(Linear) (Six-base) MAPSORT of: KIEE1890.seq Check: 4418 from: 1 to: 1254 KIEE1890.orf With 182 enzymes: SgfI * July 15, 2010 18:50 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1211 1254 Size: 1211 43 AccI GT'mk_AC Cuts at: 0 239 1254 Size: 239 1015 AclI AA'CG_TT Cuts at: 0 259 1254 Size: 259 995 AfeI AGC'GCT Cuts at: 0 916 1254 Size: 916 338 AflIII A'CryG_T Cuts at: 0 941 1254 Size: 941 313 AlwNI CAG_nnn'CTG Cuts at: 0 1226 1254 Size: 1226 28 AvaI C'yCGr_G Cuts at: 0 323 656 878 1069 1254 Size: 323 333 222 191 185 Fragments arranged by size: 333 323 222 191 185 AvrII C'CTAG_G Cuts at: 0 825 1254 Size: 825 429 BbsI GAAGACnn'nnnn_ Cuts at: 0 365 1254 Size: 365 889 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 469 503 1254 Size: 469 34 751 Fragments arranged by size: 751 469 34 BclI T'GATC_A Cuts at: 0 860 1254 Size: 860 394 BglI GCCn_nnn'nGGC Cuts at: 0 851 1254 Size: 851 403 BglII A'GATC_T Cuts at: 0 665 1254 Size: 665 589 BlpI GC'TnA_GC Cuts at: 0 410 1254 Size: 410 844 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 60 1254 Size: 60 1194 BsaAI yAC'GTr Cuts at: 0 753 804 1254 Size: 753 51 450 Fragments arranged by size: 753 450 51 BsaWI w'CCGG_w Cuts at: 0 521 1127 1254 Size: 521 606 127 Fragments arranged by size: 606 521 127 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 727 757 793 823 1254 Size: 727 30 36 30 431 Fragments arranged by size: 727 431 36 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 489 649 735 801 970 1254 Size: 489 160 86 66 169 284 Fragments arranged by size: 489 284 169 160 86 66 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1239 1254 Size: 1239 15 BsiEI CG_ry'CG Cuts at: 0 1250 1254 Size: 1250 4 BsiHKAI G_wGCw'C Cuts at: 0 521 1254 Size: 521 733 BsmI GAATG_Cn' Cuts at: 0 220 1254 Size: 220 1034 Bsp1286I G_dGCh'C Cuts at: 0 521 1254 Size: 521 733 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1190 1211 1254 Size: 1190 21 43 Fragments arranged by size: 1190 43 21 BsrDI GCAATG_nn' Cuts at: 0 159 1254 Size: 159 1095 BsrFI r'CCGG_y Cuts at: 0 692 1254 Size: 692 562 BssSI C'ACGA_G Cuts at: 0 952 1095 1254 Size: 952 143 159 Fragments arranged by size: 952 159 143 BstAPI GCAn_nnn'nTGC Cuts at: 0 1031 1254 Size: 1031 223 BstYI r'GATC_y Cuts at: 0 665 1254 Size: 665 589 BtgI C'CryG_G Cuts at: 0 177 846 1254 Size: 177 669 408 Fragments arranged by size: 669 408 177 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 434 1254 Size: 434 820 BtsI GCAGTG_nn' Cuts at: 0 972 1203 1254 Size: 972 231 51 Fragments arranged by size: 972 231 51 EaeI y'GGCC_r Cuts at: 0 1247 1254 Size: 1247 7 EagI C'GGCC_G Cuts at: 0 1247 1254 Size: 1247 7 EarI CTCTTCn'nnn_ Cuts at: 0 466 1254 Size: 466 788 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 912 1000 1254 Size: 912 88 254 Fragments arranged by size: 912 254 88 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 60 1254 Size: 60 1194 EcoNI CCTnn'n_nnAGG Cuts at: 0 333 829 1254 Size: 333 496 425 Fragments arranged by size: 496 425 333 EcoO109I rG'GnC_Cy Cuts at: 0 599 774 834 1254 Size: 599 175 60 420 Fragments arranged by size: 599 420 175 60 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 430 1254 Size: 430 824 FspI TGC'GCA Cuts at: 0 646 1254 Size: 646 608 FspAI rTGC'GCAy Cuts at: 0 646 1254 Size: 646 608 HaeII r_GCGC'y Cuts at: 0 918 1254 Size: 918 336 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 95 127 727 759 1078 1110 1254 Size: 95 32 600 32 319 32 144 Fragments arranged by size: 600 319 144 95 32 32 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 280 1043 1254 Size: 280 763 211 Fragments arranged by size: 763 280 211 MspA1I CmG'CkG Cuts at: 0 80 848 1254 Size: 80 768 406 Fragments arranged by size: 768 406 80 NcoI C'CATG_G Cuts at: 0 177 1254 Size: 177 1077 NotI GC'GGCC_GC Cuts at: 0 1247 1254 Size: 1247 7 NspI r_CATG'y Cuts at: 0 945 1029 1047 1254 Size: 945 84 18 207 Fragments arranged by size: 945 207 84 18 PasI CC'CwG_GG Cuts at: 0 537 1254 Size: 537 717 PciI A'CATG_T Cuts at: 0 941 1254 Size: 941 313 PfoI T'CCnGG_A Cuts at: 0 620 1254 Size: 620 634 PpuMI rG'GwC_Cy Cuts at: 0 774 834 1254 Size: 774 60 420 Fragments arranged by size: 774 420 60 PstI C_TGCA'G Cuts at: 0 937 1254 Size: 937 317 SacII CC_GC'GG Cuts at: 0 849 1254 Size: 849 405 SfcI C'TryA_G Cuts at: 0 443 933 1254 Size: 443 490 321 Fragments arranged by size: 490 443 321 SmaI CCC'GGG Cuts at: 0 658 880 1254 Size: 658 222 374 Fragments arranged by size: 658 374 222 SphI G_CATG'C Cuts at: 0 1047 1254 Size: 1047 207 StyI C'CwwG_G Cuts at: 0 177 595 764 825 1254 Size: 177 418 169 61 429 Fragments arranged by size: 429 418 177 169 61 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 19 32 1254 Size: 19 13 1222 Fragments arranged by size: 1222 19 13 TatI w'GTAC_w Cuts at: 0 929 1254 Size: 929 325 Tth111I GACn'n_nGTC Cuts at: 0 235 1254 Size: 235 1019 XmaI C'CCGG_G Cuts at: 0 656 878 1254 Size: 656 222 376 Fragments arranged by size: 656 376 222 Enzymes that do cut: AarI AccI AclI AfeI AflIII AlwNI AvaI AvrII BbsI BcgI BclI BglI BglII BlpI BpmI BsaAI BsaWI BsaXI BseRI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrDI BsrFI BssSI BstAPI BstYI BtgI BtgZI BtsI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I FalI FspI FspAI HaeII Hin4I MmeI MspA1I NcoI NotI NspI PasI PciI PfoI PpuMI PstI SacII SfcI SmaI SphI StyI TaqII TatI Tth111I XmaI Enzymes that do not cut: AatII Acc65I AcuI AflII AgeI AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI BaeI BamHI BanI BanII BbeI BbvCI BciVI BfrBI Bme1580I BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaI BsaBI BsaHI BseYI BsiWI BsmBI BspEI BspHI BsrBI BsrGI BssHII BstBI BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EcoICRI EcoRI EcoRV FseI HincII HindIII HpaI KasI KpnI MfeI MluI MscI MslI NaeI NarI NdeI NgoMIV NheI NruI NsiI PacI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SrfI SspI StuI SwaI TstI XbaI XcmI XhoI XmnI ZraI