POU4F1 ORF clone pFN21AB9724

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AB9724

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Clone info.

Species

human

Clone Name

pFN21AB9724 Sequence (with vector) Restriction Map

Product ID

FHC16557

ORF Sequence

DNA sequence (1254 bp)
Protein sequence (418 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIBB9724

Symbol

POU4F1

Alias : ATITHS, BRN3A, Oct-T1, RDC-1, brn-3A

Description

POU class 4 homeobox 1

Original Clone ID

cp00307

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1254 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 418 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS31996.1 2.1e-68 99.8 POU4F1 CCDS34074.1 5.9e-22 58.7 POU4F2 CCDS4281.1 3.6e-21 60.5 POU4F3 CCDS30679.1 1.3e-11 44.2 POU3F1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR013847 278 295 PR00028 POU IPR013847 304 317 PR00028 POU IPR013847 320 335 PR00028 POU IPR013847 353 373 PR00028 POU IPR013847 388 403 PR00028 POU HMMPfam IPR000327 261 336 PF00157 POU-specific IPR001356 355 411 PF00046 Homeobox HMMSmart IPR000327 259 336 SM00352 POU-specific IPR001356 354 416 SM00389 Homeobox ProfileScan IPR000327 259 336 PS51179 POU-specific IPR001356 352 412 PS50071 Homeobox
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_006228 419 601632 99.8 100.0 NP_004566 409 113725 58.7 92.8 NP_002691 338 602459,602460 60.5 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AB9724 Download

>KIBB9724  1254 bp
ATGATGTCCATGAACAGCAAGCAGCCTCACTTTGCCATGCATCCCACCCTCCCTGAGCAC
AAGTACCCGTCGCTGCACTCCAGCTCCGAGGCCATCCGGCGGGCCTGCCTGCCCACGCCG
CCGCTGCAGAGCAACCTCTTCGCCAGCCTGGACGAGACGCTGCTGGCGCGGGCCGAGGCG
CTGGCGGCCGTGGACATCGCCGTGTCCCAGGGCAAGAGCCATCCTTTCAAGCCGGACGCC
ACGTACCACACGATGAACAGCGTGCCGTGCACGTCCACTTCCACGGTGCCTCTGGCGCAC
CACCACCACCACCACCACCACCAGGCGCTCGAACCCGGCGATCTGCTGGACCACATCTCC
TCGCCGTCGCTCGCGCTCATGGCCGGCGCGGGCGGCGCGGGCGCGGCGGCCGGCGGCGGC
GGCGCCCACGACGGCCCGGGGGGCGGTGGCGGCCCGGGCGGCGGCGGCGGCCCGGGCGGC
GGCCCCGGGGGAGGCGGCGGTGGCGGCCCGGGGGGCGGCGGCGGCGGCCCGGGCGGCGGG
CTCCTGGGCGGCTCCGCGCACCCTCACCCGCATATGCACAGCCTGGGCCACCTGTCGCAC
CCCGCGGCGGCGGCCGCCATGAACATGCCGTCCGGGCTGCCGCACCCCGGGCTGGTGGCG
GCGGCGGCGCACCACGGCGCGGCAGCGGCAGCGGCGGCGGCGGCGGCCGGGCAGGTGGCA
GCGGCATCGGCGGCGGCGGCCGTGGTGGGCGCAGCGGGCCTGGCGTCCATCTGCGACTCG
GACACGGACCCGCGCGAGCTCGAGGCGTTCGCGGAGCGCTTCAAGCAGCGGCGCATCAAG
CTGGGCGTGACGCAGGCCGACGTGGGCTCGGCGCTGGCCAACCTCAAGATCCCGGGCGTG
GGCTCACTCAGCCAGAGCACCATCTGCAGGTTCGAGTCGCTCACGCTCTCGCACAACAAC
ATGATCGCGCTCAAGCCCATCCTGCAGGCGTGGCTCGAGGAGGCCGAGGGCGCCCAGCGC
GAGAAAATGAACAAGCCTGAGCTCTTCAACGGCGGCGAGAAGAAGCGCAAGCGGACTTCC
ATCGCCGCGCCCGAGAAGCGCTCCCTCGAGGCCTACTTCGCCGTGCAGCCCCGGCCCTCG
TCCGAGAAGATCGCCGCCATCGCCGAGAAACTGGACCTCAAAAAGAACGTGGTGCGGGTG
TGGTTTTGCAACCAGAGACAGAAGCAGAAGCGGATGAAATTCTCTGCCACTTAC

ORF Protein

Cloned ORF protein sequence for pFN21AB9724 Download

>KIBB9724  418 aa
MMSMNSKQPHFAMHPTLPEHKYPSLHSSSEAIRRACLPTPPLQSNLFASLDETLLARAEA
LAAVDIAVSQGKSHPFKPDATYHTMNSVPCTSTSTVPLAHHHHHHHHQALEPGDLLDHIS
SPSLALMAGAGGAGAAAGGGGAHDGPGGGGGPGGGGGPGGGPGGGGGGGPGGGGGGPGGG
LLGGSAHPHPHMHSLGHLSHPAAAAAMNMPSGLPHPGLVAAAAHHGAAAAAAAAAAGQVA
AASAAAAVVGAAGLASICDSDTDPRELEAFAERFKQRRIKLGVTQADVGSALANLKIPGV
GSLSQSTICRFESLTLSHNNMIALKPILQAWLEEAEGAQREKMNKPELFNGGEKKRKRTS
IAAPEKRSLEAYFAVQPRPSSEKIAAIAEKLDLKKNVVRVWFCNQRQKQKRMKFSATY

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AB9724 Download

>pFN21AB9724  5959 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGATGTCCATGAACAGCAAGCAGCCTCACTTTGC
CATGCATCCCACCCTCCCTGAGCACAAGTACCCGTCGCTGCACTCCAGCTCCGAGGCCAT
CCGGCGGGCCTGCCTGCCCACGCCGCCGCTGCAGAGCAACCTCTTCGCCAGCCTGGACGA
GACGCTGCTGGCGCGGGCCGAGGCGCTGGCGGCCGTGGACATCGCCGTGTCCCAGGGCAA
GAGCCATCCTTTCAAGCCGGACGCCACGTACCACACGATGAACAGCGTGCCGTGCACGTC
CACTTCCACGGTGCCTCTGGCGCACCACCACCACCACCACCACCACCAGGCGCTCGAACC
CGGCGATCTGCTGGACCACATCTCCTCGCCGTCGCTCGCGCTCATGGCCGGCGCGGGCGG
CGCGGGCGCGGCGGCCGGCGGCGGCGGCGCCCACGACGGCCCGGGGGGCGGTGGCGGCCC
GGGCGGCGGCGGCGGCCCGGGCGGCGGCCCCGGGGGAGGCGGCGGTGGCGGCCCGGGGGG
CGGCGGCGGCGGCCCGGGCGGCGGGCTCCTGGGCGGCTCCGCGCACCCTCACCCGCATAT
GCACAGCCTGGGCCACCTGTCGCACCCCGCGGCGGCGGCCGCCATGAACATGCCGTCCGG
GCTGCCGCACCCCGGGCTGGTGGCGGCGGCGGCGCACCACGGCGCGGCAGCGGCAGCGGC
GGCGGCGGCGGCCGGGCAGGTGGCAGCGGCATCGGCGGCGGCGGCCGTGGTGGGCGCAGC
GGGCCTGGCGTCCATCTGCGACTCGGACACGGACCCGCGCGAGCTCGAGGCGTTCGCGGA
GCGCTTCAAGCAGCGGCGCATCAAGCTGGGCGTGACGCAGGCCGACGTGGGCTCGGCGCT
GGCCAACCTCAAGATCCCGGGCGTGGGCTCACTCAGCCAGAGCACCATCTGCAGGTTCGA
GTCGCTCACGCTCTCGCACAACAACATGATCGCGCTCAAGCCCATCCTGCAGGCGTGGCT
CGAGGAGGCCGAGGGCGCCCAGCGCGAGAAAATGAACAAGCCTGAGCTCTTCAACGGCGG
CGAGAAGAAGCGCAAGCGGACTTCCATCGCCGCGCCCGAGAAGCGCTCCCTCGAGGCCTA
CTTCGCCGTGCAGCCCCGGCCCTCGTCCGAGAAGATCGCCGCCATCGCCGAGAAACTGGA
CCTCAAAAAGAACGTGGTGCGGGTGTGGTTTTGCAACCAGAGACAGAAGCAGAAGCGGAT
GAAATTCTCTGCCACTTACGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGA
GTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGT
TGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCA
GACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAA
TGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAAT
AAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGG
GAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATC
CTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGT
CAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACT
TTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATG
TATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCT
GTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCA
CGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCC
GAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCC
CGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTG
GTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTA
TGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATC
GGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTT
GATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATG
CCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCT
TCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGC
TCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCT
CGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTAC
ACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCC
TCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAG
CTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACA
TGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTT
TCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGC
GAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCT
CTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCG
TGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCA
AGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACT
ATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTA
ACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTA
ACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCT
TCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTT
TTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGA
TCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCA
TGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGC
ACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATT
AAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTT
ATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGC
CGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCAC
TTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCG
GCGTAGAGGATCGAGATCT