(Circular) (Six-base) MAPSORT of: pFN21AE1890.seq Check: 2513 from: 1 to: 5959 pFN21AE1890 5959 bp With 182 enzymes: SgfI * August 16, 2010 14:50 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3216 3216 Size: 5959 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3769 278 Size: 53 83 186 936 2233 2468 Fragments arranged by size: 2468 2233 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2244 3302 1937 Size: 307 1058 4594 Fragments arranged by size: 4594 1058 307 Acc65I G'GTAC_C Cuts at: 3280 3280 Size: 5959 AclI AA'CG_TT Cuts at: 1563 2264 4086 4459 1563 Size: 701 1822 373 3063 Fragments arranged by size: 3063 1822 701 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4022 5406 5674 1632 Size: 2390 1384 268 1917 Fragments arranged by size: 2390 1917 1384 268 AfeI AGC'GCT Cuts at: 2921 2921 Size: 5959 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5762 AflIII A'CryG_T Cuts at: 1203 2946 4858 5835 1203 Size: 1743 1912 977 1327 Fragments arranged by size: 1912 1743 1327 977 AgeI A'CCGG_T Cuts at: 4790 4790 Size: 5959 AhdI GACnn_n'nnGTC Cuts at: 4688 4688 Size: 5959 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5959 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5959 AlwNI CAG_nnn'CTG Cuts at: 3231 3702 5274 3231 Size: 471 1572 3916 Fragments arranged by size: 3916 1572 471 ApaLI G'TGCA_C Cuts at: 4016 5172 4016 Size: 1156 4803 ApoI r'AATT_y Cuts at: 1493 3268 3494 3646 5755 1493 Size: 1775 226 152 2109 1697 Fragments arranged by size: 2109 1775 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4513 160 Size: 872 3481 1606 Fragments arranged by size: 3481 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5959 AvaI C'yCGr_G Cuts at: 1870 1943 2328 2661 2883 3074 3284 1870 Size: 73 385 333 222 191 210 4545 Fragments arranged by size: 4545 385 333 222 210 191 73 AvrII C'CTAG_G Cuts at: 2830 2830 Size: 5959 BamHI G'GATC_C Cuts at: 3289 3656 3289 Size: 367 5592 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3280 4735 618 Size: 325 217 627 1493 1455 1842 Fragments arranged by size: 1842 1493 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3278 1391 Size: 1887 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2370 928 Size: 971 471 4517 Fragments arranged by size: 4517 971 471 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2474 2508 4150 4184 1903 Size: 34 537 34 1642 34 3678 Fragments arranged by size: 3678 1642 537 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3851 5061 3851 Size: 1210 4749 BclI T'GATC_A Cuts at: 1548 2865 1548 Size: 1317 4642 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2856 4570 136 Size: 107 122 71 631 605 1184 1714 1525 Fragments arranged by size: 1714 1525 1184 631 605 122 107 71 BglII A'GATC_T Cuts at: 2670 5954 2670 Size: 3284 2675 BlpI GC'TnA_GC Cuts at: 2415 3376 2415 Size: 961 4998 Bme1580I G_kGCm'C Cuts at: 1790 4020 5176 1790 Size: 2230 1156 2573 Fragments arranged by size: 2573 2230 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5959 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3690 4648 442 Size: 416 53 83 399 24 2273 958 1753 Fragments arranged by size: 2273 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5959 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2065 4619 1239 Size: 826 2554 2579 Fragments arranged by size: 2579 2554 826 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4084 4949 5247 4084 Size: 865 298 4796 Fragments arranged by size: 4796 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4622 882 Size: 617 3123 2219 Fragments arranged by size: 3123 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2758 2809 3750 493 Size: 711 1554 51 941 2702 Fragments arranged by size: 2702 1554 941 711 51 BsaBI GATnn'nnATC Cuts at: 5953 5953 Size: 5959 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3766 4148 4783 275 Size: 53 83 186 936 2233 382 635 1451 Fragments arranged by size: 2233 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2526 3132 4392 4790 5064 5211 5622 2526 Size: 606 1260 398 274 147 411 2863 Fragments arranged by size: 2863 1260 606 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2732 2762 2798 2828 1178 Size: 30 165 30 1329 30 36 30 4309 Fragments arranged by size: 4309 1329 165 36 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2494 2654 2740 2806 2975 1186 