Restriction map for
pFN21AE1927
(Linear) (Six-base) MAPSORT of: KIEE1927.seq Check: 4255 from: 1 to: 1299
KIEE1927.orf
With 182 enzymes: SgfI *
July 15, 2010 18:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 60 1299
Size: 60 1239
AatII G_ACGT'C
Cuts at: 0 1296 1299
Size: 1296 3
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 823 1125 1299
Size: 823 302 174
Fragments arranged by size:
823 302 174
AflIII A'CryG_T
Cuts at: 0 99 1100 1299
Size: 99 1001 199
Fragments arranged by size:
1001 199 99
AhdI GACnn_n'nnGTC
Cuts at: 0 103 1299
Size: 103 1196
AleI CACnn'nnGTG
Cuts at: 0 877 1299
Size: 877 422
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 238 270 1299
Size: 238 32 1029
Fragments arranged by size:
1029 238 32
AlwNI CAG_nnn'CTG
Cuts at: 0 119 159 507 1299
Size: 119 40 348 792
Fragments arranged by size:
792 348 119 40
ApaLI G'TGCA_C
Cuts at: 0 331 1299
Size: 331 968
ApoI r'AATT_y
Cuts at: 0 1044 1299
Size: 1044 255
AvaI C'yCGr_G
Cuts at: 0 865 986 1299
Size: 865 121 313
Fragments arranged by size:
865 313 121
BamHI G'GATC_C
Cuts at: 0 12 1299
Size: 12 1287
BanI G'GyrC_C
Cuts at: 0 1155 1176 1299
Size: 1155 21 123
Fragments arranged by size:
1155 123 21
BanII G_rGCy'C
Cuts at: 0 434 1299
Size: 434 865
BbsI GAAGACnn'nnnn_
Cuts at: 0 102 811 1026 1296 1299
Size: 102 709 215 270 3
Fragments arranged by size:
709 270 215 102 3
BbvCI CC'TCA_GC
Cuts at: 0 637 1299
Size: 637 662
BglII A'GATC_T
Cuts at: 0 914 1136 1299
Size: 914 222 163
Fragments arranged by size:
914 222 163
BlpI GC'TnA_GC
Cuts at: 0 22 1299
Size: 22 1277
Bme1580I G_kGCm'C
Cuts at: 0 335 1086 1094 1299
Size: 335 751 8 205
Fragments arranged by size:
751 335 205 8
BmgBI CAC'GTC
Cuts at: 0 48 100 1299
Size: 48 52 1199
Fragments arranged by size:
1199 52 48
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 253 484 1171 1299
Size: 184 69 231 687 128
Fragments arranged by size:
687 231 184 128 69
Bpu10I CC'TnA_GC
Cuts at: 0 637 1299
Size: 637 662
BsaI GGTCTCn'nnnn_
Cuts at: 0 1074 1299
Size: 1074 225
BsaAI yAC'GTr
Cuts at: 0 327 1299
Size: 327 972
BsaHI Gr'CG_yC
Cuts at: 0 523 581 1293 1299
Size: 523 58 712 6
Fragments arranged by size:
712 523 58 6
BsaWI w'CCGG_w
Cuts at: 0 692 1299
Size: 692 607
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 513 543 911 941 1248 1278 1299
Size: 513 30 368 30 307 30 21
Fragments arranged by size:
513 368 307 30 30 30 21
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1062 1299
Size: 1062 237
BseYI C'CCAG_C
Cuts at: 0 827 1299
Size: 827 472
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 493 730 1299
Size: 493 237 569
Fragments arranged by size:
569 493 237
BsiHKAI G_wGCw'C
Cuts at: 0 28 335 434 938 1169 1299
Size: 28 307 99 504 231 130
Fragments arranged by size:
504 307 231 130 99 28
BsmI GAATG_Cn'
Cuts at: 0 62 1299
Size: 62 1237
Bsp1286I G_dGCh'C
Cuts at: 0 28 335 434 938 1086 1094 1169 1299
Size: 28 307 99 504 148 8 75 130
Fragments arranged by size:
504 307 148 130 99 75 28 8
BspEI T'CCGG_A
Cuts at: 0 692 1299
Size: 692 607
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 60 1299
Size: 60 1239
BssSI C'ACGA_G
Cuts at: 0 27 139 511 1299
Size: 27 112 372 788
Fragments arranged by size:
788 372 112 27
BstYI r'GATC_y
Cuts at: 0 12 697 725 914 1136 1299
Size: 12 685 28 189 222 163
Fragments arranged by size:
685 222 189 163 28 12
BtgI C'CryG_G
Cuts at: 0 550 1180 1225 1238 1299
Size: 550 630 45 13 61
Fragments arranged by size:
630 550 61 45 13
DrdI GACnn_nn'nnGTC
Cuts at: 0 460 1299
Size: 460 839
EaeI y'GGCC_r
Cuts at: 0 6 50 1054 1299
Size: 6 44 1004 245
Fragments arranged by size:
