Restriction map for
pFN21AE1117
(Circular) (Six-base) MAPSORT of: pFN21AE1117.seq Check: 9765 from: 1 to: 5653
pFN21AE1117 5653 bp
With 182 enzymes: SgfI *
August 16, 2010 14:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3463 278
Size: 53 83 186 936 1927 2468
Fragments arranged by size:
2468 1927 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2715 2996 1937
Size: 778 281 4594
Fragments arranged by size:
4594 778 281
Acc65I G'GTAC_C
Cuts at: 2974 2974
Size: 5653
AclI AA'CG_TT
Cuts at: 1563 2766 3780 4153 1563
Size: 1203 1014 373 3063
Fragments arranged by size:
3063 1203 1014 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2354 2805 3716 5100 5368 1632
Size: 722 451 911 1384 268 1917
Fragments arranged by size:
1917 1384 911 722 451 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5456
AflIII A'CryG_T
Cuts at: 1203 2493 4552 5529 1203
Size: 1290 2059 977 1327
Fragments arranged by size:
2059 1327 1290 977
AgeI A'CCGG_T
Cuts at: 4484 4484
Size: 5653
AhdI GACnn_n'nnGTC
Cuts at: 4382 4382
Size: 5653
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5653
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2493 1184
Size: 1309 4344
AlwNI CAG_nnn'CTG
Cuts at: 3396 4968 3396
Size: 1572 4081
ApaLI G'TGCA_C
Cuts at: 2068 3710 4866 2068
Size: 1642 1156 2855
Fragments arranged by size:
2855 1642 1156
ApoI r'AATT_y
Cuts at: 1493 2053 2962 3188 3340 5449 1493
Size: 560 909 226 152 2109 1697
Fragments arranged by size:
2109 1697 909 560 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4207 160
Size: 872 3175 1606
Fragments arranged by size:
3175 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5653
AvaI C'yCGr_G
Cuts at: 1870 1943 2911 2978 1870
Size: 73 968 67 4545
Fragments arranged by size:
4545 968 73 67
BamHI G'GATC_C
Cuts at: 2619 2983 3350 2619
Size: 364 367 4922
Fragments arranged by size:
4922 367 364
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2692 2974 4429 618
Size: 325 217 627 905 282 1455 1842
Fragments arranged by size:
1842 1455 905 627 325 282 217
BanII G_rGCy'C
Cuts at: 1391 2217 2328 2516 2972 1391
Size: 826 111 188 456 4072
Fragments arranged by size:
4072 826 456 188 111
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4682
BbvCI CC'TCA_GC
Cuts at: 2826 2826
Size: 5653
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2433 2467 3844 3878 1903
Size: 34 496 34 1377 34 3678
Fragments arranged by size:
3678 1377 496 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2374 3545 4755 2374
Size: 1171 1210 3272
Fragments arranged by size:
3272 1210 1171
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5653
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2284 4264 136
Size: 107 122 71 631 605 612 1980 1525
Fragments arranged by size:
1980 1525 631 612 605 122 107 71
BglII A'GATC_T
Cuts at: 5648 5648
Size: 5653
BlpI GC'TnA_GC
Cuts at: 3070 3070
Size: 5653
Bme1580I G_kGCm'C
Cuts at: 1790 2072 2918 3714 4870 1790
Size: 282 846 796 1156 2573
Fragments arranged by size:
2573 1156 846 796 282
BmgBI CAC'GTC
Cuts at: 1318 2494 2557 1318
Size: 1176 63 4414
Fragments arranged by size:
4414 1176 63
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2274 2554 3384
Size: 416 53 83 399 24 857 280 830
Cuts at: 3384 4342 442
Size: 958 1753
Fragments arranged by size:
1753 958 857 830 416 399 280 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5653
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2183 2342 2913 4313 1239
Size: 944 159 571 1400 2579
Fragments arranged by size:
2579 1400 944 571 159
Bpu10I CC'TnA_GC
Cuts at: 2826 2826
Size: 5653
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2908 3778 4643 4941 2908
Size: 870 865 298 3620
Fragments arranged by size:
3620 870 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2260 4316 882
Size: 617 761 2056 2219
Fragments arranged by size:
2219 2056 761 617
BsaAI yAC'GTr
Cuts at: 493 1204 3444 493
Size: 711 2240 2702
Fragments arranged by size:
2702 2240 711
BsaBI GATnn'nnATC
Cuts at: 5647 5647
Size: 5653
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3460 3842 4477 275
Size: 53 83 186 936 1927 382 635 1451
Fragments arranged by size:
1927 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4086 4484 4758 4905 5316 4086
Size: 398 274 147 411 4423
Fragments arranged by size:
4423 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2911 2941 1178
Size: 30 165 30 1508 30 3890
Fragments arranged by size:
3890 1508 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2843 2844 1186
Size: 1657 1 3995
Fragments arranged by size:
3995 1657 1
BseYI C'CCAG_C
Cuts at: 4856 5352 4856
Size: 496 5157
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2082 2822 1163
Size: 573 346 740 3994
Fragments arranged by size:
3994 740 573 346
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3103 3864 4892 1540
Size: 275 186 1102 761 1028 2301
Fragments arranged by size:
2301 1102 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2072 3714 3799 4870 2072
Size: 1642 85 1071 2855
Fragments arranged by size:
2855 1642 1071 85
BsmI GAATG_Cn'
Cuts at: 3164 3257 3164
Size: 93 5560
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2072 2217 2328 2516 2918 2972 3714
Size: 399 282 145 111 188 402 54 742
Cuts at: 3714 3799 4870 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 402 399 282 188 145
111 85 54
BspEI T'CCGG_A
Cuts at: 5316 5316
Size: 5653
BspHI T'CATG_A
Cuts at: 3542 5272 3542
Size: 1730 3923
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3008 844
Size: 2164 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3540 1394
Size: 429 1717 3507
Fragments arranged by size:
3507 1717 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4148 4322 65
Size: 1183 2900 174 1396
Fragments arranged by size:
2900 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4297 4484 1700
Size: 110 2487 187 2869
Fragments arranged by size:
2869 2487 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5653
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5653
BssSI C'ACGA_G
Cuts at: 3713 4725 3713
Size: 1012 4641
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5653
BstBI TT'CG_AA
Cuts at: 4457 4457
Size: 5653
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5653
BstYI r'GATC_y
Cuts at: 1972 2227 2619 2983 3350 3735 3752 5204 5290
Size: 255 392 364 367 385 17 1452 86
Cuts at: 5290 5302 5648 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 392 385 367 364 346 255
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2432 2432
Size: 5653
BtgI C'CryG_G
Cuts at: 513 1065 2218 2250 513
Size: 552 1153 32 3916
Fragments arranged by size:
3916 1153 552 32
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4914
Fragments arranged by size:
4914 683 56
BtsI GCAGTG_nn'
Cuts at: 2091 2201 2834 3169 3963 3983 2091
Size: 110 633 335 794 20 3761
Fragments arranged by size:
3761 794 633 335 110 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5653
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2823 2858 529
Size: 35 2259 35 3324
Fragments arranged by size:
3324 2259 35 35
DraI TTT'AAA
Cuts at: 2957 2957
Size: 5653
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2585 4660 809
Size: 507 1269 2075 1802
Fragments arranged by size:
2075 1802 1269 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2533 3100 3989
Size: 54 1425 50 275 18 703 567 889
Cuts at: 3989 5618 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 703 567 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3100 1537
Size: 275 1288 4090
Fragments arranged by size:
4090 1288 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2108 2714 3583 1353
Size: 755 606 869 3423
Fragments arranged by size:
3423 869 755 606
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4240 4614 4760 387
Size: 1064 138 2651 374 146 1280
Fragments arranged by size:
2651 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2183 2342 2354 2805 2913 3716 4313
Size: 393 551 159 12 451 108 803 597
Cuts at: 4313 5100 5368 1239
Size: 787 268 1524
Fragments arranged by size:
1524 803 787 597 551 451 393 268
159 108 12
EcoO109I rG'GnC_Cy
Cuts at: 2578 2578
Size: 5653
EcoRI G'AATT_C
Cuts at: 2962 3340 2962
Size: 378 5275
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2902 2902
Size: 5653
FspI TGC'GCA
Cuts at: 4159 4159
Size: 5653
HaeII r_GCGC'y
Cuts at: 1678 4800 1678
Size: 3122 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2163 2195 2608 2640 2881
Size: 32 135 32 591 32 413 32 241
Cuts at: 2881 2913 4294 4326 4368 4400 1373
Size: 32 1381 32 42 32 2626
Fragments arranged by size:
2626 1381 591 413 241 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2997 3243 669
