Restriction map for
pFN21AE1117
(Linear) (Six-base) MAPSORT of: KIEE1117.seq Check: 3016 from: 1 to: 948
KIEE1117.orf
With 182 enzymes: SgfI *
July 15, 2010 18:33 ..
AccI GT'mk_AC
Cuts at: 0 710 948
Size: 710 238
AclI AA'CG_TT
Cuts at: 0 761 948
Size: 761 187
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 349 800 948
Size: 349 451 148
Fragments arranged by size:
451 349 148
AflIII A'CryG_T
Cuts at: 0 488 948
Size: 488 460
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 488 948
Size: 488 460
ApaLI G'TGCA_C
Cuts at: 0 63 948
Size: 63 885
ApoI r'AATT_y
Cuts at: 0 48 948
Size: 48 900
AvaI C'yCGr_G
Cuts at: 0 906 948
Size: 906 42
BamHI G'GATC_C
Cuts at: 0 614 948
Size: 614 334
BanI G'GyrC_C
Cuts at: 0 687 948
Size: 687 261
BanII G_rGCy'C
Cuts at: 0 212 323 511 948
Size: 212 111 188 437
Fragments arranged by size:
437 212 188 111
BbvCI CC'TCA_GC
Cuts at: 0 821 948
Size: 821 127
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 428 462 948
Size: 428 34 486
Fragments arranged by size:
486 428 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 369 948
Size: 369 579
BglI GCCn_nnn'nGGC
Cuts at: 0 279 948
Size: 279 669
Bme1580I G_kGCm'C
Cuts at: 0 67 913 948
Size: 67 846 35
Fragments arranged by size:
846 67 35
BmgBI CAC'GTC
Cuts at: 0 489 552 948
Size: 489 63 396
Fragments arranged by size:
489 396 63
BmrI ACTGGGnnnn_n'
Cuts at: 0 269 549 948
Size: 269 280 399
Fragments arranged by size:
399 280 269
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 337 908 948
Size: 178 159 571 40
Fragments arranged by size:
571 178 159 40
Bpu10I CC'TnA_GC
Cuts at: 0 821 948
Size: 821 127
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 903 948
Size: 903 45
BsaI GGTCTCn'nnnn_
Cuts at: 0 255 948
Size: 255 693
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 906 936 948
Size: 906 30 12
Fragments arranged by size:
906 30 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 838 839 948
Size: 838 1 109
Fragments arranged by size:
838 109 1
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 77 817 948
Size: 77 740 131
Fragments arranged by size:
740 131 77
BsiHKAI G_wGCw'C
Cuts at: 0 67 948
Size: 67 881
Bsp1286I G_dGCh'C
Cuts at: 0 67 212 323 511 913 948
Size: 67 145 111 188 402 35
Fragments arranged by size:
402 188 145 111 67 35
BstYI r'GATC_y
Cuts at: 0 222 614 948
Size: 222 392 334
Fragments arranged by size:
392 334 222
Bsu36I CC'TnA_GG
Cuts at: 0 427 948
Size: 427 521
BtgI C'CryG_G
Cuts at: 0 213 245 948
Size: 213 32 703
Fragments arranged by size:
703 213 32
BtsI GCAGTG_nn'
Cuts at: 0 86 196 829 948
Size: 86 110 633 119
Fragments arranged by size:
633 119 110 86
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 818 853 948
Size: 818 35 95
Fragments arranged by size:
818 95 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 580 948
Size: 580 368
EaeI y'GGCC_r
Cuts at: 0 528 948
Size: 528 420
EarI CTCTTCn'nnn_
Cuts at: 0 103 709 948
Size: 103 606 239
Fragments arranged by size:
606 239 103
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 337 349 800 908 948
Size: 178 159 12 451 108 40
Fragments arranged by size:
451 178 159 108 40 12
EcoO109I rG'GnC_Cy
Cuts at: 0 573 948
Size: 573 375
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 897 948
Size: 897 51
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 158 190 603 635 876 908 948
Size: 158 32 413 32 241 32 40
Fragments arranged by size:
413 241 158 40 32 32 32
MspA1I CmG'CkG
Cuts at: 0 116 693 948
Size: 116 577 255
Fragments arranged by size:
577 255 116
NcoI C'CATG_G
Cuts at: 0 213 245 948
Size: 213 32 703
Fragments arranged by size:
703 213 32
NspI r_CATG'y
Cuts at: 0 756 948
Size: 756 192
PflMI CCAn_nnn'nTGG
Cuts at: 0 865 948
Size: 865 83
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 581 613 948
Size: 581 32 335
Fragments arranged by size:
581 335 32
PpuMI rG'GwC_Cy
Cuts at: 0 573 948
Size: 573 375
PstI C_TGCA'G
Cuts at: 0 203 344 589 948
Size: 203 141 245 359
Fragments arranged by size:
359 245 203 141
PvuII CAG'CTG
Cuts at: 0 116 948
Size: 116 832
SfcI C'TryA_G
Cuts at: 0 199 340 585 948
Size: 199 141 245 363
Fragments arranged by size:
363 245 199 141
SmaI CCC'GGG
Cuts at: 0 908 948
Size: 908 40
SmlI C'TyrA_G
Cuts at: 0 882 948
Size: 882 66
SphI G_CATG'C
Cuts at: 0 756 948
Size: 756 192
StyI C'CwwG_G
Cuts at: 0 213 245 352 383 948
Size: 213 32 107 31 565
Fragments arranged by size:
565 213 107 32 31
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 434 948
Size: 434 514
XbaI T'CTAG_A
Cuts at: 0 138 948
Size: 138 810
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 501 948
Size: 501 447
XmaI C'CCGG_G
Cuts at: 0 906 948
Size: 906 42
XmnI GAAnn'nnTTC
Cuts at: 0 596 948
Size: 596 352
Enzymes that do cut:
AccI AclI AcuI AflIII AlfI ApaLI ApoI AvaI
BamHI BanI BanII BbvCI BcgI BciVI BglI Bme1580I
BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaXI BseRI
BsgI BsiHKAI Bsp1286I BstYI Bsu36I BtgI BtsI CspCI
DrdI EaeI EarI Eco57MI EcoO109I FalI Hin4I MspA1I
NcoI NspI PflMI PpiI PpuMI PstI PvuII SfcI
SmaI SmlI SphI StyI TaqII XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AflII AgeI AhdI AleI
AloI AlwNI ApaI AscI AseI AsiSI AvrII BaeI
BbeI BbsI BclI BfrBI BglII BlpI BmtI BplI
BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I BtgZI
ClaI DraI DraIII EagI EciI EcoICRI EcoNI EcoRI
EcoRV FseI FspI FspAI HaeII HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PfoI PmeI PmlI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SnaBI SpeI SrfI SspI StuI SwaI TatI TstI
Tth111I XhoI ZraI