arranged by size: 5217 735 52 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3735 4693 442 Size: 416 53 83 399 24 2318 958 1753 Fragments arranged by size: 2318 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6004 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2189 2258 2489 3176 4664 1239 Size: 950 69 231 687 1488 2579 Fragments arranged by size: 2579 1488 950 687 231 69 Bpu10I CC'TnA_GC Cuts at: 2642 2642 Size: 6004 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4129 4994 5292 4129 Size: 865 298 4841 Fragments arranged by size: 4841 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3079 4667 882 Size: 617 1580 1588 2219 Fragments arranged by size: 2219 1588 1580 617 BsaAI yAC'GTr Cuts at: 493 1204 2332 3795 493 Size: 711 1128 1463 2702 Fragments arranged by size: 2702 1463 1128 711 BsaBI GATnn'nnATC Cuts at: 5998 5998 Size: 6004 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2528 2586 3298 3811 Size: 53 83 186 936 995 58 712 513 Cuts at: 3811 4193 4828 275 Size: 382 635 1451 Fragments arranged by size: 1451 995 936 712 635 513 382 186 83 58 53 BsaWI w'CCGG_w Cuts at: 2697 4437 4835 5109 5256 5667 2697 Size: 1740 398 274 147 411 3034 Fragments arranged by size: 3034 1740 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2518 2548 2916 2946 3253 Size: 30 165 30 1115 30 368 30 307 Cuts at: 3253 3283 1178 Size: 30 3899 Fragments arranged by size: 3899 1115 368 307 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3067 1186 Size: 1881 4123 BseYI C'CCAG_C Cuts at: 2832 5207 5703 2832 Size: 2375 496 3133 Fragments arranged by size: 3133 2375 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2498 2735 1163 Size: 573 762 237 4432 Fragments arranged by size: 4432 762 573 237 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3454 4215 5243 1540 Size: 275 186 1453 761 1028 2301 Fragments arranged by size: 2301 1453 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2033 2340 2439 2943 3174 4065 4150 5221 2033 Size: 307 99 504 231 891 85 1071 2816 Fragments arranged by size: 2816 1071 891 504 307 231 99 85 BsmI GAATG_Cn' Cuts at: 2067 3515 3608 2067 Size: 1448 93 4463 Fragments arranged by size: 4463 1448 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3305 3305 Size: 6004 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2033 2340 2439 2943 3091 3099 3174 Size: 399 243 307 99 504 148 8 75 Cuts at: 3174 3323 4065 4150 5221 1391 Size: 149 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 504 399 307 243 149 148 99 85 75 8 BspEI T'CCGG_A Cuts at: 2697 5667 2697 Size: 2970 3034 BspHI T'CATG_A Cuts at: 3893 5623 3893 Size: 1730 4274 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2065 3359 844 Size: 1221 1294 3489 Fragments arranged by size: 3489 1294 1221 BsrBI CCG'CTC Cuts at: 1394 1823 3891 1394 Size: 429 2068 3507 Fragments arranged by size: 3507 2068 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4499 4673 65 Size: 1183 3251 174 1396 Fragments arranged by size: 3251 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4648 4835 1700 Size: 110 2838 187 2869 Fragments arranged by size: 2869 2838 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6004 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6004 BssSI C'ACGA_G Cuts at: 2032 2144 2516 4064 5076 2032 Size: 112 372 1548 1012 2960 Fragments arranged by size: 2960 1548 1012 372 112 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6004 BstBI TT'CG_AA Cuts at: 4808 4808 Size: 6004 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6004 BstYI r'GATC_y Cuts at: 1972 2017 2702 2730 2919 3141 3334 3701 4086 Size: 45 685 28 189 222 193 367 385 Cuts at: 4086 4103 5555 5641 5653 5999 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 685 385 367 346 222 193 189 86 45 28 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2555 3185 3230 3243 513 Size: 552 939 551 630 45 13 3274 Fragments arranged by size: 3274 939 630 552 551 45 13 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5265 Fragments arranged by size: 5265 683 56 BtsI GCAGTG_nn' Cuts at: 3520 4314 4334 3520 Size: 794 20 5190 Fragments arranged by size: 5190 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6004 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5969 DraI TTT'AAA Cuts at: 3308 3308 Size: 6004 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2465 5011 809 Size: 507 1149 2546 1802 Fragments arranged by size: 2546 1802 1149 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2011 2055 3059 Size: 54 1425 50 275 18 181 44 1004 Cuts at: 3059 3451 4340 5969 8 Size: 392 889 1629 43 Fragments arranged by size: 1629 1425 1004 889 392 275 181 54 50 44 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3451 1537 Size: 275 1639 4090 Fragments arranged by size: 4090 1639 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3934 1353 Size: 2581 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2695 4591 4965 5111 387 Size: 1064 138 1106 1896 374 146 1280 Fragments arranged by size: 1896 1280 1106 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2437 2437 Size: 6004 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2189 2258 2489 2828 3130 3176 4067 Size: 393 557 69 231 339 302 46 891 Cuts at: 4067 4664 5451 5719 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 891 787 597 557 393 339 302 268 231 69 