Size: 988 281 1059 246 3079
Fragments arranged by size:
3079 1059 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5653
HpaI GTT'AAC
Cuts at: 3243 3243
Size: 5653
KpnI G_GTAC'C
Cuts at: 2978 2978
Size: 5653
MfeI C'AATT_G
Cuts at: 3252 3252
Size: 5653
MluI A'CGCG_T
Cuts at: 5529 5529
Size: 5653
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4767 4951 1305
Size: 3462 184 2007
Fragments arranged by size:
3462 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3831
Fragments arranged by size:
3831 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3611 3970 4129 518
Size: 648 2445 359 159 2042
Fragments arranged by size:
2445 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2121 2698 3069 3746 4894 5139 1606
Size: 515 577 371 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 577 515 371 245
NcoI C'CATG_G
Cuts at: 513 1065 2218 2250 513
Size: 552 1153 32 3916
Fragments arranged by size:
3916 1153 552 32
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5653
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5653
NotI GC'GGCC_GC
Cuts at: 3100 3100
Size: 5653
NspI r_CATG'y
Cuts at: 1132 1229 2761 3011 4556 1132
Size: 97 1532 250 1545 2229
Fragments arranged by size:
2229 1545 1532 250 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5653
PciI A'CATG_T
Cuts at: 4552 4552
Size: 5653
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2870 1108
Size: 1762 3891
PmeI GTTT'AAAC
Cuts at: 2957 2957
Size: 5653
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2586 2618 3722 3754 5235 5267 2586
Size: 32 1104 32 1481 32 2972
Fragments arranged by size:
2972 1481 1104 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2578 2578
Size: 5653
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5653
PsiI TTA'TAA
Cuts at: 3223 3223
Size: 5653
PstI C_TGCA'G
Cuts at: 830 2208 2349 2594 3005 830
Size: 1378 141 245 411 3478
Fragments arranged by size:
3478 1378 411 245 141
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5653
PvuII CAG'CTG
Cuts at: 2121 2121
Size: 5653
SalI G'TCGA_C
Cuts at: 1936 2995 1936
Size: 1059 4594
SbfI CC_TGCA'GG
Cuts at: 3005 3005
Size: 5653
ScaI AGT'ACT
Cuts at: 3901 5613 3901
Size: 1712 3941
SfcI C'TryA_G
Cuts at: 826 1045 2204 2345 2590 3001 4136 4817 5008
Size: 219 1159 141 245 411 1135 681 191
Cuts at: 5008 826
Size: 1471
Fragments arranged by size:
1471 1159 1135 681 411 245 219 191
141
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5653
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5653
SmaI CCC'GGG
Cuts at: 1872 2913 2980 1872
Size: 1041 67 4545
Fragments arranged by size:
4545 1041 67
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2887 3757 4658 4920 820
Size: 197 926 944 870 901 262 1553
Fragments arranged by size:
1553 944 926 901 870 262 197
SnaBI TAC'GTA
Cuts at: 493 3444 493
Size: 2951 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5653
SphI G_CATG'C
Cuts at: 1132 2761 3011 1132
Size: 1629 250 3774
Fragments arranged by size:
3774 1629 250
SspI AAT'ATT
Cuts at: 5 52 3577 5
Size: 47 3525 2081
Fragments arranged by size:
3525 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5653
StyI C'CwwG_G
Cuts at: 513 1065 2218 2250 2357 2388 3092 513
Size: 552 1153 32 107 31 704 3074
Fragments arranged by size:
3074 1153 704 552 107 32 31
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2439 3680 3697 3850 4035 4480 325
Size: 1255 859 1241 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1241 859 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3899 5611 96
Size: 274 80 33 51 1443 1922 1712 138
Fragments arranged by size:
1922 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5613 5645 5613
Size: 32 5621
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5653
XbaI T'CTAG_A
Cuts at: 2143 2989 2143
Size: 846 4807
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2506 2506
Size: 5653
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5653
XmaI C'CCGG_G
Cuts at: 1870 2911 2978 1870
Size: 1041 67 4545
Fragments arranged by size:
4545 1041 67
XmnI GAAnn'nnTTC
Cuts at: 2601 3782 2601
Size: 1181 4472
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3461 276
Size: 53 83 186 936 1927 2468
Fragments arranged by size:
2468 1927 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI
EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI