RUSC1 ORF clone pFN21AE1122

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE1122

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Clone info.

Species

human

Clone Name

pFN21AE1122 Sequence (with vector) Restriction Map

Product ID

FHC07945

ORF Sequence

DNA sequence (1299 bp)
Protein sequence (433 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB590126]

Gene

KIEE1122

Symbol

RUSC1

Alias : NESCA

Description

RUN and SH3 domain containing 1, transcript variant 4

Original Clone ID

oc10019

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1299 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 433 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS1112.1 6.1e-112 100.0 RUSC1 CCDS41412.1 6.7e-112 100.0 RUSC1 CCDS41410.1 1.1e-111 100.0 RUSC1 CCDS41411.1 7.9e-44 75.5 RUSC1 CCDS35008.1 1.8e-20 39.8 RUSC2 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR004012 61 194 PF02759 RUN IPR001452 381 426 PF00018 Src homology-3 domain HMMSmart IPR004012 127 195 SM00593 RUN IPR001452 378 433 SM00326 Src homology-3 domain ProfileScan IPR004012 53 197 PS50826 RUN IPR001452 375 433 PS50002 Src homology-3 domain
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_001317669 638 611053 39.8 82.7 NP_055621 1516 611053 39.8 82.7 NP_001129471 1516 611053 39.8 82.7 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE1122 Download

>KIEE1122  1299 bp
ATGGCAGAAGCCCAGAGTGGGACTGGTCAGCTGCAGGAGCAGAAGAAAGGTCTTCTGATA
GCCGTCAGCGTCTCCGTTGATAAAATCATCTCGCATTTCGGGGCCGCCCGGAACTTGGTG
CAGAAGGCCCAGTTGGGTGATAGCCGGCTGAGCCCGGATGTGGGGCACCTGGTGCTGACC
ACCCTCTGCCCGGCCCTCCACGCCCTGGTGGCGGACGGGCTGAAGCCTTTCCGGAAGGAC
CTCATCACCGGGCAGCGCAGGAGCAGCCCCTGGAGCGTGGTGGAGGCGTCGGTGAAGCCA
GGCTCCAGCACCCGCTCCCTTGGAACCCTGTATAGCCAGGTCAGCCGTCTAGCCCCGCTG
AGCAGCAGCCGTAGCCGCTTCCATGCCTTTATCCTGGGCCTCCTCAACACCAAGCAGTTG
GAGCTGTGGTTTTCCAGTCTCCAGGAAGATGCAGGCCTGCTCTCCCTCCTGTACCTGCCA
ACAGGATTTTTCTCCCTGGCCCGCGGTGGTTGTCCCTCCCTGTCCACAGAGCTGCTGCTC
CTGCTGCAGCCATTGTCGGTGCTCACTTTCCACCTGGACCTGCTCTTTGAGCACCACCAC
CACCTGCCCCTGGGCCCACCTCAGGCCCCTGCCCCTCCAGGCCCACCTCCAGCTCTGCAG
CAGACTATGCAAGCCATGCTGCACTTTGGGGGCCGGCTGGCCCAGAGCCTTCGGGGGACT
TCCAAGGAAGCTGCTTCAGACCCCTCTGACTCTCCAAACCTTCCCACACCAGGGAGCTGG
TGGGAGCAGTTGACCCAGGCCTCCCGGGTCTATGCCTCTGGGGGCACTGAGGGCTTTCCT
CTTTCCCGATGGGCACCGGGGCGTCATGGGACTGCAGCTGAAGAAGGTGCACAGGAGAGA
CCCCTGCCCACAGATGAGATGGCACCAGGCAGGGGCCTCTGGTTGGGAAGACTATTTGGA
GTGCCTGGGGGCCCCGCAGAAAATGAGAATGGAGCCCTAAAGTCCAGGAGACCATCTAGC
TGGCTGCCCCCGACAGTGAGTGTGTTGGCTCTTGTGAAGCGGGGGGCACCTCCCGAGATG
CCTTCTCCTCAGGAGCTTGAGGCCTCAGCACCCAGGATGGTGCAAACCCATAGGGCAGTG
CGGGCTCTCTGTGATCACACTGCTGCAAGACCTGACCAGTTGAGCTTCCGGCGTGGGGAA
GTGCTGCGTGTCATCACCACAGTGGATGAGGACTGGCTCCGCTGTGGGCGGGATGGCATG
GAGGGTCTGGTGCCTGTGGGGTATACCTCCCTTGTTCTG