46 EcoNI CCTnn'n_nnAGG Cuts at: 2516 2516 Size: 6004 EcoO109I rG'GnC_Cy Cuts at: 2323 2359 2689 2323 Size: 36 330 5638 Fragments arranged by size: 5638 330 36 EcoRI G'AATT_C Cuts at: 3313 3691 3313 Size: 378 5626 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2332 2332 Size: 6004 FspI TGC'GCA Cuts at: 2681 4510 2681 Size: 1829 4175 HaeII r_GCGC'y Cuts at: 1678 5151 1678 Size: 3473 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2020 2052 2094 2126 2518 Size: 32 135 32 448 32 42 32 392 Cuts at: 2518 2550 4645 4677 4719 4751 1373 Size: 32 2095 32 42 32 2626 Fragments arranged by size: 2626 2095 448 392 135 42 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3348 3594 669 Size: 988 281 1410 246 3079 Fragments arranged by size: 3079 1410 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6004 HpaI GTT'AAC Cuts at: 3594 3594 Size: 6004 KpnI G_GTAC'C Cuts at: 3329 3329 Size: 6004 MfeI C'AATT_G Cuts at: 3603 3603 Size: 6004 MluI A'CGCG_T Cuts at: 5880 5880 Size: 6004 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5118 5302 1305 Size: 3813 184 2007 Fragments arranged by size: 3813 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2013 2057 3061 10 Size: 54 1425 343 181 44 1004 2953 Fragments arranged by size: 2953 1425 1004 343 181 54 44 MslI CAynn'nnrTG Cuts at: 518 1166 2882 3962 4321 4480 518 Size: 648 1716 1080 359 159 2042 Fragments arranged by size: 2042 1716 1080 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2121 2161 2464 2557 2566 3420 4097 5245 Size: 515 40 303 93 9 854 677 1148 Cuts at: 5245 5490 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 854 677 515 303 245 93 40 9 NcoI C'CATG_G Cuts at: 513 1065 2004 3185 513 Size: 552 939 1181 3332 Fragments arranged by size: 3332 1181 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6004 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6004 NotI GC'GGCC_GC Cuts at: 3451 3451 Size: 6004 NspI r_CATG'y Cuts at: 1132 1229 2984 3109 3362 4907 1132 Size: 97 1755 125 253 1545 2229 Fragments arranged by size: 2229 1755 1545 253 125 97 PasI CC'CwG_GG Cuts at: 1348 2263 3067 1348 Size: 915 804 4285 Fragments arranged by size: 4285 915 804 PciI A'CATG_T Cuts at: 3105 4903 3105 Size: 1798 4206 PflMI CCAn_nnn'nTGG Cuts at: 1108 2149 1108 Size: 1041 4963 PmeI GTTT'AAAC Cuts at: 3308 3308 Size: 6004 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2678 2710 4073 4105 5586 5618 2678 Size: 32 1363 32 1481 32 3064 Fragments arranged by size: 3064 1481 1363 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2359 2359 Size: 6004 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6004 PsiI TTA'TAA Cuts at: 3574 3574 Size: 6004 PstI C_TGCA'G Cuts at: 830 2076 3356 830 Size: 1246 1280 3478 Fragments arranged by size: 3478 1280 1246 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6004 PvuII CAG'CTG Cuts at: 2121 2464 2121 Size: 343 5661 SacI G_AGCT'C Cuts at: 2439 2439 Size: 6004 SacII CC_GC'GG Cuts at: 2558 2558 Size: 6004 SalI G'TCGA_C Cuts at: 1936 3346 1936 Size: 1410 4594 SbfI CC_TGCA'GG Cuts at: 3356 3356 Size: 6004 ScaI AGT'ACT Cuts at: 3131 4252 5964 3131 Size: 1121 1712 3171 Fragments arranged by size: 3171 1712 1121 SfcI C'TryA_G Cuts at: 826 1045 2072 3352 4487 5168 5359 826 Size: 219 1027 1280 1135 681 191 1471 Fragments arranged by size: 1471 1280 1135 1027 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6004 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6004 SmaI CCC'GGG Cuts at: 1872 2993 3331 1872 Size: 1121 338 4545 Fragments arranged by size: 4545 1121 338 SmlI C'TyrA_G Cuts at: 820 1017 1943 4108 5009 5271 820 Size: 197 926 2165 901 262 1553 Fragments arranged by size: 2165 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3795 493 Size: 3302 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6004 SphI G_CATG'C Cuts at: 1132 2984 3362 1132 Size: 1852 378 3774 Fragments arranged by size: 3774 1852 378 SspI AAT'ATT Cuts at: 5 52 3928 5 Size: 47 3876 2081 Fragments arranged by size: 3876 2081 47 StuI AGG'CCT Cuts at: 1517 2676 2855 1517 Size: 1159 179 4666 Fragments arranged by size: 4666 1159 179 StyI C'CwwG_G Cuts at: 513 1065 2004 2749 3185 3443 513 Size: 552 939 745 436 258 3074 Fragments arranged by size: 3074 939 745 552 436 258 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4031 4048 4201 4386 4831 325 Size: 1255 2451 17 153 185 445 1498 Fragments arranged by size: 2451 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2181 2304 3129 Size: 274 80 33 51 1443 204 123 825 Cuts at: 3129 4250 5962 96 Size: 1121 1712 138 Fragments arranged by size: 1712 1443 1121 825 274 204 138 123 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2866 2898 5964 5996 2866 Size: 32 3066 32 2874 Fragments arranged by size: 3066 2874 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6004 XbaI T'CTAG_A Cuts at: 3340 3340 Size: 6004 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6004 XmaI C'CCGG_G Cuts at: 1870 2991 3329 1870 Size: 1121 338 4545 Fragments arranged by size: 4545 1121 338 XmnI GAAnn'nnTTC Cuts at: 2813 3107 4133 2813 Size: 294 1026 4684 Fragments arranged by size: 4684 1026 294 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3299 3812 276 Size: 53 83 186 936 1765 513 2468 Fragments arranged by size: 2468 1765 936 513 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI