################################## # NO CUT ENZYMES ( 144 enzymes) # ################################## AatII Acc65I AccII AccIII AcyI AfaI AflII AgeI AhdI AlwNI Aor51HI ApaI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BalI BbeI BbrPI BbsI BclI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I CelII Cfr10I ClaI CpoI DraI DraIII EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MseI MslI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI PvuI RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI TaqI Tru9I Tth111I TthHB8I XhoI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AccI 709 GTAGAC GT^[AC][GT]AC AclI 760 AACGTT AA^CGTT AcsI 48 AAATTT [GA]^AATT[CT] AflIII 488 ACGTGT A^C[GA][CT]GT Alw44I 63 GTGCAC G^TGCAC AocI 426 CCTCAGG CC^T[ACGT]AGG ApaLI 63 GTGCAC G^TGCAC ApoI 48 AAATTT [GA]^AATT[CT] Asp700I 592 GAATCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 63 GTGCAC G[AT]GC[AT]^C AvaI 906 CCCGGG C^[CT]CG[GA]G AvaII 573 GGACC G^G[AT]CC BamHI 614 GGATCC G^GATCC BanI 687 GGTGCC G^G[CT][GA]CC BbvCI 820 GCTGAGG CCTCAGC(-5/-2) BcgI 439 CGAAGTGGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 375 GGATAC GTATCC(6/5) BglI 273 GCCCAGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaI 249 GGTCTC GGTCTC(1/5) BsiHKAI 63 GTGCAC G[AT]GC[AT]^C BsoBI 906 CCCGGG C^[CT]CG[GA]G Bsu36I 426 CCTCAGG CC^T[ACGT]AGG CfoI 170 GCGC GCG^C DraII 572 GGGACCT [GA]G^G[ACGT]CC[CT] DrdI 574 GACCTGGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 528 TGGCCG [CT]^GGCC[GA] Eco81I 426 CCTCAGG CC^T[ACGT]AGG EcoO109I 572 GGGACCT [GA]G^G[ACGT]CC[CT] HapII 907 CCGG C^CGG HgaI 434 GCGTC GACGC(5/10) HhaI 170 GCGC GCG^C HinP1I 170 GCGC G^CGC HpaII 907 CCGG C^CGG MspI 907 CCGG C^CGG NspI 752 GCATGC [GA]CATG^[CT] PflMI 859 CCATCTAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 581 GAGTC GAGTC(4/5) PpuMI 572 GGGACCT [GA]G^G[AT]CC[CT] Psp1406I 760 AACGTT AA^CGTT PvuII 114 CAGCTG CAG^CTG SmaI 906 CCCGGG CCC^GGG SmlI 882 CTTGAG C^T[CT][GA]AG SphI 752 GCATGC GCATG^C Van91I 859 CCATCTAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 138 TCTAGA T^CTAGA XcmI 494 CCATTTGCATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 906 CCCGGG C^CCGGG XmaI 906 CCCGGG C^CCGGG XmnI 592 GAATCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### BcnI 906 CCCGG CC^[GC]GG 907 CCGGG BfaI 139 CTAG C^TAG 286 CTAG BseRI 848 CTCCTC GAGGAG(10/8) 823 GAGGAG BsgI 92 CTGCAC GTGCAG(16/14) 832 CTGCAC BsmAI 250 GTCTC GTCTC(1/5) 814 GTCTC BstYI 222 GGATCT [GA]^GATC[CT] 614 GGATCC Cfr13I 272 GGCCC G^G[ACGT]CC 573 GGACC DpnII 223 GATC ^GATC 615 GATC DsaI 213 CCATGG C^C[GA][CT]GG 245 CCATGG EarI 97 CTCTTC CTCTTC(1/4) 703 CTCTTC Ksp632I 97 CTCTTC CTCTTC(1/4) 703 CTCTTC MaeI 139 CTAG C^TAG 286 CTAG MboI 223 GATC ^GATC 615 GATC MflI 222 GGATCT [GA]^GATC[CT] 614 GGATCC MspA1I 114 CAGCTG C[AC]G^C[GT]G 691 CCGCTG NciI 906 CCCGG CC^[GC]GG 907 CCGGG NcoI 213 CCATGG C^CATGG 245 CCATGG NdeII 223 GATC ^GATC 615 GATC Sau3AI 223 GATC ^GATC 615 GATC Sau96I 272 GGCCC G^G[ACGT]CC 573 GGACC Tsp45I 12 GTCAC ^GT[GC]AC 19 GTCAC Tsp509I 49 AATT ^AATT 193 AATT XhoII 222 GGATCT [GA]^GATC[CT] 614 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 42 enzymes) # ######################################### AciI AluI AlwI BanII BbvI BmyI BpmI BsaJI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I DdeI EcoRII EcoT14I Fnu4HI FokI HaeIII HinfI HphI ItaI MaeII MaeIII MboII MnlI MvaI MwoI NlaIII NlaIV PstI ScrFI SfaNI SfcI StyI TfiI TseI TspRI