ORF Protein

Cloned ORF protein sequence for pFN21AE1122 Download

>KIEE1122  433 aa
MAEAQSGTGQLQEQKKGLLIAVSVSVDKIISHFGAARNLVQKAQLGDSRLSPDVGHLVLT
TLCPALHALVADGLKPFRKDLITGQRRSSPWSVVEASVKPGSSTRSLGTLYSQVSRLAPL
SSSRSRFHAFILGLLNTKQLELWFSSLQEDAGLLSLLYLPTGFFSLARGGCPSLSTELLL
LLQPLSVLTFHLDLLFEHHHHLPLGPPQAPAPPGPPPALQQTMQAMLHFGGRLAQSLRGT
SKEAASDPSDSPNLPTPGSWWEQLTQASRVYASGGTEGFPLSRWAPGRHGTAAEEGAQER
PLPTDEMAPGRGLWLGRLFGVPGGPAENENGALKSRRPSSWLPPTVSVLALVKRGAPPEM
PSPQELEASAPRMVQTHRAVRALCDHTAARPDQLSFRRGEVLRVITTVDEDWLRCGRDGM
EGLVPVGYTSLVL

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE1122 Download

>pFN21AE1122  6004 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCAGAAGCCCAGAGTGGGACTGGTCAGCTGCA
GGAGCAGAAGAAAGGTCTTCTGATAGCCGTCAGCGTCTCCGTTGATAAAATCATCTCGCA
TTTCGGGGCCGCCCGGAACTTGGTGCAGAAGGCCCAGTTGGGTGATAGCCGGCTGAGCCC
GGATGTGGGGCACCTGGTGCTGACCACCCTCTGCCCGGCCCTCCACGCCCTGGTGGCGGA
CGGGCTGAAGCCTTTCCGGAAGGACCTCATCACCGGGCAGCGCAGGAGCAGCCCCTGGAG
CGTGGTGGAGGCGTCGGTGAAGCCAGGCTCCAGCACCCGCTCCCTTGGAACCCTGTATAG
CCAGGTCAGCCGTCTAGCCCCGCTGAGCAGCAGCCGTAGCCGCTTCCATGCCTTTATCCT
GGGCCTCCTCAACACCAAGCAGTTGGAGCTGTGGTTTTCCAGTCTCCAGGAAGATGCAGG
CCTGCTCTCCCTCCTGTACCTGCCAACAGGATTTTTCTCCCTGGCCCGCGGTGGTTGTCC
CTCCCTGTCCACAGAGCTGCTGCTCCTGCTGCAGCCATTGTCGGTGCTCACTTTCCACCT
GGACCTGCTCTTTGAGCACCACCACCACCTGCCCCTGGGCCCACCTCAGGCCCCTGCCCC
TCCAGGCCCACCTCCAGCTCTGCAGCAGACTATGCAAGCCATGCTGCACTTTGGGGGCCG
GCTGGCCCAGAGCCTTCGGGGGACTTCCAAGGAAGCTGCTTCAGACCCCTCTGACTCTCC
AAACCTTCCCACACCAGGGAGCTGGTGGGAGCAGTTGACCCAGGCCTCCCGGGTCTATGC
CTCTGGGGGCACTGAGGGCTTTCCTCTTTCCCGATGGGCACCGGGGCGTCATGGGACTGC
AGCTGAAGAAGGTGCACAGGAGAGACCCCTGCCCACAGATGAGATGGCACCAGGCAGGGG
CCTCTGGTTGGGAAGACTATTTGGAGTGCCTGGGGGCCCCGCAGAAAATGAGAATGGAGC
CCTAAAGTCCAGGAGACCATCTAGCTGGCTGCCCCCGACAGTGAGTGTGTTGGCTCTTGT
GAAGCGGGGGGCACCTCCCGAGATGCCTTCTCCTCAGGAGCTTGAGGCCTCAGCACCCAG
GATGGTGCAAACCCATAGGGCAGTGCGGGCTCTCTGTGATCACACTGCTGCAAGACCTGA
CCAGTTGAGCTTCCGGCGTGGGGAAGTGCTGCGTGTCATCACCACAGTGGATGAGGACTG
GCTCCGCTGTGGGCGGGATGGCATGGAGGGTCTGGTGCCTGTGGGGTATACCTCCCTTGT
TCTGGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCA
TGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCG
CTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATAC
ATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAA
ATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAAC
AACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGC
AAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCG
TTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGC
GGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTG
CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGA
CAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACAT
TTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCA
GAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATC
GAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCA
ATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGG
CAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCA
GTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATA
ACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAG
CTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCG
GAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCA
ACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTA
ATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCT
GGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCA
GCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAG
GCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCAT
TGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGG
TAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCC
AGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCC
CCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGAC
TATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCC
TGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATA
GCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGC
ACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCA
ACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAG
CGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTA
GAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTG
GTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGC
AGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGT
CTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAA
GGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCT
TCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGT
GGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGC
GGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAA
CGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTC
GTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAG
ATCT