################################## # NO CUT ENZYMES ( 123 enzymes) # ################################## AatII Acc65I AccIII AcsI AcyI AflII AgeI AhdI Alw44I AocI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI BalI BamHI BanI BanII BbeI BbrPI BbvCI BciVI BfrI BsaBI BsaHI BsaI BseAI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BsrBI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI Eam1105I Eco81I EcoO65I EcoRI EcoRV EcoT22I FseI Hin1I HincII HindII HindIII HpaI KasI KpnI MamI MfeI MluI MluNI MroI MscI MseI MslI MunI NaeI NarI NdeI NgoMIV NheI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI PshBI PvuI PvuII RcaI RsrII SacI SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tsp45I Van91I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AccI 238 GTCTAC GT^[AC][GT]AC AclI 258 AACGTT AA^CGTT AflIII 941 ACATGT A^C[GA][CT]GT AlwI 110 GGATC GGATC(4/5) AlwNI 1221 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 914 AGCGCT AGC^GCT AspHI 517 GAGCAC G[AT]GC[AT]^C AspI 232 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 644 TGCGCA TGC^GCA AvrII 825 CCTAGG C^CTAGG BbsI 358 GAAGAC GAAGAC(2/6) BcgI 480 GCAACCTACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 860 TGATCA T^GATCA BglI 845 GCCGCGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 665 AGATCT A^GATCT BlnI 825 CCTAGG C^CTAGG BlpI 409 GCTGAGC GC^T[ACGT]AGC BmyI 517 GAGCAC G[AGT]GC[ACT]^C BpmI 75 CTCCAG CTGGAG(16/14) Bpu1102I 409 GCTGAGC GC^T[ACGT]AGC BpuAI 358 GAAGAC GAAGAC(2/6) BsgI 1218 GTGCAG GTGCAG(16/14) BsiEI 1247 CGGCCG CG[GA][CT]^CG BsiHKAI 517 GAGCAC G[AT]GC[AT]^C BsmAI 1076 GAGAC GTCTC(1/5) BsmI 214 GAATGC GAATGC(1/-1) Bsp1286I 517 GAGCAC G[AGT]GC[ACT]^C BspLU11I 941 ACATGT A^CATGT BsrDI 152 GCAATG GCAATG(2/0) BsrFI 692 ACCGGC [GA]^CCGG[CT] BstAPI 1025 GCATGTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 665 AGATCT [GA]^GATC[CT] CelII 409 GCTGAGC GC^T[ACGT]AGC Cfr10I 692 ACCGGC [GA]^CCGG[CT] EaeI 1247 CGGCCG [CT]^GGCC[GA] EagI 1247 CGGCCG C^GGCCG EarI 471 GAAGAG CTCTTC(1/4) EclXI 1247 CGGCCG C^GGCCG Eco47III 914 AGCGCT AGC^GCT Eco52I 1247 CGGCCG C^GGCCG FbaI 860 TGATCA T^GATCA FspI 644 TGCGCA TGC^GCA HaeII 914 AGCGCT [GA]GCGC^[CT] Ksp632I 471 GAAGAG CTCTTC(1/4) KspI 846 CCGCGG CCGC^GG MaeIII 1178 GTTAC ^GT[ACGT]AC MflI 665 AGATCT [GA]^GATC[CT] NcoI 177 CCATGG C^CATGG NotI 1246 GCGGCCGC GC^GGCCGC PflFI 232 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC Psp1406I 258 AACGTT AA^CGTT PstI 933 CTGCAG CTGCA^G SacII 846 CCGCGG CCGC^GG SphI 1043 GCATGC GCATG^C Tsp509I 460 AATT ^AATT Tth111I 232 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoII 665 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### BfaI 826 CTAG C^TAG 1106 CTAG BsaAI 751 TACGTG [CT]AC^GT[GA] 802 TACGTG BsaWI 521 ACCGGA [AT]^CCGG[AT] 1127 TCCGGT BsmFI 938 GGGAC GGGAC(10/14) 775 GTCCC BspMI 1181 ACCTGC ACCTGC(4/8) 1220 GCAGGT BsrI 201 ACTGG ACTGG(1/-1) 269 ACTGG BssSI 952 CACGAG CACGAG(-5/-1) 1095 CTCGTG DsaI 177 CCATGG C^C[GA][CT]GG 846 CCGCGG EcoNI 329 CCTTTGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 825 CCTAGGCGAGG MaeI 826 CTAG C^TAG 1106 CTAG MspA1I 78 CAGCGG C[AC]G^C[GT]G 846 CCGCGG PpuMI 773 AGGTCCC [GA]G^G[AT]CC[CT] 833 AGGACCC SfcI 443 CTACAG C^T[GA][CT]AG 933 CTGCAG SmaI 656 CCCGGG CCC^GGG 878 CCCGGG XmaCI 656 CCCGGG C^CCGGG 878 CCCGGG XmaI 656 CCCGGG C^CCGGG 878 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 62 enzymes) # ######################################### AccII AciI AfaI AluI AvaI AvaII BbvI BcnI BsaJI BseRI BsiYI BslI BsoBI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraII EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI MaeII MboI MboII MnlI MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI RsaI Sau3AI Sau96I ScrFI SfaNI StyI TaqI TseI TspRI TthHB8I