SPRED2 ORF clone pFN21AE1890

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE1890

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Clone info.

Species

human

Clone Name

pFN21AE1890 Sequence (with vector) Restriction Map

Product ID

FHC28706

ORF Sequence

DNA sequence (1254 bp)
Protein sequence (418 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB590946]

Gene

KIEE1890

Symbol

SPRED2

Alias : Spred-2

Description

sprouty-related, EVH1 domain containing 2, transcript variant 1

Original Clone ID

cs02790

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1254 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 418 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS33211.1 6.6e-161 100.0 SPRED2 CCDS46308.1 8.5e-158 99.5 SPRED2 CCDS42560.1 4.9e-49 39.4 SPRED3 CCDS32193.1 4.6e-46 53.1 SPRED1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR000697 14 118 PF00568 EVH1 IPR007875 306 411 PF05210 Sprouty HMMSmart IPR000697 8 119 SM00461 EVH1 ProfileScan IPR000697 5 122 PS50229 EVH1 IPR023337 201 257 PS51488 c-Kit-binding domain IPR007875 308 416 PS51227 Sprouty
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 374 FCLRWMALIALSFLAPCMCCYLP 396 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_861449 418 609292 100.0 100.0 NP_001121682 415 609292 99.5 98.6 XP_006723282 410 609293 39.4 97.1 NP_001035987 410 609293 39.4 97.1 XP_011519591 423 609291,611431 53.1 95.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE1890 Download

>KIEE1890  1254 bp
ATGACCGAAGAAACACACCCAGACGATGACAGCTATATTGTGCGTGTCAAGGCTGTGGTT
ATGACCAGAGATGACTCCAGCGGGGGATGGTTCCCACAGGAAGGAGGCGGGATCAGTCGC
GTCGGGGTCTGTAAGGTCATGCACCCCGAAGGCAATGGACGAAGCGGCTTTCTCATCCAT
GGTGAACGACAGAAAGACAAACTGGTGGTATTGGAATGCTATGTAAGAAAGGACTTGGTC
TACACCAAAGCCAATCCAACGTTTCATCACTGGAAGGTCGATAATAGGAAGTTTGGACTT
ACTTTCCAAAGCCCTGCTGATGCCCGAGCCTTTGACAGGGGAGTAAGGAAAGCAATCGAA
GACCTTATAGAAGGTTCAACAACGTCATCTTCCACCATCCATAATGAAGCTGAGCTTGGC
GATGATGACGTTTTTACAACAGCTACAGACAGTTCTTCTAATTCCTCTCAGAAGAGAGAG
CAACCTACTCGGACAATCTCCTCTCCCACATCCTGTGAGCACCGGAGGATTTATACCCTG
GGCCACCTCCACGACTCATACCCCACAGACCACTATCACCTCGATCAGCCGATGCCAAGG
CCCTACCGCCAGGTGAGCTTCCCGGACGACGACGAGGAGATCGTGCGCATCAACCCCCGG
GAGAAGATCTGGATGACGGGGTACGAGGATTACCGGCACGCACCCGTCAGGGGCAAGTAC
CCGGACCCCTCGGAGGACGCGGACTCCTCCTACGTGCGCTTCGCCAAGGGCGAGGTCCCC
AAGCATGACTACAACTACCCCTACGTGGACTCCTCAGACTTTGGCCTAGGCGAGGACCCC
AAAGGCCGCGGGGGCAGCGTGATCAAGACGCAGCCCTCCCGGGGCAAGTCGCGGCGGCGG
AAGGAGGACGGAGAGCGCTCGCGGTGCGTGTACTGCAGGGACATGTTCAACCACGAGGAG
AACCGCCGGGGCCACTGCCAGGACGCGCCCGACTCCGTGAGAACTTGCATCCGCCGGGTG
AGCTGCATGTGGTGCGCGGACAGCATGCTCTATCACTGTATGTCGGACCCCGAGGGAGAC
TATACAGACCCTTGCTCGTGCGATACTAGCGACGAGAAGTTTTGCCTCCGGTGGATGGCT
CTTATTGCCTTGTCTTTCCTGGCCCCCTGTATGTGCTGTTACCTGCCCCTTCGGGCCTGC
TACCACTGCGGAGTGATGTGCAGGTGCTGTGGCGGGAAGCACAAAGCGGCCGCG

ORF Protein

Cloned ORF protein sequence for pFN21AE1890 Download

>KIEE1890  418 aa
MTEETHPDDDSYIVRVKAVVMTRDDSSGGWFPQEGGGISRVGVCKVMHPEGNGRSGFLIH
GERQKDKLVVLECYVRKDLVYTKANPTFHHWKVDNRKFGLTFQSPADARAFDRGVRKAIE
DLIEGSTTSSSTIHNEAELGDDDVFTTATDSSSNSSQKREQPTRTISSPTSCEHRRIYTL
GHLHDSYPTDHYHLDQPMPRPYRQVSFPDDDEEIVRINPREKIWMTGYEDYRHAPVRGKY
PDPSEDADSSYVRFAKGEVPKHDYNYPYVDSSDFGLGEDPKGRGGSVIKTQPSRGKSRRR
KEDGERSRCVYCRDMFNHEENRRGHCQDAPDSVRTCIRRVSCMWCADSMLYHCMSDPEGD
YTDPCSCDTSDEKFCLRWMALIALSFLAPCMCCYLPLRACYHCGVMCRCCGGKHKAAA

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE1890 Download

>pFN21AE1890  5959 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGACCGAAGAAACACACCCAGACGATGACAGCTA
TATTGTGCGTGTCAAGGCTGTGGTTATGACCAGAGATGACTCCAGCGGGGGATGGTTCCC
ACAGGAAGGAGGCGGGATCAGTCGCGTCGGGGTCTGTAAGGTCATGCACCCCGAAGGCAA
TGGACGAAGCGGCTTTCTCATCCATGGTGAACGACAGAAAGACAAACTGGTGGTATTGGA
ATGCTATGTAAGAAAGGACTTGGTCTACACCAAAGCCAATCCAACGTTTCATCACTGGAA
GGTCGATAATAGGAAGTTTGGACTTACTTTCCAAAGCCCTGCTGATGCCCGAGCCTTTGA
CAGGGGAGTAAGGAAAGCAATCGAAGACCTTATAGAAGGTTCAACAACGTCATCTTCCAC
CATCCATAATGAAGCTGAGCTTGGCGATGATGACGTTTTTACAACAGCTACAGACAGTTC
TTCTAATTCCTCTCAGAAGAGAGAGCAACCTACTCGGACAATCTCCTCTCCCACATCCTG
TGAGCACCGGAGGATTTATACCCTGGGCCACCTCCACGACTCATACCCCACAGACCACTA
TCACCTCGATCAGCCGATGCCAAGGCCCTACCGCCAGGTGAGCTTCCCGGACGACGACGA
GGAGATCGTGCGCATCAACCCCCGGGAGAAGATCTGGATGACGGGGTACGAGGATTACCG
GCACGCACCCGTCAGGGGCAAGTACCCGGACCCCTCGGAGGACGCGGACTCCTCCTACGT
GCGCTTCGCCAAGGGCGAGGTCCCCAAGCATGACTACAACTACCCCTACGTGGACTCCTC
AGACTTTGGCCTAGGCGAGGACCCCAAAGGCCGCGGGGGCAGCGTGATCAAGACGCAGCC
CTCCCGGGGCAAGTCGCGGCGGCGGAAGGAGGACGGAGAGCGCTCGCGGTGCGTGTACTG
CAGGGACATGTTCAACCACGAGGAGAACCGCCGGGGCCACTGCCAGGACGCGCCCGACTC
CGTGAGAACTTGCATCCGCCGGGTGAGCTGCATGTGGTGCGCGGACAGCATGCTCTATCA
CTGTATGTCGGACCCCGAGGGAGACTATACAGACCCTTGCTCGTGCGATACTAGCGACGA
GAAGTTTTGCCTCCGGTGGATGGCTCTTATTGCCTTGTCTTTCCTGGCCCCCTGTATGTG
CTGTTACCTGCCCCTTCGGGCCTGCTACCACTGCGGAGTGATGTGCAGGTGCTGTGGCGG
GAAGCACAAAGCGGCCGCGGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGA
GTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGT
TGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCA
GACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAA
TGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAAT
AAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGG
GAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATC
CTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGT
CAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACT
TTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATG
TATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCT
GTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCA
CGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCC
GAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCC
CGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTG
GTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTA
TGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATC
GGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTT
GATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATG
CCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCT
TCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGC
TCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCT
CGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTAC
ACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCC
TCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAG
CTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACA
TGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTT
TCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGC
GAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCT
CTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCG
TGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCA
AGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACT
ATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTA
ACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTA
ACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCT
TCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTT
TTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGA
TCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCA
TGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGC
ACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATT
AAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTT
ATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGC
CGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCAC
TTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCG
GCGTAGAGGATCGAGATCT