(Linear) (Six-base) MAPSORT of: KIBB9724.seq Check: 8166 from: 1 to: 1254 KIBB9724.orf With 182 enzymes: SgfI * December 5, 2014 12:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 702 1254 Size: 702 552 AfeI AGC'GCT Cuts at: 0 817 1099 1254 Size: 817 282 155 Fragments arranged by size: 817 282 155 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 613 645 1254 Size: 613 32 609 Fragments arranged by size: 613 609 32 ApaLI G'TGCA_C Cuts at: 0 267 1254 Size: 267 987 ApoI r'AATT_y Cuts at: 0 1237 1254 Size: 1237 17 AvaI C'yCGr_G Cuts at: 0 435 453 471 484 507 528 646 799 Size: 435 18 18 13 23 21 118 153 Cuts at: 799 891 994 1090 1105 1254 Size: 92 103 96 15 149 Fragments arranged by size: 435 153 149 118 103 96 92 23 21 18 18 15 13 BanI G'GyrC_C Cuts at: 0 285 421 1009 1254 Size: 285 136 588 245 Fragments arranged by size: 588 285 245 136 BanII G_rGCy'C Cuts at: 0 542 800 868 904 1043 1254 Size: 542 258 68 36 139 211 Fragments arranged by size: 542 258 211 139 68 36 BbeI G_GCGC'C Cuts at: 0 425 1013 1254 Size: 425 588 241 Fragments arranged by size: 588 425 241 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 120 154 707 741 1254 Size: 120 34 553 34 513 Fragments arranged by size: 553 513 120 34 34 BfrBI ATG'CAT Cuts at: 0 39 1254 Size: 39 1215 BglI GCCn_nnn'nGGC Cuts at: 0 440 458 476 512 533 633 1254 Size: 440 18 18 36 21 100 621 Fragments arranged by size: 621 440 100 36 21 18 18 Bme1580I G_kGCm'C Cuts at: 0 271 1254 Size: 271 983 BmgBI CAC'GTC Cuts at: 0 272 861 1254 Size: 272 589 393 Fragments arranged by size: 589 393 272 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1116 1254 Size: 1116 138 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 63 1254 Size: 63 1191 Bpu10I CC'TnA_GC Cuts at: 0 53 1037 1254 Size: 53 984 217 Fragments arranged by size: 984 217 53 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 868 955 1254 Size: 868 87 299 Fragments arranged by size: 868 299 87 BsaAI yAC'GTr Cuts at: 0 242 1254 Size: 242 1012 BsaHI Gr'CG_yC Cuts at: 0 236 422 763 1010 1254 Size: 236 186 341 247 244 Fragments arranged by size: 341 247 244 236 186 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 67 97 340 370 1254 Size: 67 30 243 30 884 Fragments arranged by size: 884 243 67 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 348 1012 1254 Size: 348 664 242 Fragments arranged by size: 664 348 242 BseYI C'CCAG_C Cuts at: 0 840 1013 1254 Size: 840 173 241 Fragments arranged by size: 840 241 173 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 58 1144 1254 Size: 58 1086 110 Fragments arranged by size: 1086 110 58 BsiEI CG_ry'CG Cuts at: 0 188 410 614 707 740 1254 Size: 188 222 204 93 33 514 Fragments arranged by size: 514 222 204 188 93 33 BsiHKAI G_wGCw'C Cuts at: 0 59 271 800 919 1043 1254 Size: 59 212 529 119 124 211 Fragments arranged by size: 529 212 211 124 119 59 BsmBI CGTCTCn'nnnn_ Cuts at: 0 148 1254 Size: 148 1106 Bsp1286I G_dGCh'C Cuts at: 0 59 271 542 800 868 904 919 1043 Size: 59 212 271 258 68 36 15 124 Cuts at: 1043 1254 Size: 211 Fragments arranged by size: 271 258 212 211 124 68 59 36 15 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 702 917 1254 Size: 702 215 337 Fragments arranged by size: 702 337 215 BsrFI r'CCGG_y Cuts at: 0 382 409 1254 Size: 382 27 845 Fragments arranged by size: 845 382 27 BstAPI GCAn_nnn'nTGC Cuts at: 0 923 1254 Size: 923 331 BstYI r'GATC_y Cuts at: 0 887 1254 Size: 887 367 BtgI C'CryG_G Cuts at: 0 188 281 602 672 740 1254 Size: 188 93 321 70 68 514 Fragments arranged by size: 514 321 188 93 70 68 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 180 1065 1143 1254 Size: 180 885 78 111 Fragments arranged by size: 885 180 111 78 EaeI y'GGCC_r Cuts at: 0 185 380 407 611 704 737 875 1254 Size: 185 195 27 204 93 33 138 379 Fragments arranged by size: 379 204 195 185 138 93 33 27 EagI C'GGCC_G Cuts at: 0 185 407 611 704 737 1254 Size: 185 222 204 93 33 517 Fragments arranged by size: 517 222 204 185 93 33 EarI CTCTTCn'nnn_ Cuts at: 0 