1004 245 44 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 690 1299
Size: 690 609
EcoICRI GAG'CTC
Cuts at: 0 432 1299
Size: 432 867
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 253 484 823 1125 1171 1299
Size: 184 69 231 339 302 46 128
Fragments arranged by size:
339 302 231 184 128 69 46
EcoNI CCTnn'n_nnAGG
Cuts at: 0 511 1299
Size: 511 788
EcoO109I rG'GnC_Cy
Cuts at: 0 318 354 684 1299
Size: 318 36 330 615
Fragments arranged by size:
615 330 318 36
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 327 1299
Size: 327 972
FspI TGC'GCA
Cuts at: 0 676 1299
Size: 676 623
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 15 47 89 121 513 545 1299
Size: 15 32 42 32 392 32 754
Fragments arranged by size:
754 392 42 32 32 32 15
MscI TGG'CCA
Cuts at: 0 8 52 1056 1299
Size: 8 44 1004 243
Fragments arranged by size:
1004 243 44 8
MslI CAynn'nnrTG
Cuts at: 0 877 1299
Size: 877 422
MspA1I CmG'CkG
Cuts at: 0 116 156 459 552 561 1299
Size: 116 40 303 93 9 738
Fragments arranged by size:
738 303 116 93 40 9
NcoI C'CATG_G
Cuts at: 0 1180 1299
Size: 1180 119
NspI r_CATG'y
Cuts at: 0 979 1104 1299
Size: 979 125 195
Fragments arranged by size:
979 195 125
PasI CC'CwG_GG
Cuts at: 0 258 1062 1299
Size: 258 804 237
Fragments arranged by size:
804 258 237
PciI A'CATG_T
Cuts at: 0 1100 1299
Size: 1100 199
PflMI CCAn_nnn'nTGG
Cuts at: 0 144 1299
Size: 144 1155
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 673 705 1299
Size: 673 32 594
Fragments arranged by size:
673 594 32
PpuMI rG'GwC_Cy
Cuts at: 0 354 1299
Size: 354 945
PstI C_TGCA'G
Cuts at: 0 71 1299
Size: 71 1228
PvuII CAG'CTG
Cuts at: 0 116 459 1299
Size: 116 343 840
Fragments arranged by size:
840 343 116
SacI G_AGCT'C
Cuts at: 0 434 1299
Size: 434 865
SacII CC_GC'GG
Cuts at: 0 553 1299
Size: 553 746
ScaI AGT'ACT
Cuts at: 0 1126 1299
Size: 1126 173
SfcI C'TryA_G
Cuts at: 0 67 1299
Size: 67 1232
SmaI CCC'GGG
Cuts at: 0 988 1299
Size: 988 311
SphI G_CATG'C
Cuts at: 0 979 1299
Size: 979 320
StuI AGG'CCT
Cuts at: 0 671 850 1299
Size: 671 179 449
Fragments arranged by size:
671 449 179
StyI C'CwwG_G
Cuts at: 0 744 1180 1299
Size: 744 436 119
Fragments arranged by size:
744 436 119
TatI w'GTAC_w
Cuts at: 0 176 299 1124 1299
Size: 176 123 825 175
Fragments arranged by size:
825 176 175 123
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 861 893 1299
Size: 861 32 406
Fragments arranged by size:
861 406 32
XmaI C'CCGG_G
Cuts at: 0 986 1299
Size: 986 313
XmnI GAAnn'nnTTC
Cuts at: 0 808 1102 1299
Size: 808 294 197
Fragments arranged by size:
808 294 197
ZraI GAC'GTC
Cuts at: 0 1294 1299
Size: 1294 5
Enzymes that do cut:
AarI AatII AcuI AflIII AhdI AleI AloI AlwNI
ApaLI ApoI AvaI BamHI BanI BanII BbsI BbvCI
BglII BlpI Bme1580I BmgBI BpmI Bpu10I BsaI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
Bsp1286I BspEI BspMI BssSI BstYI BtgI DrdI EaeI
EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI Hin4I
MscI MslI MspA1I NcoI NspI PasI PciI PflMI
PpiI PpuMI PstI PvuII SacI SacII ScaI SfcI
SmaI SphI StuI StyI TatI TstI XmaI XmnI
ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AgeI AlfI ApaI
AscI AseI AsiSI AvrII BaeI BbeI BcgI BciVI
BclI BfrBI BglI BmrI BmtI BplI BpuEI BsaBI
BsiEI BsiWI BsmBI BspHI BsrBI BsrDI BsrFI BsrGI
BssHII BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI
BtsI ClaI CspCI DraI DraIII EagI EarI EcoRI
EcoRV FseI FspAI HaeII HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI PfoI PmeI PmlI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SalI
SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI
SmlI SnaBI SpeI SrfI SspI SwaI TaqII Tth111I
XbaI XcmI XhoI