(Circular) (Six-base) MAPSORT of: pFN21AE1808.seq Check: 1683 from: 1 to: 5680 pFN21AE1808 5680 bp With 182 enzymes: SgfI * December 28, 2011 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3490 278 Size: 53 83 186 936 1954 2468 Fragments arranged by size: 2468 1954 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2208 2771 3023 1937 Size: 271 563 252 4594 Fragments arranged by size: 4594 563 271 252 Acc65I G'GTAC_C Cuts at: 2421 3001 2421 Size: 580 5100 AclI AA'CG_TT Cuts at: 1563 3807 4180 1563 Size: 2244 373 3063 Fragments arranged by size: 3063 2244 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3743 5127 5395 1632 Size: 2111 1384 268 1917 Fragments arranged by size: 2111 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5483 AflIII A'CryG_T Cuts at: 1203 2655 4579 5556 1203 Size: 1452 1924 977 1327 Fragments arranged by size: 1924 1452 1327 977 AgeI A'CCGG_T Cuts at: 4511 4511 Size: 5680 AhdI GACnn_n'nnGTC Cuts at: 4409 4409 Size: 5680 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5680 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5680 AlwNI CAG_nnn'CTG Cuts at: 3423 4995 3423 Size: 1572 4108 ApaLI G'TGCA_C Cuts at: 2108 3737 4893 2108 Size: 1629 1156 2895 Fragments arranged by size: 2895 1629 1156 ApoI r'AATT_y Cuts at: 1493 2989 3215 3367 5476 1493 Size: 1496 226 152 2109 1697 Fragments arranged by size: 2109 1697 1496 226 152 AseI AT'TA_AT Cuts at: 160 1032 4234 160 Size: 872 3202 1606 Fragments arranged by size: 3202 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5680 AvaI C'yCGr_G Cuts at: 1870 1943 3005 1870 Size: 73 1062 4545 Fragments arranged by size: 4545 1062 73 BamHI G'GATC_C Cuts at: 3010 3377 3010 Size: 367 5313 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2394 2421 2934 3001 4456 Size: 325 217 627 607 27 513 67 1455 Cuts at: 4456 618 Size: 1842 Fragments arranged by size: 1842 1455 627 607 513 325 217 67 27 BanII G_rGCy'C Cuts at: 1391 2479 2999 1391 Size: 1088 520 4072 Fragments arranged by size: 4072 1088 520 BbeI G_GCGC'C Cuts at: 2938 2938 Size: 5680 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2590 928 Size: 971 691 4018 Fragments arranged by size: 4018 971 691 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3871 3905 1903 Size: 34 1934 34 3678 Fragments arranged by size: 3678 1934 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3572 4782 3572 Size: 1210 4470 BclI T'GATC_A Cuts at: 1548 1548 Size: 5680 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4291 136 Size: 107 122 71 631 605 2619 1525 Fragments arranged by size: 2619 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5675 5675 Size: 5680 BlpI GC'TnA_GC Cuts at: 3097 3097 Size: 5680 Bme1580I G_kGCm'C Cuts at: 1790 2112 2467 3741 4897 1790 Size: 322 355 1274 1156 2573 Fragments arranged by size: 2573 1274 1156 355 322 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5680 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2122 2129 3411 Size: 416 53 83 399 24 705 7 1282 Cuts at: 3411 4369 442 Size: 958 1753 Fragments arranged by size: 1753 1282 958 705 416 399 83 53 24 7 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5680 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2557 2559 2557 Size: 2 5678 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4340 1239 Size: 3101 2579 Bpu10I CC'TnA_GC Cuts at: 2585 2585 Size: 5680 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3805 4670 4968 3805 Size: 865 298 4517 Fragments arranged by size: 4517 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4343 882 Size: 617 2844 2219 Fragments arranged by size: 2844 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2658 3471 493 Size: 711 1454 813 2702 Fragments arranged by size: 2702 1454 813 711 BsaBI GATnn'nnATC Cuts at: 5674 5674 Size: 5680 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2935 3487 3869 4504 Size: 53 83 186 936 1402 552 382 635 Cuts at: 4504 275 Size: 1451 Fragments arranged by size: 1451 1402 936 635 552 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2181 2646 4113 4511 4785 4932 5343 2181 Size: 465 1467 398 274 147 411 2518 Fragments arranged by size: 2518 1467 465 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5455 Fragments arranged by size: 5455 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2153 1186 Size: 967 4713 BseYI C'CCAG_C Cuts at: 4883 5379 4883 Size: 496 5184 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5107 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3130 3891 4919 1540 Size: 275 186 1129 761 1028 2301 Fragments arranged by size: 2301 1129 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2112 3741 3826 4897 2112 Size: 1629 85 1071 2895 Fragments arranged by size: 2895 1629 1071 85 BsmI GAATG_Cn' Cuts at: 2089 3191 3284 2089 Size: 1102 93 4485 Fragments arranged by size: 4485 1102 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2112 2467 2479 2999 3741 3826 4897 Size: 399 322 355 12 520 742 85 1071 Cuts at: 4897 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 520 399 355 322 85 12 BspEI T'CCGG_A Cuts at: 2181 2646 5343 2181 Size: 465 2697 2518 Fragments arranged by size: 2697 2518 465 BspHI T'CATG_A Cuts at: 3569 5299 3569 Size: 1730 3950 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2757 3035 844 Size: 1913 278 3489 Fragments arranged by size: 3489 1913 278 BsrBI CCG'CTC Cuts at: 1394 1823 3567 1394 Size: 429 1744 3507 Fragments arranged by size: 3507 1744 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4175 4349 65 Size: 1183 2927 174 1396 Fragments arranged by size: 2927 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4324 4511 1700 Size: 110 2514 187 2869 Fragments arranged by size: 2869 2514 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5680 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5680 BssSI C'ACGA_G Cuts at: 3740 4752 3740 Size: 1012 4668 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5680 BstBI TT'CG_AA Cuts at: 4484 4484 Size: 5680 BstEII G'GTnAC_C Cuts at: 1184 2491 2698 1184 Size: 1307 207 4166 Fragments arranged by size: 4166 1307 207 BstXI CCAn_nnnn'nTGG Cuts at: 2386 2386 Size: 5680 BstYI r'GATC_y Cuts at: 1972 2643 3010 3377 3762 3779 5231 5317 5329 Size: 671 367 367 385 17 1452 86 12 Cuts at: 5329 5675 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 671 385 367 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2499 513 Size: 552 939 495 3694 Fragments arranged by size: 3694 939 552 495 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2417 487 Size: 683 56 1191 3750 Fragments arranged by size: 3750 1191 683 56 BtsI GCAGTG_nn' Cuts at: 2240 3196 3990 4010 2240 Size: 956 794 20 3910 Fragments arranged by size: 3910 956 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5680 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5645 DraI TTT'AAA Cuts at: 2984 2984 Size: 5680 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4687 809 Size: 507 3371 1802 Fragments arranged by size: 3371 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2302 2376 3127 Size: 54 1425 50 275 18 472 74 751 Cuts at: 3127 4016 5645 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 751 472 275 74 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3127 1537 Size: 275 1315 4090 Fragments arranged by size: 4090 1315 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2252 2526 2810 2864 3610 1353 Size: 899 274 284 54 746 3423 Fragments arranged by size: 3423 899 746 284 274 54 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4267 4641 4787 387 Size: 1064 138 2678 374 146 1280 Fragments arranged by size: 2678 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3743 4340 5127 5395 1239 Size: 393 2111 597 787 268 1524 Fragments arranged by size: 2111 1524 787 597 393 268 EcoRI G'AATT_C Cuts at: 2989 3367 2989 Size: 378 5302 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2902 2902 Size: 5680 FspI TGC'GCA Cuts at: 4186 4186 Size: 5680 HaeII r_GCGC'y Cuts at: 1678 2829 2938 4827 1678 Size: 1151 109 1889 2531 Fragments arranged by size: 2531 1889 1151 109 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2179 2211 2550 2582 2753 Size: 32 135 32 607 32 339 32 171 Cuts at: 2753 2785 4321 4353 4395 4427 1373 Size: 32 1536 32 42 32 2626 Fragments arranged by size: 2626 1536 607 339 171 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2772 3024 3270 669 Size: 988 281 834 252 246 3079 Fragments arranged by size: 3079 988 834 281 252 246 HindIII A'AGCT_T Cuts at: 748 2678 748 Size: 1930 3750 HpaI GTT'AAC Cuts at: 3270 3270 Size: 5680 KasI G'GCGC_C Cuts at: 2934 2934 Size: 5680 KpnI G_GTAC'C Cuts at: 2425 3005 2425 Size: 580 5100 MfeI C'AATT_G Cuts at: 2316 2947 3279 2316 Size: 631 332 4717 Fragments arranged by size: 4717 631 332 MluI A'CGCG_T Cuts at: 5556 5556 Size: 5680 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4794 4978 1305 Size: 3489 184 2007 Fragments arranged by size: 3489 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2304 10 Size: 54 1425 343 472 3386 Fragments arranged by size: 3386 1425 472 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2384 2615 3638 3997 4156 518 Size: 648 1218 231 1023 359 159 2042 Fragments arranged by size: 2042 1218 1023 648 359 231 159 MspA1I CmG'CkG Cuts at: 1606 3096 3773 4921 5166 1606 Size: 1490 677 1148 245 2120 Fragments arranged by size: 2120 1490 1148 677 245 NarI GG'CG_CC Cuts at: 2935 2935 Size: 5680 NcoI C'CATG_G Cuts at: 513 1065 2004 2499 513 Size: 552 939 495 3694 Fragments arranged by size: 3694 939 552 495 NdeI CA'TA_TG Cuts at: 387 387 Size: 5680 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5680 NotI GC'GGCC_GC Cuts at: 3127 3127 Size: 5680 NspI r_CATG'y Cuts at: 1132 1229 3038 4583 1132 Size: 97 1809 1545 2229 Fragments arranged by size: 2229 1809 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5680 PciI A'CATG_T Cuts at: 4579 4579 Size: 5680 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5680 PmeI GTTT'AAAC Cuts at: 2984 2984 Size: 5680 PmlI CAC'GTG Cuts at: 2658 2658 Size: 5680 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3749 3781 5262 5294 3749 Size: 32 1481 32 4135 Fragments arranged by size: 4135 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2975 1613 Size: 1362 4318 PsiI TTA'TAA Cuts at: 3250 3250 Size: 5680 PstI C_TGCA'G Cuts at: 830 3032 830 Size: 2202 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5680 SalI G'TCGA_C Cuts at: 1936 2770 3022 1936 Size: 834 252 4594 Fragments arranged by size: 4594 834 252 SapI GCTCTTCn'nnn_ Cuts at: 2252 2810 2252 Size: 558 5122 SbfI CC_TGCA'GG Cuts at: 3032 3032 Size: 5680 ScaI AGT'ACT Cuts at: 3928 5640 3928 Size: 1712 3968 SfcI C'TryA_G Cuts at: 826 1045 3028 4163 4844 5035 826 Size: 219 1983 1135 681 191 1471 Fragments arranged by size: 1983 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2936 2936 Size: 5680 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5680 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5680 SmaI CCC'GGG Cuts at: 1872 3007 1872 Size: 1135 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3784 4685 4947 820 Size: 197 926 1841 901 262 1553 Fragments arranged by size: 1841 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3471 493 Size: 2978 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5680 SphI G_CATG'C Cuts at: 1132 3038 1132 Size: 1906 3774 SspI AAT'ATT Cuts at: 5 52 3604 5 Size: 47 3552 2081 Fragments arranged by size: 3552 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5680 StyI C'CwwG_G Cuts at: 513 1065 2004 2499 3119 513 Size: 552 939 495 620 3074 Fragments arranged by size: 3074 939 620 552 495 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2231 3707 3724 3877 4062 4507 325 Size: 1255 651 1476 17 153 185 445 1498 Fragments arranged by size: 1498 1476 1255 651 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2754 3926 5638 Size: 274 80 33 51 1443 777 1172 1712 Cuts at: 5638 96 Size: 138 Fragments arranged by size: 1712 1443 1172 777 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2780 2812 5640 5672 2780 Size: 32 2828 32 2788 Fragments arranged by size: 2828 2788 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5680 XbaI T'CTAG_A Cuts at: 3016 3016 Size: 5680 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2629 2629 Size: 5680 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5680 XmaI C'CCGG_G Cuts at: 1870 3005 1870 Size: 1135 4545 XmnI GAAnn'nnTTC Cuts at: 2873 3809 2873 Size: 936 4744 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3488 276 Size: 53 83 186 936 1954 2468 Fragments arranged by size: 2468 1954 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI