(Circular) (Six-base) MAPSORT of: pFN21AE1122.seq Check: 3155 from: 1 to: 6004 pFN21AE1122 6004 bp With 182 enzymes: SgfI * August 16, 2010 14:43 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2616 2616 Size: 6004 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3814 278 Size: 53 83 186 936 2278 2468 Fragments arranged by size: 2468 2278 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3287 3347 1937 Size: 1350 60 4594 Fragments arranged by size: 4594 1350 60 Acc65I G'GTAC_C Cuts at: 3325 3325 Size: 6004 AclI AA'CG_TT Cuts at: 1563 4131 4504 1563 Size: 2568 373 3063 Fragments arranged by size: 3063 2568 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2246 2724 2904 4067 5451 5719 1632 Size: 614 478 180 1163 1384 268 1917 Fragments arranged by size: 1917 1384 1163 614 478 268 180 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5807 AflIII A'CryG_T Cuts at: 1203 4903 5880 1203 Size: 3700 977 1327 Fragments arranged by size: 3700 1327 977 AgeI A'CCGG_T Cuts at: 4835 4835 Size: 6004 AhdI GACnn_n'nnGTC Cuts at: 4733 4733 Size: 6004 AleI CACnn'nnGTG Cuts at: 1166 3224 1166 Size: 2058 3946 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6004 AlwNI CAG_nnn'CTG Cuts at: 2274 3747 5319 2274 Size: 1473 1572 2959 Fragments arranged by size: 2959 1572 1473 ApaI G_GGCC'C Cuts at: 2621 2978 2621 Size: 357 5647 ApaLI G'TGCA_C Cuts at: 2892 4061 5217 2892 Size: 1169 1156 3679 Fragments arranged by size: 3679 1169 1156 ApoI r'AATT_y Cuts at: 1493 3313 3539 3691 5800 1493 Size: 1820 226 152 2109 1697 Fragments arranged by size: 2109 1820 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4558 160 Size: 872 3526 1606 Fragments arranged by size: 3526 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6004 AvaI C'yCGr_G Cuts at: 1870 1943 2808 3077 3329 1870 Size: 73 865 269 252 4545 Fragments arranged by size: 4545 865 269 252 73 BamHI G'GATC_C Cuts at: 3334 3701 3334 Size: 367 5637 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2169 2857 2926 3070 3274 Size: 325 217 627 382 688 69 144 204 Cuts at: 3274 3325 4780 618 Size: 51 1455 1842 Fragments arranged by size: 1842 1455 688 627 382 325 217 204 144 69 51 BanII G_rGCy'C Cuts at: 1391 2159 2621 2978 3001 3151 3323 1391 Size: 768 462 357 23 150 172 4072 Fragments arranged by size: 4072 768 462 357 172 150 23 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2048 2959 928 Size: 971 149 911 3973 Fragments arranged by size: 3973 971 911 149 BbvCI CC'TCA_GC Cuts at: 3109 3109 Size: 6004 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4195 4229 1903 Size: 34 2258 34 3678 Fragments arranged by size: 3678 2258 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3896 5106 3896 Size: 1210 4794 BclI T'GATC_A Cuts at: 1548 3157 1548 Size: 1609 4395 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2213 4615 136 Size: 107 122 71 631 605 541 2402 1525 Fragments arranged by size: 2402 1525 631 605 541 122 107 71 BglII A'GATC_T Cuts at: 5999 5999 Size: 6004 BlpI GC'TnA_GC Cuts at: 2153 2363 3421 2153 Size: 210 1058 4736 Fragments arranged by size: 4736 1058 210 Bme1580I G_kGCm'C Cuts at: 1790 2172 2621 2831 2860 2896 2978 3073 4065 Size: 382 449 210 29 36 82 95 992 Cuts at: 4065 5221 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 992 449 382 210 95 82 36 29 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6004 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2128 3735 4693 Size: 416 53 83 399 24 711 1607 958 Cuts at: 4693 442 Size: 1753 Fragments arranged by size: 1753 1607 958 711 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6004 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2293 2296 2429 2625 2637 4664 1239 Size: 1054 3 133 196 12 2027 2579 Fragments arranged by size: 2579 2027 1054 196 133 12 3 Bpu10I CC'TnA_GC Cuts at: 3109 3109 Size: 6004 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3122 4129 4994 5292 3122 Size: 1007 865 298 3834 Fragments arranged by size: 3834 1007 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2896 3007 4667 882 Size: 617 1397 111 1660 2219 Fragments arranged by size: 2219 1660 1397 617 111 BsaAI yAC'GTr Cuts at: 493 1204 3795 493 Size: 711 2591 2702 Fragments arranged by size: 2702 2591 711 BsaBI GATnn'nnATC Cuts at: 5998 5998 Size: 6004 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2291 2866 3811 4193 Size: 53 83 186 936 758 575 945 382 Cuts at: 4193 4828 275 Size: 635 1451 Fragments arranged by size: 1451 945 936 758 635 575 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2235 4437 4835 5109 5256 5667 2235 Size: 2202 398 274 147 411 2572 Fragments arranged by size: 2572 2202 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2417 2447 1178 Size: 30 165 30 1014 30 4735 Fragments arranged by size: 4735 1014 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2396 3081 1186 Size: 1210 685 4109 Fragments arranged by size: 4109 1210 685 BseYI C'CCAG_C Cuts at: 5207 5703 5207 Size: 496 5508 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2144 2669 1163 Size: 573 408 525 4498 Fragments arranged by size: 4498 573 525 408 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3454 4215 5243 1540 Size: 275 186 1453 761 1028 2301 Fragments arranged by size: 2301 1453 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2568 2598 2896 4065 4150 5221 2568 Size: 30 298 1169 85 1071 3351 Fragments arranged by size: 3351 1169 1071 298 85 30 BsmI GAATG_Cn' Cuts at: 3515 3608 3515 Size: 93 5911 BsmBI CGTCTCn'nnnn_ Cuts at: 2080 2080 Size: 6004 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2159 2172 2568 2598 2621 2831 2860 Size: 399 369 13 396 30 23 210 29 Cuts at: 2860 2896 2978 3001 3073 3151 3323 4065 4150 Size: 36 82 23 72 78 172 742 85 Cuts at: 4150 5221 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 396 369 210 172 85 82 78 72 36 30 29 23 23 13 BspEI T'CCGG_A Cuts at: 2235 5667 2235 Size: 3432 2572 BspHI T'CATG_A Cuts at: 3893 5623 3893 Size: 1730 4274 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2487 2592 2616 3359 844 Size: 1643 105 24 743 3489 Fragments arranged by size: 3489 1643 743 105 24 BsrBI CCG'CTC Cuts at: 1394 1823 2319 3891 1394 Size: 429 496 1572 3507 Fragments arranged by size: 3507 1572 496 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4499 4673 65 Size: 1183 3251 174 1396 Fragments arranged by size: 3251 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2148 2697 4648 4835 1700 Size: 110 338 549 1951 187 2869 Fragments arranged by size: 2869 1951 549 338 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6004 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6004 BssSI C'ACGA_G Cuts at: 4064 5076 4064 Size: 1012 4992 BstAPI GCAn_nnn'nTGC Cuts at: 794 2176 2671 2680 794 Size: 1382 495 9 4118 Fragments arranged by size: 4118 1382 495 9 BstBI TT'CG_AA Cuts at: 4808 4808 Size: 6004 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6004 BstXI CCAn_nnnn'nTGG Cuts at: 2422 2781 3024 2422 Size: 359 243 5402 Fragments arranged by size: 5402 359 243 BstYI r'GATC_y Cuts at: 1972 3334 3701 4086 4103 5555 5641 5653 5999 Size: 1362 367 385 17 1452 86 12 346 Cuts at: 5999 1972 Size: 1977 Fragments arranged by size: 1977 1452 1362 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 3288 3288 Size: 6004 Bsu36I CC'TnA_GG Cuts at: 2625 3093 2625 Size: 468 5536 BtgI C'CryG_G Cuts at: 513 1065 2004 2506 513 Size: 552 939 502 4011 Fragments arranged by size: 4011 939 552 502 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5265 Fragments arranged by size: 5265 683 56 BtsI GCAGTG_nn' Cuts at: 3147 3162 3520 4314 4334 3147 Size: 15 358 794 20 4817 Fragments arranged by size: 4817 794 358 20 15 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6004 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5969 DraI TTT'AAA Cuts at: 3308 3308 Size: 6004 DraIII CAC_nnn'GTG Cuts at: 2176 2176 Size: 6004 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5011 809 Size: 507 3695 1802 Fragments arranged by size: 3695 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3451 4340 5969 Size: 54 1425 50 275 18 1621 889 1629 Cuts at: 5969 8 Size: 43 Fragments arranged by size: 1629 1621 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3451 1537 Size: 275 1639 4090 Fragments arranged by size: 4090 1639 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3934 1353 Size: 2581 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2231 4591 4965 5111 387 Size: 1064 138 642 2360 374 146 1280 Fragments arranged by size: 2360 1280 1064 642 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2246 2293 2296 2429 2625 2637 2724 Size: 393 614 47 3 133 196 12 87 Cuts at: 2724 2904 4067 4664 5451 5719 1239 Size: 180 1163 597 787 268 1524 Fragments arranged by size: 1524 1163 787 614 597 393 268 196 180 133 87 47 12 3 EcoO109I rG'GnC_Cy Cuts at: 2242 2629 2938 2974 2975 2242 Size: 387 309 36 1 5271 Fragments arranged by size: 5271 387 309 36 1 EcoRI G'AATT_C Cuts at: 3313 3691 3313 Size: 378 5626 FspI TGC'GCA Cuts at: 4510 4510 Size: 6004 HaeII r_GCGC'y Cuts at: 1678 5151 1678 Size: 3473 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3073 3105 4645 4677 4719 Size: 32 135 32 1501 32 1540 32 42 Cuts at: 4719 4751 1373 Size: 32 2626 Fragments arranged by size: 2626 1540 1501 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2796 3348 3594 669 Size: 988 281 858 552 246 3079 Fragments arranged by size: 3079 988 858 552 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6004 HpaI GTT'AAC Cuts at: 3594 3594 Size: 6004 KpnI G_GTAC'C Cuts at: 3329 3329 Size: 6004 MfeI C'AATT_G Cuts at: 3603 3603 Size: 6004 MluI A'CGCG_T Cuts at: 5880 5880 Size: 6004 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2403 5118 5302 1305 Size: 1098 2715 184 2007 Fragments arranged by size: 2715 2007 1098 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4182 Fragments arranged by size: 4182 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3224 3962 4321 4480 518 Size: 648 2058 738 359 159 2042 Fragments arranged by size: 2058 2042 738 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2035 2362 2508 2882 3246 3420 4097 5245 Size: 429 327 146 374 364 174 677 1148 Cuts at: 5245 5490 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 429 374 364 327 245 174 146 NaeI GCC'GGC Cuts at: 2150 2699 2150 Size: 549 5455 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4513 Fragments arranged by size: 4513 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6004 NgoMIV G'CCGG_C Cuts at: 2148 2697 2148 Size: 549 5455 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6004 NotI GC'GGCC_GC Cuts at: 3451 3451 Size: 6004 NspI r_CATG'y Cuts at: 1132 1229 3362 4907 1132 Size: 97 2133 1545 2229 Fragments arranged by size: 2229 2133 1545 97 PasI CC'CwG_GG Cuts at: 1348 2614 1348 Size: 1266 4738 PciI A'CATG_T Cuts at: 4903 4903 Size: 6004 PflMI CCAn_nnn'nTGG Cuts at: 1108 2209 1108 Size: 1101 4903 PfoI T'CCnGG_A Cuts at: 2445 3008 2445 Size: 563 5441 PmeI GTTT'AAAC Cuts at: 3308 3308 Size: 6004 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4073 4105 5586 5618 4073 Size: 32 1481 32 4459 Fragments arranged by size: 4459 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2242 2242 Size: 6004 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6004 PsiI TTA'TAA Cuts at: 3574 3574 Size: 6004 PspOMI G'GGCC_C Cuts at: 2617 2974 2617 Size: 357 5647 PstI C_TGCA'G Cuts at: 830 2040 2553 2664 2881 3356 830 Size: 1210 513 111 217 475 3478 Fragments arranged by size: 3478 1210 513 475 217 111 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6004 PvuII CAG'CTG Cuts at: 2035 2882 2035 Size: 847 5157 SacII CC_GC'GG Cuts at: 2509 2509 Size: 6004 SalI G'TCGA_C Cuts at: 1936 3346 1936 Size: 1410 4594 SbfI CC_TGCA'GG Cuts at: 3356 3356 Size: 6004 ScaI AGT'ACT Cuts at: 4252 5964 4252 Size: 1712 4292 SexAI A'CCwGG_T Cuts at: 2172 2172 Size: 6004 SfcI C'TryA_G Cuts at: 826 1045 2036 2549 2660 2877 3352 4487 5168 Size: 219 991 513 111 217 475 1135 681 Cuts at: 5168 5359 826 Size: 191 1471 Fragments arranged by size: 1471 1135 991 681 513 475 219 217 191 111 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6004 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6004 SmaI CCC'GGG Cuts at: 1872 2810 3331 1872 Size: 938 521 4545 Fragments arranged by size: 4545 938 521 SmlI C'TyrA_G Cuts at: 820 1017 1943 3101 4108 5009 5271 820 Size: 197 926 1158 1007 901 262 1553 Fragments arranged by size: 1553 1158 1007 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3795 493 Size: 3302 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6004 SphI G_CATG'C Cuts at: 1132 3362 1132 Size: 2230 3774 SspI AAT'ATT Cuts at: 5 52 3928 5 Size: 47 3876 2081 Fragments arranged by size: 3876 2081 47 StuI AGG'CCT Cuts at: 1517 2460 2804 3107 1517 Size: 943 344 303 4414 Fragments arranged by size: 4414 943 344 303 StyI C'CwwG_G Cuts at: 513 1065 2004 2323 2727 3443 513 Size: 552 939 319 404 716 3074 Fragments arranged by size: 3074 939 716 552 404 319 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2129 3130 4031 4048 4201 4386 4831 Size: 1255 549 1001 901 17 153 185 445 Cuts at: 4831 325 Size: 1498 Fragments arranged by size: 1498 1255 1001 901 549 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4250 5962 96 Size: 274 80 33 51 1443 2273 1712 138 Fragments arranged by size: 2273 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5964 5996 5964 Size: 32 5972 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6004 XbaI T'CTAG_A Cuts at: 3340 3340 Size: 6004 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2024 2781 2920 2024 Size: 757 139 5108 Fragments arranged by size: 5108 757 139 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6004 XmaI C'CCGG_G Cuts at: 1870 2808 3329 1870 Size: 938 521 4545 Fragments arranged by size: 4545 938 521 XmnI GAAnn'nnTTC Cuts at: 2231 4133 2231 Size: 1902 4102 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3812 276 Size: 53 83 186 936 2278 2468 Fragments arranged by size: 2468 2278 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SanDI SapI SfiI SfoI SrfI SwaI