Restriction map for
pFN21AE1808
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# NO CUT ENZYMES ( 145 enzymes) #
##################################
AatII AccII AciI AclI AcsI
AflII AgeI AhdI AlwNI AocI
Aor51HI ApaI ApoI AscI AseI
AsnI AspEI AspI AvaI AvaII
AviII AvrII BamHI BbvCI BbvI
BcgI BciVI BclI BcnI BfaI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BsaBI BsaI BsgI
BsiEI BsiWI BsmBI BsmFI BsoBI
Bsp1407I BspDI BspHI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstUI BstZ17I
Bsu36I Cac8I CelII Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoRI EcoRV
EcoT22I FauI FbaI Fnu4HI FseI
FspI HgaI HpaI ItaI KspI
MaeI MamI MluI MspA1I MvnI
NaeI NciI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmeI PpuMI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SbfI ScaI SexAI SfcI
SfiI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TseI Tth111I
Van91I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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Acc65I 416 GGTACC G^GTACC
AcyI 929 GGCGCC G[GA]^CG[CT]C
AflIII 650 ACACGT A^C[GA][CT]GT
Alw44I 103 GTGCAC G^TGCAC
AlwI 639 GATCC GGATC(4/5)
ApaLI 103 GTGCAC G^TGCAC
Asp700I 864 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 416 GGTACC G^GTACC
AspHI 103 GTGCAC G[AT]GC[AT]^C
BalI 297 TGGCCA TGG^CCA
BanII 470 GGGCTC G[GA]GC[CT]^C
BbeI 929 GGCGCC GGCGC^C
BbrPI 651 CACGTG CAC^GTG
BbsI 592 GTCTTC GAAGAC(2/6)
BpuAI 592 GTCTTC GAAGAC(2/6)
BsaAI 651 CACGTG [CT]AC^GT[GA]
BsaHI 929 GGCGCC G[GA]^CG[CT]C
BseRI 157 CTCCTC GAGGAG(10/8)
BsiHKAI 103 GTGCAC G[AT]GC[AT]^C
BsmI 78 GAATGC GAATGC(1/-1)
BspMI 743 ACCTGC ACCTGC(4/8)
BstXI 374 CCATTAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 638 AGATCC [GA]^GATC[CT]
Cfr13I 354 GGGCC G^G[ACGT]CC
DpnII 639 GATC ^GATC
DsaI 494 CCATGG C^C[GA][CT]GG
EcoT14I 494 CCATGG C^C[AT][AT]GG
Hin1I 929 GGCGCC G[GA]^CG[CT]C
HincII 765 GTCGAC GT[CT]^[GA]AC
HindII 765 GTCGAC GT[CT]^[GA]AC
HindIII 673 AAGCTT A^AGCTT
KasI 929 GGCGCC G^GCGCC
KpnI 416 GGTACC GGTAC^C
MboI 639 GATC ^GATC
MflI 638 AGATCC [GA]^GATC[CT]
MluNI 297 TGGCCA TGG^CCA
MscI 297 TGGCCA TGG^CCA
MseI 883 TTAA T^TAA
NarI 929 GGCGCC GG^CGCC
NcoI 494 CCATGG C^CATGG
NdeII 639 GATC ^GATC
PmaCI 651 CACGTG CAC^GTG
PmlI 651 CACGTG CAC^GTG
PshAI 966 GACTGTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI 765 GTCGAC G^TCGAC
Sau3AI 639 GATC ^GATC
Sau96I 354 GGGCC G^G[ACGT]CC
SfoI 929 GGCGCC GGC^GCC
StyI 494 CCATGG C^C[AT][AT]GG
Tru9I 883 TTAA T^TAA
XcmI 617 CCAGCACCATCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 638 AGATCC [GA]^GATC[CT]
XmnI 864 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccI 202 GTCTAC GT^[AC][GT]AC
765 GTCGAC
AccIII 176 TCCGGA T^CCGGA
641 TCCGGA
BsaWI 176 TCCGGA [AT]^CCGG[AT]
641 TCCGGA
BseAI 176 TCCGGA T^CCGGA
641 TCCGGA
BsmAI 732 GAGAC GTCTC(1/5)
964 GAGAC
BspEI 176 TCCGGA T^CCGGA
641 TCCGGA
BssKI 588 CCTGG ^CC[ACGT]GG
899 CCAGG
BstEII 486 GGTGACC G^GT[ACGT]ACC
693 GGTCACC
BstNI 588 CCTGG CC^[AT]GG
899 CCAGG
BstPI 486 GGTGACC G^GT[ACGT]ACC
693 GGTCACC
CfoI 821 GCGC GCG^C
930 GCGC
DdeI 69 CTCAG C^T[ACGT]AG
580 CTGAG
EaeI 297 TGGCCA [CT]^GGCC[GA]
371 CGGCCA
EcoO65I 486 GGTGACC G^GT[ACGT]ACC
693 GGTCACC
EcoRII 588 CCTGG ^CC[AT]GG
899 CCAGG
HaeII 820 AGCGCC [GA]GCGC^[CT]
929 GGCGCC
HapII 177 CCGG C^CGG
642 CCGG
HhaI 821 GCGC GCG^C
930 GCGC
HinP1I 821 GCGC G^CGC
930 GCGC
HpaII 177 CCGG C^CGG
642 CCGG
MaeII 14 ACGT A^CGT
652 ACGT
MfeI 311 CAATTG C^AATTG
942 CAATTG
MroI 176 TCCGGA T^CCGGA
641 TCCGGA
MslI 375 CATTAGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
606 CATGCTGGTG
MspI 177 CCGG C^CGG
642 CCGG
MunI 311 CAATTG C^AATTG
942 CAATTG
MvaI 588 CCTGG CC^[AT]GG
899 CCAGG
MwoI 600 GCCCCTCATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
921 GCAACCTCGGC
SapI 240 GCTCTTC GCTCTTC(1/4)
798 GCTCTTC
ScrFI 588 CCTGG CC^[ACGT]GG
899 CCAGG
TfiI 153 GATTC G^A[AT]TC
869 GATTC
Tsp509I 312 AATT ^AATT
943 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AfaI AluI BanI BmyI BsaJI
BsiYI BslI Bsp1286I BsrI BstF5I
EarI FokI HaeIII HinfI HphI
Ksp632I MaeIII MboII MnlI NlaIII
NlaIV PleI RsaI SfaNI TaqI
Tsp45I TspRI TthHB8I