(Circular) (Six-base) MAPSORT of: pFN21AB9724.seq Check: 9714 from: 1 to: 5959 pFN21AB9724 5959 bp With 182 enzymes: SgfI * December 8, 2014 13:47 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2707 2707 Size: 5959 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3769 278 Size: 53 83 186 936 2233 2468 Fragments arranged by size: 2468 2233 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3302 1937 Size: 1365 4594 Acc65I G'GTAC_C Cuts at: 3280 3280 Size: 5959 AclI AA'CG_TT Cuts at: 1563 4086 4459 1563 Size: 2523 373 3063 Fragments arranged by size: 3063 2523 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4022 5406 5674 1632 Size: 2390 1384 268 1917 Fragments arranged by size: 2390 1917 1384 268 AfeI AGC'GCT Cuts at: 2822 3104 2822 Size: 282 5677 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5762 AflIII A'CryG_T Cuts at: 1203 4858 5835 1203 Size: 3655 977 1327 Fragments arranged by size: 3655 1327 977 AgeI A'CCGG_T Cuts at: 4790 4790 Size: 5959 AhdI GACnn_n'nnGTC Cuts at: 4688 4688 Size: 5959 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5959 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5959 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2618 2650 2618 Size: 32 5927 AlwNI CAG_nnn'CTG Cuts at: 3702 5274 3702 Size: 1572 4387 ApaLI G'TGCA_C Cuts at: 2272 4016 5172 2272 Size: 1744 1156 3059 Fragments arranged by size: 3059 1744 1156 ApoI r'AATT_y Cuts at: 1493 3242 3268 3494 3646 5755 1493 Size: 1749 26 226 152 2109 1697 Fragments arranged by size: 2109 1749 1697 226 152 26 AseI AT'TA_AT Cuts at: 160 1032 4513 160 Size: 872 3481 1606 Fragments arranged by size: 3481 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5959 AvaI C'yCGr_G Cuts at: 1870 1943 2440 2458 2476 2489 2512 2533 2651 Size: 73 497 18 18 13 23 21 118 Cuts at: 2651 2804 2896 2999 3095 3110 3284 1870 Size: 153 92 103 96 15 174 4545 Fragments arranged by size: 4545 497 174 153 118 103 96 92 73 23 21 18 18 15 13 BamHI G'GATC_C Cuts at: 3289 3656 3289 Size: 367 5592 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2290 2426 3014 3280 4735 Size: 325 217 627 503 136 588 266 1455 Cuts at: 4735 618 Size: 1842 Fragments arranged by size: 1842 1455 627 588 503 325 266 217 136 BanII G_rGCy'C Cuts at: 1391 2547 2805 2873 2909 3048 3278 1391 Size: 1156 258 68 36 139 230 4072 Fragments arranged by size: 4072 1156 258 230 139 68 36 BbeI G_GCGC'C Cuts at: 2430 3018 2430 Size: 588 5371 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4988 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2125 2159 2712 2746 4150 4184 1903 Size: 34 188 34 553 34 1404 34 3678 Fragments arranged by size: 3678 1404 553 188 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3851 5061 3851 Size: 1210 4749 BclI T'GATC_A Cuts at: 1548 1548 Size: 5959 BfrBI ATG'CAT Cuts at: 2044 2044 Size: 5959 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2445 2463 2481 Size: 107 122 71 631 605 773 18 18 Cuts at: 2481 2517 2538 2638 4570 136 Size: 36 21 100 1932 1525 Fragments arranged by size: 1932 1525 773 631 605 122 107 100 71 36 21 18 18 BglII A'GATC_T Cuts at: 5954 5954 Size: 5959 BlpI GC'TnA_GC Cuts at: 3376 3376 Size: 5959 Bme1580I G_kGCm'C Cuts at: 1790 2276 4020 5176 1790 Size: 486 1744 1156 2573 Fragments arranged by size: 2573 1744 1156 486 BmgBI CAC'GTC Cuts at: 1318 2277 2866 1318 Size: 959 589 4411 Fragments arranged by size: 4411 959 589 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3690 4648 442 Size: 416 53 83 399 24 2273 958 1753 Fragments arranged by size: 2273 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5959 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3121 3121 Size: 