142 1048 1254 Size: 142 906 206 Fragments arranged by size: 906 206 142 EcoICRI GAG'CTC Cuts at: 0 798 1041 1254 Size: 798 243 213 Fragments arranged by size: 798 243 213 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 63 1254 Size: 63 1191 HaeII r_GCGC'y Cuts at: 0 181 328 425 819 874 1013 1101 1254 Size: 181 147 97 394 55 139 88 153 Fragments arranged by size: 394 181 153 147 139 97 88 55 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 340 372 850 882 1254 Size: 340 32 478 32 372 Fragments arranged by size: 478 372 340 32 32 KasI G'GCGC_C Cuts at: 0 421 1009 1254 Size: 421 588 245 Fragments arranged by size: 588 421 245 MscI TGG'CCA Cuts at: 0 877 1254 Size: 877 377 MslI CAynn'nnrTG Cuts at: 0 199 622 1254 Size: 199 423 632 Fragments arranged by size: 632 423 199 MspA1I CmG'CkG Cuts at: 0 123 604 685 691 721 754 828 1254 Size: 123 481 81 6 30 33 74 426 Fragments arranged by size: 481 426 123 81 74 33 30 6 NaeI GCC'GGC Cuts at: 0 384 411 1254 Size: 384 27 843 Fragments arranged by size: 843 384 27 NarI GG'CG_CC Cuts at: 0 422 1010 1254 Size: 422 588 244 Fragments arranged by size: 588 422 244 NdeI CA'TA_TG Cuts at: 0 572 1254 Size: 572 682 NgoMIV G'CCGG_C Cuts at: 0 382 409 1254 Size: 382 27 845 Fragments arranged by size: 845 382 27 NotI GC'GGCC_GC Cuts at: 0 611 1254 Size: 611 643 NsiI A_TGCA'T Cuts at: 0 41 1254 Size: 41 1213 NspI r_CATG'y Cuts at: 0 627 1254 Size: 627 627 PasI CC'CwG_GG Cuts at: 0 207 1254 Size: 207 1047 PspXI vC'TCGA_Gb Cuts at: 0 799 994 1105 1254 Size: 799 195 111 149 Fragments arranged by size: 799 195 149 111 PstI C_TGCA'G Cuts at: 0 128 928 986 1254 Size: 128 800 58 268 Fragments arranged by size: 800 268 128 58 SacI G_AGCT'C Cuts at: 0 800 1043 1254 Size: 800 243 211 Fragments arranged by size: 800 243 211 SacII CC_GC'GG Cuts at: 0 605 1254 Size: 605 649 SapI GCTCTTCn'nnn_ Cuts at: 0 1048 1254 Size: 1048 206 SbfI CC_TGCA'GG Cuts at: 0 986 1254 Size: 986 268 SfcI C'TryA_G Cuts at: 0 124 924 982 1254 Size: 124 800 58 272 Fragments arranged by size: 800 272 124 58 SfoI GGC'GCC Cuts at: 0 423 1011 1254 Size: 423 588 243 Fragments arranged by size: 588 423 243 SmaI CCC'GGG Cuts at: 0 437 455 473 486 509 530 648 893 Size: 437 18 18 13 23 21 118 245 Cuts at: 893 1254 Size: 361 Fragments arranged by size: 437 361 245 118 23 21 18 18 13 SmlI C'TyrA_G Cuts at: 0 799 883 970 994 1105 1254 Size: 799 84 87 24 111 149 Fragments arranged by size: 799 149 111 87 84 24 SrfI GCCC'GGGC Cuts at: 0 455 473 530 1254 Size: 455 18 57 724 Fragments arranged by size: 724 455 57 18 StuI AGG'CCT Cuts at: 0 1111 1254 Size: 1111 143 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 67 99 1254 Size: 67 32 1155 Fragments arranged by size: 1155 67 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 288 1254 Size: 288 966 XhoI C'TCGA_G Cuts at: 0 799 994 1105 1254 Size: 799 195 111 149 Fragments arranged by size: 799 195 149 111 XmaI C'CCGG_G Cuts at: 0 435 453 471 484 507 528 646 891 Size: 435 18 18 13 23 21 118 245 Cuts at: 891 1254 Size: 363 Fragments arranged by size: 435 363 245 118 23 21 18 18 13 Enzymes that do cut: AarI AfeI AloI ApaLI ApoI AvaI BanI BanII BbeI BcgI BfrBI BglI Bme1580I BmgBI BplI BpmI Bpu10I BpuEI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrFI BstAPI BstYI BtgI BtgZI EaeI EagI EarI EcoICRI Eco57MI HaeII Hin4I KasI MscI MslI MspA1I NaeI NarI NdeI NgoMIV NotI NsiI NspI PasI PspXI PstI SacI SacII SapI SbfI SfcI SfoI SmaI SmlI SrfI StuI TstI XcmI XhoI XmaI Enzymes that do not cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI AscI AseI AsiSI AvrII BaeI BamHI BbsI BbvCI BciVI BclI BglII BlpI BmrI BmtI BsaI BsaBI BsaWI BsiWI BsmI BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtsI ClaI CspCI DraI DraIII DrdI EciI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI NcoI NheI NruI PacI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PvuI PvuII RsrII SalI SanDI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SspI StyI SwaI TaqII TatI Tth111I XbaI XmnI ZraI