Restriction map for
pFN21AE1808
(Linear) (Six-base) MAPSORT of: KIEE1808.seq Check: 9526 from: 1 to: 975
KIEE1808.orf
With 182 enzymes: SgfI *
July 8, 2015 10:32 ..
AccI GT'mk_AC
Cuts at: 0 203 766 975
Size: 203 563 209
Fragments arranged by size:
563 209 203
Acc65I G'GTAC_C
Cuts at: 0 416 975
Size: 416 559
AflIII A'CryG_T
Cuts at: 0 650 975
Size: 650 325
ApaLI G'TGCA_C
Cuts at: 0 103 975
Size: 103 872
BanI G'GyrC_C
Cuts at: 0 389 416 929 975
Size: 389 27 513 46
Fragments arranged by size:
513 389 46 27
BanII G_rGCy'C
Cuts at: 0 474 975
Size: 474 501
BbeI G_GCGC'C
Cuts at: 0 933 975
Size: 933 42
BbsI GAAGACnn'nnnn_
Cuts at: 0 585 975
Size: 585 390
Bme1580I G_kGCm'C
Cuts at: 0 107 462 975
Size: 107 355 513
Fragments arranged by size:
513 355 107
BmrI ACTGGGnnnn_n'
Cuts at: 0 117 124 975
Size: 117 7 851
Fragments arranged by size:
851 117 7
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 552 554 975
Size: 552 2 421
Fragments arranged by size:
552 421 2
Bpu10I CC'TnA_GC
Cuts at: 0 580 975
Size: 580 395
BsaAI yAC'GTr
Cuts at: 0 653 975
Size: 653 322
BsaHI Gr'CG_yC
Cuts at: 0 930 975
Size: 930 45
BsaWI w'CCGG_w
Cuts at: 0 176 641 975
Size: 176 465 334
Fragments arranged by size:
465 334 176
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 148 975
Size: 148 827
BsiHKAI G_wGCw'C
Cuts at: 0 107 975
Size: 107 868
BsmI GAATG_Cn'
Cuts at: 0 84 975
Size: 84 891
Bsp1286I G_dGCh'C
Cuts at: 0 107 462 474 975
Size: 107 355 12 501
Fragments arranged by size:
501 355 107 12
BspEI T'CCGG_A
Cuts at: 0 176 641 975
Size: 176 465 334
Fragments arranged by size:
465 334 176
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 752 975
Size: 752 223
BstEII G'GTnAC_C
Cuts at: 0 486 693 975
Size: 486 207 282
Fragments arranged by size:
486 282 207
BstXI CCAn_nnnn'nTGG
Cuts at: 0 381 975
Size: 381 594
BstYI r'GATC_y
Cuts at: 0 638 975
Size: 638 337
BtgI C'CryG_G
Cuts at: 0 494 975
Size: 494 481
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 412 975
Size: 412 563
BtsI GCAGTG_nn'
Cuts at: 0 235 975
Size: 235 740
EaeI y'GGCC_r
Cuts at: 0 297 371 975
Size: 297 74 604
Fragments arranged by size:
604 297 74
EarI CTCTTCn'nnn_
Cuts at: 0 247 521 805 859 975
Size: 247 274 284 54 116
Fragments arranged by size:
284 274 247 116 54
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 897 975
Size: 897 78
HaeII r_GCGC'y
Cuts at: 0 824 933 975
Size: 824 109 42
Fragments arranged by size:
824 109 42
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 174 206 545 577 748 780 975
Size: 174 32 339 32 171 32 195
Fragments arranged by size:
339 195 174 171 32 32 32
HincII GTy'rAC
Cuts at: 0 767 975
Size: 767 208
HindIII A'AGCT_T
Cuts at: 0 673 975
Size: 673 302
KasI G'GCGC_C
Cuts at: 0 929 975
Size: 929 46
KpnI G_GTAC'C
Cuts at: 0 420 975
Size: 420 555
MfeI C'AATT_G
Cuts at: 0 311 942 975
Size: 311 631 33
Fragments arranged by size:
631 311 33
MscI TGG'CCA
Cuts at: 0 299 975
Size: 299 676
MslI CAynn'nnrTG
Cuts at: 0 379 610 975
Size: 379 231 365
Fragments arranged by size:
379 365 231
NarI GG'CG_CC
Cuts at: 0 930 975
Size: 930 45
NcoI C'CATG_G
Cuts at: 0 494 975
Size: 494 481
PmlI CAC'GTG
Cuts at: 0 653 975
Size: 653 322
PshAI GACnn'nnGTC
Cuts at: 0 970 975
Size: 970 5
SalI G'TCGA_C
Cuts at: 0 765 975
Size: 765 210
SapI GCTCTTCn'nnn_
Cuts at: 0 247 805 975
Size: 247 558 170
Fragments arranged by size:
558 247 170
SfoI GGC'GCC
Cuts at: 0 931 975
Size: 931 44
StyI C'CwwG_G
Cuts at: 0 494 975
Size: 494 481
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 226 975
Size: 226 749
TatI w'GTAC_w
Cuts at: 0 749 975
Size: 749 226
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 775 807 975
Size: 775 32 168
Fragments arranged by size:
775 168 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 624 975
Size: 624 351
XmnI GAAnn'nnTTC
Cuts at: 0 868 975
Size: 868 107
Enzymes that do cut:
AccI Acc65I AflIII ApaLI BanI BanII BbeI BbsI
Bme1580I BmrI BplI Bpu10I BsaAI BsaHI BsaWI BseRI
BsiHKAI BsmI Bsp1286I BspEI BspMI BstEII BstXI BstYI
BtgI BtgZI BtsI EaeI EarI FalI HaeII Hin4I
HincII HindIII KasI KpnI MfeI MscI MslI NarI
NcoI PmlI PshAI SalI SapI SfoI StyI TaqII
TatI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII AclI AcuI AfeI AflII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI BmgBI BmtI BpmI
BpuEI BsaI BsaBI BsaXI BseYI BsgI BsiEI BsiWI
BsmBI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstZ17I Bsu36I ClaI CspCI DraI DraIII
DrdI EagI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FseI FspI FspAI HpaI MluI MmeI MspA1I
NaeI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII
SacI SacII SanDI SbfI ScaI SexAI SfcI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI Tth111I XbaI XhoI XmaI ZraI