5959 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2068 4619 1239 Size: 829 2551 2579 Fragments arranged by size: 2579 2551 829 Bpu10I CC'TnA_GC Cuts at: 2058 3042 2058 Size: 984 4975 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2873 2960 4084 4949 5247 2873 Size: 87 1124 865 298 3585 Fragments arranged by size: 3585 1124 865 298 87 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4622 882 Size: 617 3123 2219 Fragments arranged by size: 3123 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2247 3750 493 Size: 711 1043 1503 2702 Fragments arranged by size: 2702 1503 1043 711 BsaBI GATnn'nnATC Cuts at: 5953 5953 Size: 5959 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2241 2427 2768 3015 Size: 53 83 186 936 708 186 341 247 Cuts at: 3015 3766 4148 4783 275 Size: 751 382 635 1451 Fragments arranged by size: 1451 936 751 708 635 382 341 247 186 186 83 53 BsaWI w'CCGG_w Cuts at: 4392 4790 5064 5211 5622 4392 Size: 398 274 147 411 4729 Fragments arranged by size: 4729 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2072 2102 2345 2375 1178 Size: 30 165 30 669 30 243 30 4762 Fragments arranged by size: 4762 669 243 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2353 3017 1186 Size: 1167 664 4128 Fragments arranged by size: 4128 1167 664 BseYI C'CCAG_C Cuts at: 2845 3018 5162 5658 2845 Size: 173 2144 496 3146 Fragments arranged by size: 3146 2144 496 173 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2063 3149 1163 Size: 573 327 1086 3973 Fragments arranged by size: 3973 1086 573 327 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2193 2415 2619 2712 2745 3409 Size: 275 186 192 222 204 93 33 664 Cuts at: 3409 4170 5198 1540 Size: 761 1028 2301 Fragments arranged by size: 2301 1028 761 664 275 222 204 192 186 93 33 BsiHKAI G_wGCw'C Cuts at: 2064 2276 2805 2924 3048 4020 4105 5176 2064 Size: 212 529 119 124 972 85 1071 2847 Fragments arranged by size: 2847 1071 972 529 212 124 119 85 BsmI GAATG_Cn' Cuts at: 3470 3563 3470 Size: 93 5866 BsmBI CGTCTCn'nnnn_ Cuts at: 2153 2153 Size: 5959 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2064 2276 2547 2805 2873 2909 2924 Size: 399 274 212 271 258 68 36 15 Cuts at: 2924 3048 3278 4020 4105 5176 1391 Size: 124 230 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 274 271 258 230 212 124 85 68 36 15 BspEI T'CCGG_A Cuts at: 5622 5622 Size: 5959 BspHI T'CATG_A Cuts at: 3848 5578 3848 Size: 1730 4229 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2707 2922 3314 844 Size: 1863 215 392 3489 Fragments arranged by size: 3489 1863 392 215 BsrBI CCG'CTC Cuts at: 1394 1823 3846 1394 Size: 429 2023 3507 Fragments arranged by size: 3507 2023 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4454 4628 65 Size: 1183 3206 174 1396 Fragments arranged by size: 3206 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2387 2414 4603 4790 1700 Size: 110 577 27 2189 187 2869 Fragments arranged by size: 2869 2189 577 187 110 27 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5959 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5959 BssSI C'ACGA_G Cuts at: 4019 5031 4019 Size: 1012 4947 BstAPI GCAn_nnn'nTGC Cuts at: 794 2928 794 Size: 2134 3825 BstBI TT'CG_AA Cuts at: 4763 4763 Size: 5959 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5959 BstYI r'GATC_y Cuts at: 1972 2892 3289 3656 4041 4058 5510 5596 5608 Size: 920 397 367 385 17 1452 86 12 Cuts at: 5608 5954 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 920 397 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2193 2286 2607 2677 2745 513 Size: 552 1128 93 321 70 68 3727 Fragments arranged by size: 3727 1128 552 321 93 70 68 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2185 3070 3148 487 Size: 683 56 959 885 78 3298 Fragments arranged by size: 3298 959 885 683 78 56 BtsI GCAGTG_nn' Cuts at: 3475 4269 4289 3475 Size: 794 20 5145 Fragments arranged by size: 5145 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5959 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5924 DraI TTT'AAA Cuts at: 3263 3263 Size: 5959 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4966 809 Size: 507 3650 1802 Fragments arranged by size: 3650 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2190 2385 2412 Size: 54 1425 50 275 18 360 195 27 Cuts at: 2412 2616 2709 2742 2880 3406 4295 5924 8 Size: 204 93 33 138 526 889 1629 43 Fragments arranged by size: 1629 1425 889 526 360 275 204 195 138 93 54 50 43 33 27 18 EagI C'GGCC_G Cuts at: 1537 1812 2190 2412 2616 2709 2742 3406 1537 Size: 275 378 222 204 93 33 664 4090 Fragments arranged by size: 4090 664 378 275 222 204 93 33 EarI CTCTTCn'nnn_ Cuts at: 1353 2147 3053 3889 1353 Size: 794 906 836 3423 Fragments arranged by size: 3423 906 836 794 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4546 4920 5066 387 Size: 1064 138 2957 374 146 1280 Fragments arranged by size: 2957 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2803 3046 2803 Size: 243 5716 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2068 4022 4619 5406 5674 1239 Size: 393 436 1954 597 787 268 1524 Fragments arranged by size: 1954 1524 787 597 436 393 268 EcoRI G'AATT_C Cuts at: 3268 3646 3268 Size: 378 5581 FspI TGC'GCA Cuts at: 4465 4465 Size: 5959 HaeII r_GCGC'y Cuts at: 1678 2186 2333 2430 2824 2879 3018 3106 5106 Size: 508 147 97 394 55 139 88 2000 Cuts at: 5106 1678 Size: 2531 Fragments arranged by size: 2531 2000 508 394 147 139 97 88 55 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2345 2377 2855 2887 4600 Size: 32 135 32 773 32 478 32 1713 Cuts at: 4600 4632 4674 4706 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1713 773 478 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3303 3549 669 Size: 988 281 1365 246 3079 Fragments arranged by size: 3079 1365 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5959 HpaI GTT'AAC Cuts at: 3549 3549 Size: 5959 KasI G'GCGC_C Cuts at: 2426 3014 2426 Size: 588 5371 KpnI G_GTAC'C Cuts at: 3284 3284 Size: 5959 MfeI C'AATT_G Cuts at: 3558 3558 Size: 5959 MluI A'CGCG_T Cuts at: 5835 5835 Size: 5959 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5073 5257 1305 Size: 3768 184 2007 Fragments arranged by size: 3768 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2882 10 Size: 54 1425 343 1050 3087 Fragments arranged by size: 3087 1425 1050 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2204 2627 3917 4276 4435 518 Size: 648 1038 423 1290 359 159 2042 Fragments arranged by size: 2042 1290 1038 648 423 359 159 MspA1I CmG'CkG Cuts at: 1606 2128 2609 2690 2696 2726 2759 2833 3375 Size: 522 481 81 6 30 33 74 542 Cuts at: 3375 4052 5200 5445 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 542 522 481 245 81 74 33 30 6 NaeI GCC'GGC Cuts at: 2389 2416 2389 Size: 27 5932 NarI GG'CG_CC Cuts at: 2427 3015 2427 Size: 588 5371 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5407 NdeI CA'TA_TG Cuts at: 387 2577 387 Size: 2190 3769 NgoMIV G'CCGG_C Cuts at: 2387 2414 2387 Size: 27 5932 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5959 NotI GC'GGCC_GC Cuts at: 2616 3406 2616 Size: 790 5169 NsiI A_TGCA'T Cuts at: 2046 2046 Size: 5959 NspI r_CATG'y Cuts at: 1132 1229 2632 3317 4862 1132 Size: 97 1403 685 1545 2229 Fragments arranged by size: 2229 1545 1403 685 97 PasI CC'CwG_GG Cuts at: 1348 2212 1348 Size: 864 5095 PciI A'CATG_T Cuts at: 4858 4858 Size: 5959 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5959 PmeI GTTT'AAAC Cuts at: 3263 3263 Size: 5959 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4028 4060 5541 5573 4028 Size: 32 1481 32 4414 Fragments arranged by size: 4414 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5959 PsiI TTA'TAA Cuts at: 3529 3529 Size: 5959 PspXI vC'TCGA_Gb Cuts at: 2804 2999 3110 2804 Size: 195 111 5653 Fragments arranged by size: 5653 195 111 PstI C_TGCA'G Cuts at: 830 2133 2933 2991 3311 830 Size: 1303 800 58 320 3478 Fragments arranged by size: 3478 1303 800 320 58 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5959 SacI G_AGCT'C Cuts at: 2805 3048 2805 Size: 243 5716 SacII CC_GC'GG Cuts at: 2610 2610 Size: 5959 SalI G'TCGA_C Cuts at: 1936 3301 1936 Size: 1365 4594 SapI GCTCTTCn'nnn_ Cuts at: 3053 3053 Size: 5959 SbfI CC_TGCA'GG Cuts at: 2991 3311 2991 Size: 320 5639 ScaI AGT'ACT Cuts at: 4207 5919 4207 Size: 1712 4247 SfcI C'TryA_G Cuts at: 826 1045 2129 2929 2987 3307 4442 5123 5314 Size: 219 1084 800 58 320 1135 681 191 Cuts at: 5314 826 Size: 1471 Fragments arranged by size: 1471 1135 1084 800 681 320 219 191 58 SfoI GGC'GCC Cuts at: 2428 3016 2428 Size: 588 5371 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5959 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5959 SmaI CCC'GGG Cuts at: 1872 2442 2460 2478 2491 2514 2535 2653 2898 Size: 570 18 18 13 23 21 118 245 Cuts at: 2898 3286 1872 Size: 388 4545 Fragments arranged by size: 4545 570 388 245 118 23 21 18 18 13 SmlI C'TyrA_G Cuts at: 820 1017 1943 2804 2888 2975 2999 3110 4063 Size: 197 926 861 84 87 24 111 953 Cuts at: 4063 4964 5226 820 Size: 901 262 1553 Fragments arranged by size: 1553 953 926 901 861 262 197 111 87 84 24 SnaBI TAC'GTA Cuts at: 493 3750 493 Size: 3257 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5959 SphI G_CATG'C Cuts at: 1132 3317 1132 Size: 2185 3774 SrfI GCCC'GGGC Cuts at: 2460 2478 2535 2460 Size: 18 57 5884 Fragments arranged by size: 5884 57 18 SspI AAT'ATT Cuts at: 5 52 3883 5 Size: 47 3831 2081 Fragments arranged by size: 3831 2081 47 StuI AGG'CCT Cuts at: 1517 3116 1517 Size: 1599 4360 StyI C'CwwG_G Cuts at: 513 1065 3398 513 Size: 552 2333 3074 Fragments arranged by size: 3074 2333 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3986 4003 4156 4341 4786 325 Size: 1255 2406 17 153 185 445 1498 Fragments arranged by size: 2406 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4205 5917 96 Size: 274 80 33 51 1443 2228 1712 138 Fragments arranged by size: 2228 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2072 2104 5919 5951 2072 Size: 32 3815 32 2080 Fragments arranged by size: 3815 2080 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5959 XbaI T'CTAG_A Cuts at: 3295 3295 Size: 5959 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2293 2293 Size: 5959 XhoI C'TCGA_G Cuts at: 1943 2804 2999 3110 1943 Size: 861 195 111 4792 Fragments arranged by size: 4792 861 195 111 XmaI C'CCGG_G Cuts at: 1870 2440 2458 2476 2489 2512 2533 2651 2896 Size: 570 18 18 13 23 21 118 245 Cuts at: 2896 3284 1870 Size: 388 4545 Fragments arranged by size: 4545 570 388 245 118 23 21 18 18 13 XmnI GAAnn'nnTTC Cuts at: 4088 4088 Size: 5959 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3767 276 Size: 53 83 186 936 2233 2468 Fragments arranged by size: 2468 2233 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspXI PstI PvuI SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: ApaI AscI AvrII BaeI BbvCI BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI NruI PacI PfoI PmlI PpuMI PspOMI PsrI PvuII RsrII SanDI SexAI SfiI SwaI