Size: 1308 160 86 66 169 4170 Fragments arranged by size: 4170 1308 169 160 86 66 BseYI C'CCAG_C Cuts at: 5162 5658 5162 Size: 496 5463 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3244 1163 Size: 573 1508 3878 Fragments arranged by size: 3878 1508 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3255 3409 4170 5198 1540 Size: 275 186 1254 154 761 1028 2301 Fragments arranged by size: 2301 1254 1028 761 275 186 154 BsiHKAI G_wGCw'C Cuts at: 2526 4020 4105 5176 2526 Size: 1494 85 1071 3309 Fragments arranged by size: 3309 1494 1071 85 BsmI GAATG_Cn' Cuts at: 2225 3470 3563 2225 Size: 1245 93 4621 Fragments arranged by size: 4621 1245 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2526 3278 4020 4105 5176 1391 Size: 399 736 752 742 85 1071 2174 Fragments arranged by size: 2174 1071 752 742 736 399 85 BspEI T'CCGG_A Cuts at: 5622 5622 Size: 5959 BspHI T'CATG_A Cuts at: 3848 5578 3848 Size: 1730 4229 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3195 3216 3314 844 Size: 2351 21 98 3489 Fragments arranged by size: 3489 2351 98 21 BsrBI CCG'CTC Cuts at: 1394 1823 3846 1394 Size: 429 2023 3507 Fragments arranged by size: 3507 2023 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2164 4454 4628 65 Size: 1183 916 2290 174 1396 Fragments arranged by size: 2290 1396 1183 916 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2697 4603 4790 1700 Size: 110 887 1906 187 2869 Fragments arranged by size: 2869 1906 887 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5959 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5959 BssSI C'ACGA_G Cuts at: 2957 3100 4019 5031 2957 Size: 143 919 1012 3885 Fragments arranged by size: 3885 1012 919 143 BstAPI GCAn_nnn'nTGC Cuts at: 794 3036 794 Size: 2242 3717 BstBI TT'CG_AA Cuts at: 4763 4763 Size: 5959 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5959 BstYI r'GATC_y Cuts at: 1972 2670 3289 3656 4041 4058 5510 5596 5608 Size: 698 619 367 385 17 1452 86 12 Cuts at: 5608 5954 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 698 619 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2182 2851 3255 513 Size: 552 1117 669 404 3217 Fragments arranged by size: 3217 1117 669 552 404 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2439 487 Size: 683 56 1213 4007 Fragments arranged by size: 4007 1213 683 56 BtsI GCAGTG_nn' Cuts at: 2977 3208 3475 4269 4289 2977 Size: 231 267 794 20 4647 Fragments arranged by size: 4647 794 267 231 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5959 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5924 DraI TTT'AAA Cuts at: 3263 3263 Size: 5959 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4966 809 Size: 507 3650 1802 Fragments arranged by size: 3650 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3252 3406 4295 Size: 54 1425 50 275 18 1422 154 889 Cuts at: 4295 5924 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1422 889 275 154 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3252 3406 1537 Size: 275 1440 154 4090 Fragments arranged by size: 4090 1440 275 154 EarI CTCTTCn'nnn_ Cuts at: 1353 2471 3889 1353 Size: 1118 1418 3423 Fragments arranged by size: 3423 1418 1118 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2917 3005 4546 4920 5066 387 Size: 1064 138 1328 88 1541 374 146 1280 Fragments arranged by size: 1541 1328 1280 1064 374 146 138 88 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2065 4022 4619 5406 5674 1239 Size: 393 433 1957 597 787 268 1524 Fragments arranged by size: 1957 1524 787 597 433 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2338 2834 2338 Size: 496 5463 EcoO109I rG'GnC_Cy Cuts at: 2604 2779 2839 2604 Size: 175 60 5724 Fragments arranged by size: 5724 175 60 EcoRI G'AATT_C Cuts at: 3268 3646 3268 Size: 378 5581 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2435 2435 Size: 5959 FspI TGC'GCA Cuts at: 2651 4465 2651 Size: 1814 4145 FspAI rTGC'GCAy Cuts at: 2651 2651 Size: 5959 HaeII r_GCGC'y Cuts at: 1678 2923 5106 1678 Size: 1245 2183 2531 Fragments arranged by size: 2531 2183 1245 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2100 2132 2732 2764 3083 Size: 32 135 32 528 32 600 32 319 Cuts at: 3083 3115 4600 4632 4674 4706 1373 Size: 32 1485 32 42 32 2626 Fragments arranged by size: 2626 1485 600 528 319 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3303 