Restriction map for
pFN21AE1122
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# NO CUT ENZYMES ( 127 enzymes) #
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AatII Acc65I AclI AcsI AflII
AflIII AgeI AhdI AlwI Aor51HI
ApoI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BalI
BamHI BbeI BbrPI BcgI BciVI
BfrI BglII BlnI BsaAI BsaBI
BsiEI BsiWI BsmI Bsp1407I BspDI
BspHI BspLU11I BsrDI BsrGI BssHII
BssSI BstBI BstEII BstPI BstYI
ClaI CpoI DraI DrdI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FseI FspI HaeII HindIII
HpaI KasI KpnI Ksp632I MaeII
MaeIII MamI MfeI MflI MluI
MluNI MscI MseI MunI NarI
NcoI NdeI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SalI SapI SbfI
ScaI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI TaqI TfiI Tru9I
Tsp45I Tsp509I Tth111I TthHB8I XbaI
XhoI XhoII
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccI 1281 GTATAC GT^[AC][GT]AC
AccII 502 CGCG CG^CG
AccIII 230 TCCGGA T^CCGGA
AfaI 471 GTAC GT^AC
Alw44I 887 GTGCAC G^TGCAC
AlwNI 264 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 887 GTGCAC G^TGCAC
Asp700I 222 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbvCI 1103 CCTCAGC CCTCAGC(-5/-2)
BclI 1152 TGATCA T^GATCA
BglI 202 GCCCTGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 230 TCCGGA [AT]^CCGG[AT]
BseAI 230 TCCGGA T^CCGGA
BsmBI 69 CGTCTC CGTCTC(1/5)
BspEI 230 TCCGGA T^CCGGA
BsrBI 312 CCGCTC CCGCTC(-3/-3)
Bst1107I 1281 GTATAC GTA^TAC
BstUI 502 CGCG CG^CG
BstZ17I 1281 GTATAC GTA^TAC
CfoI 255 GCGC GCG^C
DpnII 1153 GATC ^GATC
DraIII 166 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 501 CCGCGG C^C[GA][CT]GG
FbaI 1152 TGATCA T^GATCA
HhaI 255 GCGC GCG^C
HinP1I 255 GCGC G^CGC
HincII 789 GTTGAC GT[CT]^[GA]AC
HindII 789 GTTGAC GT[CT]^[GA]AC
HinfI 748 GACTC G^A[ACGT]TC
KspI 501 CCGCGG CCGC^GG
MboI 1153 GATC ^GATC
MroI 230 TCCGGA T^CCGGA
MslI 1215 CACCACAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 502 CGCG CG^CG
NdeII 1153 GATC ^GATC
PflMI 198 CCACGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 748 GACTC GAGTC(4/5)
PpuMI 236 AGGACCT [GA]G^G[AT]CC[CT]
RsaI 471 GTAC GT^AC
SacII 501 CCGCGG CCGC^GG
Sau3AI 1153 GATC ^GATC
SexAI 167 ACCTGGT A^CC[AT]GGT
SmaI 803 CCCGGG CCC^GGG
SmlI 1096 CTTGAG C^T[CT][GA]AG
Van91I 198 CCACGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 803 CCCGGG C^CCGGG
XmaI 803 CCCGGG C^CCGGG
XmnI 222 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 28 enzymes) #
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AcyI 285 GGCGTC G[GA]^CG[CT]C
860 GGCGTC
AocI 619 CCTCAGG CC^T[ACGT]AGG
1087 CCTCAGG
ApaI 612 GGGCCC GGGCC^C
969 GGGCCC
AvaI 803 CCCGGG C^[CT]CG[GA]G
1072 CCCGAG
AvaII 237 GGACC G^G[AT]CC
576 GGACC
BbsI 947 GAAGAC GAAGAC(2/6)
50 GTCTTC
BfaI 349 CTAG C^TAG
1016 CTAG
BlpI 147 GCTGAGC GC^T[ACGT]AGC
357 GCTGAGC
Bpu1102I 147 GCTGAGC GC^T[ACGT]AGC
357 GCTGAGC
BpuAI 947 GAAGAC GAAGAC(2/6)
50 GTCTTC
BsaHI 285 GGCGTC G[GA]^CG[CT]C
860 GGCGTC
BsaI 897 GAGACC GGTCTC(1/5)
1008 GAGACC
BseRI 400 CTCCTC GAGGAG(10/8)
1085 CTCCTC
BsgI 679 CTGCAC GTGCAG(16/14)
118 GTGCAG
BsoBI 803 CCCGGG C^[CT]CG[GA]G
1072 CCCGAG
BsrFI 143 GCCGGC [GA]^CCGG[CT]
692 GCCGGC
Bsu36I 619 CCTCAGG CC^T[ACGT]AGG
1087 CCTCAGG
CelII 147 GCTGAGC GC^T[ACGT]AGC
357 GCTGAGC
Cfr10I 143 GCCGGC [GA]^CCGG[CT]
692 GCCGGC
Eco81I 619 CCTCAGG CC^T[ACGT]AGG
1087 CCTCAGG
EcoT14I 318 CCTTGG C^C[AT][AT]GG
722 CCAAGG
Hin1I 285 GGCGTC G[GA]^CG[CT]C
860 GGCGTC
MaeI 349 CTAG C^TAG
1016 CTAG
NaeI 143 GCCGGC GCC^GGC
692 GCCGGC
NgoMIV 143 GCCGGC G^CCGGC
692 GCCGGC
PvuII 28 CAGCTG CAG^CTG
875 CAGCTG
SfaNI 448 GATGC GCATC(5/9)
1077 GATGC
StyI 318 CCTTGG C^C[AT][AT]GG
722 CCAAGG
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# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AluI AspHI BanI BanII
BbvI BcnI BmyI BpmI BsaJI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrI BssKI BstAPI
BstF5I BstNI BstXI Cac8I Cfr13I
DdeI DraII EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HgaI
HpaII HphI ItaI MboII MnlI
MspA1I MspI MvaI MwoI NciI
NlaIII NlaIV PstI Sau96I ScrFI
SfcI StuI TseI TspRI XcmI