RFPL1 ORF clone pFN21AE1117

KOP
KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE1117

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Clone info.

Species

human

Clone Name

pFN21AE1117 Sequence (with vector) Restriction Map

Product ID

FHC06634

ORF Sequence

DNA sequence (948 bp)
Protein sequence (316 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB590097]

Gene

KIEE1117

Symbol

RFPL1

Alias : RNF78

Description

ret finger protein-like 1

Original Clone ID

oc10014

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 948 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 316 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS13857.2 7.6e-119 99.1 RFPL1 CCDS43011.1 1.9e-107 90.9 RFPL3 CCDS46694.1 2.3e-105 89.9 RFPL2 CCDS13904.1 1.1e-96 90.3 RFPL3 CCDS43009.2 1.2e-95 89.7 RFPL2 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR003879 123 140 PR01407 Butyrophylin-like IPR003879 161 185 PR01407 Butyrophylin-like IPR003879 191 204 PR01407 Butyrophylin-like IPR003879 235 259 PR01407 Butyrophylin-like IPR003879 266 284 PR01407 Butyrophylin-like HMMPfam IPR022723 65 124 PF11002 RDM domain IPR003877 177 284 PF00622 SPla/RYanodine receptor SPRY HMMSmart IPR006574 124 175 SM00589 SPRY-associated IPR018355 176 301 SM00449 SPla/RYanodine receptor subgroup ProfileScan IPR001841 40 82 PS50089 Zinc finger IPR001870 105 300 PS50188 B302/SPRY domain
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_066306 317 605968 99.1 100.0 XP_011528600 288 605968 99.0 90.8 XP_011528601 288 605968 99.0 90.8 XP_016884390 288 605968 99.0 90.8 XP_016884391 288 605968 99.0 90.8 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE1117 Download

>KIEE1117  948 bp
ATGAAAAGGTTGTCACTTGTCACAACTAACAGGCTTTCACCTCACGGAAATTTTCTTCCC
TTGTGCACTTTTCCCCTGGCAGTGGACATGGCTGCACTCTTCCAAGAAGCAAGCAGCTGT
CCCGTCTGCTCAGACTATCTAGAGAAACCAATGTCCCTGGAGTGTGGATGCGCTGTCTGC
TTCAAGTGCATCAATTCACTGCAGAAGGAGCCCCATGGGGAGGATCTACTTTGCTGTTGC
TGTTCCATGGTCTCTCAGAAGAACAAAATCAGGCCCAGTTGGCAGCTAGAGAGGCTGGCT
TCCCACATCAAGGAACTGGAGCCCAAGCTGAAGAAGATTCTGCAGATGAACCCAAGGATG
CGGAAGTTCCAAGTGGATACGACCTTGGATGCCGACACAGCCAACAACTTCCTCATTTCT
GACGACCTCAGGAGCGTCCGAAGTGGGTGCATCACACAGAATCGGCAAGACCTTGCCGAG
AGATTTGACGTGTCCATTTGCATCCTGGGCTCCCCTCGCTTTACCTGTGGCCGCCACTAC
TGGGAGGTGGACGTGGGAACAAGCACAGAATGGGACCTGGGAGTCTGCAGAGAATCTGTT
CACCGCAAAGGGAGGATCCATCTGACCACAGAGCGTGGATTCTGGACTGTGAGTTTGAGG
GATGGAAGCCGCCTCTCTGCCAGCACGGTGCCGCTGACTTTCCTCTTCGTAGACCGCAAG
TTACAGCGAGTGGGGATTTTTCTGGATATGGGCATGCAGAACGTTTCCTTTTTTGATGCT
GAAGGTGGTTCCCATGTCTATACATTCAGGAGTGTCTCTGCTGAGGAGCCACTGCACTTG
TTTTTTGCTCCTCCAAGTCCATCTAATGGTGATAAGAGTGTCTTGAGTATCTGTCCTGTG
ATAAACCCGGGCACTACTGATGCTCCAGTCCATCCTGGGGAGGCCAAA

ORF Protein

Cloned ORF protein sequence for pFN21AE1117 Download

>KIEE1117  316 aa
MKRLSLVTTNRLSPHGNFLPLCTFPLAVDMAALFQEASSCPVCSDYLEKPMSLECGCAVC
FKCINSLQKEPHGEDLLCCCCSMVSQKNKIRPSWQLERLASHIKELEPKLKKILQMNPRM
RKFQVDTTLDADTANNFLISDDLRSVRSGCITQNRQDLAERFDVSICILGSPRFTCGRHY
WEVDVGTSTEWDLGVCRESVHRKGRIHLTTERGFWTVSLRDGSRLSASTVPLTFLFVDRK
LQRVGIFLDMGMQNVSFFDAEGGSHVYTFRSVSAEEPLHLFFAPPSPSNGDKSVLSICPV
INPGTTDAPVHPGEAK

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE1117 Download

>pFN21AE1117  5653 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGAAAAGGTTGTCACTTGTCACAACTAACAGGCT
TTCACCTCACGGAAATTTTCTTCCCTTGTGCACTTTTCCCCTGGCAGTGGACATGGCTGC
ACTCTTCCAAGAAGCAAGCAGCTGTCCCGTCTGCTCAGACTATCTAGAGAAACCAATGTC
CCTGGAGTGTGGATGCGCTGTCTGCTTCAAGTGCATCAATTCACTGCAGAAGGAGCCCCA
TGGGGAGGATCTACTTTGCTGTTGCTGTTCCATGGTCTCTCAGAAGAACAAAATCAGGCC
CAGTTGGCAGCTAGAGAGGCTGGCTTCCCACATCAAGGAACTGGAGCCCAAGCTGAAGAA
GATTCTGCAGATGAACCCAAGGATGCGGAAGTTCCAAGTGGATACGACCTTGGATGCCGA
CACAGCCAACAACTTCCTCATTTCTGACGACCTCAGGAGCGTCCGAAGTGGGTGCATCAC
ACAGAATCGGCAAGACCTTGCCGAGAGATTTGACGTGTCCATTTGCATCCTGGGCTCCCC
TCGCTTTACCTGTGGCCGCCACTACTGGGAGGTGGACGTGGGAACAAGCACAGAATGGGA
CCTGGGAGTCTGCAGAGAATCTGTTCACCGCAAAGGGAGGATCCATCTGACCACAGAGCG
TGGATTCTGGACTGTGAGTTTGAGGGATGGAAGCCGCCTCTCTGCCAGCACGGTGCCGCT
GACTTTCCTCTTCGTAGACCGCAAGTTACAGCGAGTGGGGATTTTTCTGGATATGGGCAT
GCAGAACGTTTCCTTTTTTGATGCTGAAGGTGGTTCCCATGTCTATACATTCAGGAGTGT
CTCTGCTGAGGAGCCACTGCACTTGTTTTTTGCTCCTCCAAGTCCATCTAATGGTGATAA
GAGTGTCTTGAGTATCTGTCCTGTGATAAACCCGGGCACTACTGATGCTCCAGTCCATCC
TGGGGAGGCCAAAGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGAC
CTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTG
CTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATG
ATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTT
ATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAA
GTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTT
TTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTT
GCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCAC
CGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGG
GGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCG
CTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGT
ATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTT
GCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTG
GGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAA
CGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATT
GACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAG
TACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGT
GCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGA
CCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGT
TGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTA
GCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGG
CAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCC
CTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGT
ATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACG
GGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTG
ATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACT
CAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAG
CAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATA
GGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACC
CGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTG
TTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGC
TTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGG
GCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTC
TTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGA
TTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACG
GCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAA
AAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTG
TTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTT
CTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGAT
TATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTG
GATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGG
GGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGG
CGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGAT
CGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTG
TCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAG
AGGATCGAGATCT