3549 669 Size: 988 281 1365 246 3079 Fragments arranged by size: 3079 1365 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5959 HpaI GTT'AAC Cuts at: 3549 3549 Size: 5959 KpnI G_GTAC'C Cuts at: 3284 3284 Size: 5959 MfeI C'AATT_G Cuts at: 3558 3558 Size: 5959 MluI A'CGCG_T Cuts at: 5835 5835 Size: 5959 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2285 3048 5073 5257 1305 Size: 980 763 2025 184 2007 Fragments arranged by size: 2025 2007 980 763 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4137 Fragments arranged by size: 4137 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3917 4276 4435 518 Size: 648 2751 359 159 2042 Fragments arranged by size: 2751 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2085 2853 3257 3375 4052 5200 5445 1606 Size: 479 768 404 118 677 1148 245 2120 Fragments arranged by size: 2120 1148 768 677 479 404 245 118 NcoI C'CATG_G Cuts at: 513 1065 2182 513 Size: 552 1117 4290 Fragments arranged by size: 4290 1117 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5959 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5959 NotI GC'GGCC_GC Cuts at: 3252 3406 3252 Size: 154 5805 NspI r_CATG'y Cuts at: 1132 1229 2950 3034 3052 3317 4862 1132 Size: 97 1721 84 18 265 1545 2229 Fragments arranged by size: 2229 1721 1545 265 97 84 18 PasI CC'CwG_GG Cuts at: 1348 2542 1348 Size: 1194 4765 PciI A'CATG_T Cuts at: 2946 4858 2946 Size: 1912 4047 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5959 PfoI T'CCnGG_A Cuts at: 2625 2625 Size: 5959 PmeI GTTT'AAAC Cuts at: 3263 3263 Size: 5959 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4028 4060 5541 5573 4028 Size: 32 1481 32 4414 Fragments arranged by size: 4414 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2779 2839 2779 Size: 60 5899 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5959 PsiI TTA'TAA Cuts at: 3529 3529 Size: 5959 PstI C_TGCA'G Cuts at: 830 2942 3311 830 Size: 2112 369 3478 Fragments arranged by size: 3478 2112 369 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5959 SacII CC_GC'GG Cuts at: 2854 3258 2854 Size: 404 5555 SalI G'TCGA_C Cuts at: 1936 3301 1936 Size: 1365 4594 SbfI CC_TGCA'GG Cuts at: 3311 3311 Size: 5959 ScaI AGT'ACT Cuts at: 4207 5919 4207 Size: 1712 4247 SfcI C'TryA_G Cuts at: 826 1045 2448 2938 3307 4442 5123 5314 826 Size: 219 1403 490 369 1135 681 191 1471 Fragments arranged by size: 1471 1403 1135 681 490 369 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5959 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5959 SmaI CCC'GGG Cuts at: 1872 2663 2885 3286 1872 Size: 791 222 401 4545 Fragments arranged by size: 4545 791 401 222 SmlI C'TyrA_G Cuts at: 820 1017 1943 4063 4964 5226 820 Size: 197 926 2120 901 262 1553 Fragments arranged by size: 2120 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3750 493 Size: 3257 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5959 SphI G_CATG'C Cuts at: 1132 3052 3317 1132 Size: 1920 265 3774 Fragments arranged by size: 3774 1920 265 SspI AAT'ATT Cuts at: 5 52 3883 5 Size: 47 3831 2081 Fragments arranged by size: 3831 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5959 StyI C'CwwG_G Cuts at: 513 1065 2182 2600 2769 2830 3398 513 Size: 552 1117 418 169 61 568 3074 Fragments arranged by size: 3074 1117 568 552 418 169 61 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2024 2037 3986 4003 4156 4341 4786 Size: 1255 444 13 1949 17 153 185 445 Cuts at: 4786 325 Size: 1498 Fragments arranged by size: 1949 1498 1255 445 444 185 153 17 13 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2934 4205 5917 Size: 274 80 33 51 1443 957 1271 1712 Cuts at: 5917 96 Size: 138 Fragments arranged by size: 1712 1443 1271 957 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5919 5951 5919 Size: 32 5927 Tth111I GACn'n_nGTC Cuts at: 1316 2240 1316 Size: 924 5035 XbaI T'CTAG_A Cuts at: 3295 3295 Size: 5959 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5959 XmaI C'CCGG_G Cuts at: 1870 2661 2883 3284 1870 Size: 791 222 401 4545 Fragments arranged by size: 4545 791 401 222 XmnI GAAnn'nnTTC Cuts at: 4088 4088 Size: 5959 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3767 276 Size: 53 83 186 936 2233 2468 Fragments arranged by size: 2468 2233 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI