MAS1 ORF clone pFN21AE1808

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Halotag ORF Clone pFN21AE1808

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Clone info.

Species

human

Clone Name

pFN21AE1808 Sequence (with vector) Restriction Map

Product ID

FHC07630

Flexi Clone PID

FXC07630

ORF Sequence

DNA sequence (975 bp)
Protein sequence (325 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE1808

Symbol

MAS1

Alias : MAS, MGRA

Description

MAS1 proto-oncogene, G protein-coupled receptor

Original Clone ID

cs02708

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 975 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 325 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS5272.1 2.4e-103 100.0 MAS1 CCDS7846.1 3.4e-26 38.3 MRGPRX1 CCDS7847.1 8.9e-26 37.7 MRGPRX2 CCDS7831.1 1.4e-25 37.3 MRGPRX4 CCDS31625.1 3.6e-25 34.0 MRGPRD The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR000820 21 37 PR00533 Mas transmembrane protein IPR000276 66 87 PR00237 GPCR IPR000820 85 101 PR00533 Mas transmembrane protein IPR000276 112 134 PR00237 GPCR IPR000276 148 169 PR00237 GPCR IPR000820 167 183 PR00533 Mas transmembrane protein IPR000820 212 228 PR00533 Mas transmembrane protein IPR000276 226 250 PR00237 GPCR IPR000820 245 262 PR00533 Mas transmembrane protein IPR000276 262 288 PR00237 GPCR IPR000820 291 306 PR00533 Mas transmembrane protein IPR000820 306 323 PR00533 Mas transmembrane protein HMMPfam IPR000276 50 280 PF00001 GPCR ProfileScan IPR017452 49 280 PS50262 GPCR
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 36 HWVIMSISPVGFVENGILLWFLC 58 PRIMARY 23 2 68 VYITHLSIADISLLFCIFILSID 90 PRIMARY 23 3 109 SVTFLFGYNTGLYLLTAISVERC 131 SECONDARY 23 4 149 SALVCALLWALSCLVTTMEYVMC 171 PRIMARY 23 5 189 IFIAILSFLVFTPLMLVSSTILV 211 PRIMARY 23 6 227 YIVIMVTIIIFLIFAMPMRLLYL 249 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_002368 325 165180 100.0 100.0 NP_671732 322 607227 38.3 81.2 NP_473371 330 607228 37.7 84.3 NP_001290544 330 607228 37.7 84.3 NP_473373 322 607230 37.3 90.5 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE1808 Download

>KIEE1808  975 bp
ATGGATGGGTCAAACGTGACATCATTTGTTGTTGAGGAACCCACGAACATCTCAACTGGC
AGGAACGCCTCAGTCGGGAATGCACATCGGCAAATCCCCATCGTGCACTGGGTCATTATG
AGCATCTCCCCAGTGGGGTTTGTTGAGAATGGGATTCTCCTCTGGTTCCTGTGCTTCCGG
ATGAGAAGAAATCCCTTCACTGTCTACATCACCCACCTGTCTATCGCAGACATCTCACTG
CTCTTCTGTATTTTCATCTTGTCTATCGACTATGCTTTAGATTATGAGCTTTCTTCTGGC
CATTACTACACAATTGTCACATTATCAGTGACTTTTCTGTTTGGCTACAACACGGGCCTC
TATCTGCTGACGGCCATTAGTGTGGAGAGGTGCCTGTCAGTCCTTTACCCCATCTGGTAC
CGATGCCATCGCCCCAAGTACCAGTCGGCATTGGTCTGTGCCCTTCTGTGGGCTCTTTCT
TGCTTGGTGACCACCATGGAGTATGTCATGTGCATCGACAGAGAAGAAGAGAGTCACTCT
CGGAATGACTGCCGAGCAGTCATCATCTTTATAGCCATCCTGAGCTTCCTGGTCTTCACG
CCCCTCATGCTGGTGTCCAGCACCATCTTGGTTGTGAAGATCCGGAAGAACACGTGGGCT
TCCCATTCCTCCAAGCTTTACATAGTCATCATGGTCACCATCATTATATTCCTCATCTTC
GCTATGCCCATGAGACTCCTTTACCTGCTGTACTATGAGTATTGGTCGACCTTTGGGAAC
CTACACCACATTTCCCTGCTCTTCTCCACAATCAACAGTAGCGCCAACCCTTTCATTTAC
TTCTTTGTGGGAAGCAGTAAGAAGAAGAGATTCAAGGAGTCCTTAAAGGTTGTTCTGACC
AGGGCTTTCAAAGATGAAATGCAACCTCGGCGCCAGAAAGACAATTGTAATACGGTCACA
GTTGAGACTGTCGTC

ORF Protein

Cloned ORF protein sequence for pFN21AE1808 Download

>KIEE1808  325 aa
MDGSNVTSFVVEEPTNISTGRNASVGNAHRQIPIVHWVIMSISPVGFVENGILLWFLCFR
MRRNPFTVYITHLSIADISLLFCIFILSIDYALDYELSSGHYYTIVTLSVTFLFGYNTGL
YLLTAISVERCLSVLYPIWYRCHRPKYQSALVCALLWALSCLVTTMEYVMCIDREEESHS
RNDCRAVIIFIAILSFLVFTPLMLVSSTILVVKIRKNTWASHSSKLYIVIMVTIIIFLIF
AMPMRLLYLLYYEYWSTFGNLHHISLLFSTINSSANPFIYFFVGSSKKKRFKESLKVVLT
RAFKDEMQPRRQKDNCNTVTVETVV

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE1808 Download

>pFN21AE1808  5680 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGATGGGTCAAACGTGACATCATTTGTTGTTGA
GGAACCCACGAACATCTCAACTGGCAGGAACGCCTCAGTCGGGAATGCACATCGGCAAAT
CCCCATCGTGCACTGGGTCATTATGAGCATCTCCCCAGTGGGGTTTGTTGAGAATGGGAT
TCTCCTCTGGTTCCTGTGCTTCCGGATGAGAAGAAATCCCTTCACTGTCTACATCACCCA
CCTGTCTATCGCAGACATCTCACTGCTCTTCTGTATTTTCATCTTGTCTATCGACTATGC
TTTAGATTATGAGCTTTCTTCTGGCCATTACTACACAATTGTCACATTATCAGTGACTTT
TCTGTTTGGCTACAACACGGGCCTCTATCTGCTGACGGCCATTAGTGTGGAGAGGTGCCT
GTCAGTCCTTTACCCCATCTGGTACCGATGCCATCGCCCCAAGTACCAGTCGGCATTGGT
CTGTGCCCTTCTGTGGGCTCTTTCTTGCTTGGTGACCACCATGGAGTATGTCATGTGCAT
CGACAGAGAAGAAGAGAGTCACTCTCGGAATGACTGCCGAGCAGTCATCATCTTTATAGC
CATCCTGAGCTTCCTGGTCTTCACGCCCCTCATGCTGGTGTCCAGCACCATCTTGGTTGT
GAAGATCCGGAAGAACACGTGGGCTTCCCATTCCTCCAAGCTTTACATAGTCATCATGGT
CACCATCATTATATTCCTCATCTTCGCTATGCCCATGAGACTCCTTTACCTGCTGTACTA
TGAGTATTGGTCGACCTTTGGGAACCTACACCACATTTCCCTGCTCTTCTCCACAATCAA
CAGTAGCGCCAACCCTTTCATTTACTTCTTTGTGGGAAGCAGTAAGAAGAAGAGATTCAA
GGAGTCCTTAAAGGTTGTTCTGACCAGGGCTTTCAAAGATGAAATGCAACCTCGGCGCCA
GAAAGACAATTGTAATACGGTCACAGTTGAGACTGTCGTCGTTTAAACGAATTCGGGCTC
GGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACA
AAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCC
TTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACA
ACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTT
GTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTT
CAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGT
AAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAG
TAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTT
TCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTT
TTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAA
TAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTT
TTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGAT
GCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAG
ATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTG
CTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATA
CACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGAT
GGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCC
AACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATG
GGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAAC
GACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACT
GGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAA
GTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCT
GGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCC
TCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGA
CAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAA
GCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAG
GGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAA
AGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATC
GACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCC
CTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCG
CCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTT
CGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACC
GCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGC
CACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAG
AGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCG
CTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAA
CCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAG
GATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACT
CACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAT
AGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAA
AAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAG
GTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTG
CCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATT
AAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGT
ACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT