Restriction map for
pFN21AE1927
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# NO CUT ENZYMES ( 113 enzymes) #
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Acc65I AccI AclI AflII AgeI
AocI Aor51HI ApaI AscI AseI
AsnI Asp718I AspI AvrII BbeI
BbrPI BcgI BciVI BclI BfaI
BfrI BglI BlnI BsaBI BsiEI
BsiWI Bsp1407I BspDI BspHI BsrBI
BsrDI BsrFI BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraI DraIII EagI EarI EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI HaeII
HincII HindII HindIII HpaI KasI
KpnI Ksp632I MaeI MamI MfeI
MluI MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
Sse8387I SspBI SspI SwaI Tth111I
XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AatII 1292 GACGTC GACGT^C
AccIII 692 TCCGGA T^CCGGA
AcsI 1044 GAATTT [GA]^AATT[CT]
AhdI 98 GACGTGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 331 GTGCAC G^TGCAC
ApaLI 331 GTGCAC G^TGCAC
ApoI 1044 GAATTT [GA]^AATT[CT]
AspEI 98 GACGTGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 674 TGCGCA TGC^GCA
BamHI 12 GGATCC G^GATCC
BanII 430 GAGCTC G[GA]GC[CT]^C
BbvCI 636 GCTGAGG CCTCAGC(-5/-2)
BlpI 21 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 21 GCTGAGC GC^T[ACGT]AGC
BsaAI 325 TACGTG [CT]AC^GT[GA]
BsaI 1068 GGTCTC GGTCTC(1/5)
BsaWI 692 TCCGGA [AT]^CCGG[AT]
BseAI 692 TCCGGA T^CCGGA
BseRI 1071 CTCCTC GAGGAG(10/8)
BsmBI 1294 CGTCTC CGTCTC(1/5)
BsmI 62 GCATTC GAATGC(1/-1)
BspEI 692 TCCGGA T^CCGGA
BspLU11I 1100 ACATGT A^CATGT
BspMI 69 GCAGGT ACCTGC(4/8)
CelII 21 GCTGAGC GC^T[ACGT]AGC
DrdI 454 GACCAGCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 98 GACGTGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 507 CCTGCACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 558 CCCGC CCCGC(4/6)
FspI 674 TGCGCA TGC^GCA
KspI 550 CCGCGG CCGC^GG
MroI 692 TCCGGA T^CCGGA
MseI 446 TTAA T^TAA
MslI 873 CACCTGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 1180 CCATGG C^CATGG
PflMI 138 CCACGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 353 AGGTCCT [GA]G^G[AT]CC[CT]
PstI 67 CTGCAG CTGCA^G
SacI 430 GAGCTC GAGCT^C
SacII 550 CCGCGG CCGC^GG
ScaI 1124 AGTACT AGT^ACT
SfcI 67 CTGCAG C^T[GA][CT]AG
SmaI 986 CCCGGG CCC^GGG
SphI 975 GCATGC GCATG^C
TfiI 861 GATTC G^A[AT]TC
Tru9I 446 TTAA T^TAA
Tsp509I 1045 AATT ^AATT
Van91I 138 CCACGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 986 CCCGGG C^CCGGG
XmaI 986 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 25 enzymes) #
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AccII 551 CGCG CG^CG
721 CGCG
AflIII 99 ACGTGT A^C[GA][CT]GT
1100 ACATGT
Asp700I 804 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1098 GAACATGTTC
AvaI 865 CTCGGG C^[CT]CG[GA]G
986 CCCGGG
BanI 1155 GGCACC G^G[CT][GA]CC
1176 GGCACC
BcnI 986 CCCGG CC^[GC]GG
987 CCGGG
BglII 914 AGATCT A^GATCT
1136 AGATCT
BsgI 508 CTGCAC GTGCAG(16/14)
709 GTGCAG
BsmAI 1069 GTCTC GTCTC(1/5)
1295 GTCTC
BsoBI 865 CTCGGG C^[CT]CG[GA]G
986 CCCGGG
BsrI 183 ACTGG ACTGG(1/-1)
901 ACTGG
BstUI 551 CGCG CG^CG
721 CGCG
EcoT14I 744 CCAAGG C^C[AT][AT]GG
1180 CCATGG
HgaI 522 GACGC GACGC(5/10)
581 GCGTC
MvnI 551 CGCG CG^CG
721 CGCG
NciI 986 CCCGG CC^[GC]GG
987 CCGGG
NspI 975 GCATGC [GA]CATG^[CT]
1100 ACATGT
PleI 30 GAGTC GAGTC(4/5)
1269 GAGTC
PvuII 114 CAGCTG CAG^CTG
457 CAGCTG
SfaNI 126 GATGC GCATC(5/9)
995 GCATC
StuI 669 AGGCCT AGG^CCT
848 AGGCCT
StyI 744 CCAAGG C^C[AT][AT]GG
1180 CCATGG
TaqI 290 TCGA T^CGA
1121 TCGA
TthHB8I 290 TCGA T^CGA
1121 TCGA
XmnI 804 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1098 GAACATGTTC
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# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI AspHI AvaII BalI BbsI
BbvI BmyI BpmI BpuAI BsaHI
BsaJI BsiHKAI BsiYI BslI BsmFI
Bsp1286I BssKI BssSI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EcoO109I
EcoRII Fnu4HI FokI HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI TseI Tsp45I TspRI XhoII