################################## # NO CUT ENZYMES ( 126 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AflIII AgeI AhdI AlwNI AocI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbrPI BbsI BbvCI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaI BsaWI BseAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DraII DraIII DrdI Eam1105I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I FbaI FseI FspI HincII HindII HindIII HpaI KpnI MaeI MamI MfeI MluI MroI MseI MunI NcoI NheI NruI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SalI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI SphI SspBI SspI StyI SwaI TfiI Tru9I TspRI Tth111I Van91I XbaI XmnI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### AcsI 1237 AAATTC [GA]^AATT[CT] Alw44I 267 GTGCAC G^TGCAC AlwI 888 GATCC GGATC(4/5) ApaLI 267 GTGCAC G^TGCAC ApoI 1237 AAATTC [GA]^AATT[CT] BalI 875 TGGCCA TGG^CCA BpmI 78 CTCCAG CTGGAG(16/14) BsaAI 240 CACGTA [CT]AC^GT[GA] BsmBI 154 GAGACG CGTCTC(1/5) BsmFI 204 GTCCC GGGAC(10/14) BsrI 1170 ACTGG ACTGG(1/-1) BstAPI 917 GCACCATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 887 AGATCC [GA]^GATC[CT] EcoT22I 37 ATGCAT ATGCA^T HphI 564 TCACC GGTGA(8/7) KspI 602 CCGCGG CCGC^GG MaeIII 847 GTGAC ^GT[ACGT]AC MflI 887 AGATCC [GA]^GATC[CT] MluNI 875 TGGCCA TGG^CCA MscI 875 TGGCCA TGG^CCA NdeI 571 CATATG CA^TATG NotI 610 GCGGCCGC GC^GGCCGC NsiI 37 ATGCAT ATGCA^T NspI 623 ACATGC [GA]CATG^[CT] SacII 602 CCGCGG CCGC^GG SapI 1041 GCTCTTC GCTCTTC(1/4) SbfI 981 CCTGCAGG CCTGCA^GG Sse8387I 981 CCTGCAGG CCTGCA^GG StuI 1109 AGGCCT AGG^CCT Tsp45I 847 GTGAC ^GT[GC]AC Tsp509I 1238 AATT ^AATT XcmI 281 CCACGGTGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 887 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AfaI 63 GTAC GT^AC 243 GTAC Aor51HI 815 AGCGCT AGC^GCT 1097 AGCGCT BbeI 421 GGCGCC GGCGC^C 1009 GGCGCC BcgI 131 GCAACCTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 718 GCAGCGGCATCG BseRI 357 CTCCTC GAGGAG(10/8) 997 GAGGAG BsgI 73 CTGCAC GTGCAG(16/14) 1123 GTGCAG BsmAI 154 GAGAC GTCTC(1/5) 1215 GAGAC BspMI 711 GCAGGT ACCTGC(4/8) 926 GCAGGT BsrFI 382 GCCGGC [GA]^CCGG[CT] 409 GCCGGC Cfr10I 382 GCCGGC [GA]^CCGG[CT] 409 GCCGGC EarI 136 CTCTTC CTCTTC(1/4) 1042 CTCTTC Eco47III 815 AGCGCT AGC^GCT 1097 AGCGCT HinfI 775 GACTC G^A[ACGT]TC 934 GAGTC KasI 421 GGCGCC G^GCGCC 1009 GGCGCC Ksp632I 136 CTCTTC CTCTTC(1/4) 1042 CTCTTC MslI 195 CATCGCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 618 CATGAACATG NaeI 382 GCCGGC GCC^GGC 409 GCCGGC NarI 421 GGCGCC GG^CGCC 1009 GGCGCC NgoMIV 382 GCCGGC G^CCGGC 409 GCCGGC PleI 775 GACTC GAGTC(4/5) 934 GAGTC RsaI 63 GTAC GT^AC 243 GTAC SacI 796 GAGCTC GAGCT^C 1039 GAGCTC SfoI 421 GGCGCC GGC^GCC 1009 GGCGCC ######################################### # 3 OR MORE CUT ENZYMES ( 76 enzymes) # ######################################### AccII AciI AcyI AluI AspHI AvaI AvaII BanI BanII BbvI BcnI BglI BmyI BsaHI BsaJI BsiEI BsiHKAI BsiYI BslI BsoBI Bsp1286I BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DsaI EaeI EagI EclXI Eco52I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HpaII ItaI MaeII MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PaeR7I PstI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI TaqI TseI TthHB8I XhoI XmaCI XmaI